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We narrowed to 129 results for: SCR

Showing: 1 - 20 of 129 results

  1. CRISPR Guide

    Type
    Collection
    ...integration of donor DNA. Genome-Wide Screens Using CRISPR In a genetic screen, a population of cells with mutations...Targeting dCas9 to transcription start sites is sufficient to repress transcription by blocking initiation..., G. M. (2013). CAS9 transcriptional activators for target specificity screening and paired nickases for...in your lab, from knockouts to pooled library screening. Science...CRISPR Basics Small Edits Large Edits Genome-Wide Screening Cas9 Fusion Tools Cas9 Alternatives Resources ...approaches like zinc finger nucleases (ZFNs) or transcription-activator-like effector nucleases (TALENs) required...technologies, making CRISPR perfect for genome-wide screens. This guide will provide a basic understanding ...

  2. Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection

    Type
    Collection
    ...The collection can be used for screening transcription factors for cellular programming or cancer drug ...simplifies identification of the transcription factor isoform in pooled screens. This collection and library...library can be used for screening transcription factors for applications such as cellular programming or cancer...for NGS QC and bulk screening (PDF, 85 KB) TF ORF barcode dial out PCR for 10X scRNA-seq (PDF, 59.2 KB)...to simplify identification during screening. Library ID PI Description MORF library 137000, 192821, 1000000218...Browse the Zhang lab’s comprehensive human transcription factor collection, available at Addgene. The...Library The MORF library is a comprehensive human transcription factor collection that contains 1,836 genes ...

  3. Plan Your Experiment

    Type
    Collection
    ... interfere with transcription elongation. CRISPR Activation : Target the transcription start site. CRISPRa...fused to transcriptional activators that need to be within a given range of the transcription start site...validate your genetic edit. Figure 1: Flow chart describing the general framework of a CRISPR experiment....Prime editor (Cas9 nickase fused to reverse transcriptase (RT)) Can edit within a wider window than most...genome sequence dCas9 or dCas9 fusion with transcriptional repressor (such as dCas9-KRAB) dCas9-KRAB can...modifying the genome sequence dCas9 fusion with transcriptional activator (such as dCas9-VP64) Many different... interest, where the Cas9 protein can block transcription from ever initiating. gRNAs targeting regions...

  4. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...Access to the Paper & Protocol Video Download the manuscript here: Bauer et al, 2014 322.2 KB If you have ...production of genomic deletions. In addition, we describe techniques for deletion, identification, and characterization...referenced Tables and Figures, please access the manuscript PDF posted at the top of the page. CRISPR Design... the genomic recognition site. NOTE: Figure 1 describes possible deletion strategies for genes and non-coding...leading to nonsense mediated decay of the mRNA transcript ( Figure 1B ). Use the example guides for the...be 24-mer oligos ( Table 4 ). Design Deletion Screening Primers Design one set of primers internal to ...deletion. For this approach, the internal primers described in step 2.1 may be omitted. CRISPR Cloning Anneal...

  5. Luciferase Plasmid Collection

    Type
    Collection
    ...promoter/enhancer regions. Blue/white screening can be used for screening of transformants in cloning process...Optimized STARR-seq (Self-Transcribing Active Regulatory Region) : Genome-wide screening of enhancers using ... protein half-lives, making them useful as transcriptional reporters, but are less-than-ideal protein ...mammalian systems. ID Plasmid Luciferase Type(s) Description PI 64784 pGL3-Basic-IRES Firefly Insertion of...Vector for investigating regions controlling transcription Debrya Groskreutz 18760 MSCV IRES Luciferase...Vector for investigating regions controlling transcription Pete Stecha 212933 pGL4.82(hRluc/Puro) Renilla...Vector for investigating regions controlling transcription Pete Stecha 106292 pLS-SV40-mP-Rluc Renilla ...

  6. Zhang Lab CRISPR Page

    Type
    Collection
    ...below. Return to top Cas9 SAM transcriptional activation plasmids and screening library CRISPR/Cas9 Synergistic...RNA 48137 : PX165; SpCas9 Return to top GeCKO Screening Library GeCKO are pooled lentiviral CRISPR libraries...and mouse) for performing genome-wide knockout screens. The libraries are available in two formats: 1 ...) is an engineered protein complex for the transcriptional activation of endogenous genes. It consists...database (23,430 isoforms) for gain-of-function screening. For more information, please visit the Addgene... references are below. Available plasmids are described below: 61591 : PX601; CMV-driven SaCas9; U6-driven...sgRNA to a constitutively Cas9-expressing mouse. Described here are AAV vectors that can be combined with...

  7. Tetracycline Inducible Expression

    Type
    Collection
    ...Plasmid Description PI 63800 PB-TRE-dCas9-VPR Tet-inducible expression of SP-dCas9-VPR transcriptional activator..., the TetR protein binds to tet O, blocking transcription of the downstream gene. If tet or one of its...to tetracycline and releases tet O, enabling transcription (Figure 1). While TetR and tet O could be a ...tetracycline-controlled transactivator (fusion of TetR with VP16 transcriptional activation domain); rtTA: reverse tetracycline-controlled...transactivator (fusion of mutated TetR with VP16 transcriptional activation domain). For simplicity, other promoter...to eliminate binding of endogenous mammalian transcription factors to further minimize background expression...plasmids use "TetO" or "Tet-O" in their name or description, meaning they contain a TRE or tet O and can ...

  8. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...: dCas9 fused to transcriptional activators or repressors can modulate transcription in a reversible manner...palindromic repeat sequences. (1) The CRISPR array is transcribed to make the pre-CRISPR RNA (pre-crRNA). (2) The...in vitro. In 2013, Cong et al. and Mali et al. described CRISPR-based genome editing in mammalian cell ... of new applications. The first CRISPR papers described two main categories of genome edits. First, the... locus makes it especially amenable to genetic screens. Pooled gRNA libraries can be used to identify ...are available for gene knockout, as well as transcriptional activation or repression. Combined with the...sequencing, CRISPR is a robust system for genome-wide screening. CRISPR Technological Advances CRISPR technology...

  9. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...mutant cells that are then screened for a phenotype of interest. Screening experiments using a pooled ...CRISPR pooled libraries for large-scale functional screens. Available for knockout, activation, inhibition...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...Blog Posts CRISPR is a useful tool for genetic screening experiments, due to the relative ease of designing... gRNAs for each target gene. In a CRISPR screening experiment, target cells are treated with the ...libraries and keep the following in mind: CRISPR screening experiments require electroporation to amplify...

  10. Michael Davidson Fluorescent Protein Collection

    Type
    Collection
    ... Plasmid Description Industry mEmeralds Plasmid Description Industry Reds Plasmid Description Industry... Plasmid Description Industry Cyans Plasmid Description Industry Oranges Plasmid Description Industry ...Industry Greens Plasmid Description Industry Yellows Plasmid Description Industry Photoactivatables Plasmid Description... Description Industry Do you have suggestions for other plasmids that should be added to this list? Fill...

  11. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...pSerOTS-C1* (V70) 188537 ). These reagents are described in detail in (Mohler et al., Mol Syst Biol 2023...at the central position. Introducing Hi-P for screening phosphorylation-dependent protein-protein interactions...NEDD4-2; 111705-8 ) is available from Addgene. Screening kinases. The mode #1 phosphosite library can also...Phosphorylated Isomers (iSPI). This new resource described in (Gassaway et al., Nature Methods 2022) is adapted...Iterative Synthetically Phosphorylated Isomers (iSPI) described in: A multi-purpose, regenerable, proteome-scale...in a new window) rEcoli XpS and a new pSerOTS described in: System-wide optimization of an orthogonal ..., 551 KB) Human Serine Phosphopeptide Library described in: Encoding human serine phosphopeptides in bacteria...

  12. TALEN Plasmids and Kits

    Type
    Collection
    ... ID Plasmid Depositing PI Description Golden Gate TALEN 2.0 38142 RCIscript-GoldyTALEN Daniel Carlson ...new tools for genome engineering. Fusions of transcription activator-like (TAL) effectors of plant pathogenic...with custom repeat arrays. TALEN Kits TALEN Kit Description Depositing PI FusX TALEN Assembly System Contains...expression of TALENs from a truncated CAGs promoter. RCIscript-GoldyTALEN is designed for in vitro synthesis ...strong nuclear localisation tags and repress transcription initiation when targeted to DNA sequences within...destination vectors can be used to create TALE-transcription factors and are used at the final assembly step...TALEN kit, (ii) a lacZ fragment for blue/white-screening in E.coli , (iii) CAG promoter and Kozak sequence...

  13. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. ...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs... Edit Cas9 H840A nickase fused to a reverse transcriptase (RT) is capable of installing targeted insertions...-dU6:3gRNA dU6:3 BbsI Injection or in vitro transcription Virmilion none, need Cas9 plasmid Bullock and... dU6:1 and dU6:3 BbsI Injection or in vitro transcription Virmilion none, need Cas9 plasmid Bullock and...-dU6:1gRNA dU6:1 BbsI Injection or in vitro transcription Virmilion none, need Cas9 plasmid Bullock and...

  14. CRISPR References and Information

    Type
    Collection
    ...- a CRISPR Screen Tool (Link opens in a new window) Design gRNAs for custom CRISPR screens targeting a...in your lab, from knockouts to pooled library screening. CRISPR eBook Addgene's downloadable eBook about...CRISPR pooled libraries for large-scale functional screens. Available for knockout, activation, inhibition...For use with genome-scale CRISPR-Cas9 knockout screens (or GeCKO) technology. Developed by the Xiaole ...batch (Link opens in a new window) for lentiviral screens. sgRNA Scorer 2.0 (Link opens in a new window) ...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations,...started by the Feng Zhang Lab . Protocols Lab(s) Description Plasmids in protocol Download protocol Addgene...

  15. Caltech Systemic Capsids

    Type
    Collection
    ...at the bottom of this page. For more in depth descriptions of each serotype, check out Addgene's blog on...Browse Available PHP.eB AAV ID Name Promoter Description Category PI Controls 28306 pAAV-FLEX-tdTomato... Browse Available PHP.S AAV ID Name Promoter Description Category PI 28306 pAAV-FLEX-tdTomato CAG tdTomato...Browse Available PHP.V1 AAV ID Name Promoter Description Category PI 104052 pAAV-CAG-DIO-EYFP CAG EYFP...Browse Available MaCPNS1 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control...Browse Available MaCPNS2 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control...Browse Available CAP-B10 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control...

  16. CRISPR Plasmids - Yeast

    Type
    Collection
    ...dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. ...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...via homology-directed repair (HDR). ID Plasmid Description Gene/Insert Promoter Selectable Marker PI Publication...Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide like KRAB, can knock down...down gene expression by interfering with transcription. Design your gRNA to target your gene of interest...direct dCas9 to a specific locus, avoiding known transcription factor and other protein binding sites. ID Plasmid...

  17. COVID-19 Resources

    Type
    Collection
    ... Plasmids expressing Cas12a/Cas13a, Reverse Transcriptases, etc. that may be useful for detection assays... industry is continually growing. ID Plasmid Description Industry PI Return to top Other Coronavirus Plasmids... SARS-CoV, MERS, BAT-CoV Plasmids ID Plasmid Description Industry PI Due to export control regulations...plasmids related to these genes below. ID Plasmid Description Industry PI For more information on these genes...2020.06.23.166397 (Link opens in a new window) reverse transcriptase, protease, and Bst polymerase constructs now...unpublished) Taq polymerase and MMLV reverse transcriptase expression plasmids from the Drew Endy and Philippa...2020.03.29.013342 (Link opens in a new window) Reverse Transcriptase plasmids from Andrew Ellington's lab. High-surety...

  18. Plasmids for Stem Cell Research

    Type
    Collection
    ...find more information. Delivery Method Species Description Article PI Lentivirus Human Expression of human...human Klf4, Oct4, c-Myc, and Sox2 as VP-16 transcriptional activating fusions in single lentiviral vectors...human iPSC generation OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts...Progenitor Cells Lentiviral Human Cooperative Transcription Factor Induction Mediates Hemogenic Reprogramming...Oligodendrocyte Progenitor Cells Lentiviral Mouse Transcription factor-mediated reprogramming of fibroblasts...fibroblasts into functional astrocytes by defined transcription factors. Stem Cell Reports. 2015 Jan 13;4(1)...Neurons Lentiviral Human Chemical modulation of transcriptionally enriched signaling pathways to optimize the...

  19. Plant Plasmids and Resources

    Type
    Collection
    ...expression in plants: Maize Transcription Factor ORFeome - John Gray. Rice Transcription Factor ORFeome - John... Addgene Blog: Plant Biology CRISPR Cloning Transcription Factors RNAi Empty Backbones Fluorescent Proteins...plant transformation vectors - Daniel Voytas. Transcription Factors The following collections can be used...John Gray. Plant transcription factors for Agrobacterium-mediated transient expression or plant transformation...promoter or cloning method of choice. ID Plasmid Description Promoter Cloning Method PI Return to top Fluorescent...or fluorescent protein of choice. ID Plasmid Description Promoter PI Return to top Other Resources Addgene...Genomics Resource (Link opens in a new window) - Transcriptomic and metabolomic resources for 14 key medicinal...

  20. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. ...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs... Edit Cas9 H840A nickase fused to a reverse transcriptase (RT) is capable of installing targeted insertions...Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide like KRAB, can knock down...down gene expression by interfering with transcription. Design your gRNA to target your gene of interest...direct dCas9 to a specific locus, avoiding known transcription factor and other protein binding sites. ID Plasmid...
Showing: 1 - 20 of 129 results