We narrowed to 68 results for: ANG

...Addgene Zhang Lab CRISPR Resources Tips for and FAQs about the Zhang Lab CRISPRs Browse All Zhang Lab Plasmids... Genome Engineering CRISPR Zhang Lab CRISPR Plasmids Zhang Lab CRISPR Plasmids...sequences Zhang Lab SAM Cloning Protocol 321.5 KB Return to top AAV - In vivo Genome Editing The Zhang Lab ... Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F. Science . 2013 Jan 3. DOI: 10.1126...S, Trevino AE, Scott DA, Inoue A, Matoba S, Zhang Y, Zhang F. Cell . 2013 Sep 12;154(6):1380-9. doi: 10.1016...Heidenreich M, Xavier RJ, Langer 9, Anderson DG, Hacohen N, Regev A, Feng G, Sharp PA, Zhang F. Cell . 2014 Oct...plasmids from the Zhang lab Genome...

...Browse the Zhang lab’s comprehensive human transcription factor collection, available at Addgene. The... Depositor Collections MORF Feng Zhang - Multiplexed Overexpression of Regulatory Factors...Factors (MORF) Collection You may also like... Feng Zhang Lab Plasmids The Human TFome Library Stem Cells ...Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F. Cell 2023 Jan 5; 186(1):209-229. PubMed (Link...Description MORF library 137000, 192821, 1000000218 Zhang A collection of human transcription factor open ...

...PMID: 33230293 Yang, S., Zhang, Y., Xu, J., Zhang, J., Zhang, J., Yang, J., Jiang, Y., & Yang, S. (2021)....28931002 Cheng, A. W., Wang, H., Yang, H., Shi, L., Katz, Y., Theunissen, T. W., Rangarajan, S., Shivalila, ...Tian, R., Huang, Z., Jin, Z., Li, L., Liu, J., Huang, Z., Xie, H., Liu, D., Mo, H., Zhou, R., Lang, B., Meng...PMID: 32217751 Wang, H., Yang, H., Shivalila, C. S., Dawlaty, M. M., Cheng, A. W., Zhang, F., & Jaenisch...e04766. PMID: 25497837 Ma, Y., Zhang, J., Yin, W., Zhang, Z., Song, Y., & Chang, X. (2016). Targeted AID-mediated... Q., Xie, Z., Bai, M., Dong, R., Tang, W., Xing, Y., Zhang, H., Yang, S., Chen, L., Bartolomei, M. S.,.... You can therefore change the genomic target of the Cas enzyme by simply changing the target sequence...

... Monomer pET mOrange LIC cloning vector (u-mOrange) - Bacterial Expression pCS2+8CmOrange - Zebrafish/...urchin pCS2+8NmOrange - Zebrafish/Xenopus/Worm/Sea urchin mOrange-N1 - Mammalian Expression mOrange-C1 - Mammalian...Expression mOrange-pBAD - Bacterial Expression mOrange2 549 565 35 6.5 4.5 h Monomer mOrange2-N1 - Mammalian...Expression LSSmOrange 437 572 23 5.7 2.3 h Monomer pLSSmOrange-C1 - Mammalian Expression pLSSmOrange-N1 - Mammalian...Structure Plasmids mKusabira-Orange (mKO) 548 559 31 5 4.5 h Monomer pQC mKorange IX - Mammalian Expression...Expression mKusabira Orange-C1 (mKO) - Mammalian Expression mKO-pBAD - Bacterial Expression mOrange 548 562 49 ...Mammalian Expression mOrange2-C1 - Mammalian Expression mOrange2-pBAD - Bacterial Expression mKO2 551 565...

..., Gong, L., Yang, X., He, L., Zhang, L., Yang, Z., Geng, J. J., Chen, R., Zhang, H., Wang, B., Zhu, Y....Cui, H.Y., Huang, M., Zhang, J., Fu, L., Yang, X.M., Zhao, Z., Sun, S., Gu, H., Wang, Z., Wang, C.F., Lu... new window) Wang, K., Chen, W., Zhang, Z., Deng, Y., Lian, J. Q., Du, P., Wei, D., Zhang, Y., Sun, X.... M., Nan, G., Jiang, J.L., Li, L., Wu, J., Lin, P., Huang, W., Xie, L., Zhe, Z.H., Zhang, K., Miao, J....for SARS-CoV-2 and SARS-CoV is the angiotensin receptor, Angiotensin Converting Enzyme 2 (...protein and is involved in virus entry into cells (Wang et al., 2020). Cathepsin L plasmids - a cysteine...Lu, Y., Liu, Y.Y., Wang, Q.Y., Bian, H., Zhu, P., Chen, Z. N. (2020). CD147-spike protein is a novel route...

..., a conformational change in the sensing region of the construct leads to a change in signal of a circularly...Wolf Frommer Calcium Orange fluorescent calcium ion indicator OCaMP A sensitive orange fluorescent calcium...GECI) that reports with lifetime and intensity changes A turquoise fluorescence lifetime-based biosensor... biosensors from nested reporters of green- and orange-emitting proteins Ratiometric Matryoshka biosensors...biosensors from a nested cassette of green- and orange-emitting fluorescent proteins. Nat Commun. 2017 Sep...activities in live cells. eLife. 2018 Jul 3;7. Jin Zhang Calcium Yellow protein calcium sensor jYCaMP1 variants...Islets. Anal Chem. 2019 Oct 1;91(19):12212-12219. Huiwang Ai Zinc GZnP2 Cytosolic fluorescent sensor for ...

... CMV Alzheimer's Karsten Melcher 178115 ANG_Halo_C_allele ANG Halo ALS Michael Ward 178116 PRNP_Halo_C_allele...Dong-Er Zhang 12421 pMCSF-R(mA)-luc CSF1R CSF1R Hereditary diffuse leukoencephalopathy Dong-Er Zhang 12422...Dong-Er Zhang 12424 pMCSF-R(DD)-luc CSF1R CSF1R Hereditary diffuse leukoencephalopathy Dong-Er Zhang 12425...ATM H1 Ataxia telangiectasia Didier Trono 14581 pSUPER.ATMi ATM H1 Ataxia telangiectasia Didier Trono ...TARDBP GFP CMV ALS Zuoshang Xu 28196 tdp43-EGFP construct3 TARDBP GFP CMV ALS Zuoshang Xu 28197 tdp43-EGFP...TARDBP GFP CMV ALS Zuoshang Xu 28198 tdp43-EGFP construct5 TARDBP GFP CMV ALS Zuoshang Xu 28199 tdp43-EGFP...TARDBP GFP CMV ALS Zuoshang Xu 28200 tdp43-EGFP construct7 TARDBP GFP CMV ALS Zuoshang Xu 28201 tdp43-EGFP...

...Bacterial TAG Huiwang Ai 153558 pMAH-EcaYRS EcaYRS E. coli 3-aminotyrosine Mammalian Huiwang Ai 154762 pAS_FLAG-Mma...TAG sites changes to UAG, RF1 function removed George Church 48999 C321 all TAG sites changes to UAG George...sites changes to UAG, RF1 function removed Farren Isaacs 87359 C321.∆A.opt all TAG sites changes to UAG...protein of interest. Applications By making small changes in selected amino acids within a protein, any alterations...introduced amino acid can also be used to intentionally change the activity of a protein (e.g. converting a DNA...tyrosyl-tRNA synthetase E. coli pBof Mammalian TAG Huiwang Ai 68292 SepOTSλ SepRS9 E. coli Sep Bacterial TAG...)acetyl)lysine (DnpK) Bacterial, Mammalian TAG Huiwang Ai 71404 pMAH2-CageCys leucyl-tRNA-synthetase E...

...BA, Schnitzbauer J, Zhang W, Li GW, Park J, Blackburn EH, Weissman JS, Qi LS, Huang B. 2013. Dynamic imaging... Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F. 2013. Multiplex genome engineering... as guanosine, so the overall effect is an A->G change. Since this method targets RNA rather than DNA,...cerevisiae and S. pombe ) Zebrafish Xenopus References Barrangou R, Fremaux C, Deveau H, Richards M, Boyaval P,...27096365 Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T. 2015. Multicolor CRISPR labeling ...PMID: 25713381 Ma H, Tu LC, Naseri A, Huisman M, Zhang S, Grunwald D, Pederson T. 2016. Multiplexed labeling...Alkhnbashi OS, Costa F, Shah SA, Saunders SJ, Barrangou R, Brouns SJ, Charpentier E, Haft DH, Horvath ...

...MISR2, MISRII ANGPT1 angiopoietin 1 AGP1, AGPT, ANG1 ANGPT4 angiopoietin 4 AGP4, ANG-3, ANG4, MGC138181,...MGC138183 ANGPTL1 angiopoietin-like 1 ANG3, ANGPT3, ARP1, AngY, KIAA0351, UNQ162, dJ595C2.2 ANGPTL2 angiopoietin-like..., HARP, MGC8889 ANGPTL3 angiopoietin-like 3 ANGPT5 ANGPTL4 angiopoietin-like 4 ANGPTL2, ARP4, FIAF, HFARP...pp1158 ANGPTL5 angiopoietin-like 5 - ANGPTL6 angiopoietin-like 6 AGF, ARP5 ANGPTL7 angiopoietin-like 7... 1 guanine nucleotide exchange factor VAV VAV2 vav 2 guanine nucleotide exchange factor - VAV3 vav 3 guanine...TPAR1 CXCL13 chemokine (C-X-C motif) ligand 13 ANGIE, ANGIE2, BCA-1, BCA1, BLC, BLR1L, SCYB13 CXCL14 chemokine...

...pBAD , pLSSmOrange-mKate2 LSSmOrange* mScarlet3 437 0.45 592 104,000 0.75 5.8 4.3 pLSSmOrange-N1 , pmScarlet3...2.9 mVenus N1 , mKO2-N1 mOrange2 mCherry 549 0.60 610 72,000 0.22 6.1 4.4 mOrange2-N1 , mCherry2-N1 miRFP670...-N1 , pmiRFP720-N1 LSSmOrange* mKate2 437 0.45 633 62,500 0.40 5.6 3.7 pLSSmOrange-N1 , mKate1.31-pBAD...for BRET assays. References Bajar, B. T., Wang, E. S., Zhang, S., Lin, M. Z., & Chu, J. (2016). A Guide...to measure protein interactions or conformation changes. Plasmid...to report on protein binding or conformational changes. Organic dyes, fluorescent proteins, and other ...intermolecular or bimolecular FRET) or (2) conformational changes within a protein where the donor and acceptor are...

...collection of CRISPR plasmid kits. CRISPR Software Sanger Indel Analysis ICE (Inference of CRISPR Edits) ... at a given locus. To use the tool, you'll need Sanger sequencing reads from PCR amplicons that cover ...sequence and checks for off-target binding. Supports a range of Cas enzymes (Cas9 and Cas12) and organisms. Developed...gene inactivation, NHEJ, and HDR. Includes a wide range of reference genomes, including animals, plants,...and cytosine (CBE) base editors. Includes a wide range of reference genomes, including animals, plants,...for off-target binding. Currently supports a wide range of organims. Developed by the Michael Boutros Lab...window) A Google groups forum started by the Feng Zhang Lab . Protocols Lab(s) Description Plasmids in protocol...

....0 37184 pCAG-T7-TALEN(Sangamo)-Destination Pawel Pelczar pCAG-T7-TALEN(Sangamo)-Destination constructs.... 40131 pCAG-T7-TALEN(Sangamo)-FokI-KKR-Destination 40132 pCAG-T7-TALEN(Sangamo)-FokI-ELD-Destination ...Validated in zebrafish somatic cells. Keith Joung Zhang Lab TALE Toolbox PCR/Golden Gate cloning method....method. Optimized for human expression. Feng Zhang LIC TAL Effector Assembly Kit Assembly by ligation-independent...TALEN backbone, which were initially published by Sangamo BioSciences (N153AA, C63AA) and showed robust cleavage...activity in several later studies. pCAG-T7-TALEN(Sangamo)-Destination vectors are available with homodimeric...mutations in Bombyx mori and Drosophila melanogaster . Zhang Lab TALE Toolbox 49622 - 49647 24 plasmids Thoru...

...of long-range inhibitory neurons. Preprint (Link opens in a new window) Yu K, Ahrens S, Zhang X, Schiff...ZR, Chen WZ, Liu MZ, Chen XJ, Wan L, Zhang XY, Yuan L, Lin JK, Wang M, Zhou L, Xu XH, Sun YG. 2019. Tac1...Haynes TM, Hochbaum DR, Huang KW, Hyun M, Umadevi Venkataraju K, Straub C, Wang W, Robertson K, Osten P... Perry C, Diester I, Boyce FM, Bass CE, Neve R, Huang ZJ, Deisseroth K. 2014. Targeting cells with single...AC, Tepler S, Poulin JF, Ansorge M, Awatramani R, Kang UJ, Rayport S. 2018. Dopamine neuron glutamate cotransmission...Neurons in Barrel Cortex Reveals Local and Long-Range Circuit Motifs. Cell Rep. 28(13):3450-3461. PubMed...

...bioluminescence found in a variety of organisms, ranging from bacteria to aquatic animals to insects. Luciferase...photons. Its robust signal, sensitivity, wide dynamic range, and resistance to photobleaching have made luciferase...transfection or viral infection efficiency, to study changes in cell physiology, or as a reference level of ...luciferase is used to monitor viral transfection Feng Zhang 21471 pLenti PGK V5-LUC Neo (w623-2) Firefly PGK...EF1α-Gaussia luciferase, CMV-Cypridina luciferase). Feng Zhang Return to Top Reporter Constructs Already know what...brighter signal and emission in the near-infrared range, ideal for in vivo imaging. teLuc : Red-shifted ... on protein-protein interactions, drug-induced changes, and more. NanoBit (Link opens in a new window)...

... producing antibodies, can change the constant region, and therefore change the isotype of antibodies ...variable regions, shown in light blue and light orange, that bind to a specific antigen, triggering an...constant region, and is shown in dark blue and dark orange. The constant region is a stable part of the antibody... There are several alternatives to antibodies, ranging from antibody fragments (shown in Figure 2) to ...expensive than HRP, fluorophores are available in a range of colors activated by different wavelengths, allowing... assay run. The standard curve should contain a range inclusive of all expected protein concentrates in...to distinguish cell types. Multiplex assays can range from two antibodies to upwards of twenty. Laser ...

...mutations, base changes, and large insertions or deletions. For more information on the wide range of CRISPR...transcriptional activators that need to be within a given range of the transcription start site in order to function...Plasmid delivery is versatile thanks to the wide range of options for the Cas enzyme, promoters, and selection...interference, and some targeted edits can lead to changes in more than just the DNA sequence. If this is ...heterozygous edits High sensitivity (good for rare edits) Sanger sequencing Knockouts Prime and base edits Good ...interference Needs a good antibody References Cong, L., & Zhang, F. (2015). Genome engineering using CRISPR-CAS9...

... protein 35 ARHGEF2 Rho/Rac guanine nucleotide exchange factor 2 CCND CCND1 CCND2 CCND3 Cyclin D CDK CDK4...Phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2 PRKA PRKAA1 PRKAA2 PRKAB1 PRKAB2 PRKAG1... RAPGEF RAPGEF1 RAPGEF2 Rap guanine nucleotide exchange factor RAS KRAS MRAS NRAS Kirsten rat sarcoma ...protein SOS SOS1 SOS2 SOS Ras/Rho guanine nucleotide exchange factor SPRED SPRED1 SPRED2 SPRED3 Sprouty related...Tuberous sclerosis VAV1 Vav guanine nucleotide exchange factor 1 YAP1 Yes associated protein 1 Return ...

... Vika. These systems work by sequential strand exchange at a specific target site (called loxP for Cre...Cre, or FRT for Flp). The nature of the DNA rearrangement after recombination (inversion, deletion, or ...Strategies Depending on how the target sites are arranged, recombinases can be used for more than inversion...genetic control. Recombinase-mediated cassette exchange (RMCE): When matching but mutually incompatible...Zeller Use this dual recombinase-mediated cassette exchange (dRMCE) plasmid kit to re-engineer conditional...

...into cholinergic neurons. Nat Commun. 2013;4:2183. Zhang Fetal Lung Fibroblasts Cholinergic Neurons Lentiviral...into cholinergic neurons. Nat Commun. 2013;4:2183. Zhang Fibroblasts Oligodendrocyte Precursor Cells Lentiviral...ALS Patients. Cell Rep. 2016 Jan 5;14(1):115-28. Zhang Adult Dermal Fibroblasts Neurons Lentiviral Human... Cell. 2019 Jun 6. Buganim Glial Cells Retinal Ganglion Cells AAV/CRISPR Mouse Glia-to-Neuron Conversion...Disease in Mice. Cell. 2020 Apr 30;181(3):590-603.e16. Yang iPSCs Bipolar Neurons Lentiviral Human Rapid neurogenesis...