... display less off-target activity. High fidelity Cas9s: Three high fidelity Cas9 variants , SpCas9-HF,...95% of archaeal and 48% of bacterial genomes, are highly diverse, with varying PAM sequences and number/...) Type V (Cpf1) Type IV (Csf1) Type VI (Cas13) Fighting Back: Anti-CRISPR Genes in Phage The CRISPR adaptive...creating a double-strand break. This method displays higher efficiency than HDR-mediated editing. The first... Genome engineers continue to work to develop a highly specific, programmable platform well-suited for...MS, Tsai SQ, Nguyen NT, Zheng Z, Joung JK. 2016. High-fidelity CRISPR-Cas9 nucleases with no detectable...

...We provide high quality AAV preps from select plasmids in the repository. Browse the University of Florida...and T492V. AAV44.9 The AAV44.9 serotype displays highly efficient transduction and lateral spread in the...Arun Srivastava and cite Petrs-Silva, et al. 2009. High-efficiency transduction of the mouse retina by tyrosine-mutant...a mouse model of complete congenital stationary night blindness. Hum Mol Genet . Nov 1;24(21):6229-39....acknowledge Arun Srivastava and cite Ling, et al. 2016. High-Efficiency Transduction of Primary Human Hematopoietic...27759036 Other citations include: Ling, et al. 2016. High-Efficiency Transduction of Primary Human Hematopoietic...

... maintain this plasmid collection page, which highlights plasmids available that may be useful for COVID...called SARS-CoV-2. Scientists have been working at lightning speed to elucidate as much as they can about this... develop and optimize the method termed Specific High Sensitivity Enzymatic Reporter UnLOCKING (SHERLOCK... now available from Xiaokun Shu's lab. A rapid, highly sensitive and open-access SARS-CoV-2 detection ...Transcriptase plasmids from Andrew Ellington's lab. High-surety isothermal amplification and detection of...

...that it is responsive to specific stimuli, such as light. On a broader scale, the expanded genetic code can... . Browse Synthetase Plasmids The table below highlights plasmids that contain aminoacyl tRNA synthetase...TAG Edward Lemke Browse Strains The table below highlights bacteria strains that have been modified to enhance...Chatterjee Browse Target Plasmids The table below highlights plasmids that contain genes with modified codons...

...deletion-mutant rabies virus AAV1 Wickersham 100799 pAAV-TREtight-mTagBFP2-B19G AAV1 Wickersham Introduction to ...tracing virus that lacks the ability to infect neighboring cells. For example, G-deleted rabies lacks the...starter" cell, but new virus cannot spread to any neighboring cells. To enable monosynaptic spread of this ...newly formed viruses are unable to infect any neighboring cells. Thus, through complementation, G-deleted...released from the starter cell and free to infect neighboring cells via G-protein receptors, which are naturally...

...cancer cells. Adenoviruses have high transduction efficiency and direct high but transient transgene expression...clinical trials. These viruses have a relatively high transduction efficiency, though this infectivity... can be delivered to a host and generate virus (right), similar to how natural viruses can be delivered...stranded linear DNA >8 Kb 105 Dividing/Non-dividing No High γ-Retrovirus Stable RNA <8 Kb 80-130 Dividing Yes...

...for tetracycline transregulation. Dox binds with high affinity to tTA and rtTA; thus, dox can be used ...plasmids third-generation rtTA On Elledge 19407 pTREtight2 Promoter contains a modified TRE that is silent...silent in absence of binding tTA or rTA; has high copy E. coli origin None Either Ralser 64238 pTet-IRES-EGFP...Expresses firefly luciferase hairpin and GFP under pTREtight promoter None Either Elledge 35625 pAAV-Ptet-RFP-shR-rtTA...promoter and enhancer options tTA Off Uney References Tight control of gene expression in mammalian cells by...

... Viral Vector Packaging Service Addgene produces high-quality viral vector preps from select plasmids ... and lentiviral vectors as in-stock preps. These high-titer preps are distributed as 100 µL solutions ...particles that are suitable for in vivo use. Order a high-quality and ready-to-ship AAV or lentivirus prep...services to you, we must ensure that you have the right to use the original plasmid. Distribution of the...

...Gene/Insert PI Kits Kit name Type PI Description High Complexity Golden Gate Assembly Standards Kit Cloning...end-joining ligation fidelity and bias data to predict high accuracy junction sets for Golden Gate assembly.... biological materials and intellectual property rights, and provides liability protection for material...qualified for-profit entities. Addgene reserves the right to determine if an entity is qualified to receive...

...types and/or developmental timeframes. Here we highlight some of the plasmids and tools available at Addgene...Plasmid Tools for Use in Worms The table below highlights plasmids that contain tools for use in worm research...

...optimized for each specific virus in order to generate high quality preparations. After production, all virus...titers obtained using ddPCR are generally 2–3 fold higher than those achieved using the standard qPCR method...digital PCR (ddPCR). Quality Control Addgene ensures high quality viral vectors by optimizing and standardizing...staining or SYPRO Ruby staining and the molecular weight and relative intensity of the viral capsid proteins...

...mutations are known to cause Rett syndrome, however, eight recurrent missense and nonsense mutations (R106W... labs Plasmids MECP2 Plasmids The table below highlights plasmids that express or target the MECP2 gene... 55–67. PMID: 30536762 Shahbazian et al. 2002. Insight into Rett syndrome: MeCP2 levels display tissue...

...that wildtype Cas9 with optimized chimeric gRNA has high efficiency but has been shown to have off-target...prefers a 'G' at the transcription start site to have high expression, so adding this G could help with expression... culture unless the targeting efficiency is very high. How should I design experiments for multiplexed...but the efficiency will be lower, although this might still be okay if you are introducing a selection...

...will have the highest specificity. To increase specificity, you can also consider using a high-fidelity Cas...gRNA targeting 5′ exon or essential protein domains High-fidelity Cas enzymes increase specificity. Dual-...a mammalian cell line that can be transfected at high efficiency Lentiviral transduction Cas9 and gRNA...

...vectors with the highest specificity and brightness, assessed using ssRNAseq or high-resolution imaging...screened thousands of enhancers for specificity and brightness of fluorescent labeling across brain regions ...

...the lowest biosafety protection levels. High quality and high titer in-stock AAV preps are affordable,... density centrifugation. Addgene’s comprehensive high-quality quality control process includes titering...

...combination allows IPI to generate antibodies to highly conserved proteins and other difficult-to-target...part of that mission, IPI is providing access to high-quality, well-characterized antibodies in an open...) Glypican Antibodies Glypicans are a family of highly conserved proteoglycans crucial for regulating ...

...luminescent opsins: fusion proteins of a light-emitting luciferase and a light-sensing optogenetic element, making...oxidizes its substrate (luciferin), it generates light that activates the opsin to either excite or inhibit...

...We provide high quality AAV preps from select plasmids in the repository. Browse our AAV collection of... Service: Recombinases Cre AAV Dre AAV Flpo AAV Light-Inducible Recombinases VCre AAV Browse the following...Cepko 183412 pAAV-CAG-FlpO CAG none 1, rg* Janelia Light-Inducible Recombinases ID Name Promoter Fluorophore...

...joining fragment comprising a κ light chain constant domain (C L ) and the κ light chain polyA tail sequences... for light chain expression, and an ER signal/leader sequence (L) for translocation of the light chain...in a new window) Durocher Y, et al. 2002. High-level and high-throughput recombinant protein production... R-mAbs are secreted into the culture media. For high level expression we recommend using the HEK-2936E...Link opens in a new window) . The schematic to the right shows the separate elements of the R-mAb expression...expression plasmid involved in coexpression of light (green) and heavy (blue) chains as driven by two CMV promoters...