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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 88 results for: Kin

Showing: 21 - 40 of 88 results

  1. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ... does not require a specific flanking sequence on the target RNA, making it a very flexible system. Other...rather than DNA, sometimes requiring a protospacer flanking sequence (PFS). In bacteria, Cas13 targeting also...):442-51. PMID: 23849981 Ishino Y, Shinagawa H, Makino K, Amemura M, Nakata A. 1987. Nucleotide sequence...

  2. Plasmids for Stem Cell Research

    Type
    Collection
    ... vector for "hit and run" reprogramming of adult skin fibroblasts to induced pluripotent stem cells. Stem...non-integrating episomes to allow sorting and dosage tracking of reprogramming factors Fluorescent tagged episomals...Stem Cell. 2012 Apr 6;10(4):465-72. Schöler Adult Skin Fibroblasts Cholinergic Neurons Lentiviral Human...Cell Stem Cell. 2015 Nov 5;17(5):543-56. Buganim Skin Fibroblasts Motor Neurons Lentiviral Human Direct...

  3. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...pAAV-AiE2150m-minBG-SYFP2-WPRE3-BGHpA AiP1379 AiE2150m SYFP2 Purkinje cells Cerebellum 208141 pAAV-AiE0390m_3xC2-minBG-SYFP2...Ariza J, Dee N, Reding M, Ronellenfitch K, Mufti S, Sunkin SM, Smith KA, Esposito L, Waters J, Thyagarajan...Smith K, Way SW, Esposito L, Yao Z, Gradinaru V, Sunkin SM, Lein E, Levi BP, Ting JT, Zeng H, Tasic B. ... C, Ko AL, Ojemann JG, Keene CD, Silbergeld DL, Sunkin SM, Gradinaru V, Horwitz GD, Zeng H, Tasic B, Lein...

  4. Plan Your Experiment

    Type
    Collection
    ...setting up CRISPR experiments. We will focus on making single edits using CRISPR/Cas9 in mammalian cells...experiments and can be used as positive controls when working out a new protocol. Read more about how to design... the cell type. Before proceeding, we recommend asking labmates/colleagues, searching the literature, ...efficiency. Edit Type The type of edit you are looking for will ultimately depend on which CRISPR method...

  5. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ...finger nucleases (ZFNs) are effective tools for making targeted mutations in zebrafish. With support ...OZ526 OPEN OPEN tCCCATCCTAtcgttGCATGAGCTc mdka (midkine-related growth factor) OZ527 and OZ528 OPEN OPEN...engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays. Maeder ML et al....

  6. Distribution to Industry

    Type
    Collection
    ...RESOLUTE collection AAV Packaging from Addgene Parkinson's Research from The Michael J Fox Foundation Search...Screening Messer and MJFF Created for research in Parkinson's Disease; For target validation and drug design...Recommended Applications PI Don't See What You're Looking For? Please let us know what you would like to ...

  7. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...Gene Vector Core (Link opens in a new window) have kindly deposited a series of lentiviral and adenoviral... College of Medicine Gene Vector Core have also kindly provided this protocol detailing the process for...gene of interest from the PGK (phosphoglycerate kinase I) promoter pCDH-CMV4 72284 Express gene of interest...

  8. Genetic Code Expansion

    Type
    Collection
    ...Genetic Code Expansion You may also like... GCE4All: Making Genetic Code Expansion Accessible Using Phosphoserine...with multiple copies of the tRNA. Applications By making small changes in selected amino acids within a ...there are several things to consider. If you are working off of a previously established protocol, make ...PylT_FLAG-AbKRS-chIPYE chimeric PylRS Chimeric photo-crosslinking-lysine Mammalian TAG Simon Elsaesser 140021 ...

  9. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...events and the systemic effects of Cre toxicity. Leakiness Leaky expression, where the gene of interest is...populations. Read our blog post about how to minimize leakiness in experiments using AAV vectors. Highlighted ...transfection efficiency, selecting desired clones, and making transgenic mice. ID Plasmid Description Gene/Insert...

  10. Luciferase Plasmid Collection

    Type
    Collection
    ...Renilla luciferase have short protein half-lives, making them useful as transcriptional reporters, but are...a secreted version of the protein is available, making it ideal for a variety of applications. To learn...expressed from a T7 promoter for normalization. John Atkins 174050 pGWB-nLUC Firefly Creation of N-terminal...

  11. Bacterial Expression Systems

    Type
    Collection
    ...NusA Cleavage sites: TEV protease, factor Xa, enterokinase, thrombin Signal peptides for periplasmic localization...Fluorescent Dyes and Ligands GFP Fusion Proteins — Making the Right Connection Photoactivatable Fluorescent... Engineering Modifying the bacterial genome by knocking out genes or introducing specific mutations can...

  12. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...dPspCas13b does not require a Protospacer Flanking Sequence (PFS), making it a very flexible editing system. ...

  13. Ginkgo Bioworks COVID-19 Collection

    Type
    Collection
    ...viral proteins as a first step for many different kinds of important experiments. In an effort to speed ...Codon-optimized expression (recode) Truncated for making pseudovirus (Ctrunc) ID Plasmid Description Mutations...

  14. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...plasmids and backbones with high cloning potential. By making these plasmids available as cgDNA, we hope to aid...assistance at [email protected] . Don’t See What You’re Looking For? At this time, Addgene has made a limited number...

  15. Plasmid Collections

    Type
    Collection
    ... page to help you find what you're looking for. If you are looking for a non-plasmid item, browse our ...

  16. Chemogenetics Plasmids

    Type
    Collection
    ...luciferase and a light-sensing optogenetic element, making a bimodal opto-chemogenetic actuator. When the ..."KORD AAV", "LMO", "Sternson", or other terms. Looking for viral preps? We also offer ready-to-use AAV...

  17. Optogenetics Plasmid Collection

    Type
    Collection
    ...engineered or identified to increase the spectral and kinetic properties as well as improve the expression and...Cre inhibition", "Boyden 2014", or other terms. Looking for viral preps? We also offer ready-to-use AAV...

  18. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...is a unique member of the CRISPR family. After nicking the target DNA, Cas3 initiates single-strand DNA...target DNA using a gRNA and recruits Cas3. After nicking the non-targeted strand, Cas3 starts unraveling...

  19. Resolute Plasmid Collection

    Type
    Collection
    ...Initiative 2 Joint Undertaking under grant agreement No 777372. This Joint Undertaking receives support ...
Showing: 21 - 40 of 88 results