...manipulate cells and their governing biomolecular processes....

... second guide within a U6-guide cassette. This process is compatible with all pX458 family plasmids. Additional...

...the user through the REAL or REAL-Fast assembly process or lists of plasmid ID numbers required for FLASH...

... may not be able to accept all orders. We will process requests in the order received. FAQ Which plasmids...

...pathways that are important in cellular recognition processes. Laboratories interested in these approaches have...

...SynBio Deposit Guide or start our online deposit process . Browse SynBio Plasmids by Category Cloning and...

...antibody production methods and quality control processes for purified recombinant antibodies....

...Förster resonance energy transfer, or FRET , is a process by which energy is non-radiatively transferred ...

...screen for genes involved in specific biological processes. For more information, see our Guide to Plasmid...

...disease and implicate a wide range of cellular processes. The vast majority of cases (90-95%) of ALS are...

...1, which may require additional lab biosafety procedures. Additionally, because this virus integrates ...

...artificially activate or inhibit specific biological processes. Addgene’s collection contains two main classes...

...conventional HDR-mediated gene knock-in. The detailed procedure can be found in Sakuma et al., Nature Protocols...

... one-enzyme RT-qPCR assay for SARS-CoV-2, and procedures for reagent production. Bhadra S, et al. bioRxiv...

...families, HDR oligonucleotide design, and batch processing for designing genome-wide gRNA libraries. PubMed...

... tags can be used to simplify the purification process. Using plasmids in this collection, you can highly...

...neurons with vesicular stomatitis virus vectors. Proc Natl Acad Sci USA 108, 15414–15419. PubMed PMID:...Further, the connections between regions are often reciprocal, forming a neuronal loop which is activated and...

...output from the first process to the second process. For example, our bbduk process created trimmed FASTQ...pipeline, we wrote a separate Nextflow process. In Nextflow, a process defines which command or script will...specifying the output of an initial process as the input to a subsequent process, we linked each step in our ...pipeline steps into processes and then connect the steps using the output from one process as input to the... Nextflow process.  Next, we specified the input and output channels for the initial process and tested...was clear: to build one process at a time and connect each subsequent process to the existing ones. Once... our first process was working as expected, we moved forward with creating the next process and linking...

...receptors potently activated by an inert ligand. Proc. Natl. Acad. Sci. USA 104:5163–68. PMID: 17360345...with a specifically designed Gi-coupled receptor. Proc. Natl. Acad. Sci. USA 95:352–57. PMID: 9419379 Davies...G protein regulation of β cell function in vivo. Proc. Natl. Acad. Sci. USA 106:19197–202. PMID: 19858481...to be involved in a wide variety of biological processes, including initiating signalling pathways in inflammation...

...you, while the process has become much more refined and user friendly, to own the process entirely remains...quality data! Addgene's new in-house NGS process To start, the process is only made possible by the use of ... we are able to take greater ownership of this process with in-house sequencing! In addition to sequencing...of our high-throughput DNA isolation process that yields high-quality isolated DNA samples of sufficient...sufficient quantity for sequencing. This process is completed in a plate format yielding anywhere from two to... of 96 samples per week. This is where our new process will begin. Partnering with seqWell, we are using.... From start to finish the library preparation process only takes about one day, even for six plates (...