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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 90 results for: tat

Showing: 21 - 40 of 90 results

  1. TALEN Plasmids and Kits

    Type
    Collection
    ...TALEN Guide. These collections and accompanying documentation allow one to efficiently assemble TALEN constructs...truncated at both the N and C terminus and induces mutation at rates much higher than the parental vectors...generation architecture has been shown to increase mutation induction when using TALENs. The FokI domains ...homodimeric or enhanced heterodimeric (ELD, KKR mutations) FokI domains. 40131 pCAG-T7-TALEN(Sangamo)-FokI-KKR-Destination...are designed to target and edit the epigenome of putative enhancers in mammalian cells. These plasmids allow...suitable for the TALEN-based generation of germline mutations in Bombyx mori and Drosophila melanogaster . Zhang...

  2. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...or not, without a guaranteed titer. Any titer expectations communicated by email will supersede the general...after you submit your request, so we can set expectations to the best of our knowledge from the start....not, with a longer turnaround time. Any timing expectations communicated by email will supersede the general...material received. Shipping While Addgene assists importation by including thorough shipping documents and ...taxes, and to complete any additional customs documentation that might be required by your country for the...

  3. Zhang Lab CRISPR Page

    Type
    Collection
    ...loss-of-function mutations in p53 and Lkb1 , as well as homology directed repair-mediated Kras G12D mutations, leading...harnessed the type II CRISPR nuclease system to facilitate genome editing in mammalian cells (Cong et al...AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei. Return to top...KRAS , p53 and LKB1 , the top three significantly mutated genes in lung adenocarcinoma. Delivery of a single...hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR This plasmid facilitates Cre/loxP recombination and fluorescence assisted...

  4. Plasmids for Stem Cell Research

    Type
    Collection
    ...continuously divide while maintaining an undifferentiated state. Stem cells are classified as pluripotent or multipotent...adult somatic cells are returned to a pluripotent state using a cocktail of factors (Oct3/4, Sox2, c-Myc...This “reprogramming” of the cell to an embryonic state holds immense potential for drug development, disease... cell division, all of which lower the risk of mutation and minimize tumorigenicity, an inherent disadvantage...Lentiviral Mouse Direct Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts. Cell...Neurons Lentiviral Human Rapid Ngn2-induction of excitatory neurons from hiPSC-derived neural progenitor ...

  5. COVID-19 Resources

    Type
    Collection
    ...Binding Domain Deep Mutation Scanning Library - Library of Spike protein RBD mutations for comprehensive...Libraries : Pooled libraries of spike protein mutations. Research Articles : A list of free access articles...cleaves precursor proteins to a biologically active state. The SARS-CoV-2 S protein contains a potential cleavage...Libraries - Libraries of variant Spike protein RBD mutations for comprehensive mapping (Jesse Bloom, Tyler ...

  6. Fluorescent Protein Guide: In Vivo Imaging

    Type
    Collection
    ... iRFP has resulted in a split fluorescence complementation probe used for detecting protein-protein interactions... Plasmids for In Vivo Imaging Far Red Protein Excitation/Emission Brightness Find Plasmids mCardinal 604...28.98 pEF.myc.ER-E2-Crimson Near-Infrared Protein Excitation/Emission Brightness Find Plasmids iRFP670 643...5.3 pPAS-E and pK-GAFm Photoactivatable Protein Excitation/Emission Brightness Find Plasmids PAiRFP1 690...

  7. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...glyoxysomal, mitochondrial, peroxisomal) Mutation (specific mutation, "active site", "active site loop", or...shared experience for the research community and facilitates scientific collaborations between institutions...interface") ID Plasmid Description Gene/Insert Mutations Additional Resources MDH CUREs Community (Link...

  8. AAVED

    Type
    Collection
    ...minute presentation, followed by 20 minutes of questions/discussion. The goal of the presentation is to...Addgene will also be speaking or leading workshops. Tentative Meeting Agenda Thursday, September 20 6:00 PM-...how to best help contribute to this publication. Citation information for openly shared communication: Adeno-Associated...

  9. CRISPR Plasmids - Bacteria

    Type
    Collection
    ... create A->G (or T->C on the opposite strand) mutations. ID Plasmid Promoter PI Publication Nick CRISPR...RNA sequence, they adopt an enzymatically active state and can bind and cleave additional RNAs regardless...enCHIP system uses an anti-FLAG antibody to immunoprecipitate FLAG-tagged Cas9. Design your gRNA sequence... DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP) using CRISPR. Biochem Biophys Res...

  10. Zebrafish Plasmid Collection

    Type
    Collection
    ...of enhancers and non-coding RNAs in zebrafish - Tatjana Sauka-Spengler Lab Lineage Tracing Cell lineage... typically relies on genetic markers, somatic mutations, or labeling with fluorescent proteins to visualize...dynamics in vivo. Bimolecular fluorescence complementation (BiFC) tools - Marco Morsch Lab. A vertebrate...potential cancer drivers in vivo. Biotagging toolkit - Tatjana Sauka-Spengler Lab. A genetic binary system for...

  11. Genetic Code Expansion

    Type
    Collection
    ...endogenous TAG codons mutated to TAA, RF1 (prfA) deleted, fabR spontaneously mutated, serB deleted for phosphoserine...jannaschii , M. barkeri , or E.coli and can be mutated and screened through directed evolution to charge...RF1 function removed, MutS restored so decreased mutation rate George Church 68306 C321.ΔA all TAG sites... to UAA. mutS+, λ-, ΔybhB-bioAB, ΔprfA, ΔserB mutations for improved yield and growth Jesse Rinehart 197655...

  12. CRISPR References and Information

    Type
    Collection
    ...CRISPResso CRISPResso2 is a robust and easy-to-use computational pipeline for the analysis of genome editing ...experiments. It enables rapid and intuitive interpretation of results produced by amplicon sequencing.... opens in a new window) A tool that identifies putative target sites for S. pyogenes Cas9, S. aureus Cas9...off-targets, target's genomic location, and genome annotation. Available genomes are human (hg19 & hg38), mouse...

  13. Cancer Research Plasmids and Resources

    Type
    Collection
    ...for transitioning between anabolic and catabolic states. Ras Ras is a GTPase that is essential for regulating...growth of the cell. p53 p53, the most frequently mutated gene in human cancer, is a transcription factor...labs for characterization of all possible p53 mutations. Resources New Tool for Lineage Tracing: The ClonTracer...

  14. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...installing targeted insertions, deletions, and point mutations using a prime editing guide RNA (pegRNA). As with...tools are available: PE2 - introduction of five mutations in RT enzyme PE3 - PE2 plus additional sgRNA PE4...additional nuclear localization signals, and two mutations in Cas9 to enhance nuclease activity; can be used...

  15. AAV Packaged on Request

    Type
    Collection
    ...steps: MTA facilitation for the transfer plasmid, including legal approval and an implementation letter DNA...pricing for viral vector preps, which includes MTA facilitation, DNA amplification, and high-quality quality...

  16. Plant Plasmids and Resources

    Type
    Collection
    ...(Link opens in a new window) for Targeted Gene Mutation in Rice - Yinong Yang. The MoClo CRISPR/Cas Toolkit...metabolic engineering in plants (including tobacco and potato) - Scott Lenaghan. Joint Modular Cloning (JMC) ...resource composed by a collection of databases, computational and experimental resources that relate to the...

  17. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ... create A->G (or T->C on the opposite strand) mutations. CRISPR-X, a base-editing system from the Bassik...recruit a cytidine deaminase and induce somatic hypermutation in a 100 bp window. This technique creates diverse...enCHIP system uses an anti-FLAG antibody to immunoprecipitate FLAG-tagged Cas9. In the CAPTURE system, Cas9...

  18. Ras Pathway

    Type
    Collection
    ...GTPases have an active (GTP) and inactive (GDP) state, regulated by the addition and removal of a phosphate...regulating the growth of the cell, however when mutations occur in the Ras gene it can be stuck in a constitutively...PubMed PMID: 17428555 . RAS signaling pathways, mutations and their role in colorectal cancer. Zenonos K...

  19. Neurodegeneration Research Collection

    Type
    Collection
    ...dominant manner, are associated with known genetic mutations in one of the following genes: SOD1, C9ORF72, ... huntingtin (HTT) gene. An individual with the mutated HTT gene will have huntingtin proteins with abnormally...-onset forms, a fraction of these are due to a mutation in one of three different inherited genes: Presenilin...

  20. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...precise gene editing. These edits can include mutations seen in human patients, protein tags, or loxP/...off-target activity due to rationally designed mutations. Researchers have expanded the CRISPR field beyond... DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP) using CRISPR. Biochem Biophys Res...
Showing: 21 - 40 of 90 results