We narrowed to 29 results for: Scrib

...Salvador family WW domain containing protein 1 SCRIB Scribbled planar cell polarity protein SHC SHC1 SHC2 ...

... (V70) (Plasmid #188537) . These reagents are described in detail in Mohler et al. (2023) and help deliver...Phosphorylated Isomers (iSPI) The iSPI resource described in Galloway et al. (2022) is adapted from the ...Iterative Synthetically Phosphorylated Isomers (iSPI) described in: Gassaway, B. M., Li, J., Rad, R., Mintseris...in a new window) rEcoli XpS and a new pSerOTS described in: Mohler, K., Moen, J. M., Rogulina, S., & Rinehart..., 551 KB) Human Serine Phosphopeptide Library described in: Barber, K. W., Muir, P., Szeligowski, R. V...in a new window) Improved Reagents (ca. 2015) described in: Pirman, N. L., Barber, K. W., Aerni, H. R....Reagents (DOCX, 525.9 KB) Reagents (ca. 2014) described in: Heinemann, I. U., Rovner, A. J., Aerni, H....

...expression plasmids. Large collections or kits are described below and not included in this table. For a more...strains. CRISPR plasmids from John Calarco's lab as described in Heritable genome editing in C. elegans via ...genome-editing events. Developed in Craig Mello's lab and described in A Co-CRISPR Strategy for Efficient Genome Editing...also been developed by the Fire laboratory and described in Efficient Marker-Free Recovery of Custom Genetic...C. elegans and other nematodes. Wormbuilder - Describes methods to engineer the genome of the genetic ...

...zebrafish (can be used as a substitute for pMLM335 described in Foley et al., PLoS One 2009) pMLM292 (for expression...zebrafish (can be used as a substitute for pMLM336 described in Foley et al., PLoS One 2009) pMLM800 (for expression...Methods 2011 ) in mammalian cells or zebrafish as described by Moore et al. PLoS One 2012 and Cade et al.,...

... references are below. Available plasmids are described below: 61591 : PX601; CMV-driven SaCas9; U6-driven...sgRNA to a constitutively Cas9-expressing mouse. Described here are AAV vectors that can be combined with...Constitutively active Cas9 mouse Available plasmids are described below: 61408 : Targeting vector for the mouse ... backbone plasmid for cloning new targets are described here. #60224 - AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA...present in the mouse genome. These plasmids are described here. #60227 - AAV:ITR-U6-sgRNA(NeuN)-pCBh-Cre-WPRE-hGHpA-ITR...

...validate your genetic edit. Figure 1: Flow chart describing the general framework of a CRISPR experiment.... mRNA and Proteins In addition to the methods described above, you can also directly deliver mature mRNA...cells. In this case, your target cells are not transcribing DNA to mRNA, nor do they need to translate mRNA...directly combine purified Cas protein and in vitro transcribed gRNA to form a Cas-gRNA complex. You then delivery...endosomal approach. Similar to the mRNA method described above, this method is also transient, as the RNPs...

...that came out in the spring of 2011, the groups describe a set of customized plasmids that can be used ... a TALEN kit through Addgene. This kit, first described in Nature Biotechnology in August, uses a serial...in Nature Biotechnology early in 2011 and they described their kit for assembling TAL effector arrays in...

... is described in more detail in the Multiplex Assays section below. The following table describes the ...of B cells isolated from animals immunized as described above are used to create hybridomas, which produce...primary antibody for your protein of interest, as described in the IP section above. Reverse the crosslink...

...palindromic repeat sequences. (1) The CRISPR array is transcribed to make the pre-CRISPR RNA (pre-crRNA). (2) The...in vitro. In 2013, Cong et al. and Mali et al. described CRISPR-based genome editing in mammalian cell ... of new applications. The first CRISPR papers described two main categories of genome edits. First, the...

...label of their choice according to the methods described by George et al. (2004). The ACP and SFP Synthases...respectively) may be expressed and purified as described previously in Stachelhaus et al. (1998) References...

...and educational use. Links to additional pages describing these reagents are provided below: Zinc Finger...

... Labs containing wild-type and mutant alleles described in the initial Target Accelerator publications...

...for FLASH assembly. Links to additional pages describing these reagents are provided below: Joung Lab ...

...using the bacterial two-hybrid system originally described by Hochschild and colleagues ( Dove et al., Nature...

...vector is taken up by a host, the viral genome is transcribed, producing the shRNA. Dicer, an RNase III enzyme...

... that we will not meet the turnaround time we described here or by email, Addgene will contact you to ...

...Voigt Clifford Wang Ron Weiss Synthetic biology describes the rational engineering of biological systems...

...protocols, titering methods, and quality control are described below. Information for a specific antibody can...

...expression plasmids. Large collections or kits are described below . ID Plasmid Description Gene/Insert PI ...

...protocols, titering methods, and quality control are described below. AAV Production AAV distributed by Addgene...