Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 63 results for: rest

Showing: 21 - 40 of 63 results

  1. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...promoter or regulatory regions of your gene of interest. If the plasmid that you choose does not also ...transcription. Design your gRNA to target your gene of interest’s promoter/enhancer or the beginning of the coding...specific promoter or enhancer for your gene of interest. Available modifications include histone acetylation...

  2. Lentivirus Plasmids

    Type
    Collection
    ...plasmid — contains transgene, sgRNA, or shRNA of interest flanked by LTRs Packaging plasmid — contains packaging... Any expression cassette (promoter and gene of interest) can be cloned into the plasmid. Garry Nolan 12257...Bicistronic expression of EGFP and the gene of interest. Can also express shRNA (cloning H1-shRNA cassettes...

  3. CRISPR Plasmids - Tagging

    Type
    Collection
    ...plasmids for endogenous tagging of your gene of interest.... design. How to use CRISPR to tag your gene of interest Mendenhall and Myers Tagging System The Eric Mendenhall...plasmids as listed in each row. If your own gene of interest is currently unavailable, you will need to design...

  4. CRISPR Guide

    Type
    Collection
    ...conditions: The sequence is unique compared to the rest of the genome The target is present immediately ...Publications CRISPR Overview Bacteria have an interesting adaptive immune response that detects and eliminates... Cas enzyme and a gRNA specific to the gene of interest. The genomic target of the gRNA can be any ~20...activity with endogenous cell-type specific miRNAs to restrict CRISPR editing only to the target tissue. Browse... molecules associated with a genomic region of interest in vivo. Figure 12: Overview of purifying genomic...

  5. Fluorescent Protein Guide

    Type
    Collection
    ...localization and/or function of your protein of interest by fusing it to a fluorescent protein. FRET Find...Subcellular Localization Determine where your protein of interest resides by using a well-characterized fluorescent...

  6. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ... molecules associated with a genomic region of interest in vivo is essential to understanding locus function... molecules associated with a genomic region of interest in vivo. Figure 1: Overview of purification of...

  7. TALEN Engineering

    Type
    Collection
    ...repeat arrays of any desired length: the REAL (Restriction Enzyme And Ligation) assembly method (Sander ...Targeter enables users to search their sequences of interest for potential TALEN or TALE transcriptional activator...

  8. AAV for Neuronal Tracing

    Type
    Collection
    ...the system is to infect the cell population of interest with a deletion-mutant tracing virus that lacks..., Young JA, Callaway EM. 2007b. Monosynaptic restriction of transsynaptic tracing from single, genetically...

  9. CRISPR Plasmids - Yeast

    Type
    Collection
    ...promoter or regulatory regions of your gene of interest. If the plasmid that you choose does not also ...transcription. Design your gRNA to target your gene of interest’s promoter/enhancer or the beginning of the coding...

  10. Viral Vector Guides and Plasmids

    Type
    Collection
    ...plasmids for a wide array of research purposes. Interested in Addgene's viral services? See Addgene's Packaged...these viral vectors are used to transduce cells of interest. Specific modifications to these plasmids, such...

  11. Microbiology Resources

    Type
    Collection
    ...microbiology resources: Find plasmids for your microbe of interest, including bacteria, viruses, protozoa, fungi,... cerevisiae - Dueber Lab Yeast Prototrophy : Restoration of prototrophy in common S. cerevisiae lab strains...

  12. Promega Plasmid Collection

    Type
    Collection
    ... viral biology without disturbing the virus of interest and monitoring endogenous protein dynamics in ...interaction (PPI) studies, where each protein of interest is fused to either LgBiT or SmBiT and expressed...

  13. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...promoter or regulatory regions of your gene of interest. If the plasmid that you choose does not also ...transcription. Design your gRNA to target your gene of interest’s promoter/enhancer or the beginning of the coding...

  14. Zebrafish Plasmid Collection

    Type
    Collection
    ...for plasmids containing your zebrafish gene of interest? Search Addgene's collection for plasmids that...vertebrate brain - Alex Schier Lab Other Tools of Interest A few examples of popular tools to use in zebrafish...

  15. CRISPR Plasmids - Plants

    Type
    Collection
    ...promoter or regulatory regions of your gene of interest. If the plasmid that you choose does not also ...transcription. Design your gRNA to target your gene of interest’s promoter/enhancer or the beginning of the coding...

  16. Rett Syndrome

    Type
    Collection
    ...regardless of age or symptom severity, suggesting that restoring MECP2 expression in humans could be curative. ...therapeutic strategies to treat Rett syndrome and restore functional MECP2 in cells. Search (Link opens in...

  17. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...cells that are then screened for a phenotype of interest. Screening experiments using a pooled CRISPR library...individual library page. Find Pooled Libraries of Interest Click on different properties to create a custom...

  18. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Constitutive or Cre-dependent) Cell-specific restoration of stimulus preference after monocular deprivation...augmented transmembrane chloride movement by gerbil prestin (SLC26a5). PLoS One. 2014 Jun 5;9(6):e99095. Joseph...

  20. Antibodies

    Type
    Collection
    ...research applications. Browse Antibodies Production Interested in our production process? At Addgene, we provide...
Showing: 21 - 40 of 63 results