...December 2011 Newsletter Overview of TALEN Technology Imagine being able to manipulate a specific region of DNA...

...Enzyme collection contains enzyme genes for fixing damaged and degraded DNA prior to amplification, enzymes...

...application TMN200 (200mM sodium chloride, 1mM magnesium chloride, 20mM Tris at pH 8.0) Suitable for in...

...corresponding catalog item (see maps section for images). AAV Pro cells were transduced with either pAAV-Ef1a-mCherry-IRES-Cre...

...gene regulatory element; NHP, non-human primate. Image courtesy of the Gradinaru Lab and the Caltech CLOVER...

...6xTRE-FLEX-hM4Di.mCherry hM4D (Gi) Tet-inducible mCherry Yes Talmage 177672 pAAV-nEF-Con/Fon DREADD Gi-mCherry hM4D ...

...SARS-CoV-2 is primed by the serine protease TMPRSS2. Image credit: Maya Kostman for the Innovative Genomics...

... information, prenatal LMD microarray data, ISH image data, and anatomic reference atlases of prenatal...

...!), photoactivatable tags for super resolution imaging, epitope tags like His for easy western blotting...

...none Flp dependent 8 Deisseroth 158757 AAV-CAG-SomaGCaMP6f2 CAG soma-GCaMP6f2 none Constitutive 8, 9 Boyden...

...activation Magnus et al, 2019 PSAM Q79G,Q139G Gly PSEM 22S Cl- influx Neuronal inhibition Magnus et al, 2011...activation Magnus et al, 2011 PSAM L141F,Y115F Gly PSEM 89S Cl- influx Neuronal inhibition Magnus et al, ...uPSEM 792, uPSEM 817 Cl- influx Neuronal inhibition Magnus et al, 2019 PSAM4 5HT3 Varenicline, uPSEM 792, ...5HT3 PSEM 89S Na+, K+ influx Neuronal activation Magnus et al, 2011 Browse Addgene's collection of PSAM...Natl. Acad. Sci. USA 106:19197–202. PMID: 19858481 Magnus CJ, Lee PH, Atasoy D, Su HH, Looger LL, and Sternson...interactions. Science 333:1292–96. PMID: 21885782 Magnus CJ, Lee PH, Bonaventura J, Zemla R, Gomez JL, Ramirez...

...targeted genomic loci. CRISPR imaging has numerous advantages over other imaging techniques, including that...cell imaging. Compared to techniques like fluorescence in situ hybridization (FISH), CRISPR imaging offers...repress target genes, purify specific regions of DNA, image DNA in live cells, and precisely edit DNA and RNA...chromatin dynamics in living cells. Multicolor CRISPR imaging allows for simultaneous tracking of multiple genomic...stability and enable a signal amplification for better imaging of genomic loci. The fluorescent CRISPR system ...used to modulate RNA editing , for in vivo RNA imaging, or to control alternative splicing. Learn more...RNA scaffolds for signal amplification in genome imaging.. 2018. Ma H, Tu LC, Naseri A, Chung YC, Grunwald...

...agarose or magnetic beads. Using centrifugation (for agarose beads) or a magnet (for magnetic beads), collect...a substrate kit. Image the membrane using either X-ray film in a darkroom or an image capture machine....reaction can be detected either digitally by an imaging machine or by X-ray film. HRP cannot be used to...are two primary methods used, using agarose or magnetic beads, but both rely on the same general strategy...antibody, incubate, and wash. Preserve sample and image using a microscope. Special considerations Many ...

...gene of interest (YGOI) into a recipient plasmid. (Image from Plasmid 101: Restriction cloning ) Back to ...Gateway® Recombination Cloning Gateway cloning (Image from Plasmid 101: Gateway Cloning ) Gateway® cloning...gateway cloning. Back to Top TOPO® Cloning TOPO (Image from Plasmid 101: TOPO Cloning ) Toposiomerase based...chewed back by the 5' exonuclease. Gibson Assembly (Image from Plasmid 101: Gibson Assembly ) Back to Top ...your next cloning project. Golden Gate Cloning (Image from Plasmid 101: Golden Gate Cloning ) Back to ...

... at Addgene. Sherf, et al. 1994. Promega Notes Mag. Giannakis, et al. BioTechniques. 2003. PubMed ...Nano-lanterns for multicolor luminescence imaging  Fluorescent imaging techniques have become indispensable...):198-200. SunTag system for single molecule imaging and inducible gene expression Brilliant technologies...have adopted and employed this concept in both imaging and inducible gene expression studies, which are...advanced technology in the fields of single molecule imaging and inducible gene expression by creating the SunTag...system, cleverly termed TRICK, for Translation RNA Imaging by Coat protein Knock-off, is made up of three ...developed a CRISPR-based multicolor tool enabling the imaging of multiple endogenous genomic loci simultaneously...

...x-ray, or a biologist imaging the smallest structures of life; the key to a great image is contrast. The human...Epi-fluorescence microscopes. (See left image above for an example image). Point Scanning Confocal Microscopy...the speed at which a confocal image can be acquired. Assembling an image pixel by pixel is slow; therefore... challenging samples to image as they require both optical sectioning, imaging over large distances and...can push imaging depths closer to 1 mm. Traditionally, 2-photon has been a very slow imaging technique...Lightsheet designs that allow for multi-view imaging can image thick scattering samples in their entirety...photobleaching when acquiring multiple images over a large axial range. 2-photon imaging, which restricts excitation...

...open the offending red-green image, go to Image>Color>Replace Red with Magenta. Or you can use Color>Split...wash again, then image. But here’s where the “sequential” part comes in. After you image the first target....1 (magenta), anti-AnkyrinG (green), and Hoechst nuclear stain (cyan). Panels C1-C3 show magnified detail...ve captured your images, various software programs will allow you to align the images of each labeled ...maybe you just want to make some really beautiful images? Multiplex IF may be just what you need! How does...of primary and secondary antibody labeling and imaging, repeating for each target. Alternatively, you ...Sequential Multiplex IF, where one target is labeled and imaged at a time, inactivating the fluorescent secondary...

...resolution imaging, where the power of the technique relies heavily on the same fluorophore being imaged multiple...order to reconstruct an image. The best PA-FPs used for super-resolution imaging usually have the following...Fluorescent Protein Imaging Choosing Your Fluorescent Protein for Multi-Color Imaging Catch Up on Your GFP...would otherwise be missed with conventional FP imaging. PA-FPs are divided into 2 main categories - those...photoactivation - and have enabled sophisticated imaging techniques.     Irreversible photoactivation Irreversible...phototoxic to cells, most scientists working on in vivo imaging models perfer the PA-RFPs over PA-GFP. Another ...fluorescent state make them useful for 2-color imaging with green PA-FPs. Table 2: Properties of Selected...

...Individual images as well as collections of images are easy to download in a .png file format. Images are under...indicate if changes were made to the images. This requirement means the images are probably better suited for...than for journal publications. Google Image Search Google image search is a great tool for searching the...can search for images “Labeled for reuse with modification.” Occasionally Google Image Search results ...results includes images that can’t be reused without attribution, despite filtering for images labeled for reuse...your public library. If you want to use pre-drawn images in your figures Biorender Biorender is like clip...create it. Additionally, you can upload your own images to the Biorender workspace. Free Biorender accounts...

...named it Magneto 2.0. Using Magneto2.0-p2A-mCherry constructs, the lab verified that Magneto2.0 was magnetically...revealed that Magneto 2.0 allows for magnetic remote control of neuronal activation. Find Magneto 2.0 plasmids...is multiplex automated genome engineering (MAGE) (1). MAGE uses recombineering (2) to simultaneously incorporate...use the MAGE method in unmodified bacterial species (4). This set of plasmids (dubbed pORTMAGE) expresses.... Methods 2016. PubMed PMID: 27798611 Magneto 2.0 - A magnetic remote control for the nervous system ... 74308 pcDNA3.0-Magneto2.0-p2A-mCherry Mammalian Expression 74334 pCR8-Magneto2.0-p2A-mCherry Gateway...Gateway 74333 pCR8-Magneto2.0 Gateway 74307 pAAV-CMV-DIO-Magneto2.0-sNRPpA AAV 74306 pAAV-CMV-DIO-TRPV4...