..., Hess et al. successfully evolved wild type GFP to EGFP using CRISPR-X and subsequent FACS sorting. They...with unique tissue-specific promoters expressing GFP, tetracycline response elements, and shRNAs many ... of DsRed connected to a pH-sensitive variant of GFP (SEP) by a 9 amino-acid linker (see Figure 1). The...several GMAP-compatible vectors for a wide variety of biological applications. First, GMAP-compatible backbones...including a stop codon) formats. The collection is compatible with both standard Gateway cloning and the FNLCR...sites. For instance, the authors generated a GMAP-compatible backbone containing Rosa26 homology arms. The...GMAP, there will be a continuous expansion of compatible backbones, promoters, and genes available to ...

...England Biolabs website. For a comprehensive comparison to GFP, please refer to this table. Technology SNAP-tag...internal or surface proteins Single constructs are compatible with multiple applications (different fluorophores...

...find antibody plasmids for: Common antigens such as GFP or mCherry Monoclonals, Nanobodies, Sybodies, or ...peptide, from the popular SunTag system, fused to sfGFP for imaging. Learn more about antibodies and their... and Evolving Functional Proteins. METHODS: A Companion to Methods in Enzymology 8, 94–103 (1995). Carlos...

...pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2 CAG Archon1 EGFP Cre dependent 5 Boyden 115892 pAAV-Syn-Archon1-KGC-GFP-ER2 Syn Archon1...Archon1 EGFP Constitutive 8 Boyden 115893 pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2] Syn Archon1 EGFP Cre dependent...Podgorski Calcium Sensor: CaMPARI 100831 pENN.AAV.CAG.Flex.CaMPARI.WPRE.SV40 CAG CaMPARI none Cre dependent ...pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40 Syn CaMPARI2 his Constitutive 1 Schreiter 101061 pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV40...dependent 5 Looger 100832 pAAV.hSyn.CaMPARI.WPRE.SV40 Syn CaMPARI none Constitutive 1, 5, 9 Looger 101060 pAAV_hsyn_NES-his-CaMPARI2...F391W-WPRE-SV40 Syn CaMPARI2-F391W his Constitutive 1 Schreiter 101064 pAAV_hsyn_NES-his-CaMPARI2-L398T-WPRE-SV40...HaloCaMP1a 138327 pAAV-synapsin-HaloCaMP1a-EGFP Syn HaloCaMP1a EGFP Constitutive 1, 9 Schreiter Calcium Sensor...

...fluorescent protein that could be co-imaged with GFP using the same wavelength for excitation. The result... a good story (1). From a deep sea shrimp to a compact powerhouse of bioluminescence, NLuc is 100-fold...yield, brightness and improved maturation time compared to similar orange fluorescent proteins. In their...more than 2-fold brighter signal from Antares as compared to Fluc in this model. The construct for Antares...transfer of energy from one fluorescent protein to a compatible fluorescent protein. FRET can be measured by ...

...are generated by inserting a circularly-permuted GFP into an intracellular loop of various G-protein-coupled...enhanced brightness, expression, and sensitivity compared with earlier SF-iGluSnFR. Image from Aggarwal ...size and reduced sensitivity to pH and calcium compared to previous lactate biosensors, and its affinity...

...another marker, such as GFP. A researcher can use FACS to sort cells expressing GFP and later expand these...restriction sites but compatible with the Gateway® cloning system? Gateway® compatible vectors use recombination...generation or 3rd generation packaging system. For a comparison of the key differences between the 2nd and 3rd...lentiviral preference for transcribed genes. Studies comparing the lentivirus HIV and the retrovirus MMLV indicate...using this site include subcloning or appending compatible restriction sites onto the insert of interest...until the gene of interest is flanked by sites compatible with those in the vector into which one ultimately...

...SYFP2 Clover Green Mammalian Expresses Clover (a GFP variant) commonly used with mRuby2 pLSSmOrange-N1...to Amber via a 32 amino acid linker mGFP-10-sREACh-N3 Monomeric EGFP attached to super-REACh via a 10 amino...to target mTurquoise2 to various subcellular compartments pCEP4CyPet-MAMM Cyan Mammalian Expresses mammalian... Clover pGWF1 Cyan & Yellow Bacterial Gateway-compatible vector to express a gene of interest fused between...

...157970 pTR-UF11 chimeric CMV/Chicken Beta actin (CBA) GFP Control Sergei Zolotukhin Citation Information AAV2...intravitreal injection in mice and marmosets as compared to the AAV2 serotype. The AAV2(Y444F) serotype...in the retina following subretinal injection as compared to the parental AAV44.9 serotype. It was developed...acknowledge Shannon Boye and cite Boye, et al. 2016. Impact of Heparan Sulfate Binding on Transduction of Retina...

... pU6-sgGFP-NT1 Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GFP (NT1)...pSLQ1658-dCas9-EGFP Human expression vector containing dCas9 that is fused to 2x NLS and EGFP for CRISPR ...targeting endogenous CD71 gene 46919 pMLS-SV40-EGFP Target EGFP gene that is stably integrated into HEK293...complementary region. The uniqueness of CRISPRi, as compared to several recently published works on applying...

...Church lab uses this system to control expression of GFP.)  To turn this inducible system into a repressible... cells and ~24,000-fold in human HeLa cells as compared to basal expression. Co-expression of repressor...

...labeling. Size is also a factor. Bulkier tags, such as GFP, may interfere with protein expression or function...realizing it. Yet, this selection is crucial as it will impact downstream outcomes of an experiment, including...increased versatility in probe attachment, and compatibility with far-red and near-infrared fluorophores....Grimm & Lavis, 2022). These variations directly impact the way you can utilize these dyes. The most-used...may prove challenging to stain intracellular compartments in in vivo model systems, so extra care must...emission of labeled agonists and antagonists, impacting the interpretation of experimental results. It's...dopamine D2/D3 receptors (Allikalt et al., 2020) Impact of labeling process: The process of dye labeling...

...cassette comprised of OCT4, SOX2, LIN28, NANOG, and a GFP reporter. Minicircles are removed from cells by dilution...efficiency of reprogramming when compared to other methods, even when compared to integrating viral approaches...heterogeneous iPSC cell lines, which can complicate comparisons made between lines. Incomplete silencing of transgenes...and have a higher efficiency of generating iPSCs compared to other delivery methods. Retrovirus has a cloning...lentivirus has similar rates of reprogramming when compared to MMLV-derived retroviruses, it is not as well...heterogeneity between clones. This can complicate comparisons made between samples, i.e. transgene expression...Non-integrating methods have a smaller genetic footprint compared to integrating approaches. These methods eliminate...

...-positive assay) as well as stabilization (Nanog-GFP reporter activation and silencing of factor-linked... Listen to Our Tol2 Gateway-Compatible Toolbox Podcast Segment The impact of KLF4 N-terminal variants ...lead to comparable experimental results. Using PiggyBac transposons, the Woltjen Lab compared different...along with EGFP while retaining an emission spectrum that is easily separable from that of EGFP. This makes...latest hot plasmid updates!     Tol2 Gateway-compatiable toolbox for studying the nervous system The... Cole lab recently deposited the Tol2 Gateway-Compatible Toolobox, which will allow scientists to quickly... neuorodgenerative diseases. The Tol2 Gateway-Compatible Toolbox is based on the original Tol2kit generated...

...marker like GFP, researchers have turned dCas9 into a customizable DNA labeler compatible with fluorescence...typically used for gRNA May contain reporter gene (e.g. GFP) to identify and enrich positive cells, or selection...transfer vectors May contain reporter gene (e.g. GFP) or selection marker to identify and enrich positive...detecting multiple genomic loci, and compatible with live cell imaging. Compared to techniques like fluorescence...nuclease pair for every genomic target. Due to its comparative simplicity and adaptability, CRISPR has rapidly...it meets two conditions: The sequence is unique compared to the rest of the genome. The target is present...gRNA targeting sequence is for the genomic target compared to the rest of the genome. Ideally, a gRNA targeting...

...marker like GFP, researchers have turned dCas9 into a customizable DNA labeler compatible with fluorescence...typically used for gRNA May contain reporter gene (e.g. GFP) to identify and enrich positive cells, or selection...transfer vectors May contain reporter gene (e.g. GFP) or selection marker to identify and enrich positive...detecting multiple genomic loci, and compatible with live cell imaging. Compared to techniques like fluorescence...nuclease pair for every genomic target. Due to its comparative simplicity and adaptability, CRISPR has rapidly...it meets two conditions: The sequence is unique compared to the rest of the genome. The target is present...gRNA targeting sequence is for the genomic target compared to the rest of the genome. Ideally, a gRNA targeting...

...plasmids allow cloning of protein coding sequences with GFP-LOVpep, cpPDZ, ePDZb and ePDZb1 as tags. pDawn/pDusk...H134 Widely used variant with larger photocurrent compared to CrChR2 450 ChETA E123T mutation; creates faster...applying optogenetic tools derived from direct comparative analysis of microbial opsins. Nat Methods. 9(...

.... All vectors include the germline expression compatible tbb-2 3’UTR. For both the eft-3 and hsp-16.48...generated variants with and without a C-terminal EGFP fusion. For the sgRNA, vector pMB60 allows in vitro...47944 pMB62 : Expresses C. elegans optimized Cas9::mEGFP from the eft-3 (eef-1A.1) promoter. 47945 pMB63 ...47946 pMB66 : Expresses C. elegans optimized Cas9::mEGFP from the hap-16.48 promoter. 47947 pMB67 : Expresses...

...biological properties of new fusions can be compared with mEGFP or mTurquoise2, which are established true...fluorescent protein. In the jellyfish Aequorea victoria, AvGFP forms a homodimer. In corals, the red fluorescent...detect weak dimerization tendency, as is present in EGFP. Constantini et al, (2012) developed a cell-based...fluorescent protein is monomeric without the accompanying data to prove it is worthless. The OSER assay...that you will be using. If you do a head-to-head comparison right, it will be valuable information for the... are engineered can be shared to simplify the comparison by others. Together, we can build a valuable ...

...enhancer regions (Mendenhall et al., 2013). By comparing gene activation when enhancers were active or ...increased gene activation at the targeted site compared to other Tet1 delivery systems. Transcriptional...) Vlatka Zoldoš’ lab has deposited pdCas9-DNMT3A-EGFP and pdCas9-DNMT3A-PuroR for targeted cytosine methylation...methylation in mammalian cells. Co-expression markers EGFP and PuroR enable sorting and selection of transduced...Goodell's lab has deposited pcDNA3.1-dCas9-MQ1(Q147L)-EGFP, a fusion of dCas9 to a small DNA methyltransferase...epigenome-editing tools. pLV hUbC-dCas9-MQ1(Q147L)-EGFP is also available for lentiviral transduction. Lysine-specific...