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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 119 results for: FER

Showing: 21 - 40 of 119 results

  1. Retrovirus Plasmids

    Type
    Collection
    ...Kasparov Popular γ-Retroviral Transfer Plasmids Common γ-retroviral transfer plasmids. Want a retroviral...18760 MSCV IRES Luciferase MSCV Plasmid for transgene expression; also expresses luciferase Lowe 9044 pMIG...Addgene, including popular packaging, envelope, and transfer plasmids. Viral...scientists discuss retrovirus, they typically are referring to a subset of retroviruses called γ-retroviruses...of interest? Search our database for retroviral transfer plasmids that have inserts. ID Plasmid 5'LTR Description...Sun 60683 pLXIN-Luc MoMSV Stable expression of luciferase in mammalian cells Wong Return to Top Do you ...

  2. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...formulated in one of the following three buffers. All buffers are supplemented with 0.001% pluronic F68... or 0.014% Tween 20. Phosphate Buffered Saline (PBS) A universal buffer suitable for any application Balanced...as blood, vitreous, CSF, etc. Not suitable as a buffer for vectors delivered directly to the brain parenchyma...Shannon Boye’s lab at the University of Florida. References for each serotype can be found below under Citations...is available in each of the above serotypes and buffers. Please click on the plasmid name for ordering ...re Looking For? Our Packaged on Request service offers you even more options. Select from a vast catalog...

  3. Neurodegeneration Research Collection

    Type
    Collection
    ...cortical neuron differentiation. Held et al. Cell Rep. 2023 Aug 30. Enhance differentiation to mesencephalic...Collection CRISPR Tools AAV Viral Preps iPSC Differentiation Factors Antibodies Fluorescent Proteins Other...Amyotrophic lateral sclerosis (ALS), sometimes referred to as Lou Gehrig’s disease, is a progressive disease...are prone to misfolding and aggregation and can interfere with protein-protein interactions and nerve cell...of these are due to a mutation in one of three different inherited genes: Presenilin 1, Presenilin 2, and...al. Nat Methods. 2023 May 4. See More iPSC Differentiation Factors Find plasmids used to transform iPSCs...progression of Huntington’s disease. The foundation offers curated information on tools and reagents (Link...

  4. Tetracycline Inducible Expression

    Type
    Collection
    ...transcriptional and proliferative state of each cell in a population. Proliferation history is monitored...express both components in the same relative ratio. Different generations of transactivators and promoters are...new window) for comparison of Tet-On systems in different applications. Use of Tetracycline or a Derivative...Doxycycline, a derivative of tetracycline, is a preferred effector for tet-responsive systems. Dox binds...pSBtet-GP Sleeping Beauty transposon system with luciferase in cloning site. See article ( Kowarz et al.,...172115 PB-TO-hNGN2 Piggybac Tet-On plasmid for differentiating iPSCs into glutamatergic neurons via human ...NGN2 into AAVS1 safe harbor site, for iPSC differentiation into cortical neurons Michael Ward 100798 pAAV-syn-FLEX-splitTVA-EGFP-tTA...

  5. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...expressing firefly luciferase. pCMV-FLuc - Retroviral reporter vector expressing firefly luciferase. pHAGE-CMV-Luc2... vector expressing firefly luciferase and ZsGreen. NL4-3 mCherry Luciferase - Lentiviral dual reporter...EGFP. Lenti-luciferase-P2A-Neo - Lentiviral reporter vector expressing firefly luciferase with neomycin...reporter vector expressing mCherry and firefly luciferase. pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro...

  6. Viral Vector Guides and Plasmids

    Type
    Collection
    ...more about the different types of viral vectors and find viral production and transfer plasmids for a ...Gamma-retroviral Plasmids Gamma-retroviral vectors, often referred to as just retroviral vectors, have a relatively...Viral Vector Type Each viral vector type exhibits different properties and are suited for specific research...goals. For example, AAV vectors are typically preferred for in vivo studies because of their low immunogenicity...containing the genetic information to be delivered (transfer or shuttle plasmid) and one or more plasmids containing...Specific modifications to these plasmids, such as different promoters or tags, can even direct delivery of...

  7. Rett Syndrome

    Type
    Collection
    ... mutations in methyl-CpG-binding protein 2 confer different severity in Rett syndrome. Neurology . 70,...system for generating Rett syndrome-specific differentiated cells that can be used to understand the mechanisms...immortalized pre-neuronal cell line that can be differentiated into neurons Coriell Institute: Patient-specific... editing of mutations in the MECP2 gene. Please refer to Addgene's CRISPR Guide for a general introduction...microarray data, ISH image data, and anatomic reference atlases of prenatal and adult human brain (Link...(Link opens in a new window) Rettsyndrome.org References Amir et al. 1999. Rett syndrome is caused by ...MeCP2 levels display tissue- and cell-specific differences and correlate with neuronal maturation. Hum Mol...

  8. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...resonance energy transfer, or FRET , is a process by which energy is non-radiatively transferred from an excited...excited donor fluorophore to an acceptor. Since the transfer of energy does not occur by emission of a photon...FRET experiments. The efficiency of the energy transfer is measured using fluorescence microscopy by exciting...separately fused to a donor or acceptor molecule (also referred to as intermolecular or bimolecular FRET) or (...acceptor are both fused to the same protein (also referred to as intramolecular or unimolecular FRET). Fluorescent...fused between ECFP and Venus Back to Top FRET Reference Standards The following plasmids have well-characterized...

  9. Bacterial Expression Systems

    Type
    Collection
    ...transcription factors. Addgene Blog Luciferase Technologies Enabled by NanoLuc® Luciferase Fluorescent Biosensors ...replication in many gram-negative bacteria with different purification tag combinations, including PelB ... Protein Interactions Förster resonance energy transfer (FRET) and bimolecular fluorescence complementation...that when a donor fluorophore is excited, it can transfer its energy to an acceptor fluorophore if they ...of small molecules, light, and temperature, in different bacterial systems. ID Plasmid Promoter Inducer...easily measurable reporter genes (e.g., LacZ, luciferase, or fluorescent proteins) under the control of...FFluc Promoter activity Luminescence (firefly luciferase) Mycobacterium sp. Brian Robertson , Siouxsie...

  10. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...TALEN array for genomic engineering Luciferase Reporter See our Luciferase collection of plasmid backbones... to create a luciferase reporter Promoter Measure promoter strength pBV-Luc - Luciferase ...an insert using viral vectors, are studying a different genetic element, or want to express shRNA. Choose...target genes can be inserted into this firefly luciferase reporter to test for their effects on protein...lentiCRISPRv2 hygro - Variant of lentiCRISPR v2 that confers hygromycin resistance pRGEB32 - Express sgRNA/PTG...

  11. AAV Packaged on Request

    Type
    Collection
    ...of the applicable transfer plasmid. We will help you gain legal usage of the transfer plasmid from the ...handled at the lowest biosafety protection levels. We offer 1,001 in-stock viral vector preps, delivering high...service lets scientists choose from over 4,000 transfer plasmids to be packaged in their choice of seven...chemogenetic, optogenetic, recombinase, and biosensor transfer plasmids in our repository are eligible for this...several important steps: MTA facilitation for the transfer plasmid, including legal approval and an implementation...

  12. CRISPR Plasmids - Plants

    Type
    Collection
    ...Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing...on a gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end ...Insert Promoter Selectable Marker PI Publication Interfere Catalytically dead dCas9, or dCas9 fused to a ...like KRAB, can knock down gene expression by interfering with transcription. Design your gRNA to target...pyogenes Bar Chen 50580 pBUN6I11 OsU3 BsaI yes, interfere S. pyogenes Bar Chen 50582 pBUN501 AtU6-26 BsaI...pyogenes Hyg Chen 50587 pHSN6I01 AtU6-26 BsaI yes, interfere S. pyogenes Hyg Chen 50589 pHSN501 AtU6-26 BsaI...

  13. Biosensor AAV Preps

    Type
    Collection
    ...GSG-P2A-GCaMP6f-WPRE-pA Syn GCaMP6f mRuby2 Constitutive 1 Bonhoeffer , Huebener , Rose 51083 AAV-EF1a-DIO-GCaMP6f-...GSG-P2A-GCaMP6f-WPRE-pA CAG GCaMP6f mRuby2 Cre dependent 1 Bonhoeffer , Huebener , Rose 83899 pAAV-mDlx-GCaMP6f-Fishell...GSG-P2A-GCaMP6s-WPRE-pA Syn GCaMP6s mRuby2 Constitutive 1 Bonhoeffer , Huebener , Rose 51082 AAV-EF1a-DIO-GCaMP6s-...GSG-P2A-GCaMP6s-WPRE-pA CAG GCaMP6s mRuby2 Cre dependent 1 Bonhoeffer , Huebener , Rose 68720 pAAV-hSyn1-Flex-mRuby2...GSG-P2A-GCaMP6s-WPRE-pA Syn GCaMP6s mRuby2 Cre dependent 1 Bonhoeffer , Huebener , Rose 100842 pAAV.CAG.Flex.GCaMP6s.WPRE.SV40... AAV retro helper plasmid from the Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . ...re Looking For? Our Packaged on Request service offers you even more options. Select from a vast catalog...

  14. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...enzyme is present. Read on to learn about the different ways that Cre-lox and other site-specific recombinases...C-terminal fragments and placed under the control of different promoters. Expression of both fragments in the...the recombinase to align with your organism's preferred codon usage will improve expression. For example...of the orientation specificity of Cre and the different types of target sites available, both mutant and...such as Drosophila and mouse, particularly in proliferating cells. Expression may cause repetitive recombination...Use this AAV CRISPR knockout library expressing luciferase and Cre to interrogate tumorigenesis in vivo ...

  15. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ... it is the actual material being referenced in the Material Transfer Agreement (MTA) and its inclusion...options below to get started. In-Stock Preps We offer commonly used AAV and lentiviral vectors as in-stock...in-stock preps? Our Packaged on Request service offers you even more options. Select from a vast catalog... vectors from the Penn Vector Core have been transferred to Addgene for distribution. Look up the IDs ...

  16. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...reasons: Transfer plasmids with sequence from the 5′ to the 3′ ITR exceeding 4.7 kb Transfer plasmids ... Terms of Purchase and the applicable material transfer agreements (MTAs) and limited use label licenses...guaranteed here or by email, Addgene will contact you to offer some options for moving forward with a helpful solution...described here or by email, Addgene will contact you to offer some options for moving forward with a helpful solution...

  17. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...Plasmids Supplemental Information New England Biolabs offers non-toxic protein labeling systems based on SNAP...constructs are compatible with multiple applications (different fluorophores, biotin, bead purification, blocking.... For a comprehensive comparison to GFP, please refer to NEB's comparison of SNAP-tag, CLIP-tag, and GFP... is based on the human O6-alkylguanine-DNA-alkyltransferase (hAGT), a DNA repair protein, and is suitable...described previously in Stachelhaus et al. (1998) References George N, Pick H, Vogel H, Johnsson N, Johnsson...

  18. mTOR Pathway

    Type
    Collection
    ... regulator controlling cell growth and proliferation. mTOR, a serine/threonine kinase, is found ...conditions needed for uncontrolled cell growth and proliferation, including an abundant supply of macromolecules...energy, as well as the presence of both pro-proliferative and anti-apoptotic signals. mTORC2, which appears...upstream of Akt, also promotes cell survival and proliferation. mTOR Pathway Plasmids Click on a name to find...Screening Kit (from David Sabatini & Kris Wood) References mTOR signaling in growth control and disease....

  19. Distribution to Industry

    Type
    Collection
    ... Material Transfer Agreements Most Addgene orders must be accompanied by a material transfer agreement...agreement (MTA), including transfers to for-profit entities. The MTA is a legal agreement between the recipient... the depositing institution(s). It governs the transfer of biological materials and intellectual property...Please see the Sample Agreements on our Technology Transfer Information page for more information. To learn...

  20. Control AAV Preps

    Type
    Collection
    ...Constitutive 5, 8 Wilson 105532 pAAV.CMV.ffLuciferase.SV40 CMV ffLuciferase Constitutive 8 Wilson 105534 ...These AAV can be used to compare the activity of different serotypes. Serotype AAV1 AAV2 AAV5 AAV6 AAV8 AAV9... AAV retro helper plasmid from the Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . ...re Looking For? Our Packaged on Request service offers you even more options. Select from a vast catalog...
Showing: 21 - 40 of 119 results