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  1. Zebrafish Plasmid Collection

    Type
    Collection
    ...been established as a powerful vertebrate model organism for the study of genetics and developmental biology...Multicolor fluorescent labeling in zebrafish Whole organism lineage tracing by combinatorial and cumulative... previously resistant cell types. CreLite - George Eisenhoffer Lab. An optogenetically controlled ...screening fluorescent protein fusion expression in organisms or cells in which an exogenous mRNA can be injected...assembly for the zebrafish genome. Zebrafish Model Organism Database (ZFIN) - A curated database of genetic... International Zebrafish Society (IZFS) - An organization that promotes and advocates for zebrafish research...aims to increase the use of zebrafish as a model organism and to foster advanced training of young scientists...

  2. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Scaffold for inorganic phosphate biosensor Development of a Reagentless Biosensor for Inorganic Phosphate...biosensors can be introduced into cells, tissues, or organisms for long-term imaging and can be designed to specifically...specifically localize to cellular compartments or organelles. Addgene’s database includes many fluorescent.... in vivo , cytoplasmic vs. extracellular vs. organelle-localized). Browse or search the tables below ...and In Vivo Imaging of Ca Dynamics in High-Ca Organelles. Cell Chem Biol. 2016 Jun 1. pii: S2451-9456(...sensors (green, red or blue/green) Imaging intraorganellar Ca(2+) at subcellular resolution using CEPIA...Genetically Encoded FRET Sensor for Cytosolic and Intraorganelle Zn(2+) Imaging. ACS Chem Biol. 2015 Sep 18;...

  3. Worm Expression Resources

    Type
    Collection
    ... “the worm”) is widely used as a genetic model organism due to their rapid life cycle (3 days from egg...Lab website . SapTrap CRISPR/Cas Toolkit - Erik Jorgensen Lab. A collection of plasmids that streamlines... Transgenesis miniMos Transgenesis Kit - Erik Jorgensen Lab. This kit contains 29 plasmids used to generate... related miniMos plasmids can be found on the Jorgensen Lab page. More information on miniMos and MosSCI...methods to engineer the genome of the genetic model organism C. elegans. Return to top Do you have suggestions...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  4. Genetic Code Expansion

    Type
    Collection
    ...RF1 function removed George Church 48999 C321 all TAG sites changes to UAG George Church 49018 C321.ΔA.exp...pairs of synthetase and tRNA that work for one organism may not work for another. The orthogonal synthetase...removed, MutS restored so decreased mutation rate George Church 68306 C321.ΔA all TAG sites changes to UAG..., RF1 function removed, improved doubling time. George Church 98564 C321.Ub-UAG-sfGFP all TAG sites changes...function removed, with Ubiquitin-UAG-sfGFP reporter George Church 98565 C321.ΔClpS.Ub-UAG-sfGFP all TAG sites... inactivated, with Ubiquitin-UAG-sfGFP reporter George Church 174513 Syn61 No TCG, TCA, or TAG codons ...codons. Deletion of serT, serU, prfA, and recA genes George Church 192872 rEcoli XpS All 321 UAG codons changed...

  5. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ... structures give the cell its shape, organize cellular organelles, and are necessary for movement and ... Plasmid Collections Fluorescent Proteins Organelle Markers Fluorescent Proteins: Subcellular Localization... fusion proteins that are targeted to various organelles or subcellular structures. You should fuse your...General Structures The cell contains a variety of organelles and subcellular structures each with their own...table below to find markers of general cellular organelles and structures. ID Plasmid Structure Targeting...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  6. Microbiology Resources

    Type
    Collection
    ...these model organisms. See below for plasmid kits and other resources relevant to these organisms, or use ...depositors are making this possible in a range of organisms. Find CRISPR tools for use in genome editing of...extensive fluorescent protein plasmid collection is organized by application and by color. External Resources...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  7. Plan Your Experiment

    Type
    Collection
    ...these principles apply to using CRISPR in other organisms. First, consider the genetic manipulation that...carry out your experiment in your particular model organism. There may not be a perfect plasmid for your specific...disrupt gene function in a particular cell type or organism without a specific preferred mutation Cas9 (or...sequence and design your gRNA. Know your cell line/organism and genomic sequence When possible, you should...may vary depending on the specific cell line or organism, which may affect the observed efficiency of CRISPR...

  8. Structural Genomics Consortium Plasmids

    Type
    Collection
    ...Related Resources SGC: http://www.thesgc.org/ SGC Toronto: https://www.thesgc.org/scientists/groups/toronto...Structural Genomics Consortium is a not-for-profit organization that aims to determine the three dimensional...www.sgc.ox.ac.uk/ Protein Database (PDB): http://www.pdb.org Request Plasmids To order, Login or Register ...

  9. Distribution to Industry

    Type
    Collection
    ...account and affiliate it with your organization. If your organization has not ordered from Addgene in the...fax, or email. Pricing Addgene is a nonprofit organization dedicated to providing the scientific community...through your user account or by email to mta@addgene.org . After we review and approve the MTA documents...

  10. AAVED

    Type
    Collection
    ...MA, 02139 Organizing Committee Chair: Dr. Melina Fan, Co-Founder and CSO, Addgene Organizing Committee...2018 Contact Us Questions? Contact us at AAVED@addgene.org Sponsor We are grateful to The Kavli Foundation... your permission). Materials don't have to be organized (yet), we will take care of that. Note that you...

  11. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ... Fluorescent Protein Fusions (Organized by Expression Species) Organism/Expression System Plasmids Mammalian...proteins with your gene of interest. These are organized By Color of the fluorescent protein and By Expression...Empty Backbones for Fluorescent Protein Fusions (Organized by Color) For each table: Excitation and Emission...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource! Jump to Top...

  12. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...promoter that will be functional in your host organism. Host Relevant Promoters Representative Empty ...Selectable Marker Typical Host Organism Representative Empty Backbones Neomycin... elegans Transgenic Expression in organisms pBigT - For making transgenic mice...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  13. CRISPR Plasmids - Tagging

    Type
    Collection
    ...locus Jorgensen Lab SapTrap CRISPR/Cas Toolkit SapTrap is a modular toolkit from Erik Jorgensen's lab for... and a prebuilt Unc-32::GFP targeting vector. Jorgensen Lab SapTrap CRISPR/Cas Toolkit Kanemaki Lab Auxin-Inducible...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  14. Luciferase Plasmid Collection

    Type
    Collection
    ... bioluminescence found in a diverse number of organisms, ranging from bacteria to aquatic animals to insects...enhancer regions using Gateway cloning. Lentiviral. Jorge Ferrer 87067 pcDNA3.1-ccdB-Nanoluc NanoLuc® Creation... CMV Mammalian expression of firefly luciferase Georg Weber 113450 pLenti NL NanoLuc® Ubc Lentiviral expression...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  15. MAPK Plasmids

    Type
    Collection
    ...email deposit@addgene.org to be paired with an Addgene scientist. MAPK Plasmids (organized by gene) Gene...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  16. Synthetic Biology - Overview

    Type
    Collection
    ...Matthew Bennett Carolyn Bertozzi Nicolas Buchler George Church Cynthia Collins James Collins Tom Ellis ...plasmid-strain functional pairs. Browse Plasmids by Model Organism Bacterial Plant Yeast Mammalian Worm Fungal Algal...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  17. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...their choice according to the methods described by George et al. (2004). The ACP and SFP Synthases (AcpS ...previously in Stachelhaus et al. (1998) References George N, Pick H, Vogel H, Johnsson N, Johnsson K. 2004...Chem. 273(35):22773-81. PMID:9712910 Vivero-Pol L, George N, Krumm H, Johnsson K, Johnsson N. 2005. Multicolor...

  18. KLF Research Plasmids

    Type
    Collection
    ...Depositing Labs Juan Belmonte Linzhao Cheng Jose Cibelli George Daley Jin-Tang Dong Steven Dowdy Scott Friedman...depositing plasmids with Addgene or email deposit@addgene.org to get started. Genes KLF gene pages with information...

  19. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...upregulating gene expression in various model organisms....out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  20. Cancer Research Plasmids and Resources

    Type
    Collection
    ...curated collection of plasmids for cancer research, organized by signaling pathway. ...constructs. Each collection page listed below organizes the plasmids and resources by signaling pathway...
Showing: 1 - 20 of 112 results