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Showing: 41 - 60 of 757 results

  1. FlyCRISPR

    Type
    Collection
    ... CRISPR Protocols gRNA Design Tools CRISPR Blog Posts Harrison Lab - Wildonger Lab - O'Connor-Giles...

  2. CRISPR Plasmids - Tagging

    Type
    Collection
    ...Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR system has been adapted to allow tagging...using them, are described below. Read our CRISPR 101 post for more information about HDR/repair template design...

  3. General Transfection

    Type
    Protocol
    ...may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Viral Service Introduction This...vectors) Day 1 (pm): Transfect Cells Day 2 (am): 18h post transfection - Remove media, replace with fresh ...

  4. AAV ddPCR Titration

    Type
    Protocol
    ...AAV Viral Service Viral Vector Guides Virus Blog Posts Molecular Biology Reference Introduction This protocol...tips on AAV titering using ddPCR, read this blog post on ddPCR for AAV quantification. This protocol was... tips on AAV titering using ddPCR, read our blog post....

  5. Luciferase Plasmids

    Type
    Collection
    ...about DULIP in our 27 Hot Plasmids from 2016 blog post. LuTHy plasmids (Double‐Readout Lu minescence‐based...in our 28 Hot Plasmid Technologies from 2015 blog post. RNA Biology CMV-LUC2CP/intron/ARE, CMV-LUC2CP/ARE... STARR-seq in our 27 Hot Plasmids from 2016 blog post. pGL3-lRF , pGL3-LRR : Genomic tiles spanning a ...screening of transformants in cloning process. Lynne Postovit 119760 pSGDlucV3.0 Firefly/Renilla Insertion of...

  6. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ... CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR revolution shows no signs of slowing ...researchers to further develop the CRISPR toolkit by posting lab protocols , providing tips from experts in ...check out Addgene’s CRISPR Guide and CRISPR blog posts , or find CRISPR plasmids for your research. Addgene...Scientists continue to wrestle with the possibility of postnatal and germline editing, with the International Summit...

  7. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...protocol for coronaviruses (184 KB) Addgene Blog Posts Viral Vectors 101: Pseudotyping Antibody Neutralization...

  8. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Two classes of base editors-- cytosine base editors...

  9. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR plasmids have been designed...

  10. Jaenisch Lab CRISPR Plasmids

    Type
    Collection
    ... CRISPR Protocols gRNA Design Tools CRISPR Blog Posts CRISPR-on is an RNA-guided transcriptional activator...

  11. AAV Made on Request

    Type
    Collection
    ...timeline for when this service will resume, but we will post updates on this page. Please email help@addgene....

  12. Lentivirus Production

    Type
    Protocol
    ...(pm): Transfect packaging cells Day 2 (am): 18 h post-transfection. Remove media, replace with fresh media...cells. Virus can be harvested at 48, 72, and 96 h post transfection in individual harvests or a combined...

  13. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR plasmids have been designed...

  14. Retrovirus Plasmids

    Type
    Collection
    ...GFP; Also see pMIG-w , a variant that has WRE, a post-transcriptional regulatory element that increases...

  15. Antibody Production

    Type
    Collection
    ... Protocols Recipient Instructions Antibody Blog Posts Addgene Help Center...

  16. CRISPR Plasmids - Yeast

    Type
    Collection
    ...Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR plasmids have been designed...

  17. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Prime editing is a “search and replace” gene editing...

  18. Microbiology Resources

    Type
    Collection
    ...CRISPR Methods for Bacterial Genome Engineering blog post to see how Addgene depositors are making this possible...

  19. Worm Expression Resources

    Type
    Collection
    ...elegans . Return to top Other Resources Addgene Blog Posts An “elegans” Approach to Better CRISPR/Cas9 Editing...
Showing: 41 - 60 of 757 results