We narrowed to 101 results for: Eras

...addition to individual plasmids, Addgene carries several useful toolkits and plasmid collections for use...includes plasmids containing promoters that drive general and species-specific gene expression. Cloning and...Infrastructure Kit Modules connect MoClo and Gateway for hierarchical assembly of plant multigene constructs; contains...selection and multiplex CRISPR/Cas9 assembly for fast generation of multiple Arabidopsis mutants. Engineered biocontainable...expression, protein localization, protein–protein interactions, and other physiological processes in plants...Plasmid Description PI Kits for Protein–Protein Interactions The following plasmid kits contain a variety...reporter plasmids for the study of protein–protein interactions in plants. ID Kit Description Cloning Type PI...

...highly-sensitive genetically encoded temperature indicator exploiting a temperature-responsive elastin-like polypeptide...Aug 13;11(1):16519. Takeharu Nagai Temperature Fluorescent temperature indicator B-gTEMP Intracellular Heat...family Cameleon calcium sensors Bcl-2-mediated alterations in endoplasmic reticulum Ca 2+ analyzed with ... cAMP levels for imaging in live cells Fourth-generation epac-based FRET sensors for cAMP feature exceptional...GMP) Green fluorescent probe for cGMP (cGull) Generation of a cGMP Indicator with an Expanded Dynamic ...Monitoring the action of redox-directed cancer therapeutics using a human peroxiredoxin-2-based probe. Nat...Yulong Li Dopamine Dopamine sensors GRAB_DA2 Next-generation GRAB sensors for monitoring dopaminergic activity...

...developing embryos. zfRegeneration - A database for gene expression profiling during regeneration. Seurat - R ...(embryogenesis is complete in ~3 days), short generation time (~3 months), ease of care and breeding, ...study of cancer, inflammation and infection, regeneration, human neurological disorders, microbiota studies...CRISPR Please refer to Addgene's CRISPR Guide for a general introduction to CRISPR technology or the zebrafish... Vivo Lineage Tracing during Development and Regeneration - Periklis Pantazis Lab Multibow: digital spectral...abundance, size, and localization in high-throughput. MAZERATI platform - Leonard Zon Lab. A platform to rapidly...Zebrafish Tol2 Gateway-compatible Kit for Neurodegenerative Disorders - Nicholas Cole Lab. A kit consisting...

...suitable for generating SARS-CoV-2 spike pseudotyped virus. It also lists several luciferase...Plasmids Other Coronavirus Plasmids Mammalian Targets General Plasmid Tools Pooled Libraries Other Resources ...Addgene blog Scientists Map the SARS-CoV-2-Human Interaction Network Antibody Neutralization Response Against...top Plasmids Encoding Mammalian Genes or Inserts Several mammalian genes have been identified as having ... our Immunology Resource Guide . Return to top General Plasmid Tools Browse the table below to find plasmid...reporters Reverse transcriptase, protease, and polymerase constructs for SARS-CoV-2 detection assays Neutralizing... host cells . Signal transduction and targeted therapy, 5 (1), 283. https://doi.org/10.1038/s41392-020...

...translation machinery are required: The ncAA is generally supplied in the culture media. A codon must be...achieve these modifications, two plasmids are generally used. Once these plasmids have been introduced...in selected amino acids within a protein, any alterations in structure or function in the protein can be...and evolve proteins for various purposes from therapeutics to biopolymers. Tips for Success Before beginning...beginning to reprogram the genetic code, there are several things to consider. If you are working off of a...tRNA pair instead. For directed evolution of the liberated EcTrpRS/tRNA pair to incorporate other Uaas (derived...

...estrogen receptor 1 DKFZp686N23123, ER, ESR, ESRA, Era, NR3A1 ESR2 estrogen receptor 2 (ER beta) ER-BETA...MGC3886 ERAP1 endoplasmic reticulum aminopeptidase 1 A-LAP, ALAP, APPILS, ARTS-1, ARTS1, ERAAP, ERAAP1, KIAA0525...disulfide isomerase family A, member 2 PDA2, PDI, PDIP, PDIR PDIA3 protein disulfide isomerase family A...KGFLP1 keratinocyte growth factor-like protein 1 MGC125746, MGC125747, MGC126891 KGFLP2 keratinocyte growth... TDGF1 teratocarcinoma-derived growth factor 1 CR, CRGF, CRIPTO, Cripto-1 TDGF3 teratocarcinoma-derived...systems. Upon infection, the immune system triggers several processes to activate cells to recognize and get...the dominant defense strategy during the first several days following exposure to a pathogen. The innate...

...accessory plasmids available from Addgene for generating your own specific TALE plasmids.... TALENs assembled by Golden Gate. Validated in several species. Takashi Yamamoto Additional Plasmids for... Gate TALEN kit (Bogdanove/Voytas) in order to generate TALORs (TAL Orthongal Repressors) that can be ...Grunwald pCS2TAL3-DD and pCS2TALE3-RR are next generation TALEN backbone vectors designed for use with ...segments (136AA and 63AA, respectfully). This next generation architecture has been shown to increase mutation... C63AA) and showed robust cleavage activity in several later studies. pCAG-T7-TALEN(Sangamo)-Destination.... In addition, a T7 promoter was included for generating TALEN mRNAs through in vitro transcription as...

... regulator controlling cell growth and proliferation. mTOR, a serine/threonine kinase, is found ...mutations in negative regulators of mTORC1. The hyperactivation of mTORC1 in cancer promotes the conditions...conditions needed for uncontrolled cell growth and proliferation, including an abundant supply of macromolecules...energy, as well as the presence of both pro-proliferative and anti-apoptotic signals. mTORC2, which appears...upstream of Akt, also promotes cell survival and proliferation. mTOR Pathway Plasmids Click on a name to find...specific relationship. The content for this page was generated with the help of David Sabatini . mTOR Pathway...specific relationship. The content for this page was generated with the help of David Sabatini . Return to top...

...Institute for Cell Science has deposited plasmids to generate fluorescently tagged proteins of key cellular ... stem cell (hiPSC) lines. The plasmids used to generate these lines have been deposited here with Addgene...With these plasmids researchers will be able to generate fluorescently tagged proteins of key cellular ...Microtubules 101786 ST6GAL1-mEGFP AICSDP-26 mEGFP Sialyltransferase 1 Golgi 109122 NPM1-mEGFP AICSDP-50 mEGFP ...164500 POLR2A-mEGFP AICSDP-117 mEGFP RPB1 RNA polymerase II 168798 TERF2-mEGFP AICSDP-77 mEGFP Telomeric...CTCF-binding sites 170818 PCNA-mEGFP AICSDP-116 mEGFP Proliferating cell nuclear antigen (PCNA) DNA replication ...Protocols The Allen Institute for Cell Science has several protocol videos available that may be helpful for...

... activator-like (TAL) effectors, scientists in several biological disciplines are coming closer to this...fingers recognize triplets and the FokI nuclease operates as a dimer, cutting in the spacer region between...triplet has a corresponding zinc finger, and interactions between zinc fingers within an array can reduce...zinc fingers, potential targetable sites in your average genome are every 500 bp. TAL effectors have some...history. Dr. Keith Joung’s lab at Massachusetts General Hospital also recently released a TALEN kit through... Zinc Finger Consortium with Dr. Voytas, has considerable experience in the DNA targeting field. A third...specific gene or your anxiously investigating gene therapy options for a rare disease in humans, TAL effectors...

...to LacZ, plus luciferase-2A-Cre recombinase 60226 : sgRNA cloning backbone with luciferase-2A-Cre recombinase...For cloning information, please view: Zhang Lab General Cloning Protocol 237.2 KB SpCas9 Plasmids The CRISPR...sgRNAs targeting KRAS , p53 , and LKB1 , plus luciferase-2A-Cre recombinase, and Kras G12D HDR template...of a single AAV vector ( AAV-KPL ) in the lung generated loss-of-function mutations in p53 and Lkb1 , as...plasmid contains two expression cassettes, Renilla luciferase-2A-Cre recombinase and sgRNAs targeting the mouse...plasmid contains two expression cassettes, Renilla luciferase-2A-Cre recombinase and an sgRNA targeted to LacZ...plasmid contains two expression cassettes, Renilla luciferase-2A-Cre recombinase and an sgRNA backbone for ...

...Resources Overview of Viral Production Viruses are generated using standard methods that have been optimized...optimized for each specific virus in order to generate high quality preparations. After production, all virus...the University of Pennsylvania Vector Core. In general, titering is performed by the facility that produced...suggests that titers obtained using ddPCR are generally 2–3 fold higher than those achieved using the ...us . Full sequencing of the Viral Genome Next-generation sequencing is performed on viral genomes isolated...preparations are produced in 293T cells using the 2nd generation psPAX2 and pMD2.G packaging system developed ...dilutions of the lentiviral vector, harvesting cells several days later, and isolating genomic DNA. This DNA...

...Class I PIN1 Peptidylprolyl cis/trans isomerase, NIMA-interacting 1 PLXNB1 Plexin B1 PPP1CA Protein phosphatase...relationship. The content and map for this page were generated with the help of Dominic Esposito and the Frederick... related tyrosine kinase 3 FNT FNTA FNTB Farnesyltransferase, CAAX box FOS FBJ murine osteosarcoma viral...protein 2 ICMT Isoprenylcysteine carboxyl methyltransferase INSR Insulin receptor INSRR Insulin receptor-related... . Targeting RAS signalling pathways in cancer therapy. Downward J. Nat Rev Cancer. 2003 Jan;3(1):11-22...

... genomic engineering Luciferase Reporter pGL3 Basic Vector - Firefly luciferase vector for investigating... to create a luciferase reporter Promoter Measure promoter strength pBV-Luc - Luciferase ...investigating regions controlling transcription See our Luciferase collection of plasmid backbones into which you...target genes can be inserted into this firefly luciferase reporter to test for their effects on protein...from an hU6 and an mU6 promoter Protein-protein interaction Proximity-dependent biotin identification (BioID...

...Expression mEmerald 487 509 39 6 11 min Monomer (A206K) mEmerald-N1 - Mammalian Expression mEmerald-C1 - Mammalian...Expression mPA-Emerald 487 509 400 Monomer (A206K) mPA-Emerald-N1 - Mammalian Expression mPA-Emerald-C1 - Mammalian... Mammalian Expression mEmerald-pBAD - Bacterial Expression EGFP 488 507 34 6 25 min Prone to dimerization...Michael Davidson Fluorescent Protein Collection Luciferase Plasmid Collection Promega Plasmid Collection...

...transduction of the trabecular meshwork following intracameral injection in mice, rats, and dogs. This serotype...serotype displays highly efficient transduction and lateral spread in the retina following subretinal injection...E531D) serotype has increased transduction and lateral spread in the retina following subretinal injection...Transduction optimization of AAV vectors for human gene therapy of glaucoma and their reversed cell entry characteristics...Display Exceptional Characteristics for Retinal Gene Therapy. Mol Ther . Jun 3;28(6):1464-1478. PMID: 32304666...

...resources for FRET experiments to measure protein interactions or conformation changes....FRET is often used to study (1) protein-protein interactions where each protein is separately fused to a ...proximity, affinity, and stoichiometry of protein interactions using image-based quantitative two-hybrid FRET...biosensors in Addgene's Biosensors Collection . Generate custom FRET-based biosensors with the cpFRET kit...Read more about self-labeling tags on our blog . Luciferase can be paired with a fluorescent protein acceptor...Resonance Energy Transfer (BRET) . Browse Addgene's Luciferase Plasmid Collection or the Promega Plasmid Collection...

...and throughput over traditional tagging methods. Several different tagging techniques, as well as the plasmids...plasmids include Cre and FLP expression vectors, a general cloning plasmid, and a prebuilt Unc-32::GFP targeting...Auxin-inducible degron (AID) tagging with CRISPR/Cas to generate conditional alleles of essential nuclear and cytoplasmic...technique for use in Drosophila cells that uses PCR to generate both an expression cassette for the Cas9-programming...cells 3.3 MB Seydoux C. elegans Tagging System Geraldine Seydoux's lab has developed a systematic and scalable...elegans . This system uses a 10-day protocol, generates “clean” homozygous mutants with no co-integrated...

...produced in-house by Addgene scientists. They are generated using standard methods individually optimized ...optimized for each specific antibody in order to generate high quality preparations. Following production, all...using ImageJ software and a standard curve is generated. The final antibody concentration is calculated...labeled with the antibody and a species-specific horseradish peroxidase (HRP)-conjugated secondary antibody...passed stability testing, it may ship at room temperature. However, Addgene determines ideal shipping conditions...

...their cellular machinery to generate viral vectors for research and gene therapy. Created with BioRender....can direct long-term transgene expression, but generally don't integrate into the host genome. AAVs are...Gamma-retroviral Stable RNA < 8 kb 80–130 nm Dividing Yes Moderate AAV Transient or Stable* Single-stranded linear...in the same way that wild-type viruses do. For general virus biosafety guidelines, check out Addgene's...