We narrowed to 93 results for: CAL

... site into a genomic attP site. So far, their practical application has been limited by low integration...sequential strand exchange at a specific target site (called loxP for Cre, or FRT for Flp). The nature of the...other recombinase systems. Enzyme Target Site Canonical Sequence Structure (Length) Common Variants Cre...repeats + 4 bp spacer (32 bp) Currently, only the canonical rox site is widely used. Flp FRT GAAGTTCCTATTCCGAAGTTCCTATTCtctagaaaGTATAGGAACTTC...Expression: A stop codon flanked with loxP sites (often called a "lox-STOP-lox" or "LSL" cassette) placed upstream...Placing the loxP sites on either side of a gene (called "floxing", for "flanked by loxP"), will permit ...excise the shRNA to return gene expression to physiological levels. In lox-STOP-lox shRNA constructs, Cre...

... of stochastic combinations of cell-filling antigenically distinct protein tags (or "bits"), creating ...well as barcoding with specific enhancers for morphological readout. How to Use PRISM Barcodes are delivered...AAVs either directly to a region of interest or systemically using a capsid capable of crossing the blood-brain...microscopy workflows to achieve high-resolution optical imaging down to individual synapses. Figure 2: ...new window) , and The Max Planck Institute for Biological Intelligence (Link opens in a new window) . Read...barcodes enable self-correcting neuron tracing with nanoscale molecular context . bioRxiv 2025.09.26.678648;...

..., excitatory and inhibitory opsins , genetically-encoded calcium indicators, and rabies targeting genes...doubly-specified combination of genetic and/or anatomical-defined parameters, by placing two orthogonal...fragments (which have become exons) determines the logical expression requirements of Cre and Flp (B,E). When...Fenno LE, Ramakrishnan C, Kim YS, Deisseroth K, Callaway EM, Oberhuber M, Conzelmann KK, Staiger JF. 2019...Parvalbumin-Expressing GABAergic Neurons in Barrel Cortex Reveals Local and Long-Range Circuit Motifs. Cell Rep. 28(13):...Robertson K, Osten P, Sabatini BL. 2019. Distinct Cortical-Thalamic-Striatal Circuits through the Parafascicular...

...’s disease (PD) is a chronic and progressive neurological disorder that primarily affects movement. More...-wide association studies (GWAS) or other large scale studies. Researchers are now investigating the role...3xFlag-eGFP-Flag-SETX construct for subcellular localization studies with GFP, or IP with Flag tag. (Bennett...organelles. (Mattia et al. EMBO Rep. 2018) Study intrinsically disordered protein regions with FUS and HNRNPA1...fluorescent proteins, including Markers of Subcellular Localization , Optogenetics Plasmids , and Chemogenetics ...(Link opens in a new window) A collection of neurological mouse models including complex and monogenic...

...with most experiments, you first need to have a biological question in mind. Then you can decide how to ...additional sites throughout the genome. These sites are called off-targets. Be aware of off-target sites when ...protein and gRNA, which you generally perform by chemical transfection or electroporation. Expression can...questions: Does the resulting population need to be identical (clonal) or is a mixed population okay? Does the...from a single cell and will result in cells with identical genomes. If your end goal is to establish an edited...Perturb-Seq: Dissecting Molecular Circuits with Scalable Single-Cell RNA Profiling of Pooled Genetic Screens... Kampmann, M., & Weissman, J. S. (2014). Genome-Scale CRISPR-Mediated control of gene repression and activation...

..., which allows you to visualize the cellular localization of your protein. Additionally, tags are...expression Fluorescent proteins (GFP, mCherry, etc) Localization pcDNA3-EGFP - C-terminal GFP for mammalian expression...the Michael Davidson FP Collection NLS Nuclear localization dCas9 plasmid - dCas9 expression plasmid without...pEntry vector that adds a C-terminal nuclear localization signal pGADCg - Gateway yeast expression vector...vector that adds a SV40 NLS Myr Membrane localization pWZL-Neo-Myr-Flag-DEST - pDest vector... the plasmid. Selectable Marker Typical Host Organism Representative Empty ...

...monitoring endogenous protein dynamics in highly scalable assays. HaloTag Fusions HaloTag (Link opens in...HaloTags form a covalent bond with these synthetic chemical ligands, which have been developed for special...levels in lysates or live-cells, thus preserving physiological relevance. NanoBRET Assays NanoBRET (Link opens...similar premise, but examine the proximity of a chemical compound and its target protein. The apparent ...Connections (Link opens in a new window) blogs for technical tips and tools for genetic reporter assays (Link...

...choice of seven serotypes and four volumes. Details Scale and Titer Packaged on Request can be ordered in ...preparations, we set both minimum standards and typical titers, though actual yields may vary by construct...construct. Size Quantity Total Volume Minimum Titer Typical Titer Small NEW 2 × 100 µL aliquots 0.2 mL 4 × ... with a minimum titer of 2 × 10 12 GC/mL and a typical titer of 7 × 10 12 GC/mL. For more details, see...providing scientist through an MTA. This process typically takes 1–4 days, depending on your institution....

...following regions: Isocortex Hippocampal formation Cortical subplate Striatum Pallidum Hypothalamus Cerebellum...pAAV-AiE2051m-minBG-SYFP2-WPRE3-BGHpA AiP1277 AiE2051m SYFP2 Claustrum Cortical subplate 220711 pAAV-AiE2051m-minBG-iCre(R297T...R297T)-BGHpA AiP1786 AiE2051m Cre Claustrum Cortical subplate 230518 pAAV-AiE2051m-minBG-FlpO-WPRE-HGHpA ...pAAV-AiE2051m-minBG-FlpO-WPRE-HGHpA AiP1211 AiE2051m FlpO Claustrum Cortical subplate 191720 pAAV-AiE0743m_3xC2-minBG-SYFP2-WPRE3-BGHpA ...and transgenic mouse lines for genetic access to cortical cell types. Cell , 188 (11), 3045–3064. https:...for Brain Science BRAIN Armamentarium Collection Caltech Systemic Capsids AAV Resources AAV Packaged on ...

...horizontal stripes on the sides of the adult body, is a tropical freshwater fish in the minnow family. It has long...inflammation and infection, regeneration, human neurological disorders, microbiota studies, and drug discovery...Cell lineage tracing in multicellular systems typically relies on genetic markers, somatic mutations, ...types. CreLite - George Eisenhoffer Lab. An optogenetically controlled Cre/loxP system that enables precise...assessment of lipoprotein abundance, size, and localization in high-throughput. MAZERATI platform - Leonard...

...expression, protein localization, protein–protein interactions, and other physiological processes in plants...repository contains many plasmid tools that were specifically designed for use in plants. These resources ...Infrastructure Kit Modules connect MoClo and Gateway for hierarchical assembly of plant multigene constructs; contains...an Old Favorite External Resources Arabidopsis Biological Resource Center (ABRC) (Link opens in a new window...

...to mentors, virtual and local professional development, membership to a local hub of educators teaching...Hypotheses in a Malate Dehydrogenase Oriented CURE. Callahan KP et al. CourseSource. 2022 Jan 14. DOI: 10.24918...Learning Gains and Positive STEM Attitudes in CUREs. Callahan KP et al. CBE Life Sci Educ. 2022 Dec;21(4):ar74...

...monoclonal antibodies with high specificity for critical axon guidance signaling proteins and growth cone...high-confidence targeting of proteins that have historically been difficult to identify and interrogate with...formation, neural connectivity, plasticity and neurological disorders. The initial collection includes antibodies...common epitope tags to help purify, detect, or localize particular proteins. Browse or search the table...

...vectors are popular for making stable cell lines or clinical use. Adenoviral Vector Guide | Adenoviral Plasmids...induce a strong immunogenic response, useful for clinical applications as vaccines. Selecting a Viral Vector... research goals. For example, AAV vectors are typically preferred for in vivo studies because of their...viral genome. Therefore, viruses used in research typically do not have the ability to propagate in the same...

...powerful tools to control gene expression and study biological targets. The tetracycline (tet) system is one...improvements. A synthetic Tet Response Element (TRE) typically features seven copies of tet O upstream of a minimal...or dox-dependent promoters (sometimes generally called pTet) have been designed by combining tet O sequences... to a reverse Tet repressor, or rTetR, which specifically binds the TRE in the presence of tetracycline...safe harbor site, for iPSC differentiation into cortical neurons Michael Ward 100798 pAAV-syn-FLEX-splitTVA-EGFP-tTA...

...deposited plasmids include: Joan Brugge , Harvard Medical School Klaus Hahn , UNC Richard Hynes , MIT Rick...University of Virginia Denise Montell , University of California, Santa Barbara Martin Schwartz , Yale University...University of Illinois Ron Vale , University of California, San Francisco Browse Plasmids ID Plasmid Gene...

...bacteria as model systems to answer many interesting biological questions. Escherichia coli is the most widely...enterokinase, thrombin Signal peptides for periplasmic localization: PelB, MalE, OmpA ID Plasmid Promoter Tags PI...purification tag combinations, including PelB tags for localization to the periplasm. The pCri System (Addgene #1000000058...protein of interest fused to FPs and monitor their localization in live cells. ID Plasmid Tags PI Additional...

...epigenetic modifications, researchers have fused catalytically dead Cas9 (dCas9) to epigenetic modifiers. Common...and H3K9 methylation Epigenetic modification is typically targeted to a specific promoter or enhancer for...interference technologies also indirectly modify local chromatin state. Figure 1: Overview of epigenetic...

...small biomolecules or other physiological intracellular processes with genetically encoded fluorescent biosensors...biosensors. Subcellular Localization Visualize organelles and subcellular structures with well-characterized...

...Archaerhodopsins Recombinases Cre, Flp, Dre Biosensors Calcium Sensors, Glutamate Sensors, Voltage Reporters Controls...retrograde Retrograde AAV To target projection neurons Caltech Systemic Capsids PHP.eB, PHP.S, PHP.V1, MaCPNS1...AAV2, AAV5, AAV6, AAV8, AAV9, AAV11, AAVrg, the Caltech systemic serotypes, or others, it will be listed...