Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 52 results for: Erf

Clear keywords
Showing: 1 - 20 of 52 results

  2. Immunology Research Plasmids and Resources

    Type
    Collection
    ...IFNA10 interferon, alpha 10 MGC119878, MGC119879 IFNA13 interferon, alpha 13 - IFNA14 interferon, alpha...IFNA16 interferon, alpha 16 - IFNA17 interferon, alpha 17 IFNA, INFA, LEIF2C1 IFNA2 interferon, alpha ...MGC142200 IFNA5 interferon, alpha 5 INFA5 IFNA6 interferon, alpha 6 - IFNA7 interferon, alpha 7 IFNA-J IFNA8...

  3. Viral Production

    Type
    Collection
    ...protocols and performing rigorous quality control (QC). The specific QC experiments performed varies for ...of Pennsylvania Vector Core. Transfections are performed using the transfer plasmid, a plasmid encoding...and stored at -80 °C. Titer Titering is either performed by Addgene or by the University of Pennsylvania...Pennsylvania Vector Core. In general, titering is performed by the facility that produced the viral vector lot....Pennsylvania Vector Core is (as of April 2016) also performed by droplet digital PCR (ddPCR). Quality Control.... To learn which specific QC experiments were performed on your lot, please contact us . Full sequencing...the Viral Genome Next-generation sequencing is performed on viral genomes isolated from the final AAV preparation...

  4. Plan Your Experiment

    Type
    Collection
    ...Activation/interference Needs good primer design Western blot Knockouts Activation/interference Needs a ... nterspaced S hort P alindromic R epeats) is a powerful system that enables researchers to manipulate ...Selecting the genetic manipulation you plan to perform will help you narrow down which components you’...optimization can be challenging Prime Edit Repress or Interfere (CRISPRi) Reduce gene expression without permanently...effective than dCas9 alone for mammalian cell lines Interfere Activate (CRISPRa) Increase gene expression without...be effective, as the Cas9 bound to the DNA can interfere with transcription elongation. CRISPR Activation...between on-target and off-target activity. In a perfect world, your gRNA sequence would be an exact complement...

  5. CRISPR Plasmids - Repress Gene Expression

    Type
    Collection
    ... CRISPR Interfere CRISPR Plasmids: Interfere Mammalian Bacteria Plant Yeast Resources...knock down gene expression by interfering with transcription. This interference is most efficient when the...Overview of CRISPR interference. Browse, sort, or search the tables below for CRISPR interference plasmids. To... To learn more about CRISPR interference and other CRISPR topics, read our CRISPR Guide . Mammalian ID...

  6. Mammalian RNAi Tools

    Type
    Collection
    ...Plasmids for mammalian RNA interference (RNAi) experiments at Addgene; browse by constitutive lentiviral...Tools for RNAi Browse Plasmids Resources RNA interference (RNAi) is an RNA-mediated gene silencing mechanism...as shRNA (short hairpin RNA) or siRNA (short interfering RNA). Delivery can occur via transfection or ...below. Figure 1: Overview of shRNA-mediated RNA interference. Created with BioRender.com. shRNA Plasmids ... Tuning silence: conditional systems for RNA interference . Nature Methods, 3 (9), 682–688. PubMed (Link..., L. (2023). A comprehensive review of small interfering RNAs (siRNAs): mechanism, therapeutic targets...

  7. Arf GTPase Family

    Type
    Collection
    ... Plasmid Collections ARF Superfamily Plasmids for ARF Superfamily Research You may also like... ...members of the RAS superfamily, though now have been argued to form their own superfamily (Klinger, et al...signaling activity. Figure 1: Locations of ARF superfamily members. Figure from Szutl, et al., 2019. Browse... window) Sztul, E., Chen, P., Casanova, J. E., Cherfils, J., Dacks, J. B., Lambright, D. G., Lee, F. S...

  8. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ... High-performance GFP-based calcium indicators (Constitutive or Cre-dependent) High-performance calcium...:547-63. Sergio Grinstein pH Superfolder pHluorin pH sensor A superfolder variant of pH-sensitive pHluorin...chloride sensitivity, photostability and reduced ph interference demonstrates augmented transmembrane chloride...Citron/Citroff), mitochondrial or cytosolic High-Performance Intensiometric Direct- and Inverse-Response Genetically...ApplePy1) fluorescent biosensors for pyruvate High-performance genetically-encoded green and red fluorescent...Voltage Voltage Sensitive Fluorescent Protein (VSFP/Butterfly) Knopfel Lab VSFP Plasmids Thomas Knopfel Voltage... Near-infrared fluorescent voltage indicator nirButterfly Screening and Cellular Characterization of Genetically...

  9. Antibody Guide

    Type
    Collection
    ...signaling molecules, as some buffer components can interfere with the conjugate or conjugation process. Sodium...used as an antimicrobial agent. Other chemical interferences with conjugating reactions are possible. It ... antibody to specifically bind to a protein is powerful, but in order to utilize it in the lab, researchers...fluorophores. Horseradish peroxidase (HRP) HRP performs a chemiluminescent reaction in the presence of...activated and its output measured. This method can be performed using the indirect detection method via a conjugated...protein complexes for downstream analysis. They are powerful tools for understanding protein or protein interactions...above. Reverse the crosslink, isolate DNA, and perform downstream analysis. Special considerations One...

  10. CRISPR Guide

    Type
    Collection
    ...scalable genome editing technologies, making CRISPR perfect for genome-wide screens. This guide will provide.... Ideally, a gRNA targeting sequence will have perfect homology to the target DNA with no homology elsewhere...compatible with truncated gRNAs to increase specificity SuperFi-Cas9 — increases fidelity with reduced nuclease...for sequences > 10 kb! Currently, these systems perform best in bacterial species, but newer versions engineered...More robust transcriptional repression (CRISPR interference, or CRISPRi) or activation (CRISPRa) of downstream...chromosome. Browse Plasmids: Activate , Repress/Interfere Epigenetic Modifications Using CRISPR A variation...transcription of a target gene CRISPRi CRISPR Interference; using a dCas9 or dCas9-repressor with a gRNA...

  11. Antibody Production

    Type
    Collection
    ...not compatible for use in live cells and will interfere with conjugation. For applications such as these...recommended applications. The specific QC experiments performed varies for each antibody catalog item. Whenever...Whenever possible, the experiment is performed in parallel with a previous lot of recombinant antibody or the.... To learn which specific QC experiments were performed on your lot, please email [email protected] . Immunocytochemistry...

  12. AAV Packaged on Request

    Type
    Collection
    ...this time, we amplify DNA, produce the prep, and perform comprehensive quality control processes to make...whether the item can be packaged. Addgene does not perform functional or in vivo testing. We recommend that...chosen AAV plasmid for more information on its performance. ID Plasmid Gene/Insert Promoter PI Additional...

  13. Plant Plasmids and Resources

    Type
    Collection
    ...Resources Improved Gateway binary vectors — High-performance vectors for creation of fusion constructs in ...the expression of microRNAs (miRNAs) or small interfering RNAs (siRNAs) to achieve gene silencing in plants.../Cas9 CRISPR-mediated Plant Base Editors RNA Interference in Plant Biology: New Tools for an Old Favorite...

  14. CRISPR Plasmids - Plants

    Type
    Collection
    ...CRISPR Plasmids: Plants Activate Base Edit Cut Interfere Nick Prime Edit Resources The following CRISPR...Selectable Marker PI Publication Return to top Interfere ID Plasmid Gene/Insert Promoter Selectable Marker...

  15. CRISPR Plasmids - Yeast

    Type
    Collection
    ...CRISPR Plasmids: Yeast Activate Base Edit Cut Interfere Nick Purify Resources The following CRISPR plasmids...Selectable Marker PI Publication Return to top Interfere ID Plasmid Gene/Insert Promoter Selectable Marker...

  16. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...CRISPR Plasmids: Bacteria Activate Base Edit Cut Interfere Nick Prime Edit Purify RNA Targeting Resources.../Insert Promoter PI Publication Return to top Interfere ID Plasmid Gene/Insert Promoter PI Publication...

  17. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...Expression Activate Base Edit Cut Epigenetics Interfere Nick Prime Edit Purify RNA Editing RNA Knockdown...Selectable Marker PI Publication Return to top Interfere ID Plasmid Gene/Insert Promoter Selectable Marker...

  18. CRISPR Plasmids and Resources

    Type
    Collection
    ... activator peptide activates gene expression. Interfere dCas9 alone, or fused to a repressor peptide, ...Other Applications Screen CRISPR libraries are a powerful tool for genome-wide screening. Purify dCas9 fused...

  19. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...guarantee the function of any viral vector. Addgene performs QC measures which verify the identity of the viral...shipped, Addgene can provide the QC validation data performed on the material received. Shipping While Addgene...

  20. Genetic Code Expansion

    Type
    Collection
    ...Mammalian TAA Simon Elsaesser 157925 pMAH-POLY-eRF1(E55D) POLY-eRF1(E55D) E. coli para-substituted phenylalanine...182653 pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA Y306A/Y384F (AF) pyrrolysine (Pyl) tRNA...
Showing: 1 - 20 of 52 results