...lentiviral and adenoviral transfer vectors with Addgene. Lentiviral Transfer Vectors Within this set of...packaging these transfer vectors and generating infectious virus. Protocol: Lentivirus Transfer Vector Production...Plasmid FAQs Biosafety Resource Guide Lentiviral Transfer Vectors Adenoviral Vectors Kazuhiro Oka and the... of lentiviral transfer vectors you can find reporter plasmids to test whether or not you've efficiently...coexpressed with tdTomato and Pac (puromycin N-acethyl-transferase) pCDH-CB-copGFP-T2A-iCre 72256 Expresses copGFP...PGK promoter Adenoviral Vectors The adenoviral transfer vectors contained within this set are derived ...Flp recombinases. Genes can be cloned into these transfer vectors using shuttle vectors pICPIS-EF1 and pICPIS-CB...

...Desired Genetic Manipulation Different genetic manipulations require different CRISPR components. Selecting...be present in a single lentiviral transfer vector or separate transfer vectors May contain reporter gene...experimental goal, you are ready to start navigating the different reagents that are available for your particular...particular cell type or organism without a specific preferred mutation Cas9 (or Cas9 nickase) Single (or dual...can make a limited set of mutations. Repress or Interfere (CRISPRi) Reduce expression of a particular gene...targeting promoter elements of target gene Many different activators exist, including the multi-plasmid ...transduction CRISPR elements are inserted into an AAV transfer vector and used to generate AAV particles (for...

...Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing...vector, but their function (cut, nick, activate, interfere, etc.) is limited to that of the Cas protein present...have plasmids with Cas9 that can cut, activate, interfere, or nick. Selection , such as Puromycin or EGFP...pyogenes EGFP Ebert pLKO.1-puro U6 sgRNA BfuAI stuffer 50920 Mammalian/Lentiviral BfuAI none S. pyogenes...Joung pCRISPathBrick 65006 Bacteria BsaI yes, interfere S. pyogenes Koffas BPK764 65767 Bacteria BsaI ...pyogenes Joung MSP712 65768 Bacteria BsaI yes, interfere S. pyogenes Joung sgRNA with U6 promoter 48962...pyogenes Bar Chen pBUN6I11 50580 Plant BsaI yes, interfere S. pyogenes Bar Chen pBUN411 50581 Plant BsaI ...

...Kasparov Popular γ-Retroviral Transfer Plasmids Common γ-retroviral transfer plasmids. Want a retroviral...18760 MSCV IRES Luciferase MSCV Plasmid for transgene expression; also expresses luciferase Lowe 9044 pMIG...Addgene, including popular packaging, envelope, and transfer plasmids. Viral...scientists discuss retrovirus, they typically are referring to a subset of retroviruses called γ-retroviruses...of interest? Search our database for retroviral transfer plasmids that have inserts. ID Plasmid 5'LTR Description...Sun 60683 pLXIN-Luc MoMSV Stable expression of luciferase in mammalian cells Wong Return to Top Do you ...

...formulated in one of the following three buffers. All buffers are supplemented with 0.001% pluronic F68... or 0.014% Tween 20. Phosphate Buffered Saline (PBS) A universal buffer suitable for any application Balanced...as blood, vitreous, CSF, etc. Not suitable as a buffer for vectors delivered directly to the brain parenchyma...Shannon Boye’s lab at the University of Florida. References for each serotype can be found below under Citations...is available in each of the above serotypes and buffers. Please click on the plasmid name for ordering ...re Looking For? Our Packaged on Request service offers you even more options. Select from a vast catalog...

...cortical neuron differentiation. Held et al. Cell Rep. 2023 Aug 30. Enhance differentiation to mesencephalic...Collection CRISPR Tools AAV Viral Preps iPSC Differentiation Factors Antibodies Fluorescent Proteins Other...Amyotrophic lateral sclerosis (ALS), sometimes referred to as Lou Gehrig’s disease, is a progressive disease...are prone to misfolding and aggregation and can interfere with protein-protein interactions and nerve cell...of these are due to a mutation in one of three different inherited genes: Presenilin 1, Presenilin 2, and...al. Nat Methods. 2023 May 4. See More iPSC Differentiation Factors Find plasmids used to transform iPSCs...progression of Huntington’s disease. The foundation offers curated information on tools and reagents (Link...

...expressing firefly luciferase. pCMV-FLuc - Retroviral reporter vector expressing firefly luciferase. pHAGE-CMV-Luc2... vector expressing firefly luciferase and ZsGreen. NL4-3 mCherry Luciferase - Lentiviral dual reporter...EGFP. Lenti-luciferase-P2A-Neo - Lentiviral reporter vector expressing firefly luciferase with neomycin...reporter vector expressing mCherry and firefly luciferase. pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro...

... mutations in methyl-CpG-binding protein 2 confer different severity in Rett syndrome. Neurology . 70,...system for generating Rett syndrome-specific differentiated cells that can be used to understand the mechanisms...immortalized pre-neuronal cell line that can be differentiated into neurons Coriell Institute: Patient-specific... editing of mutations in the MECP2 gene. Please refer to Addgene's CRISPR Guide for a general introduction...microarray data, ISH image data, and anatomic reference atlases of prenatal and adult human brain (Link...(Link opens in a new window) Rettsyndrome.org References Amir et al. 1999. Rett syndrome is caused by ...MeCP2 levels display tissue- and cell-specific differences and correlate with neuronal maturation. Hum Mol...

...resonance energy transfer, or FRET , is a process by which energy is non-radiatively transferred from an excited...excited donor fluorophore to an acceptor. Since the transfer of energy does not occur by emission of a photon...FRET experiments. The efficiency of the energy transfer is measured using fluorescence microscopy by exciting...separately fused to a donor or acceptor molecule (also referred to as intermolecular or bimolecular FRET) or (...acceptor are both fused to the same protein (also referred to as intramolecular or unimolecular FRET). Fluorescent...fused between ECFP and Venus Back to Top FRET Reference Standards The following plasmids have well-characterized...

...of the applicable transfer plasmid. We will help you gain legal usage of the transfer plasmid from the ...handled at the lowest biosafety protection levels. We offer 959 in-stock viral vector preps, delivering high...service lets scientists choose from over 3,000 transfer plasmids to be packaged in their choice of five...chemogenetic, optogenetic, recombinase, and biosensor transfer plasmids in our repository are eligible for this...several important steps: MTA facilitation for the transfer plasmid, including legal approval and an implementation...

...Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing...on a gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end ...Insert Promoter Selectable Marker PI Publication Interfere Catalytically dead dCas9, or dCas9 fused to a ...like KRAB, can knock down gene expression by interfering with transcription. Design your gRNA to target...pyogenes Bar Chen 50580 pBUN6I11 OsU3 BsaI yes, interfere S. pyogenes Bar Chen 50582 pBUN501 AtU6-26 BsaI...pyogenes Hyg Chen 50587 pHSN6I01 AtU6-26 BsaI yes, interfere S. pyogenes Hyg Chen 50589 pHSN501 AtU6-26 BsaI...

...GSG-P2A-GCaMP6f-WPRE-pA Syn GCaMP6f mRuby2 Constitutive 1 Bonhoeffer , Huebener , Rose 51083 AAV-EF1a-DIO-GCaMP6f-...GSG-P2A-GCaMP6f-WPRE-pA CAG GCaMP6f mRuby2 Cre dependent 1 Bonhoeffer , Huebener , Rose 83899 pAAV-mDlx-GCaMP6f-Fishell...GSG-P2A-GCaMP6s-WPRE-pA Syn GCaMP6s mRuby2 Constitutive 1 Bonhoeffer , Huebener , Rose 51082 AAV-EF1a-DIO-GCaMP6s-...GSG-P2A-GCaMP6s-WPRE-pA CAG GCaMP6s mRuby2 Cre dependent 1 Bonhoeffer , Huebener , Rose 68720 pAAV-hSyn1-Flex-mRuby2...GSG-P2A-GCaMP6s-WPRE-pA Syn GCaMP6s mRuby2 Cre dependent 1 Bonhoeffer , Huebener , Rose 100842 pAAV.CAG.Flex.GCaMP6s.WPRE.SV40... AAV retro helper plasmid from the Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . ...re Looking For? Our Packaged on Request service offers you even more options. Select from a vast catalog...

... it is the actual material being referenced in the Material Transfer Agreement (MTA) and its inclusion...options below to get started. In-Stock Preps We offer commonly used AAV and lentiviral vectors as in-stock...in-stock preps? Our Packaged on Request service offers you even more options. Select from a vast catalog... vectors from the Penn Vector Core have been transferred to Addgene for distribution. Look up the IDs ...

...reasons: Transfer plasmids with sequence from the 5′ to the 3′ ITR exceeding 4.7 kb Transfer plasmids ... Terms of Purchase and the applicable material transfer agreements (MTAs) and limited use label licenses...guaranteed here or by email, Addgene will contact you to offer some options for moving forward with a helpful solution...described here or by email, Addgene will contact you to offer some options for moving forward with a helpful solution...

...Addgene AAV transfer plasmids...viral genome (typically called cis -plasmid or transfer plasmid). Helper Plasmid ID Plasmid Serotype Description...plasmid, expressing Rep2 and 7M8 capsid Flannery , Schaffer 64867 shh10 shh10 AAV packaging plasmid, expressing...expressing Rep2 and engineered shh10 capsid Flannery , Schaffer 65213 pSR651 AAV1 Baculovirus-mediated rAAV production...Olig001 encodes a novel AAV capsid that exhibits preferential oligodendrocyte tropism in the CNS McCown 183749...variant effective at targeting subventricular NSCs Schaffer 213968 AAV8R-12 Capsid AAV8R12 AAV packaging vector...

...Plasmids Supplemental Information New England Biolabs offers non-toxic protein labeling systems based on SNAP...constructs are compatible with multiple applications (different fluorophores, biotin, bead purification, blocking.... For a comprehensive comparison to GFP, please refer to NEB's comparison of SNAP-tag, CLIP-tag, and GFP... is based on the human O6-alkylguanine-DNA-alkyltransferase (hAGT), a DNA repair protein, and is suitable...described previously in Stachelhaus et al. (1998) References George N, Pick H, Vogel H, Johnsson N, Johnsson...

... Material Transfer Agreements Most Addgene orders must be accompanied by a material transfer agreement...agreement (MTA), including transfers to for-profit entities. The MTA is a legal agreement between the recipient... the depositing institution(s). It governs the transfer of biological materials and intellectual property...Please see the Sample Agreements on our Technology Transfer Information page for more information. To learn...

... regulator controlling cell growth and proliferation. mTOR, a serine/threonine kinase, is found ...conditions needed for uncontrolled cell growth and proliferation, including an abundant supply of macromolecules...energy, as well as the presence of both pro-proliferative and anti-apoptotic signals. mTORC2, which appears...upstream of Akt, also promotes cell survival and proliferation. mTOR Pathway Plasmids Click on a name to find...Screening Kit (from David Sabatini & Kris Wood) References mTOR signaling in growth control and disease....

...Constitutive 5, 8 Wilson 105532 pAAV.CMV.ffLuciferase.SV40 CMV ffLuciferase Constitutive 8 Wilson 105536 ...These AAV can be used to compare the activity of different serotypes. Serotype AAV1 AAV2 AAV5 AAV8 AAV9 AAVrh10... AAV retro helper plasmid from the Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . ...re Looking For? Our Packaged on Request service offers you even more options. Select from a vast catalog...

...migration, survival, adhesion, growth, and differentiation. Schematic of Ras signaling cascade that starts...ultimately turn on genes involved in cell growth, differentiation and survival. Ras is essential for regulating... related tyrosine kinase 3 FNT FNTA FNTB Farnesyltransferase, CAAX box FOS FBJ murine osteosarcoma viral...protein 2 ICMT Isoprenylcysteine carboxyl methyltransferase INSR Insulin receptor INSRR Insulin receptor-related...Initiative - at the National Cancer Institute (NCI) References Ras oncogenes and their downstream targets. Rajalingam...