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We narrowed to 66 results for: RON

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Showing: 21 - 40 of 66 results

  1. AAV Molecular Tools

    Type
    Collection
    ...used for neurophysiology experiments, including neuronal connectivity labeling. ID Name Expression System...TEVp EF1a-driven, Cre-dependent Cre-dependent, bicistronic expression of designer pro-taCasp3 and TEVp for..., Cre-dependent Inducing apoptotic ablation of neurons through a process reliant on Flp recombinase. 5...expression of Kir2.1 and mScarlet-KASH for silencing neurons. 2, 8 Ivo Spiegel 241241 GfaABC1D-Lck-mCherry-ibARK...direction across synapses. Also see Monosynaptic Neuronal Tracers available at Addgene. ID Name Expression...protein to express in presynaptic glutamatergic neurons, Cre payload. Must use with AAV8-DIO-Reporter viruses...protein to express in presynaptic glutamatergic neurons that express Cre, Flp payload. Must use with AAV8...

  2. Biosensor AAV Preps

    Type
    Collection
    ...Boyden Voltage Reporter: Voltron 119036 pAAV-hsyn-flex-Voltron-ST Syn Voltron-ST none Cre dependent 1 ...ST-WPRE Syn Voltron2-ST none Cre dependent 1 GENIE Project 217676 pAAV-EF1α-DIO-GtACR2-P2A-Voltron2ST-W3SL EF1a...Oxytocin (OT) Sensors GRAB_OT Voltage Sensors Archon Voltron JEDI-2P ASAP ATP Sensors iATPSnFR2 5-HT Sensors...pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3 Syn axon-GCaMP6s bicistronic mRuby3 Constitutive 5, 9 Lin Tian 112010 pAAV-hSynapsin1... EF1a Voltron2-ST none Cre dependent 1 Joseph Raimondo Voltage Reporter: JEDI-2P 179459 pAAV-EF1a-DIO-...

  3. Bacterial Expression Systems

    Type
    Collection
    ...activity Fluorescence (GFPmut3a) and luminescence (lux operon) Gram-negative bacteria Attila Karsi 14080 pAKlux2...pAKlux2 Promoter activity Luminescence (lux operon) Gram-negative bacteria Attila Karsi 14076 pAKgfp1 Promoter...pMV306hsp+LuxG13 Promoter activity Luminescence (lux operon) Mycobacterium sp. Brian Robertson , Siouxsie Wiles...pMV306hsp+Lux Promoter activity Luminescence (lux operon) Mycobacterium sp. Brian Robertson , Siouxsie Wiles...pMV306G13+Lux Promoter activity Luminescence (lux operon) Mycobacterium sp. Brian Robertson , Siouxsie Wiles...78565 pCM18 Promoter activity Luminescence (lux operon) Vibrio cholerae James Kaper 68374 PGR-Blue Terminator...

  4. Structural Genomics Consortium Plasmids

    Type
    Collection
    ...Resources SGC: www.thesgc.org SGC Toronto: www.thesgc.org/scientists/groups/toronto Protein Database (PDB): www.pdb.org...without restriction. Many SGC plasmids from the Toronto, Oxford and Stockholm sites have been deposited...the SGC Plasmid Collection By All Plasmids SGC Toronto SGC Oxford SGC Stockholm Bacterial Expression Plasmids...

  5. Optogenetics Plasmid Collection

    Type
    Collection
    ...specific neurons, often utilizing viral expression systems, to activate or deactivate these neurons in a .... When using these opsins as a tool to control neuronal activity, scientists generally attach a fluorescent... table below for the optogenetics plasmids for neuronal excitation and inhibition. For example, search...

  6. Luciferase Plasmid Collection

    Type
    Collection
    ...contain introns Anthony Leung Return to Top Expression Constructs Luciferase driven by a strong constitutive...reporter reporter vector allowing the study of biscistronic promoters. Daniel Christophe 119760 pSGDlucV3.0...expression of firefly luciferase and iRFP720 from a bicistronic promoter Antonius Plagge 72486 pCDH-EF1-Luc2-...for bioluminescent chemo-optogenetic control of neuronal signaling. TRUPATH Kit , ONE-GO Biosensors Kit...

  7. Adenovirus Plasmids

    Type
    Collection
    ... packaging capacity, high efficiency in driving strong transient gene expression in both dividing and ...production of viruses containing transgene under Neuron-specific enolase (NSE) promoter Bamburg 62621 pShuttle-CMV-F2A-T2A-Venus...pShuttle-CMV-F2A-T2A-Venus For production of viruses with multicistronic expression and a Venus tag Wang 50959 ShuttleMCP...-CMV-F2A-T2A For production of viruses with multicistronic expression Wang Return to top Adenoviral Plasmids...

  8. Antibody Guide

    Type
    Collection
    ... more expensive than polyclonal antibodies. Environmental factors, binding partners, location in the cell...antigen. Polyclonal antibodies are often more environmentally stable and more cost-effective. They are useful...are synthetic binding proteins which use the Fibronectin type III domain as their scaffolding instead ...does not use cross-linking and instead relies on strong interactions between DNA and protein. Micrococcal...for DNA:histone modifier interactions, which have strong binding between the protein and DNA. Some ChIP ...different antibodies and do batch validation up front rather than risk delaying a process through sequential...

  9. Viral Production

    Type
    Collection
    ...Edward Boyden (Addgene viral prep # 84445-AAVrg). Electron Microscopy The ratio of empty to full (i.e., genome...representative vector preparations was quantified with electron microscopy after negative staining. Empty vector...appear darker than full vector particles. Figure 3: Electron micrograph of AAV vector preparation shows that...psPAX2 and pMD2.G packaging system developed by the Trono lab . Cell culture medium (OptiPro SFM) containing...

  10. Rett Syndrome

    Type
    Collection
    ..., an immortalized pre-neuronal cell line that can be differentiated into neurons Coriell Institute: Patient-specific...the location and type of MECP2 mutation can be a strong predictor of symptom severity. Broadly, early gene...and cell-specific differences and correlate with neuronal maturation. Hum Mol Genet . 11, 115–124. PMID:...

  11. Caltech Systemic Capsids

    Type
    Collection
    ...tropism - AAV.MaCPNS1, AAV.MaCPNS2 Cell type-biased: Neurons - AAV.CAP-Mac, AAV9-X1.1 For marmosets: Broad tropism... tropism - AAV.CAP-B22 Cell type-biased: Neurons - AAV.CAP-B10 Brain vascular cells - AAV.CAP-Mac For ...: Broad tropism - AAV-PHP.eB Cell type-biased: Neurons - AAV.CAP-Mac Brain vascular cells - AAV9-X1.1,...animal Brain vascular cells - AAV9-X1.1, AAV1-X1 Neurons - AAV.CAP-B10, AAV-PHP.N Broad - AAV-PHP.AX with...

  12. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ... pseudotyped virus for SARS-CoV-2 and other coronaviruses using appropriate packaging plasmids. Use the...Nemazee lab - Pseudotyped virus protocol for coronaviruses (DOCX, 184 KB) Addgene Blog Posts Viral Vectors...COVID-19 Resources and Collections COVID-19 and Coronavirus Plasmids & Resources Ginkgo Bioworks COVID-19...

  13. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...filaments (neuronal) Nefm EGFP Anthony Brown 32909 pEGFP-mNFM Intermediate filaments (neuronal) Nefm EGFP...Kirk Mykytyn 104358 pEGFP-N-Drd1 Primary cilia (Neurons) Drd1 EGFP Kirk Mykytyn *Fusions to other FPs can...for delivering proteins to the extracellular environment. Proteins are first translated at the endoplasmic...

  14. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...Optogenetics Ed Boyden AV-1-PV3446 59170-AAV1 pAAV-Syn-Chronos-GFP Optogenetics Ed Boyden AV-1-PV3447 59171-AAV1...Optogenetics Ed Boyden AV-5-PV3446 59170-AAV5 pAAV-Syn-Chronos-GFP Optogenetics Ed Boyden AV-5-PV3447 59171-AAV5...-GFP Ed Boyden AV-9-PV3446 59170-AAV9 pAAV-Syn-Chronos-GFP Ed Boyden AV-1-PV3511 98926-AAV1 pAAV.CAG.GFPsm-myc.WPRE.SV40...Karl Deisseroth AV-8-PV3706 62726-AAV8 pAAV-Syn-Chronos-tdTomato Ed Boyden AV-1-35507 35507-AAV1 pAAV-Ef1a-DIO...

  15. Plant Plasmids and Resources

    Type
    Collection
    ...genetic modules for Golden-Gate cloning of synthetic operons into chloroplast transformation vectors. Golden... CRISPR/Cas9 constructs using a high efficiency intron-optimized SpCas9-coding gene in plants. CRISPR ...Kate Creasey Krainer , Vladimir Nekrasov , Nicola Patron Additional Genome Editing Resources Check our Plant...

  16. CRISPR Guide

    Type
    Collection
    ...general repair pathways: the efficient but error-prone non-homologous end joining (NHEJ) pathway and the...to the desired edit can increase efficiency. Synchronizing cells or modulating the cell cycle can help ...exhibit almost unidirectional insertions but are more prone to off-target insertions. And in the case of both...differentiation. The system is reversible, with a companion CRISPRon to undo repression using a TET1-dCas9 fusion. ... Gelsinger, D. R., Vo, P. L. H., Klompe, S. E., Ronda, C., Wang, H. H., & Sternberg, S. H. (2024). Bacterial.... 41 (7), 968–979. PMID: 36593413 Vo, P. L. H., Ronda, C., Klompe, S. E., Chen, E. E., Acree, C., Wang...

  17. Retrograde AAV viral preps

    Type
    Collection
    ...transgene delivery to projection neurons. Specific classes of projection neurons can be targeted by using this...Activator Optogenetics Edward Boyden 59170 pAAV-Syn-Chronos-GFP Syn Activator Optogenetics Edward Boyden 59171...

  18. Zhang Lab CRISPR Page

    Type
    Collection
    ...PX551; Truncated MeCP2 promoter-driven SpCas9; for neuronal expression 60958 : PX552; U6 promoter-driven; ...pAAV-pMecp2-SpCas9-spA) for expressing Cas9 in neurons. SpCas9 guide acceptor: The PX552 plasmid (#60958...-mediated expression of an sgRNA targeting the neuronal-specific gene NeuN. As a control they designed...

  19. Ginkgo Bioworks COVID-19 Collection

    Type
    Collection
    ...or sort buttons to find SARS-CoV-2 and common coronvirus plasmids based on: Gene insert (e.g. spike, envelope... Expression Systems Collection: COVID-19 and Coronavirus Plasmids & Resources , links to collections of...

  20. AAV Viral Preps

    Type
    Collection
    ...Purification, Cell Ablation, CRISPR Monosynaptic Neuronal Tracing Rabies Virus-Based Serotype Serotype Testing...retrograde Retrograde AAV To target projection neurons Caltech Systemic Capsids PHP.eB, PHP.S, PHP.V1,...
Showing: 21 - 40 of 66 results