... that specialty, who may be other scientists, or science journalists, or policy makers. The term editor...transcription during vertebrate embryogenesis. My interests, though, have proven unruly. Instead of settling...In my role as an editor at Science, my various interests come together as useful and productive, forming...forming a different constellation of interests and skills than what fits for applied research. I focus in ...meetings, societies, networks just as do research scientists. There we meet others in the scientific publishing...more planets to choose from. Put your skills and interests into your backpack, and step out there into the...the galaxy. TL;DR: Many roads need scientists open for adventure. Many thanks to our guest writer, Pamela...

...Scientific Domains Tweeted by Biologists. 7. Biologists, neuroscientists and ecologists (re)tweet scientific domains...time; however, ~20% of life scientists  (biologists, neuroscientists, ecologists) tweet scientific domains...Categorized by Discipline. Biologists, neuroscientists, and ecologists (orange box) post tweets containing...red box). 8. Scientists tend to follow other scientists within their fields than scientists outside their...orange)  scientists largely cluster by discipline, suggesting that scientists tend to follow scientists within...analysis of scientists on Twitter.” This paper addresses the following questions about scientists on Twitter...al. 2017. 1. Among scientists, historians have the most Twitter users. Biologists rank #8. Figure 1:...

...Krogan at UCSF we’ve brought together a network of scientists from more than 20 labs to form the QBI Coronavirus...of our paper, our group has been inundated with requests to share our plasmids with other researchers. ...to see so much interest, because it means more scientists will be working on this problem more quickly....the US and Canada. In fact, we received so many requests (175 and counting) that we ran out of FedEx supplies...administrative headache receiving constant email requests and tracking their fulfillment manually in a shared...over the distribution to expand the network of scientists working on this even more quickly.  And, if you... making it accessible to all people, not just scientists! *If you are requesting plasmids for coronavirus...

...that explored how scientists connect on Twitter that I realized 1) a lot of scientists are using social... "5 Ways Scientists SHOULD Be Using Twitter" podcast from Hello PhD In this podcast the hosts, Josh and... what percent of scientists and engineers regularly use the platform, why scientists are using the platform...about online social networking for scientists Learn why scientists should be on Twitter Learn about using... it was cool to share my knowledge with other scientists who maybe hadn’t used this method. I enjoyed ...about new scientific developments and helping scientists is a win-win for me. By using Twitter, I found...consistent writing for the Addgene blog (~1-2 blog posts per month) and use of Twitter and LinkedIn probably...

...Career Coaching can be an excellent tactic for scientists making a career shift or who wish to improve ...coaches have experience and knowledge to help scientists transition out of the academic sphere into a ...and at scientific conferences, I see that few scientists are aware of the benefits of coaching relationships...invest in this. However most coaches who focus on scientists are sensitive to this and have coaching plans...find a coach? Start with referrals. Are there scientists you know who have had a successful coaching experience...outcomes? Ask openly for clear information on costs. Can you suggest some coaches? Yes I can. Please... very effective and also help keep the coaching costs low. Most will work with you remotely (by phone,...

...operating.  Managing scientists I should add a few special words about managing scientists because, let’s face...the first in a 5 part series on Management for scientists.  “I'm slowly becoming a convert to the principle...special. Below are some special characteristics of scientists in the work place as described by Sebastiano ...I think this is true of most people, not just scientists) Are self-directed, but need precise leadership...flexibility, sometimes taken to an extreme by scientists) Are highly mobile and can move to a new workplace...series on Introduction to Managing People for Scientists. Subscribe to the Addgene Blog to follow the ... management for scientists...

...the most recent plasmid blog posts Find the most recent CRISPR blog posts Resources at Addgene Browse...page. So let’s get to it. Here’s the top 10 blog posts from the year. 10. Hot Plasmids - March 2019 - Anti-CRISPR... the diversity of tools we share on behalf of scientists. 9. Plasmids 101: Positive and Negative Selection...ve enjoyed going through some of these top blog posts. We’d also like to use this time to hear some feedback...

... AAV9 Biosensor St-Pierre New viral vector pAAV-hSyn-JEDI-2P-WPRE AAV9 Biosensor St-Pierre New viral... constitutively express JEDI-2P, deposited by the St-Pierre lab. Also of interest is the Tian lab's pAAV_hSynapsin_psychLight2...New serotype pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST AAV9 Optogenetics Adesnik New viral vector pAAV-nEF-Con...

...bloggers Roodolph (Roo) P. St Pierre and Rose C. St Pierre! Roodolph (Roo) P. St Pierre is currently a doctoral...contributed by guest bloggers Roodolph (Roo) P. St Pierre and Rose C. St Pierre, founders of STEAMid. Internships.... Roo is the co-founder of STEAMid.       Rose C. St Pierre is currently a graduate student at Northeastern...paint a comprehensive picture of your academic interests, potential, and drive. Read the associated prompt...Thus these noble individuals are inundated with requests. The easier you make it for them, the likelier...researchers Applying for jobs? Check out these blog posts  ...

...Plasmid requests correlate with paper publication The dataset includes 11,426 plasmid requests over 6 ...plasmids and a total of 2–2,590 requests. We received plasmid requests from 56 countries, though the data...plasmid requests correspond to publications of popular papers. Figure 1: Plasmid requests by date...significant fraction of the workforce. Wet lab scientists are no exception to this effect -- meticulously... chose to examine a dataset of Liu lab plasmid requests from Addgene, as a proxy for global activity in...was provided by Addgene on 5/26/20 and contains requests from 5/22/14 to 5/19/20). Each paper has between...Rough estimation of COVID-19 effect on plasmid requests Before we began any sophisticated analyses, we...

...fourth post in the Addgene Blog Mentoring for Scientists Series. I have been thinking a lot about Mentoring...Mentoring for over 10 years. Many successful scientists describe having a “posse” of mentors as one key ... Stories and Strategies from Successful Women Scientists” by Ellen Daniell. In “Every Other Thursday,”...,” Daniell describes a group of 7 women scientists who met every other Thursday for 25 years. They helped...or to be read or watched during the meeting.  Scientists do better with process, so don’t just get together... style (science). Have a potluck dinner. Invite guests from a lab, career or industry of interest – learn...with any questions about mentoring. Read the other posts in the series to get more advice on mentoring: ...

...the 5 scientists who were awarded the Addgene-Seeding Labs Plasmid grant this year. These scientists work... Accelerating science and supporting scientists globally are the shared mission of both Seeding Labs ...potential of Cry proteins in order to manage insect pests and to support sustainable agriculture. For this...then be screened for toxicity against local insect pests. By developing biological agents such as insecticidal...happy to be able to support diverse groups of scientists working in a wide range of research fields and...

...real time (St. Martin et al., 2018). The ACE reporter is a bicistronic construct that consists of a mutated...mediated editing (ACE) reporter in action. Image from St. Martin et al., 2018. APOBEC-Cas9n-UGI targets...been successfully edited with APOBEC3A and APOBEC3B (St. Martin et al., 2018). TLDR: The ACE reporter from...which is not ideal for monitoring single base edits (St. Martin et al., 2018). To circumvent sequencing and... of APOBEC-Cas9 editisome complexes in real-time (St. Martin et al., 2019). To create the eGFP reporters...efficiencies using base editing reporters. Image from St. Martin et al., 2019. The Harris lab used these...Reports 9: . https://doi.org/10.1038/s41598-018-36739-9 St. Martin A, Salamango D, Serebrenik A, Shaban N, Brown...

...2022 Aug 16. pii: S0092-8674(22)00916-3. Francois St-Pierre Voltage Voltage Sensitive Fluorescent Protein...;75(5):779-85. Vincent Pieribone Voltage ArcLight-ST to detect subthreshold membrane potential changes...Photosynthesis-dependent H 2 O 2 transfer from chloroplasts to nuclei provides a high-light signalling mechanism...

...500,000x Thank You! Addgene's new location: 75 Sidney St, Suite 550A, Cambridge, MA 02139 Have a question?...! That's a 1/2 million plasmids sent to happy scientists around the world in ~10 years! We're extremely...extremely proud to be able to support scientists by bringing you all the plasmids you need. We're focused on...world. We've shipped plasmids to more than 90,000 scientists at 5,200 institutions in 82 different countries...distribution has grown. Additionally, more and more scientists outside of the US now use the repository – about... so excited to continue to improve access for scientists everywhere to get the tools they need to progress...to ship plasmids, analyze QC sequence, answer scientists' questions, manage our website, and continue ...

... Y, Oyama K, Ji B, Takahashi M, Huang X-P, Slocum ST, DiBerto JF, Xiong Y, Urushihata T, Hirabayashi T...respond to a different ligand, Salvinorin B (SALB). Scientists have shown that both KORD and hM3Dq can be used...PSEMs (or uPSEMs), have also been developed as agonists for PSAM4. A given PSAM is coupled with an ion...controlled with cell-type specific promoters. Table 3 lists some common promoters found in chemogenetic receptor...PMID: 22821234 Roth BL (2016). DREADDs for Neuroscientists. Neuron 89:683–694. PMID: 26889809 Small KM...

...meningitidis (NM) 3' NNNNGATT Streptococcus thermophilus (ST) 3' NNAGAAW Treponema denticola (TD) 3' NAAAAC Additional...CRISPR-Display. 2015. Shechner DM, Hacisuleyman E, Younger ST, Rinn JL. Nat Methods . 12(7):664-70. PMID: 26030444...transcription-activator-like effector nucleases (TALENs) required scientists to design and generate a new nuclease pair for...sites throughout the genome where partial homology exists. These sites are called off-targets and need to...

...2022.02.13.480251 JEDI-2P voltage sensor The François St-Pierre lab and colleagues have developed a new genetically-encoded...pAAV-CAG-dLight1.2 (AAV1) pGP-AAV-syn-FLEX-Ace2N-4AA-mNeon-ST A122D WPRE (AAV1) pAAV-hSynapsin1-axon-GCaMP6s-P2A-mKate2...plasmids is our Fluorescent Biosensors Collection - scientists are always coming up with new tools to measure...

...it? Howarth: Our collaborator Uli Schwarz-Linek at St. Andrews discovered the domain. It is small, highly...

...have decided to run as The Green Lantern’s friend, St Walker: The Blue Lantern. While The Green Lanterns...