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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 93 results for: Tes

Showing: 21 - 40 of 93 results

  1. Plan Your Experiment

    Type
    Collection
    ...partial homology to additional sites throughout the genome. These sites are called off-targets and should...efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing. Scientific...Once possible PAM sequences and putative target sites have been identified, it is time to choose which... gRNA target sequence does NOT match additional sites within the genome. In a perfect world, your gRNA...examined during gRNA design. In general, off-target sites are not cleaved as efficiently when mismatches occur...

  2. Zebrafish Plasmid Collection

    Type
    Collection
    ...function of zebrafish astrocytes. Knock-in tagging - Michel Bagnat lab. Targeting cassettes encoding fluorescent...Zebrafish Society (IZFS) - An organization that promotes and advocates for zebrafish research in the international...Morsch Lab. A vertebrate model to study ALS-linked aggregates in real time. Live imaging of astroctyes - Kelly...Resources Zebrafish Genome Project - Access the latest reference sequence assembly for the zebrafish genome...

  3. CRISPR References and Information

    Type
    Collection
    ...access tool from Synthego allows you to determine rates of CRISPR editing at a given locus. To use the tool... window) A tool that identifies putative target sites for S. pyogenes Cas9, S. aureus Cas9, S. thermophilus...probability of off-target effects. Cas-Designer integrates information from Cas-OFFinder and Microhomology...a new window) Identifies candidate sgRNA target sites by off-target quality. Validated for gene inactivation...in a new window) Identification of sgRNA target sites and off-target evaluation for base edits using adenine...window) Can be used to identify novel gRNA target sites in a single gene, as well as a target site common...

  4. NETRF

    Type
    Collection
    ...Institute Identifying Altered Epigenetic States and Drivers in Intestinal Carcinoid and Pancreatic Neuroendocrine... Cancer Institute Finding the Causes of Small Intestinal Neuroendocrine Tumors Ramesh Shivdasani Dana-...Dana-Farber Cancer Institute Epigenetic Regulators of Intestinal Endocrine Cells and Carcinoid Tumors Qiao Zhou...Zhou Harvard University Epigenetic Regulators of Intestinal Endocrine Cells and Carcinoid Tumors Bradley ...

  5. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...simultaneously with dual labeling when used with two substrates Can be used in live or fixed cells, and for internal...protein tags based on the acyl carrier protein. Substrates are derivatives of Coenzyme A (CoA). These tags... Please see the note below for information on substrates and synthases needed for use of these tags. Plasmids...product portfolio, including the CoA-derivative substrates and the ACP and SFP Synthases. Users may still... their own fluorescent or non-fluorescent CoA substrates by purchasing coenzyme A disodium salt from a...

  6. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...pyogenes GFP Vakoc U6>sgRNA(F+E) 59986 Other/Ciona intestinalis BsaI none S. pyogenes Christiaen p(U6a-BsaI-...vectors containing multiple guide RNA expression cassettes and a Cas9 nuclease/nickase expression cassette...the expression of multipe gRNAs. Csy4 cleavable cassettes; requires expression of Csy4 (eg Plasmid 53369...gRNA module vectors, multiple gRNA expression cassettes can be assembled using Golden Gate cloning or ...polycistronic glycine tRNA-gRNA genes (PTGs) and then PTG cassettes are assembled into a Cas9 expressing vector. Yang...to rapidly generate multiple sgRNA expression cassettes, which can be assembled into the binary CRISPR...sgRNAs Zebrafish Plasmids for assembly of gRNA cassettes using Golden Gate ligation into Tol2-based pGGDestTol2LC...

  7. TALEN Guide

    Type
    Collection
    ...nuclease operates as a dimer, cutting in the spacer region between two distinct ZF target sites. (B) A ...species almost as easily as correcting a typo in the latest draft of your dissertation. Well, that will probably...corresponding zinc fingers, potential targetable sites in your average genome are every 500 bp. TAL effectors... a T), but they still have potential targetable sites approximately every 35bp. Researchers are still ...

  8. CRISPR Plasmids - Tagging

    Type
    Collection
    ...sequences and gRNA target sites. The first deposited PITCh plasmids were tested by fusing EGFP-2A-PuroR ...plasmids in this CRISPR-Cas tagging system were tested by tagging transcription factors with FLAG in human...plasmids in the following articles provide PCR templates for amplification of the tag (eg GFP, Flag, YFP...elegans . This system uses a 10-day protocol, generates “clean” homozygous mutants with no co-integrated...

  9. Caltech Systemic Capsids

    Type
    Collection
    ...Cell type-biased: Astrocytes - AAV-PHP.AX with GRE or transgenic animal Oligodendrocytes - AAV-PHP.eB with...digital PCR, in vitro and (when possible) in vivo testing, and full sequencing of the final viral vector ...LY6A-nonpermissive mouse strains. **AAV1-X1 has not been tested in rats. Abbreviations: AAV, adeno-associated virus...regulatory element; NHP, non-human primate. Image courtesy of the Gradinaru Lab and the Caltech CLOVER Center...

  10. Immunology Research Plasmids and Resources

    Type
    Collection
    ...inducible - VIP vasoactive intestinal peptide MGC13587, PHM27 VIPR1 vasoactive intestinal peptide receptor 1 ...immune response is triggered within the first few minutes of exposure to a pathogen and is the dominant defense...Chemokines recruit innate and adaptive immune cells to sites of inflammation throughout the body. Cytokines Cytokines...its cognate antigen along with co-stimulation activates the T cell, whereas T cell tolerance or anergy... NK cells, macrophages, dendritic cells, and granulocytes. TLR The toll like receptors (TLR) play a key...Chemokines recruit innate and adaptive immune cells to sites of inflammation throughout the body. Symbol Name...chemokine (C-C motif) ligand 5 D17S136E, MGC17164, RANTES, SCYA5, SISd, TCP228 CCL7 chemokine (C-C motif)...

  11. TALEN Engineering

    Type
    Collection
    ... TALE transcriptional activator target sites and then generates individual user-friendly graphical roadmaps... ( Sander et al., Nat Biotechnol. 2011 ) Rats ( Tesson et al., Nat Biotechnol. 2011 ) Human somatic cells...monomeric TALE transcriptional activator target sites and to simplify the construction of TALEN expression...

  12. Antibody Production

    Type
    Collection
    ...Quality Control Each lot of antibody undergoes testing, either in-house or through outside labs, to confirm...storing at -20 °C. Each antibody catalog item is tested for shipping stability by incubating at 37 °C for..., freezing at -80 °C, thawing on ice, and then testing for loss of potency against a non-treated control...recommended assays. If an antibody has passed stability testing, it may ship at room temperature. However, Addgene...

  13. Plant Plasmids and Resources

    Type
    Collection
    ... easy assembly of one or more gRNA expression cassettes - Qi-Jun Chen. Cas9 and gRNA backbone for use...create plant expression vectors containing several cassettes and generate multi-construct transgenic plants...The ABRC collects, preserves, reproduces, and distributes diverse seed and other stocks of Arabidopsis ... (Link opens in a new window) - The initiative promotes interdisciplinary exchange, open technologies,...

  14. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and ...hypermutation in a 100 bp window. This technique creates diverse populations of mutants for directed evolution...adenosine deaminase to catalytically dead Cas13b creates a programmable RNA editor that converts adenosine... transcription factor and other protein binding sites. ID Plasmid Gene/Insert Promoter Selectable Marker...

  15. Viral Production

    Type
    Collection
    ...The data is also used to determine recombination rates in FLEX/DIO/fDIO constructs. Endotoxin Endotoxin...sterility. Transducibility Some viral vectors are tested in vitro and in vivo for gene expression and/or...line was obtained from Takara, and is routinely tested for mycoplasma contamination using mycoplasma detection... 2 weeks post-thaw, cell culture supernatant is tested for mycoplasma contamination. To date, Addgene ...

  16. Brain Initiative Collection

    Type
    Collection
    ...ID Plasmid Description PI Viral Preps Addgene distributes ready-to-use viral preps made from plasmids in...and targeted to the neuronal soma and proximal dendrites in a viral vector Hillel Adesnik 107708-AAV9 pAAV-hSynapsin...ChroME targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a ...and targeted to the neuronal soma and proximal dendrites under the control of internal ribosome entry sequence...recombination Ofer Yizhar Antibodies Addgene distributes ready-to-use recombinant antibodies made from...

  17. AAV Viral Preps

    Type
    Collection
    ...includes titering, purity assessment, sterility testing, and viral genome sequencing (VGS). In-stock AAV...Neuronal Tracing Rabies Virus-Based Serotype Serotype Testing Samples Small samples of control AAV vectors for...CAP-B10, CAP-B22 University of Florida Serotype Testing Panel for the Eye and Brain For enhanced transduction...

  18. All Antibodies

    Type
    Collection
    ... All Antibodies All Antibodies Addgene distributes ready-to-use recombinant antibodies. These monoclonal...recommended applications based on our in-house testing and data provided by trusted outside labs – with... represent the official views of the National Institutes of Health. Need Some Help? Browse some of our...

  19. Adenovirus Plasmids

    Type
    Collection
    ...plasmid, are recombined into a DNA molecule that incorporates sequences from both plasmids. This DNA molecule...for the AdenoBuilder genome assembly system; consolidates the regions present in pAd5-B6 ∆E3-GFP and pAd5... Vogelstein 16407 pAdEasy 2-GFP beta-gal Shuttle Test plasmid that contains β-gal and GFP; contains ∼10Kb...

  20. AAV Packaged on Request

    Type
    Collection
    ...such as titering, purity assessment, sterility testing, and viral genome sequencing.... process: Titering Purity assessment Sterility testing Viral genome sequencing (VGS) Find detailed pricing...Addgene does not perform functional or in vivo testing. We recommend that you consult the original paper...
Showing: 21 - 40 of 93 results