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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 89 results for: clu

Showing: 21 - 40 of 89 results

  1. Plan Your Experiment

    Type
    Collection
    ...expanded the CRISPR toolbox to include many different types of edits, including point mutations, base changes...Learn how to plan a basic CRISPR experiment, including selecting the edit type, designing and cloning...VP64) Many different activator systems exist, including the multi-plasmid SAM system Activate Design and...

  2. Serotype Testing AAV

    Type
    Collection
    ...promoter. For information about each catalog item, including the titer and quality control, visit the material... to Cart *Each viral service request will also include virus associated DNA (vdna), which is a sample ...

  3. Synthetic Biology - Overview

    Type
    Collection
    ... related to cloning and genomic modification, including shuttle, integration, reporter, and tagging vectors... occurring and synthetic regulatory elements, including promoters, terminators, repressors, activators...Addgene's collection of engineered bacterial strains, including plasmid-strain functional pairs. Browse Plasmids...

  4. Adenovirus Plasmids

    Type
    Collection
    ...humans. Advantages of adenoviruses in research include their large packaging capacity (>8 kb), high titers...the host genome. Disadvantages of adenoviruses include transient transgene expression, as well as the ... in either mammalian cells or microorganisms, including bacteria and yeast. Two plasmids, termed a shuttle...

  5. Retrovirus Plasmids

    Type
    Collection
    ...Learn about retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids...and several structural and enzymatic proteins, including reverse transcriptase and integrase. Once in a...creation of immortalized cells; additional plasmids include Puro resistance and Neo resistance Weinberg 10676...

  6. Antibody Plasmid Collection

    Type
    Collection
    ...Addgene's collection of plasmid-based antibodies, including monoclonal antibodies, scFvs, and nanobodies. ...Traditional sources of antibodies for research purposes include animals or hybridoma cells, but are difficult to...cells, yeast, plants, or insect cells. Benefits include consistency between lots and the ability to optimize...

  7. Promega Plasmid Collection

    Type
    Collection
    ...Industry Plasmid Technology Overview This collection includes specialized Promega technologies that support ...support a range of applications. The technology includes NanoLuc, HaloTag, SmBiT, LgBiT, HiBiT, and more. Luciferase...optimized for various protein expression systems including E. coli , mammalian cells, and cell-free systems...

  8. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...compartments or organelles. Addgene’s database includes many fluorescent biosensors that signal the presence...processes. Note that many of the articles linked below include variants with different binding affinities or negative-control...calcium indicators for optical imaging (GECOs), including blue, green, red, and ratiometric indicators An...biosensors. The ScEnSor Kit from the Olsson laboratory includes eight fluorescent reporters to characterize the...cerevisiae . The MOSAIC Kit from the Cohen laboratory includes 35 plasmids expressing a variety of fluorescent...

  9. Zebrafish Plasmid Collection

    Type
    Collection
    ...for human disease and translational research, including the study of cancer, inflammation and infection...contain tools for use in zebrafish research. This includes plasmids for genome editing, empty backbones, ...bearing either amino- or carboxyl-terminal tags, including Myc, HA, Flag, GST and eGFP epitopes. Zebrafish...

  10. Lentivirus Plasmids

    Type
    Collection
    ...cloning plasmid 3rd U6-driven shRNA empty plasmid; includes a stuffer for easy cloning Root 14748 pLKO.3G ...mouse U6 promoter; CMV-EGFP reporter cassette is included to monitor expression Parijs 11619 pLB 3rd Modification...mouse U6 promoter; CMV-EGFP reporter cassette is included to monitor expression Kissler 1730 pLionII 3rd...

  11. Immunology Research Plasmids and Resources

    Type
    Collection
    ...is not specific to any particular pathogen. It includes physical barriers such as the skin and mucosa,...adaptive immune response is antigen-specific and includes cellular and humoral immunity. Once an adaptive...the surface of antigen presenting cells (APC) including dendritic cells and macrophages. Antigen presentation...through effects on numerous immune cell types including T cells and B cells. It also impacts the development...development and function of innate immune cells including NK cells, macrophages, dendritic cells, and granulocytes...

  12. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...TALENs. CRISPR: An Adaptive Immune System CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat...allowing for precise gene editing. These edits can include mutations seen in human patients, protein tags,...off-target editing rates. Advances in this area include: Nickase mutant: cuts only one strand of DNA and...

  13. Bacterial Expression Systems

    Type
    Collection
    ...used tags, cleavage sites, and signal peptides include: Epitope tags: 6xHis, Flag, Strep II, c-Myc, HA...with different purification tag combinations, including PelB tags for localization to the periplasm. The...Guide to find an extensive collection of FPs, including photoactivatable, photoconvertible, and photoswitchable...

  14. Tetracycline Inducible Expression

    Type
    Collection
    ... sensitivity, and control of expression. These include: Tet-On Advanced : improved transactivator (rtTA-Advanced...occasionally, use Tet-On: express an rtTA or TetR and include a tet-responsive promoter upstream of your gene...off occasionally, use Tet-Off: express a tTA and include a tet-responsive promoter upstream of your gene...

  15. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...which includes all viral vectors transferred from the Penn Vector Core. Chemogenetics (including DREADDs...Addgene partnership to provide AAV viral preps, includes listing of transfer status for viral preps. Viral...

  16. Synthetic Biology - Networks and Gene Regulation

    Type
    Collection
    ...related to Networks and Regulation. The collection includes both naturally ocurring and synthetic regulatory...circuits, and higher level gene networks. Examples Include: promoters and terminators repressors and activators...

  18. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...plasmids for targeted epigenetic modification, including histone acetylation/demethylation, and cytosine...your gene of interest. Available modifications include: histone acetylation by p300 histone demethylation...

  19. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...CRISPR plasmids for targeting RNA sequences, which include the enzymes C2c2/Cas13a and Cas13b....Tools CRISPR Blog Posts Type VI CRISPR systems, including the enzymes Cas13a/C2c2 and Cas13b, target RNA...

  20. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...collection. The plasmids available in this format include a variety of popular plasmids and backbones with...order will need to be completed independently including the check out and will require separate shipping...
Showing: 21 - 40 of 89 results