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Showing: 41 - 60 of 94 results

  1. Validated gRNA Sequences

    Type
    Collection
    ...for use in CRISPR experiments. This datatable is compiled from plasmids in Addgene's database as well as...designed for? A given gRNA sequence may only be compatible with a single species or PAM binding variant ...Which CRISPR application is this gRNA sequence compatible with? CRISPR knockout experiments use targeting...the Cas9 application the gRNA was designed to accomplish. Validated gRNA Sequence Datatable Target Gene...

  2. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...intravitreal injection in mice and marmosets as compared to the AAV2 serotype. The AAV2(Y444F) serotype...in the retina following subretinal injection as compared to the parental AAV44.9 serotype. It was developed...and restores visual function in a mouse model of complete congenital stationary night blindness. Hum Mol...

  3. CRISPR Plasmids - Tagging

    Type
    Collection
    ...This CRISPR/Cas plasmid-system consists of two components: A vector containing Cas9 and a validated gRNA...endogenous genes for the isolation of native protein complexes. This vector serves as a backbone to clone the... as homology arms. The system consists of two components: An all-in-one CRISPR/Cas9 vector based on the...

  4. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ...system, developed by the Zhang lab, which has comparable efficiency to the optimized chimeric design but...Where are double strand breaks (DSBs) induced, compared to where the target site sequence (protospacer...how closely homologous the off-target sites are compared to the on-target site, the specific site sequence...

  5. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...designed for? A given gRNA sequence may only be compatible with a single species or PAM binding variant ...Which CRISPR application is this gRNA sequence compatible with? CRISPR knockout experiments use targeting...

  6. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...knockout, repress, and activate gene targets. To accomplish this they have taken advantage of the Zhang lab...guide within a U6-guide cassette. This process is compatible with all pX458 family plasmids. Additional Cloning...

  7. Jaenisch Lab CRISPR Plasmids

    Type
    Collection
    ...RNA-guided transcriptional activator system with two components, the dCas9 activator protein and the single guide...provides the transactivation activity, the sgRNA component provides the specificity and is designed to target...

  8. Retrovirus Plasmids

    Type
    Collection
    ...γ-Retrovirus (gamma-retrovirus) is an RNA virus comprised of its genome and several structural and enzymatic...gets integrated into the host's genome by the accompanying integrase protein. When scientists discuss retrovirus...

  9. Antibody Plasmid Collection

    Type
    Collection
    ...recombinant antibodies, created by cloning antibody components into plasmids and expressing these in bacteria... and Evolving Functional Proteins. METHODS: A Companion to Methods in Enzymology 8, 94–103 (1995). Carlos...

  10. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...transcriptase. Engineering of the PE enzyme and related components have helped to improve the efficiency of editing...additional sgRNA PE4 - PE2 plus MLH1 (a protein component of MMR) mutant to inhibit MMR PE5 - PE3 plus MLH1...

  11. AAVED

    Type
    Collection
    ...different experimental goals. How does this method compare to other similar methods? Where does this method...For All Attendees: To help us best prepare and compile resources for this meeting's publication, we are...

  12. Arf GTPase Family

    Type
    Collection
    ...). A compatible GEF, GTPase, and GAP set is referred to as a minimal triad, with each component involved...

  13. Microbiology Resources

    Type
    Collection
    ...Ralstonia solanacearum species complex (Rssc) gene knockout and complementation constructs - Lahaye Lab Vaccinia...

  14. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...to target mTurquoise2 to various subcellular compartments pCEP4CyPet-MAMM Cyan Mammalian Expresses mammalian... Clover pGWF1 Cyan & Yellow Bacterial Gateway-compatible vector to express a gene of interest fused between...

  15. Rett Syndrome

    Type
    Collection
    ...and related MECP2 disorders. The RSRT has a comprehensive, strategic and aggressive plan to use gene editing...methylated DNA and the recruitment of the NCoR complex for proper nervous system function. While there...

  16. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...using a pooled CRISPR library are far more complex than using CRISPR to modify a single genomic locus... particular organism, and the library is only compatible with cells derived from that organism. All of...

  17. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Chris Garcia 157115 pD649-HAsp-TREM2-COMP5AP-AviTag-9xHis TREM2 COMP5AP, Avi, His CMV, SP6 Alzheimer's Chris...Chris Garcia 157447 pD649-HAsp-CSF1R-COMP5AP-AviTag-9xHis CSF1R COMP5AP, Avi, His CMV, SP6 Hereditary diffuse...Chris Garcia 157495 pD649-HAsp-ERBB4-COMP5AP-AviTag-9xHis ERBB4 COMP5AP, Avi, His CMV, SP6 ALS Chris Garcia...

  18. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...MultiSite Gateway recombination-based cloning of complex polycistronic lentiviruses. Can express Cas9 with... and plasmids that provide Cas9 variants. All components are then assembled into a single T-DNA binary...

  19. Synthetic Biology - Metabolism

    Type
    Collection
    ...biology plasmids related to metabolic pathways and components. Examples Include: Biofuels Natural Product Pathways...
Showing: 41 - 60 of 94 results