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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 64 results for: NUC

Showing: 21 - 40 of 64 results

  1. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...associated reverse complement including additional nucleotides for cloning and expression purposes. NOTE: Here... BbsI restriction enzyme ( Table 3 ). Add a G nucleotide after the CACC sequence and before the 20-mer...pX330 vector is enhanced by the inclusion of a G nucleotide after the CACC sequence. Add a C at the 3’ end...Ligation Buffer, 6.5 μl ddH 2 O, and 0.5 μl T4 Polynucleotide Kinase (PNK) (10,000 U/ml). NOTE: Phosphorylated...

  2. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...EF1alpha addition of an mCherry reporter variant nuclear reporters via an H2B fusion shuttle plasmid for...design tools and ordered as standard desalted oligonucleotides with the appropriate overhangs: The plasmids...quick ligation of annealed and phosphorylated oligonucleotides containing the guide target sequence: Up to...

  3. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...recognizable sub-cellular localization (membrane, nucleus, etc.) are also available Non-toxic to living cells...pSNAPf-H2B Control Plasmid SNAP-tag Control for nuclear localization 101123 pSNAPf-ADRβ2 Control Plasmid...pCLIPf-H2B Control Plasmid CLIP-tag Control for nuclear localization 101130 pCLIPf-Cox8A Control Plasmid...

  4. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ..., additional nuclear localization signals, and two mutations in Cas9 to enhance nuclease activity; can...or Cas9 domains PE7 - addition of RNA-binding exonuclease protection factor La to PEmax to enhance pegRNA...

  5. Brain Armamentarium

    Type
    Collection
    ...10aa-H2B-WPRE3-BGHpA AAV vector for nuclear SYFP2 labeling in neurons. Useful for nuclear isolation and scRNA-seq ...SYFP2 (yellow fluorescent protein) plus separate nuclear FLAG epitope in striatal cholinergic neurons Jonathan...

  6. Antibody Guide

    Type
    Collection
    ...interactions between DNA and protein. Micrococcal nuclease digestion is used to shear the DNA in these assays...specific to your assay. Most IF techniques use a nuclear stain, such as DAPI, to identify individual cells...cells. Immunofluorescence experiments often use a nuclei marker to identify individual cells and additional... PE, and APC 4-color microscopy panel: DAPI for nuclei, AF488, AF555, and AF647 Antibody Selection Selecting...

  7. CRISPR Plasmids - Tagging

    Type
    Collection
    ... tested by fusing EGFP-2A-PuroR cassette to a nucleolar protein, fibrillarin (FBL). To repeat the knock-in...Cas to generate conditional alleles of essential nuclear and cytoplasmic proteins, which can then be depleted...articles: Kunzelmann, et al. G3 2016 Bottcher, et al. Nucleic Acids Res. 2014 The Förstemann lab has provided...

  8. CRISPR References and Information

    Type
    Collection
    ...outcomes from cleaving nucleases (e.g. Cas9, Cas12a) and noncleaving nucleases (e.g. base editing). Developed...an accession number, genomic location, pasted nucleotide sequence, or a sequence text file you upload....

  9. Plan Your Experiment

    Type
    Collection
    ...increase or decrease depending upon the specific nucleotides within the selected target sequence. Additionally...the Cas enzyme and gRNA, or as a functional ribonucleoprotein (RNP). Created with BioRender.com. When choosing...delivery Lastly, you can deliver Cas-gRNA ribonucleoprotein (RNP) complexes directly into your target ...

  10. Zinc Finger Consortium Reagents

    Type
    Collection
    ... method Nuclease Expression Vectors - Cloning vectors for expressing zinc finger FokI nuclease fusions...

  11. TALEN Expression Vectors

    Type
    Collection
    ...these vectors also have a FLAG epitope tag and nuclear localization signal at their amino-terminus. The...Assembly TALEN Kit . Vectors encoding wild-type FokI nuclease domains ( Sander et al., Nat Biotechnol. 2011 ...

  13. Retrovirus Plasmids

    Type
    Collection
    ...are only able to enter the nucleus during mitotic breakdown of the nuclear envelope). Additionally, γ-...

  14. Arf GTPase Family

    Type
    Collection
    ...centrosomes, the plasma membrane, lipid droplets, the nucleus, and mitochondria. The activity of GTPases is regulated... regulated both upstream (by GEFs, guanine nucleotide exchange factors), and downstream (by GAPs, GTPase...

  15. Zebrafish Plasmid Collection

    Type
    Collection
    ...site-specific recombination, morpholinos, zinc finger nucleases, TALENS, and CRISPR/Cas9-based gene editing make...Cas9 - Jeffrey Essner Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) expression plasmids - Keith Joung Lab CRISPR...

  16. TALEN Plasmids and Kits

    Type
    Collection
    ... plant pathogenic Xanthomonas spp. to the FokI nuclease, TALENs bind and cleave DNA in pairs. Binding ...DNA-binding domain of a TALE, along with strong nuclear localisation tags and repress transcription initiation...

  17. Neurodegeneration Research Collection

    Type
    Collection
    ...Find plasmids expressing HTT, C9ORF72, LRRK2, Synuclein, and more. New and Noteworthy: Use tau constructs...Noteworthy: Use a CRISPRi system to target alpha-synuclein. Sastre et al. Sci Rep. 2023 Oct 18. Target neural...

  18. Rett Syndrome

    Type
    Collection
    ...ranscriptional R epressor D omain (TRD) the TRD contains a Nuclear receptor Co-Repressor 1/Silencing Mediator of Retinoic...receptor (NCoR/SMRT) interacting domain (NID) a nuclear localization signal (not essential for localization...

  19. Deisseroth INTRSECT Collection

    Type
    Collection
    .... Dopamine-glutamate neuron projections to the nucleus accumbens medial shell and behavioral switching...Cortical-Thalamic-Striatal Circuits through the Parafascicular Nucleus. Neuron 102(3):636-652. PubMed (Link opens in a...

  20. Bacterial Expression Systems

    Type
    Collection
    ... screen the activity of arrays of zinc finger nucleases. Addgene Blog Introduction to FRET Bimolecular...activity Fluorescence (mCherry) Fusobacterium nucleatum Jörg Vogel 46002 pGR Terminator strength Fluorescence...
Showing: 21 - 40 of 64 results