...Gene/Insert Selectable Marker PI Publication Base Edit Catalytically dead dCas9 fused to a cytidine deaminase...Insert Promoter Selectable Marker PI Publication Activate Catalytically dead dCas9 fused to a transcriptional...Insert Promoter Selectable Marker PI Publication Interfere Catalytically dead dCas9, or dCas9 fused to a transcriptional...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Prime Edit Cas9 H840A nickase fused to a reverse...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Empty gRNA Expression Vectors Select a gRNA expression...

... any application Balanced Salt Solution (BSS) An ophthalmic solution suitable for any application TMN200...When using the AAV2(Y444F) serotype in future publications, please acknowledge Arun Srivastava and cite...When using the AAV2(trpYF) serotype in future publications, please acknowledge Arun Srivastava and cite... using the AAV2(4pMut)dHS serotype in future publications, please acknowledge Shannon Boye and cite Boye...using the AAV6(dbY-F+T-V) serotype in future publications, please acknowledge Arun Srivastava and cite...Strategies for Overcoming Donor-Variation and Implications in Genome Editing. Sci Rep . Oct 19;6:35495....

...it's usually more efficient than plasmid donor. Typical design is to have 50-80bp of homology arms on each... changes (>100bp insertions or deletions), we typically use plasmid donor, with two homology arms on each...transfection in cells like HEK, we use around 400ng of a typical size (~ 5kb) donor plasmid with Cas9/sgRNAs vector...plasmids. For ssDNA oligo design, the Zhang lab typically uses around 100-150bp total homology. The mutation..., giving 50-75bp homology arms. The Zhang lab typically use PAGE purified long oligos. For large changes...100bp insertions or deletions), the Zhang lab typically uses a plasmid donor, with two homology arms on...like HEK, the Zhang lab uses around 400ng of a typical size (~5kb) donor plasmid with Cas9/sgRNAs vector...

...the most abundant forms of posttranslational modifications in cells and research into its many roles in...Yale have made improvements to this system by genomically recoding E.coli (Lajoie et al., Science 2013)...of the popular SepOTSλ ( 68292 ). An important technical note: pSerOTS-C1* ( 188537 ) is a ROP minus plasmid...Barber et al, Biochemistry 2018). Iterative Synthetically Phosphorylated Isomers (iSPI). This new resource...WW2 domain) References & Protocols Iterative Synthetically Phosphorylated Isomers (iSPI) described in: ...an orthogonal translation system with enhanced biological tolerance. Mohler K, Moen JM, Rogulina S, Rinehart...phosphopeptides in bacteria for proteome-wide identification of phosphorylation-dependent interactions. ...

...cell, and specifically targets the starter cell via the EnvA receptor. RABV is able to replicate in the ...which is not required for the transcription or replication of the genome within infected cells, but is required...present, the virus can spread from this cell to synaptically connected cells (meaning, it can spread to cells... cells that lack the G protein, the virus can replicate but the newly formed viruses are unable to infect...complementation, G-deleted rabies virus can spread to monosynaptically connected cells. To further refine the targeting... was modified so that it could only infect a genetically specified neuronal population. This is done using...leukosis virus. EnvA can direct virus infection specifically into cells that express the TVA viral receptor...

...loci seem to be associated with the disease and implicate a wide range of cellular processes. The vast majority...’s disease (PD) is a chronic and progressive neurological disorder that primarily affects movement. More... consists mainly of managing symptoms through medication and surgery. PD primarily involves the malfunction...available from Addgene, sortable by type of genetic modification and target species. You can also find our guide...Sun et al. Nat Methods. 2020 Oct 21. Glutamate indicators with improved activation kinetics and localization... transcription factors for sensory, motor, and cortical neuron differentiation. Held et al. Cell Rep. ...A multicolor atlas for whole-brain neuronal identification in C. elegans. Yemini et al. Cell. 2021 Jan...

...implementation letter DNA amplification Viral vector production Density centrifugation purification Addgene’s comprehensive...preps, which includes MTA facilitation, DNA amplification, and high-quality quality control measures such...preparations, we set both minimum standards and typical titers, though actual yields may vary by construct...construct. Size Quantity Total Volume Minimum Titer Typical Titer Small 10 × 100 µL aliquots 1 mL 4 × 10 12... with a minimum titer of 2 × 10 12 GC/mL and a typical titer of 7 × 10 12 GC/mL. For more details, see...remain subject to the terms and conditions of the applicable transfer plasmid. We will help you gain legal...providing scientist through an MTA. This process typically takes 1–4 days, depending on your institution....

...vector preparations were produced with the pUCmini-iCAP-PHP.eB plasmid (Addgene #103005) . Note on PHP.eB...vector preparations were produced with the pUCmini-iCAP-PHP.S plasmid (Addgene #103006) . Browse Available...vector preparations were produced with the pUCmini-iCAP-PHP.V1 plasmid (Addgene #127847) . Browse Available...vector preparations were produced with the pUCmini-iCAP-AAV.MaCPNS1 plasmid (Addgene #185136) . Browse Available...vector preparations were produced with the pUCmini-iCAP-AAV.MaCPNS2 plasmid (Addgene #185137) . Browse Available...vector preparations were produced with the pUCmini-iCAP-AAV.CAP-B10 plasmid (Addgene #175004) . Browse Available...vector preparations were produced with the pUCmini-iCAP-AAV.CAP-B22 plasmid (Addgene #175005) . Browse Available...

...Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter PI Publication Drosophila ID Plasmid...Promoter PI Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication C. elegans...Promoter PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...Protocols gRNA Design Tools CRISPR Blog Posts Catalytically dead dCas9 fused to a transcriptional activator...

...small biomolecules or other physiological intracellular processes with genetically encoded fluorescent biosensors...mammals. Regulate Biological Activity Use fluorescent proteins to modulate biological activity, like transcription...Use this guide to learn about the applications of fluorescent proteins and to find the plasmids that ... Use this guide to learn more about the many applications of fluorescent proteins (FPs) and to find the...Plasmids | Guide Use small molecules to activate genetically engineered cellular receptors that affect signalling...Resources FPbase from Talley Lambert at Harvard Medical School Fluorescent Biosensor Database designed ...

...Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Plant ID...Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Zebrafish...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...editors are created by fusing Cas9 nickase or catalytically inactive “dead” Cas9 (dCas9) to a cytidine deaminase...and are thus well suited to directed evolution applications. Examples of these base editing systems include...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have suggestions for other plasmids that...

...antibodies are produced in-house and undergo application-specific validation and quality control by Addgene... Recombinant Antibody Reactivity Recommended Applications PI Don’t See What You’re Looking For? Please...within 1 business day regarding your account verification. Once your account has been verified, you will.... Pricing Addgene is a nonprofit organization dedicated to providing the scientific community with open...access to plasmid research tools. Pricing will be indicated on the product pages after you login to your account...depositing institution(s). It governs the transfer of biological materials and intellectual property rights, and... the depositing institutions. These agreements indicate the language which we are allowed to use for distribution...

...MoClo : Modular cloning for applications like recombinant protein purification and cell-free circuit design...distributes cannot be used to reconstitute self-replicating microbes or recreate the diseases they cause....our Search page to look for specific genes or applications that are not listed here. Addgene Microbiology...Prions Plasmids for Protozoa Species Entamoeba histolytica Leishmania sp. Plasmodium sp. Toxoplasma gondii...vaccinia virus - Bennink and Yewdell Labs Yarrowia lipolytica Golden Gate tool kit : Integrative vector assembly...transcription units in the oleaginous yeast Y. lipolytica - Rossignol Lab Yeast Gateway kit : 285 vectors...fluorescent protein plasmid collection is organized by application and by color. External Resources European Saccharomyces...

... by Talley Lambert at Harvard Medical School Zeiss Microscopy : Optical Highlighters NIC Wiki PSFP : Nikon...545-51 Shcherbakova et al. : Journal of the American Chemical Society, May 2012, Vol. 134 No.18, pp. 7913...Nature Communications, November 2012, Vol. 3 No. 1204 Wiedenmann et al. : Journal of Biomedical Optics...Monomer? Background Fluorescent proteins are genetically encoded tools that are used extensively by life... proteins created by Talley Lambert at Harvard Medical School More References below Jump to: Blue/UV Cyan... tagging with a wide variety of colors, some specifically optimized for yeast Lindquist Lab Vectors - ... Vol. 11 No. 5, pp.572-8 Yu et al. : Nature Communications, May 2014 Vol. 15 No. 5, p. 3626 Do you have...

...Gene/Insert Vector Type Promoter PI Publication Return to top Optical Switch Plasmids Browse, sort, or search...optogenetics plasmids such as microbial opsin and optical switch tools to measure and manipulate cells and...Fluorescent Protein Guide Microbial Opsin Plasmids Optical Switch Plasmids Background Optogenetics plasmids...proteins. These fusions can then be used as an optical switch to control protein interactions, dimerization...response spectra) of different types of opsins and optical switches available in these tables, refer to Addgene's...Description Gene/Insert Vector Type Promoter PI Publication Return to top Do you have suggestions for other...

...antibodies are produced in-house and undergo application-specific validation and quality control by Addgene...and time. An alternative approach is to use synthetically produced recombinant antibodies, created by ...extensively validated for neuroscience research applications from the NeuroMab/Trimmer Lab Recombinant mAb...imaging. Learn more about antibodies and their applications in our Antibody Guide ! Cloning and Expressing...antibodies, and Fc-silent mutants. Functional diversification of hybridoma-produced antibodies by CRISPR/...456(1):527-33. Gavin Wright pET-30-based vector dedicated to efficient scFv expression, which circumvents...and Generation of synthetic nanobodies against delicate proteins. Nat Protoc. 2020 Apr 8. Markus Seeger...

...55632 pAAV-Ef1a-mCherry-IRES-Cre EF1a Cre and physically-separate mCherry expression Recombinases Deisseroth...55634 pAAV-EF1a-mCherry-IRES-Flpo EF1a Flpo and physically-separate mCherry expression Recombinases Deisseroth...AAV-hSyn1-GCaMP6f-P2A-nls-dTomato Syn GCaMP6f and physically separate nuclear dTomato Calcium sensor Ting ...-DIO-GCaMP6f-P2A-nls-dTomato EF1a GCaMP6f and physically separate nuclear dTomato, Cre-dependent Calcium...AAV-hSyn1-GCaMP6s-P2A-nls-dTomato Syn GCaMP6s and physically separate nuclear dTomato Calcium sensor Ting ...sensor Looger 113050 pAAV-hSyn-GRAB_DA1h Syn genetically-encoded fluorescent dopamine(DA) sensor GRAB_...Dopamine sensor Li 123308 pAAV-hSyn-GRAB_NE1m Syn genetically-encoded fluorescent norepinephrine(NE) sensor...

...required for viral replication. Thus, as a safety feature, viruses used in research typically do not have the...are the most popular virus for gene transfer in clinical trials. These viruses have a relatively high transduction...Overview of Viral Plasmids While plasmids are typically used to encode, propagate, and manipulate genetic...Instead, the genes that are required for viral replication are usually divided among many plasmids, such...plasmids, however, retains the properties of any typical plasmid and thus can be easily modified and amplified... For example, adeno-associated virus (AAV) is typically preferred for in-vivo studies because of its low...

... nuclease along with the guide RNA. For most applications, we recommend using Cas9 with the single guide...BbsI, and a pair of annealed oligos (design is indicated below) can be cloned scarlessly into the vector...BbsI, and a pair of annealed oligos (design is indicated below) can be cloned into the CRISPR array. The...- sgRNA and SpCas9 together 2 vector system - lentiCas9-Blast and lentiGuide-Puro (sgRNA) Browse the Addgene... genes. It consists of three components: A nucleolytically inactive Cas9-VP64 fusion A sgRNA incorporating...function of genetic elements. To broadly enable the application of Cas9 in vivo and ex vivo , the Zhang Lab established...can be combined with Cas9 in a wide range of applications. Full references are below. The Cas9 knockin...

...Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter PI Publication Plant ID Plasmid ...Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...Protocols gRNA Design Tools CRISPR Blog Posts Catalytically dead dCas9, or dCas9 fused to a transcriptional...