...you choose and use antibodies, with an expert technical support team ready to answer your questions. All...antibody production and storage techniques, and explain common applications. View Guide ...

... genes in zebrafish (Hwang and Fu et al., Nat Biotechnol. 2013). The Joung lab has also modified their...use in mammalian cells SpCas9 variants with no detectable off-target effects Modified PAM specifity of ...

...Vector Resources Addgene's Data Hub : Curated technical reports from the research community demonstrating...troubleshooting advice and explanations of viral vector technologies. Penn Vector Core AAV Vectors : Many AAV vectors...

...inclusive, ‘open-access ethos’ to the results, techniques and reagents with the highest-possible quality... research as well as applied research in small biotech and pharmaceutical companies. The RESOLUTE project...

...pmax-DEST [48222] and (Link opens in a new window) Lifetechnologies Gateway Destination Vectors ) to adapt to ...pmax-DEST : Gateway Destination vector derived from Clontech's pmax (CAGGS) expression vector....

... by Sypro Ruby staining In-vitro transduction (detected by fluorescent expression when applicable) PCR-based...plasmid as viral particles. A member of Addgene’s technical support team will contact you shortly with the...

...plasmids in the future. AVEXIS: One popular method to detect interactions is AVEXIS (for Avidity-based extracellular...Interaction Screening (AVEXIS) for the scalable detection of low affinity extracellular receptor-ligand ...

...intellectual property rights, and provides liability protection for material providers. Addgene’s deposit agreements...distribution. Please see the Sample Agreements on our Technology Transfer Information page for more information...

...son of sevenless homolog 2 (Drosophila) FLJ25596 TEC tec protein tyrosine kinase MGC126760, MGC126762, PSCTK4...subfamily 4, group A, member 3 CHN, CSMF, MINOR, NOR1, TEC NR5A1 nuclear receptor subfamily 5, group A, member...potential pathogens each day. Their ability to protect against infection depends on two systems that work... have been infected by a foreign pathogen. They detect infected cells via receptors such as KIR and NKG2D... (C-C motif) ligand 25 Ckb15, MGC150327, SCYA25, TECK CCL26 chemokine (C-C motif) ligand 26 IMAC, MGC126714...

...CRISPR Guide for a general introduction to CRISPR technology. Check out our CRISPR reference page for CRISPR...resources Bacterial CRISPR Resources The CRISPR technology was developed from bacterial immune systems, ...

...CRISPR Guide for a general introduction to CRISPR technology. Addgene's worm CRISPR resources include plasmids... website . Co-CRISPR plasmids that facilitate detection of genome-editing events. Developed in Craig Mello's...

...neurons function. They are also delving into new technologies and resources in order to gain more fundamental...mitochondrial DNA using mitoBEs . Yi et al. Nat Biotechnol. 2023 May 22. See More AAV Viral Preps Find AAV...

... used with 2nd and 3rd generation lentivirus technologies. ID Plasmid Generation Type Description PI 12253... used with 2nd and 3rd generation lentivirus technologies. Looking for lentiviral plasmids to express ...

...traditional tagging methods. Several different tagging techniques, as well as the plasmids and protocols needed...Förstemann lab has developed a CRISPR tagging technique for use in Drosophila cells that uses PCR to generate...

...zebrafish ( Sander et al., Nat Biotechnol. 2011 ; Huang et al., Nat Biotechnol. 2011). The table below lists...

...Fluorescent Proteins Blog: Luciferase 101 Blog: Technologies Enabled by NanoLuc® Luciferase Blog: Luminescent...more about pHlash sensor in our 28 Hot Plasmid Technologies from 2015 blog post. RNA Biology CMV-LUC2CP/...

...Center at UCSF / QB3 Wiki Stepanenko et al. : BioTechniques, Vol. 51, No. 5, November 2011, pp. 313–327 ... No. 10, pp.1296-304 Kogure et al. : Nature Biotechnology, May 2006, Vol. 24 No. 5, pp.577-81 Shcherbo...

...avidity-based extracellular interaction screen to detect protein interactions, where the extracellular regions...

...opens in a new window) based on yeast display technology (Link opens in a new window) and an optimized...

...wild-type FokI nuclease domains ( Sander et al., Nat Biotechnol. 2011 ): ID Plasmid C-terminal 0.5 TALE repeat...