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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 123 results for: REP

Showing: 41 - 60 of 123 results

  1. Plan Your Experiment

    Type
    Collection
    ...genome editing. Scientific Reports , 5 , 11315. https://doi.org/10.1038/srep11315 PMID: 26066060 Zukher, ...experimentally verified Cut Nick Homology Directed Repair (HDR) Generate specific genomic edits, like point...fusions with proteins that promote HDR Requires a repair template Lower editing efficiency compared to NHEJ...pegRNA optimization can be challenging Prime Edit Repress or Interfere (CRISPRi) Reduce gene expression without...sequence dCas9 or dCas9 fusion with transcriptional repressor (such as dCas9-KRAB) dCas9-KRAB can be more effective...requires donor DNA to serve as template for the repair. Once you settle on your gRNA, you can design a...target in your samples to ensure there are no discrepancies with the reference sequence you used for gRNA...

  2. Brain Initiative Collection

    Type
    Collection
    ...Plasmid Description PI Viral Preps Addgene distributes ready-to-use viral preps made from plasmids in our... BRAIN Initiative collection of plasmids, viral preps, and antibodies here. Collections...the research community. Browse plasmids , viral preps , and antibodies created with support from the BRAIN...use. Learn more about our viral service ID Viral Prep Description PI 65417-AAV8 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine...plasmid encoding for Archon1 fluorescent voltage reporter, Cre-dependent expression Edward Boyden 108912...Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neurons Yulong Li 123308...Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neurons Yulong Li 123309...

  3. CRISPR Plasmids - Drosophila

    Type
    Collection
    .... When a repair template is present, the cell may repair a DSB using homology-directed repair (HDR) instead...be used with a repair template to introduce specific edits via homology-directed repair (HDR). ID Plasmid...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ...genomic changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the...nicks create a double-strand break (DSB) that is repaired using error-prone non-homologous end joining (...

  4. TALEN Guide

    Type
    Collection
    ...amino acid repeats. These repeats only differ from each other by two amino acids, their repeat-variable ... of 18 TAL effector repeats. The kit can be used to make arrays from 12-31 repeats. Perhaps one of the... to this DNA targeting ideal. Figure 1: (A) Representation of a zinc finger (ZF) nuclease. Zinc fingers...if not slightly lower. Figure 2: Simplified representation of the Voytas/Bogdanove Golden Gate TALEN kit... would expect that assembling relatively small, repeat-laden DNA regions into a single construct would...from Addgene , custom arrays consisting of 12-31 repeats can be assembled and inserted into a variety of...

  5. Genetic Code Expansion

    Type
    Collection
    ...tyrS gene knocked out, tyrTV gene replaced by tolC, and tyrU gene replaced by GentR). Expresses archaeal ...tyrTV gene replaced by tRNA variant that suppress the TAG nonsense codon, and tyrU gene replaced by tRNA ...tyrS gene knocked out, tyrTV gene replaced by tolC, and tyrU gene replaced by GentR) and release factor 1...biopolymers. Tips for Success Before beginning to reprogram the genetic code, there are several things to ... are true. Always express first with a control reporter gene – GFP for E. coli or mCherry-GFP for mammalian...RF1 function removed, with Ubiquitin-UAG-sfGFP reporter George Church 98565 C321.ΔClpS.Ub-UAG-sfGFP all...removed, ClpS inactivated, with Ubiquitin-UAG-sfGFP reporter George Church 174513 Syn61 No TCG, TCA, or TAG...

  6. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...- and serine-containing peptide linker (GGSGGS) repeat pET28CLY2 Peptide linker standard consisting of...of ECFP and EYFP connected by 2 GGSGGS repeats pET28CLY3 Peptide linker standard consisting of ECFP and...and EYFP connected by 3 GGSGGS repeats pET28CLY4 Peptide linker standard consisting of ECFP and EYFP connected... connected by 4 GGSGGS repeats pET28CLY5 Peptide linker standard consisting of ECFP and EYFP connected...connected by 5 GGSGGS repeats pET28CLY6 Peptide linker standard consisting of ECFP and EYFP connected by 6 GGSGGS...GGSGGS repeats pET28CLY7 Peptide linker standard consisting of ECFP and EYFP connected by 7 GGSGGS repeats...consisting of ECFP and EYFP connected by 8 GGSGGS repeats pET28CLY9 Peptide linker standard consisting of...

  7. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...Envelope and Packaging Plasmids Reporter Plasmids A few examples of reporter plasmids that can be used for... Lentiviral reporter vector expressing firefly luciferase. pCMV-FLuc - Retroviral reporter vector expressing...vesicular stomatitis virus G) are packaged as replication-defective virus particles with the SARS-CoV-2...pHAGE-CMV-Luc2-IRES-ZsGreen-W - Lentiviral dual reporter vector expressing firefly luciferase and ZsGreen...ZsGreen. NL4-3 mCherry Luciferase - Lentiviral dual reporter vector expressing mCherry and firefly luciferase...destabilized EGFP. Lenti-luciferase-P2A-Neo - Lentiviral reporter vector expressing firefly luciferase with neomycin...

  8. Promega Plasmid Collection

    Type
    Collection
    ... and more. Luciferase Reporters Luciferase (Link opens in a new window) reporter assays use light produced...Find luciferase reporter vectors for sensitive gene expression analysis and fusion vectors containing... workflows. The collection contains luciferase reporter vectors for sensitive gene expression analysis...collection. For example, search by response element, reporter, protein, or tag, such as "NFAT", "luciferase"... a new window) is a structural complementation reporter system composed of a Large BiT (LgBiT; 18 kDa)...Addgene Find additional information about luciferase reporter assays (Link opens in a new window) from Promega...blogs for technical tips and tools for genetic reporter assays (Link opens in a new window) and NanoBRET...

  9. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...modifications: replacement of the Cbh promoter with EF1alpha addition of an mCherry reporter variant nuclear...utilizes a CRISPR/Cas9 based approach to knockout, repress, and activate gene targets. To accomplish this ...advantage of the Zhang lab all-in-one (guide/Cas9/reporter) pX458 plasmid family. They have made several ...nuclear reporters via an H2B fusion shuttle plasmid for shuttling U6-guide cassettes to make dual guide ...fusion for gene and non-coding enhancer sequence repression dCas9-VPR fusion for gene activation CRISPR/Cas9...175573 and 175574 express dCas9-KRAB-MeCP2 for repression. Plasmids 175571 and 175572 express dCas9-VPR...

  10. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    .... When a repair template is present, the cell may repair a DSB using homology-directed repair (HDR) instead...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Fully...gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ...genomic changes, researchers use ssDNA or dsDNA repair templates with 1. homology to the DNA flanking ...

  11. CRISPR Plasmids - Zebrafish

    Type
    Collection
    .... When a repair template is present, the cell may repair a DSB using homology-directed repair (HDR) instead...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ...genomic changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the...

  12. CRISPR Plasmids - Parasites

    Type
    Collection
    .... When a repair template is present, the cell may repair a DSB using homology-directed repair (HDR) instead...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Eukaryotic...gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ...genomic changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the...

  13. COVID-19 Resources

    Type
    Collection
    ...activity reporter of SARS-CoV-2 main protease Mpro. (Unpublished) A FlipGFP-based activity reporter of SARS-CoV... also lists several luciferase and fluorescent reporter plasmids that have been used for measuring viral...method termed Specific High Sensitivity Enzymatic Reporter UnLOCKING (SHERLOCK and SHERLOCKv2). The Broad... termed DNA Endonuclease Targeted CRISPR Trans Reporter (DETECTR). Mammoth Biosciences has published information...(Link opens in a new window) CRISPR tools and reporters now available from Stanley Qi's lab. COVID-19 ... Coronavirus Resource Hub COVID-19 SARS-CoV-2 preprints from medRxiv and bioRxiv Elsevier's Novel Coronavirus...

  14. CRISPR Plasmids - C. elegans

    Type
    Collection
    .... When a repair template is present, the cell may repair a DSB using homology-directed repair (HDR) instead...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ...genomic changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the...

  15. Antibody Production

    Type
    Collection
    ...to generate high quality preparations. Following production, all antibody preps are titered and undergo...Addgene does not recommend repeated freeze thaw of antibodies and suggests preparing single-use aliquots before...Innovation Antibody Collection webpage. Antibody preparations developed at IPI may differ from Addgene antibodies...Sanger Sequencing Purified recombinant antibody preps contain low levels of co-purified plasmid from transfection...

  16. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR...Next-Generation Sequencing (NGS) to confirm library representation. CRISPR screening experiments require large...whether they are used to knockout, activate or repress target genes. Number of gRNAs - A given CRISPR ... taurus ) Library Size Genome-wide Subpool Viral Prep Available Yes No Clear Filters Name ID Library Type...Generation gRNAs per gene Total gRNAs Adamson DNA Repair CRISPRi Libraries 177663, 177664, 177670 Inhibition...A - The protospacers contained in the library represent all 4096 (4 6 ) combinations of 6 nucleotides....Editing Human Wei 3rd 3 53,502 sgRNA Pool for Streptococcus pneumoniae 170432 Knockout S. pneumoniae Veening...

  17. TALEN Expression Vectors

    Type
    Collection
    ...Mammalian expression vectors for TAL effector repeat arrays assembled using the REAL, REAL-Fast or FLASH... into which DNA fragments encoding TAL effector repeat arrays (assembled using the REAL, REAL-Fast or .... All three of these methods yield TAL effector repeat arrays with identical DNA and amino acid sequences...together with the Joung Lab Individual REAL TALE Repeat Plasmids in the Joung Lab REAL Assembly TALEN Kit...Biotechnol. 2011 ): ID Plasmid C-terminal 0.5 TALE repeat Binds 32285 JDS70 NI RVD A 32287 JDS71 HD RVD C...

  18. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ...has been used for dynamic tracking of repetitive and non-repetitive genomic loci, as well as chromosome ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Using...given region. For example, chromosome-specific repetitive loci can be efficiently visualized in living ... targeted sequences in close proximity. A non-repetitive genomic locus can also be labeled by co-delivering...

  19. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...plasmids, the cell may instead repair the break using homology-directed repair (HDR). HDR is much less error-prone...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ...the DNA, possibly resulting in frameshifts. If a repair template with high homology to the DNA surrounding...

  20. TALEN Engineering

    Type
    Collection
    ...developed three platforms for engineering TAL effector repeat arrays of any desired length: the REAL (Restriction... three of these platforms assemble TAL effector repeat arrays on a single architectural framework and ...methods are identical in sequence. TAL effector repeat arrays produced on this framework has yielded TALENs...Lab REAL Assembly TALEN Kit Individual REAL TALE Repeat Plasmids TALEN Expression Vectors for REAL, REAL-Fast...for Endogenous Human Genes TALENs for the EGFP Reporter Gene Additional Plasmids compatible with the REAL...
Showing: 41 - 60 of 123 results