Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 87 results for: otos

Showing: 21 - 40 of 87 results

  1. Plan Your Experiment

    Type
    Collection
    ...review the protocol associated with the specific plasmid in question (see CRISPR protocols from Addgene...10.1038/s41587-020-0490-7 PMID: 29305085 Hashimoto, M., & Takemoto, T. (2015). Electroporation enables the...used as positive controls when working out a new protocol. Read more about how to design your gRNA . Synthesize...editing experiments in mammalian cells. Nature Protocols , 17 (11), 2431–2468. https://doi.org/10.1038/...

  2. Neurodegeneration Research Collection

    Type
    Collection
    ...upper and lower motor neurons in the brain and spinal cord. Over time, a loss of motor neurons leads to...aberrant axon initial segment (AIS) plasticity with a motor neuron-specific reporter and a PAX7 inducible vector...expression of transcription factors for sensory, motor, and cortical neuron differentiation. Held et al...opens in a new window) Research workspace on Protocols.io (Link opens in a new window) . This resource...

  3. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...Phosphoprotein Synthesis Reagents References & Protocols Additional Resources Figure 1: SepRS adds a phosphoserine...SepRS/EF-Sep ( aka. pKD-SepRS-EFSep) References & Protocols Iterative Synthetically Phosphorylated Isomers....202110591. PMID 37477096 (Link opens in a new window) Protocols for using iSPI, rEcoli XPS, and pSerOTS-C1* (V70....2015.08.016. PMID 26365381 (Link opens in a new window) Protocol for using the Improved Reagents (ca. 2015) from....2012.08.031. PMID 22982858 (Link opens in a new window) Protocol for Reagents (ca. 2014) from the Rinehart lab:...

  4. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Near-Infrared Long Stokes Shift Photoactivatable Photoconvertible Photoswitchable Timers Self-Labeling Tags ...LSSmKate2-C1 - Mammalian Expression Return to top Photoactivatable (Off to On) Excitation and Emission wavelengths...HisB-PAiRFP2 - Bacterial Expression Return to top Photoconvertible (e.g. Green to Red) Protein Excitation (nm...Bacterial Expression Return to top Reversibly Photoswitchable (e.g. Off to On to Off) Protein Excitation ...

  5. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...Information Cloning Protocols Single guide cloning: Zhang Lab General Cloning Protocol (PDF, 237 KB) Dual...Dual guide cloning: Brzezinski Lab Dual Guide Protocol (PDF, 990 KB) Plasmid and Sequence Derivative Sources...Prdm1 and Vsx2 enhancers in the retina alters photoreceptor and bipolar cell fate specification, yet differs...

  6. Bacterial Expression Systems

    Type
    Collection
    ...collection of FPs, including photoactivatable, photoconvertible, and photoswitchable proteins, organized by ...Fusion Proteins — Making the Right Connection Photoactivatable Fluorescent Proteins Fluorescent Protein Timers...Inducible Promoters Repressible Promoters Synthetic Photobiology: Optogenetics for E. coli Return to top Reporter...

  7. TALEN Plasmids and Kits

    Type
    Collection
    ... 1000000030 Yamamoto Lab TALEN Construction and Evaluation Accessory Pack Takashi Yamamoto This accessory...Golden Gate. Validated in several species. Takashi Yamamoto Additional Plasmids for Use with Addgene TALEN... at the final assembly step in the Golden Gate protocol. Both plasmids 47388 and 47389 include a Flag ...

  8. Michael Davidson Fluorescent Protein Collection

    Type
    Collection
    ...Blue Cyan Green mEmerald Yellow Orange Red Photoactivatable The lab of Michael Davidson from Florida State...new window) for images, tutorials, microscopy protocols, and more. Empty Backbones Plasmid Description...Plasmid Description Industry Return to top Photoactivatable Plasmid Description Industry Return to top...

  9. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...infection. Dapeng Zhou Other Resources Protocols Bloom lab - Protocol and Reagents for Pseudotyping Lentiviral... Spike Protein Nemazee lab - Pseudotyped virus protocol for coronaviruses (DOCX, 184 KB) Addgene Blog ...

  10. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...basic-hFX-promotor(1kb) FXN hFXN Friedreich ataxia Michael Ristow 14979 pGL3-basic-hFX-promotor(1,3kb) FXN...basic-hFX-promotor(2,5kb) FXN hFXN Friedreich ataxia Michael Ristow 14981 pGL3-basic-hFX-promotor(2kb, distal... DCTN1 CMV ALS, motor neuron disease Trina Schroer 51221 CMV-CC2 DCTN1 CMV ALS, motor neuron disease Trina... ALS, motor neuron disease Caren Norden 105971 pME mKate2-dynactin1-811 DCTN1 mKate2 ALS, motor neuron...Aebischer 36154 pEGFP-p150Glued DCTN1 GFP CMV ALS, motor neuron disease David Stephens 36382 pSMP-DNMT1_1... Trina Schroer 51412 CMV-p150 DCTN1 CMV ALS, motor neuron disease Trina Schroer 51435 pGL3Basic_ME.1/ApoEpromoter...Charlet-Berguerand 74170 pVEX-CC1 DCTN1 tac ALS, motor neuron disease Trina Schroer 75437 p-AAV-sh[SNCA...

  11. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ...open-source' protocol for making customized zinc-finger arrays. Maeder ML et al. Nat Protoc. . 2009. Sept...customized zinc-finger nucleases. Foley JE et al. Nat Protoc. . 2009. Dec 3. 4(12):1855-1867. PubMed PMID 20010934...

  12. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ... a new window) . Protocols The Allen Institute for Cell Science has several protocol videos available ...Protein Guide Subcellular Localization Plasmids Protocols The Allen Institute for Cell Science has produced...

  13. AAV Packaged on Request

    Type
    Collection
    ... your AAV experiments, remember to share your protocols and results through Addgene’s Data Hub . By contributing...globe and shared our well-developed and trusted protocols and peer-reviewed techniques (Link opens in a ...preps. Data Hub Access Addgene’s Data Hub for protocols and results shared by other scientists using AAV...

  14. Fluorescent Proteins: FRET

    Type
    Collection
    ...excitation of the fluorophore and emission of a photon) of the donor can be measured using fluorescence... standards Thaler et al. (2005) Quantitative multiphoton spectral imaging and its use for measuring resonance...standards and fusion proteins Feldmann et al. (2023) Protocol for deriving proximity, affinity, and stoichiometry...

  15. Antibody Guide

    Type
    Collection
    ...applications, visit the Antibody section of the Addgene Protocols page. Antibody Applications - Quantification Methods...binding between the protein and DNA. Some ChIP protocols use enzyme digestion instead of sonication. This...Special considerations Many of the steps in this protocol vary depending on assay and microscopy techniques... may be a fluorophore or an enzyme. The basic protocols and controls follow that of IF (above). Sample...

  16. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR is ...–6 67,366 (Set A) 67,194 (Set B) Cas13a/C2c2 Protospacer flanking site (PFS) Library 79153 Knockout E....E. coli Zhang N/A N/A N/A - The protospacers contained in the library represent all 4096 (4 6 ) combinations...

  17. Antibodies

    Type
    Collection
    ...production process by sharing our protocols and quality control methods. Learn More Guide ...Recipient Instructions Addgene Help Center Antibody Protocols Looking for Antibody Plasmids? Browse our collection...

  18. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Type VI CRISPR...change in RNA. dPspCas13b does not require a Protospacer Flanking Sequence (PFS), making it a very flexible...
Showing: 21 - 40 of 87 results