We narrowed to 104 results for: SCR

...encoded on RNA. The genome includes a reverse transcriptase and an integrase to insert the viral genome ...plasmids that have inserts. ID Plasmid 5'LTR Description PI 1780 pBABE-neo largeTcDNA MoMLV Expression...cloning and gene expression. Select with puromycin or screen for GFP. David Bartel 32702 pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE...plasmid 12282 for the addition of WRE, a post-transcriptional regulatory element that increases export to...Gamma-Retroviral Packaging Plasmids ID Plasmid Description PI 8449 pUMVC Contains MoMLV gag and pol . Use...Gamma-Retroviral Envelope Plasmids ID Plasmid Description PI 1733 pCI-VSVG VSV-G recommended for the FELIX...

...expression plasmids. Large collections or kits are described below and not included in this table. For a more...search Addgene's entire collection. ID Plasmid Description Gene/Insert PI Plasmids Expressing Worm Genes...strains. CRISPR plasmids from John Calarco's lab as described in Heritable genome editing in C. elegans via ...genome-editing events. Developed in Craig Mello's lab and described in A Co-CRISPR Strategy for Efficient Genome Editing...also been developed by the Fire laboratory and described in Efficient Marker-Free Recovery of Custom Genetic...C. elegans and other nematodes. Wormbuilder - Describes methods to engineer the genome of the genetic ...

...allows you to fuse proteins of interest to transcriptional regulatory elements that will turn on gene ...and an engineered strain for a B2H system used to screen the activity of arrays of zinc finger nucleases...Propionate Escherichia coli Jay Keasling 113634 pSCrhaB2 PrhaB Rhamnose Burkholderia cenocepacia Miguel...nm) Escherichia coli Christopher Voigt 164226 pSCrhaB2plus PrhaBAD Rhamnose Burkholderia sp. Silvia Cardona...selection of plasmids using tetracycline-regulated transcriptional activation or repression. The BIOFAB plasmid...that element. When this activation requires a transcription factor to bind to a small molecule, the reporter...DNA-binding specificity of proteins such as transcription factors. Addgene Blog Luciferase Technologies...

...disease severity. The MECP2 protein is a global transcriptional regulator of thousands of genes and studies...studies have suggested roles in transcriptional repression, activity dependent de-repression, chromatin remodeling...DNA and recruit known co-repressors or other transcriptional modifiers suggests the major function of MECP2...NTD) the M ethyl B inding D omain (MBD) a T ranscriptional R epressor D omain (TRD) the TRD contains a...species (mouse or human) Mutation ID Plasmid Description Gene/Insert Mutations PI Return to top External...Developing Human Brain - includes developmental transcriptome information, prenatal LMD microarray data, ISH...

...Other Applications Screen CRISPR libraries are a powerful tool for genome-wide screening. Purify dCas9 fused...Prime Edit Cas nickase enzymes fused to reverse transcriptase install new genetic information into a specific...transposases insert large fragments of DNA. Transcriptional Regulation Activate dCas9 fused to an activator..., or fused to a repressor peptide, inhibits transcription. Epigenetics dCas9 fused to an epigenetic modifier...

...H134R)-EYFP Flp Yes 126080 pAAV-Ef1a-sCreDIO hChR2(H134R)-eYFP Scre No 126081 pAAV-Ef1a-vCreDIO hChR2(H134R...public folder (Link opens in a new window) of manuscripts that have used these constructs experimentally...Yes 55638 pAAV-EF1a-vCre None Yes 55635 pAAV-EF1a-sCre None No 55633 pAAV-EF1a-mCherry-IRES-Dre None No...in the Periaqueductal Gray Facilitate the Itch-Scratching Cycle via Article Tac1-Expressing Neurons in ...in the Periaqueductal Gray Facilitate the Itch-Scratching Cycle via Descending Regulation. Neuron 101(1...

...124606 SOX2-mEGFP AICSDP-60 mEGFP Transcription factor SOX-2 Transcription Factor 124607 ACTN2-mEGFP AICSDP...human cell lines of their choice. For a full description of how to use these plasmids, check out Plasmids...133963 UBTF-HaloTag AICSDP-80 HaloTag Nucleolar transcription factor UBF Nucleolus (fibrillar center) 133964...159745 TFAM-mEGFP AICSDP-72 mEGFP Mitochondrial transcription factor A (TFAM) Mitochondrial nucleoids 164499...

..., that will probably never happen. Yet with transcription activator-like (TAL) effectors, scientists in...that came out in the spring of 2011, the groups describe a set of customized plasmids that can be used ... a TALEN kit through Addgene. This kit, first described in Nature Biotechnology in August, uses a serial...in Nature Biotechnology early in 2011 and they described their kit for assembling TAL effector arrays in...sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Feb;29(2):149-53. PMID...

...Pooled CRISPR Libraries for Screening Use pooled CRISPR libraries to screen for genes involved in specific... Guide to Plasmid Pooled Libraries . ID Name Description Genes/Insert Selection PI Human knockout pooled...be fused to a transactivator and used as a transcriptional activator . ID Name Insert Antibiotic Resistance...2A-Blast-WPRE) Zhang Pooled Barcoding Libraries ID Name Description PI 115643 Morris CellTag Barcode Library Version...

...Salvador family WW domain containing protein 1 SCRIB Scribbled planar cell polarity protein SHC SHC1 SHC2 ...specificity phosphatase E2F E2F1 E2F2 E2F3 E2F transcription factor ECT2 Epithelial cell transforming 2 EGFR...protein kinase ETS ETS1 ETS2 ETS proto-oncogene, transcription factor EXOC EXOC1 EXOC2 EXOC3 EXOC4 EXOC5 EXOC6...TBK1 TANK binding kinase 1 TFDP TFDP1 TFDP2 Transcription factor Dp TIAM TIAM1 TIAM2 T-cell lymphoma invasion...

...TFORF3549 3rd For barcoding transcription factors for identification in pooled screens. Part of the MORF Collection... for gRNA expression . ID Plasmid Generation Description PI 8453 pLKO.1 puro 3rd U6-driven shRNA empty...Lentiviral Packaging Plasmids ID Plasmid Generation Description PI 1732 pCPRDEnv 2nd Part of the FELIX vector...Popular Lentiviral Envelope Plasmids ID Plasmid Description PI 1733 pCI-VSVG VSV-G recommended for the FELIX...

...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors By Species...plasmids and protocols needed for using them, are described below. Read our CRISPR 101 post for more information...CRISPR-Cas tagging system were tested by tagging transcription factors with FLAG in human cell lines. To repeat...targeting vector that encodes both a guide RNA transcript and a repair template for an individual tagging...

...nuclear transcription factor Y, alpha CBF-A, CBF-B, FLJ11236, HAP2, NF-YA NFYB nuclear transcription factor...RAET1G retinoic acid early transcript 1G ULBP5 RAET1L retinoic acid early transcript 1L - RELB v-rel reticuloendotheliosis...endothelin receptor type B ABCDS, ETB, ETBR, ETRB, HSCR, HSCR2 FGF10 fibroblast growth factor 10 - FGF2 fibroblast...endothelin receptor type B ABCDS, ETB, ETBR, ETRB, HSCR, HSCR2 EGF epidermal growth factor (beta-urogastrone...immune response. Over 60 interleukins have been described. NK Cell Signaling Natural killer (NK) cells can...beta CBF-A, CBF-B, HAP3, NF-YB NFYC nuclear transcription factor Y, gamma CBF-C, CBFC, DKFZp667G242, FLJ45775..., PA28G, REG-GAMMA RAET1E retinoic acid early transcript 1E LETAL, MGC125308, MGC125309, RAET1E2, ULBP4...

...zebrafish (can be used as a substitute for pMLM335 described in Foley et al., PLoS One 2009) pMLM292 (for expression...zebrafish (can be used as a substitute for pMLM336 described in Foley et al., PLoS One 2009) pMLM800 (for expression...Methods 2011 ) in mammalian cells or zebrafish as described by Moore et al. PLoS One 2012 and Cade et al.,...

...example, search "Cdc42", "transcription", or other terms. ID Plasmid Description Gene/Insert Vector Type...many different types of proteins. For a full description (including peak response spectra) of different...preparations of many optogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter Eligible for...

... is described in more detail in the Multiplex Assays section below. The following table describes the ...of B cells isolated from animals immunized as described above are used to create hybridomas, which produce...each antibody has two binding sites. Isotype Description Configuration and Valency Subclasses IgA Prevalent...primary antibody for your protein of interest, as described in the IP section above. Reverse the crosslink...validation can be done using methods such as transcriptomics or proteomics. This approach is often appropriate...

...replace” gene editing method in which a reverse transcriptase (RT) is fused to the C terminus of Cas9 H840A...edit is incorporated into the DNA by reverse transcription. The edited DNA strand displaces the unedited...Moloney murine leukemia virus (M-MLV) reverse transcriptase. Engineering of the PE enzyme and related components...including exploring new sources for the reverse transcriptase. Many PE enzymes and related tools are available...

... Large collections or kits are described below . ID Plasmid Description Gene/Insert PI Return to top Highlights...that are amenable to genetics, high-throughput screening and non-invasive live imaging. A standard toolbox... Amro Hamdoun Lab. A useful “starter kit” for screening fluorescent protein fusion expression in organisms...

...blood brain barrier integrity, and using CRISPR screens to understand more globally how neurons function... et al. Cell. 2021) Conduct multimodal genetic screens in human iPSC-derived neurons with a CRISPRi-based...plasmids expressing SOX10, OLIG2, and NKX6.2 transcription factors for rapid and efficient generation of...astrocytes to dopaminergic neurons using three transcription factors in a single polycistronic lentiviral...

... on Request for many more. Metal Ions Target Description Article PI Calcium jGCaMP8 Fast Genetically Encoded...Yan Qin Return to top Small Molecules Target Description Article PI Acetyl-CoA PancACe acetyl-CoA sensor...Frommer Return to top Neurotransmitters Target Description Article PI Acetylcholine Family of ACh sensors...Clapham Return to top Cell Physiology Target Description Article PI Autophagy Measure autophagic flux ...Near-infrared fluorescent voltage indicator nirButterfly Screening and Cellular Characterization of Genetically Encoded...caspase", "mTORC1", "mitochondria"). ID Plasmid Description Gene/Insert Vector Type Eligible for Viral Packaging...