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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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In Category

We narrowed to 84 results for: Mpl;

Showing: 41 - 60 of 84 results

  1. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...interacts only with their own target sites (for example, Cre with lox sites, Flp with FRT sites, Dre with...preferred codon usage will improve expression. For example, iCre or FLPo in mammalian cells may facilitate...translocation of a gene of interest. Using Cre or Flp as examples, here are a few popular recombinase-dependent ...

  2. Rett Syndrome

    Type
    Collection
    ...methylated DNA and the recruitment of the NCoR complex for proper nervous system function. While there...expressing Cas9 and empty gRNA backbones. A few examples of additional CRISPR resources can be found below...cell line repository (Link opens in a new window) Sampled - one of the world's largest biorepositories (Link...

  3. Bacterial Expression Systems

    Type
    Collection
    ...transfer (FRET) and bimolecular fluorescence complementation (BiFC) are techniques that have been widely...compatible vectors for bimolecular fluorescence complementation experiments in plants. The Zinc Finger Consortium...Introduction to FRET Bimolecular Fluorescence Complementation Makes Visualizing Protein–Protein Interactions...

  4. Plasmids for Stem Cell Research

    Type
    Collection
    ...generate iPSCs. The generation of iPSCs is relatively simple in concept: ectopically express a cocktail of stem...and Xeno-Free Culture Conditions: A Clinically Compliant Approach. Stem Cells Transl Med. 2015 Mar 5. pii...Lentiviral Mouse Direct Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts. Cell...

  5. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...using a pooled CRISPR library are far more complex than using CRISPR to modify a single genomic locus...screening experiments require electroporation to amplify the pooled library and the use of Next-Generation...3rd 6 12,727 Yeast Inducible CRISPRi Library – Ampl133 161829 Inhibition Yeast Tavazoie N/A 6-12 51,681...

  7. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...possibly resulting in frameshifts. If a repair template with high homology to the DNA surrounding the ...Cas9 species = S. pyogenes (PAM = NGG) 51306 pUC57-Simple-gRNA backbone T7 BsaI In vitro transcription none...

  8. AAV Viral Preps

    Type
    Collection
    ...Rabies Virus-Based Serotype Serotype Testing Samples Small samples of control AAV vectors for trial purposes...

  9. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...specific genomic changes, researchers use DNA repair templates with homology to the DNA flanking the DSB and ...edit close to the gRNA PAM site. When a repair template is present, the cell may repair a DSB using homology-directed...

  10. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and ...edit close to the gRNA PAM site. When a repair template is present, the cell may repair a DSB using homology-directed...

  11. CRISPR Plasmids - Parasites

    Type
    Collection
    ...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and ...edit close to the gRNA PAM site. When a repair template is present, the cell may repair a DSB using homology-directed...

  13. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...reducing the time to experiments by removing the amplification and extraction steps required when one receives...through our website. Every order will need to be completed independently including the check out and will...

  14. Optogenetics Plasmid Collection

    Type
    Collection
    ...plasmids for neuronal excitation and inhibition. For example, search for "ChR mCherry", "Cre inhibition", or...to control a protein or cellular activity. For example, search "Cdc42", "transcription", or other terms...

  15. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and ...edit close to the gRNA PAM site. When a repair template is present, the cell may repair a DSB using homology-directed...

  16. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ... sequence verification, may not yet have been completed at the time of shipment. If any discrepancies ...for any additional customs fees or taxes, and to complete any additional customs documentation that might...

  17. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...the SARS-CoV-2 Spike protein and you will find examples of each in Addgene's collection: HIV-based lentiviral...and Packaging Plasmids Reporter Plasmids A few examples of reporter plasmids that can be used for measuring...

  18. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...allow for dual labeling and visualization of complementary protein function or localization. ACP and MCP...permitting selective simultaneous labeling in a sample. Please see the note below for information on substrates...

  19. Viral Vector Guides and Plasmids

    Type
    Collection
    ...and are suited for specific research goals. For example, AAV vectors are typically preferred for in vivo... self-inactivating, meaning that they can only complete one round of transcription of their viral genome...

  20. AAV Molecular Tools

    Type
    Collection
    ...transactivator (tTA) with a positive feedback loop for amplified tTA expression. 1 Viviana Gradinaru 99121 pAAV-ihSyn1...transactivator (tTA) with a positive feedback loop for amplified tTA expression. 1 Viviana Gradinaru 117383 TRE-DIO-eYFP...
Showing: 41 - 60 of 84 results