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We narrowed to 123 results for: tra

Showing: 21 - 40 of 123 results

  1. Plasmids for Stem Cell Research

    Type
    Collection
    ...reprogramming factors Efficient germ-line transmission obtained with transgene-free induced pluripotent stem cells...Reprogramming Plasmids Differentiation and Transdifferentiation Plasmids Stem Cell Research Plasmids Stem...methods with the goal of helping readers choose a strategy suited to their research. Once you know which ...differentiated somatic cell into another via transdifferentiation. This method of cell lineage conversion ...technology. Browse Addgene's collection of Transdifferentiation Plasmids below. Many of the techniques for... for reprogramming, differentiation, and transdifferentiation utilize plasmids to increase expression ...human Klf4, Oct4, c-Myc, and Sox2 as VP-16 transcriptional activating fusions in single lentiviral vectors...

  2. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...Rinehart lab developed a new strain of recoded E.coli , rEcoli XpS (Bacterial strain #192872) , and an optimized... protein. Using the recoded strain of E. coli , C321.ΔA (Bacterial strain #68306) , with the optimized...phosphoserine orthogonal translation system, SepOTSλ (Plasmid #68292) , they were able to demonstrate unambiguous ...pLtetO MBP-MEK1 XX Amp) Strain 52055 EcAR7 Plasmid 52054 SepOTSµ ( aka. B40 OTS) Strain 34929 BL21ΔserB Plasmid...phosphorylation is one of the most abundant forms of posttranslational modification in cells and research into its...Lajoie et al., 2013) and improving the orthogonal translation system to make phosphorylated proteins with one...optimized phosphoserine orthogonal translation system, pSerOTS-C1* (V70) (Plasmid #188537) . These reagents...

  3. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...introduce gRNA-targeted single-strand breaks in DNA instead of the double-strand breaks created by wild type... opposite strands of your DNA in close proximity. These double nicks create a double-strand break (DSB...dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. ... BspQI Transfection Puromycin yes, cut Ji-Long Liu 45946 pU6-BbsI-chiRNA dU6 BbsI Transfection none, need...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...functional CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location based on a gRNA-defined...non-homologous end joining (NHEJ). Double nicking strategies reduce unwanted off-target effects. Nickase mutants...

  4. Viral Vector Guides and Plasmids

    Type
    Collection
    ...packaging capacity, high transduction efficiency, and high but transient transgene expression.They can induce...130 nm Dividing Yes Moderate AAV Transient or Stable* Single-stranded linear DNA ∼4.5 kb 18–26 nm Dividing.../Non-dividing No* Very Low Adenoviral Transient Double-stranded linear DNA 5–36 kb 105 nm Dividing/Non-dividing...of viral vectors and find viral production and transfer plasmids for a wide array of research purposes...are versatile tools because of their ability to transduce dividing and non-dividing cells. These virusal...immunogencity. These viral vectors can direct long-term transgene expression, but generally don't integrate into...just retroviral vectors, have a relatively high transduction efficiency and only infect dividing cells. They...

  5. Plant Plasmids and Resources

    Type
    Collection
    ...Plant transcription factors for Agrobacterium-mediated transient expression or plant transformation - Silin...parts for assembling plant transformation vectors - Daniel Voytas. Transcription Factors The following collections...expression in plants: Maize Transcription Factor ORFeome - John Gray. Rice Transcription Factor ORFeome - John...Plant Base Editors Save Time with Transient Plant Leaf Transformations RNA Interference in Plant Biology...Figshare - Plant Illustrations (Link opens in a new window) - Collection of illustrations and pictures of...Plant Plasmids and Resources CRISPR Cloning Transcription Factors RNAi Empty Backbones Fluorescent Proteins...Engineered biocontainable RNA virus vectors for non-transgenic genome editing across crop species and genotypes...

  6. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...introduce gRNA-targeted single-strand breaks in DNA instead of the double-strand breaks created by wild type... opposite strands of your DNA in close proximity. These double nicks create a double-strand break (DSB...dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. ...functional CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location based on a gRNA-defined...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...base editors convert C->T (or G->A on the opposite strand) within a small editing window specified by the...guanosine to create A->G (or T->C on the opposite strand) mutations. ID Plasmid Promoter PI Publication ...

  7. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...sites are on separate DNA molecules (in trans), a translocation event is generated between the target sites...can also be useful for lineage tracing, evaluating infection/transfection efficiency, selecting desired...mycobacteriophage, are serine SSRs that cleave all four DNA strands at once. They can mediate the integration of a ...VCre, and Vika. These systems work by sequential strand exchange at a specific target site (called loxP...after recombination (inversion, deletion, or translocation) depends on the orientation of each target site... If the target sites (loxP) are on the same DNA strand in opposite directions, recombination results in...reversed. B) If the target sites are on the same DNA strand (in cis) in the same direction, the sequence between...

  8. Retrovirus Plasmids

    Type
    Collection
    ...into multiple plasmids for safety: Transfer plasmid — contains transgene, sgRNA, or shRNA of interest flanked... the transgene fused to eGFP. Hans Clevers 18760 MSCV IRES Luciferase MSCV Plasmid for transgene expression...and transfer plasmids. Viral... Viral Plasmids Gamma-Retroviral Plasmids Gamma-Retroviral Plasmids Transfer Plasmids...encoded on RNA. The genome includes a reverse transcriptase and an integrase to insert the viral genome ...can take advantage of this to stably integrate transgenes of interest into a variety of cell types. Wild-type...collection highlights popular gamma-retroviral transfer, packaging, and envelope plasmids. Read our Gamma-retroviral...

  9. Zebrafish Plasmid Collection

    Type
    Collection
    ...- Tatjana Sauka-Spengler Lab Lineage Tracing Cell lineage tracing in multicellular systems typically relies...Lewis Lab PhOTO Zebrafish: A Transgenic Resource for In Vivo Lineage Tracing during Development and Regeneration...Periklis Pantazis Lab Multibow: digital spectral barcodes for cell tracing - Sean Megason Lab Zebrabow - Albert... (~3 months), ease of care and breeding, and transparency of the embryo and larvae, it is easy to culture...standard toolbox of genetic methods, such as Tol2 transposons, Cre-mediated site-specific recombination, morpholinos...emerged as a model system for human disease and translational research, including the study of cancer, inflammation...-Cas9 system - Shuo Lin Lab Re-purposed Ac/Ds transgenic system enabling CRISPR/dCas9-interference (CRISPRi...

  10. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...introduce gRNA-targeted single-strand breaks in DNA instead of the double-strand breaks created by wild type... opposite strands of your DNA in close proximity. These double nicks create a double-strand break (DSB...dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. ...functional CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location based on a gRNA-defined...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...Base editors convert C->T (or G->A on the opposite strand) within a small editing window specified by the...guanosine to create A->G (or T->C on the opposite strand) mutations. CRISPR-X, a base-editing system from...

  11. Antibody Production

    Type
    Collection
    ...diluting or concentrating to a final concentration of 0.9–1.1 mg/mL. Antibody concentration is confirmed...Blotting (WB) Proteins are extracted from cells, separated by SDS-PAGE, transferred to a membrane, and labeled...of co-purified plasmid from transfection. This residual plasmid is extracted and amplified with primers...expression vectors, human embryonic kidney cells are transfected with the plasmid(s) encoding the heavy and light... the buffer can be removed by dialysis or gel filtration. Titer and Purity Antibodies are initially titered...serial dilutions of a standard antibody of known concentration and stained with Coomassie blue. Protein band...standard curve is generated. The final antibody concentration is calculated from the standard curve. Using...

  12. CRISPR Plasmids - Yeast

    Type
    Collection
    ...introduce gRNA-targeted single-strand breaks in DNA instead of the double-strand breaks created by wild type... opposite strands of your DNA in close proximity. These double nicks create a double-strand break (DSB...dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. ...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...functional CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location based on a gRNA-defined...base editors convert C->T (or G->A on the opposite strand) within a small editing window specified by the...guanosine to create A->G (or T->C on the opposite strand) mutations. ID Plasmid Gene/Insert Promoter Selectable...

  13. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...red over time, allowing tracking of processes such as intracellular trafficking or cell cycle dynamics....are monomers at low concentration but are prone to dimerization at high concentration. Proteins that are...eqFP611 559 611 35 Tetramer eqFP611-N1 - Mammalian Expression DsRed2 563 582 24 Tetramer pCAG-DsRed2 - Mammalian... - Mammalian Expression mStrawberry 574 596 26 4.5 50 min Monomer mStrawberry-N1 - Mammalian Expression...contain the A206K mutation are monomeric at all concentrations, so this is mutation is noted when present....CYPet-C1 - Mammalian Expression AmCyan1 453 486 11 Tetramer AmCyan1-N1 - Mammalian Expression mTFP1 462 492...Expression (cysteine-free SGFP2) ZsGreen 493 505 39 Tetramer pHIV-Zsgreen - Mammalian Expression (this is a...

  14. CRISPR Plasmids - Plants

    Type
    Collection
    ...introduce gRNA-targeted single-strand breaks in DNA instead of the double-strand breaks created by wild type... opposite strands of your DNA in close proximity. These double nicks create a double-strand break (DSB...dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. ...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...functional CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location based on a gRNA-defined...base editors convert C->T (or G->A on the opposite strand) within a small editing window specified by the...guanosine to create A->G (or T->C on the opposite strand) mutations. ID Plasmid Gene/Insert Promoter Selectable...

  15. Viral Production

    Type
    Collection
    ...of Pennsylvania Vector Core. Transfections are performed using the transfer plasmid, a plasmid encoding...are purified by iodixanol gradient ultracentrifugation and concentrated to purity and titers adequate for...cell cultures are inspected for sterility. Transducibility Some viral vectors are tested in vitro and ...section for images). Figure 2: AAV Pro cells were transduced with either pAAV-Ef1a-mCherry-IRES-Cre (55634...cleared by low-speed centrifugation and then by filtration through a 0.45 µm polyethersulfone (PES) membrane... been stored at -80 °C and thawed. Lentivirus titration is performed using a 293T cell line in the presence...using a lentiviral droplet digital PCR (ddPCR) titration protocol (modified from Wang et al. (2018) (Link...

  16. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...information on substrates and synthases needed for use of these tags. Plasmids Addgene ID Plasmid Substrate Feature...simultaneously with dual labeling when used with two substrates Can be used in live or fixed cells, and for internal... proteins and use enzymatic binding to attach substrate Control plasmids with a recognizable sub-cellular... is based on the human O6-alkylguanine-DNA-alkyltransferase (hAGT), a DNA repair protein, and is suitable...protein tags based on the acyl carrier protein. Substrates are derivatives of Coenzyme A (CoA). These tags...Vector SNAP-tag Empty backbone for stable and transient mammalian expression 101133 pSNAP-CaaX Control...Vector CLIP-tag Empty backbone for stable and transient mammalian expression 101134 pCLIPf-H2B Control...

  17. Zhang Lab CRISPR Page

    Type
    Collection
    ... of the CRISPR RNA (crRNA) and the trans-activating crRNA (tracrRNA; Hsu et al ., Nature Biotechnology...plasmids #61422 - #61424 were designed for transient transfection : 61422 : Expresses dCAS9-VP64 activator... cassettes to express the CRISPR RNA array and tracrRNA separately (Cong et al ., Science 2013). Additionally...or SpCas9n, D10A nickase) + CRISPR RNA array + tracrRNA: This plasmid contains three expression cassettes...human codon-optimized SpCas9; sgRNA can be co-transfected as PCR amplicons (Ran et al., Nature Protocols...references are below. Return to top Cas9 SAM transcriptional activation plasmids and screening library CRISPR...) is an engineered protein complex for the transcriptional activation of endogenous genes. It consists...

  18. Fluorescent Proteins: FRET

    Type
    Collection
    ...Resonance Energy Transfer (FRET) is a process by which energy is non-radiatively transferred from an excited...Quantitative multiphoton spectral imaging and its use for measuring resonance energy transfer mCerulean, mVenus... energy transfer to multiple acceptors Koushik et al. (2009) Anomalous surplus energy transfer observed...fluorescent proteins, and other molecules have been demonstrated for use in FRET experiments. FRET efficiency...FLIM) or FLIM-FRET, where the increased energy transfer through FRET reduces the donor lifetime. FRET ...fused to the same protein (also referred to as intramolecular or unimolecular FRET). Learn more about FRET... of the donor emission and acceptor excitation spectra (×10 15 M -1 cm -1 nm 4 ). Values obtained from...

  19. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...easily and inexpensively generate R-mAbs by transiently transfecting the plasmids into mammalian tissue culture... Anti-Pan-Neurofascin (extracellular) [A12/18R] Pan-Neurofascin (extracellular) Rat Mouse IgG2a 177439...Anti-Kv1.4 K+ channel (extracellular) [L71/5R] Kv1.4 K+ channel (extracellular) Rat Mouse IgG2a 177489...Mouse IgG2a 177564 Anti-ADAM22 (extracellular) [N57/2R] ADAM22 (extracellular) Mouse Mouse IgG2a 177565 Anti-GluN2B...channel Rat Mouse IgG2a 188161 Anti-TRAAK K+ channel [N445/27R] TRAAK K+ channel Mouse Mouse IgG2a 188162...Anti-Kv1.4 K+ channel (extracellular) [L71/22] Kv1.4 K+ channel (extracellular) Rat Mouse IgG2a 190292...N382/14] MFF Human Mouse IgG2a 190320 Anti-TRAK2 [N390/43] TRAK2 Human Mouse IgG2a 190321 Anti-Navbeta2 Na...

  20. Antibody Guide

    Type
    Collection
    ...samples to extract proteins. Homogenize (tissues only), lyse, and then collect protein extract by centrifugation...hybridoma cells into plasmids. Plasmids are then transfected into cells and induced to express antibodies....to lot, as immune responses have high inter- and intra-individual variability. Other Affinity Reagents ...and therefore should be kept at 4 °C. Record and track lot numbers for all antibodies used in the lab. ...reaction in the presence of several different substrates. The luminescence produced by the reaction can... an SDS-PAGE gel to separate proteins by size. Transfer proteins from the gel to a membrane. Incubate ...and block the membrane. Activate the HRP with a substrate kit. Image the membrane using either X-ray film...
Showing: 21 - 40 of 123 results