We narrowed to 115 results for: REP

...modifications: replacement of the Cbh promoter with EF1alpha addition of an mCherry reporter variant nuclear...utilizes a CRISPR/Cas9 based approach to knockout, repress, and activate gene targets. To accomplish this ...advantage of the Zhang lab all-in-one (guide/Cas9/reporter) pX458 plasmid family. They have made several ...nuclear reporters via an H2B fusion shuttle plasmid for shuttling U6-guide cassettes to make dual guide ...fusion for gene and non-coding enhancer sequence repression dCas9-VPR fusion for gene activation CRISPR/Cas9...175573 and 175574 express dCas9-KRAB-MeCP2 for repression. Plasmids 175571 and 175572 express dCas9-VPR...

.... When a repair template is present, the cell may repair a DSB using homology-directed repair (HDR) instead...gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ...introduce specific genomic changes, researchers use DNA repair templates with homology to the DNA flanking the... our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs ...

...to generate high quality preparations. Following production, all antibody preps are titered and undergo...Addgene does not recommend repeated freeze thaw of antibodies and suggests preparing single-use aliquots before...Innovation Antibody Collection webpage. Antibody preparations developed at IPI may differ from Addgene antibodies...Sanger Sequencing Purified recombinant antibody preps contain low levels of co-purified plasmid from transfection...

...drives expression of RFP as a reporter. See plasmid 17618 for GFP reporter. Linzhao Cheng 17452 pLenti ...Expresses shRNA under mouse U6 promoter. CMV-EGFP reporter cassette is included to monitor expression. Luk...Expresses shRNA under mouse U6 promoter. CMV-EGFP reporter cassette is included to monitor expression. Stephan...86849 pBOB-EF1-FastFUCCI-Puro 3rd Encodes FUCCI reporters for cell cycle analysis. Puromycin selection. ...Addgene blog: Tips for Titering Your Lentiviral Preps Content last reviewed: 23 October 2025 Do you have...

...be used with a repair template to introduce specific edits via homology-directed repair (HDR). Figure ...nicks create a double-strand break (DSB) that is repaired using error-prone non-homologous end joining (...Figure 1: Overview of Cas9 nickase cleavage and repair. Browse, sort, or search the tables below for CRISPR... our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs ...

...has been used for dynamic tracking of repetitive and non-repetitive genomic loci, as well as chromosome ...given region. For example, chromosome-specific repetitive loci can be efficiently visualized in living ... targeted sequences in close proximity. A non-repetitive genomic locus can also be labeled by co-delivering... our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs ...

...developed three platforms for engineering TAL effector repeat arrays of any desired length: the REAL (Restriction... three of these platforms assemble TAL effector repeat arrays on a single architectural framework and ...methods are identical in sequence. TAL effector repeat arrays produced on this framework has yielded TALENs...Lab REAL Assembly TALEN Kit Individual REAL TALE Repeat Plasmids TALEN Expression Vectors for REAL, REAL-Fast...for Endogenous Human Genes TALENs for the EGFP Reporter Gene Additional Plasmids compatible with the REAL...

...limited use label licenses (LULLs). Titer For AAV preps produced through our Packaged on Request service... we may expect lower titers for a particular AAV prep, mostly likely due to one of the following reasons...moving forward with a helpful solution. You must reply to that email within 10 business days in order to...been completed at the time of shipment. If any discrepancies are identified during this analysis, you will...moving forward with a helpful solution. You must reply to this email within 10 business days in order to...

...which is not required for the transcription or replication of the genome within infected cells, but is required... cells that lack the G protein, the virus can replicate but the newly formed viruses are unable to infect...starter cell via the EnvA receptor. RABV is able to replicate in the starter cell using the G-protein, which...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...

...editing, empty backbones, fluorescent proteins, reporters, biosensors, and Cre expression plasmids. Large...studying diseases, are listed below: Lipid droplet reporters - Richard White Lab. An imaging platform to study...using red light. LipoGlo - Steven Farber Lab. A reporter system that allows direct assessment of lipoprotein...International Resource Center (ZIRC) - A US-based repository for zebrafish strains and materials and information...European Zebrafish Resource Center (EZRC) - A repository at the Karlsruhe Institute of Technology (KIT...

...leucine-rich repeat-containing G protein-coupled receptor 4 GPR48 LGR5 leucine-rich repeat-containing G... to the formation of a high avidity BCR through repeat cycles of expansion. Chemokines Chemokines, or ... to the formation of a high avidity BCR through repeat cycles of expansion. Symbol Name Synonyms AKT1 ...MGC102867 SEMA5A sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain ... semF SEMA5B sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain ...LGR1, MGC141667, MGC141668, ODG1 GAL galanin prepropeptide GALN, GLNN, GMAP, MGC40167 GALP galanin-like...GHRFR, GHRHRpsv, GRFR GHRL ghrelin/obestatin prepropeptide MTLRP, obestatin GHSR growth hormone secretagogue...

...demethylation DNMT3A — repression through cytosine methylation MQ1 — repression through cytosine methylation...methylation with improved kinetics LSD1 — repression through targeted removal of H3K4 and H3K9 methylation... our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs ...

... modification, including shuttle, integration, reporter, and tagging vectors. Metabolism Browse plasmids...regulatory elements, including promoters, terminators, repressors, activators, and more. Also contains pre-assembled...BioBrick Box Mukhopadhyay Lab Efflux Pumps Voigt Lab Repressors , Terminators , Light Signaling , Sigma Factors...Biological Parts (Link opens in a new window) — Repository of community-created BioBricks JBEI (Link opens...

...Microbiology Plasmids and Resources Addgene’s repository contains plasmid tools for a range of microbial...distributes cannot be used to reconstitute self-replicating microbes or recreate the diseases they cause....Salmonella sp. Shigella sp. Staphylococcus sp. Streptococcus sp. Synechococcus sp. Thermus sp. Vibrio sp....plasmid tools for the community that aren't in the repository? Help us widen our resources for the community...

...Find prime editor plasmids for (search and replace) genome editing....pegRNA Resources Prime editing is a “search and replace” gene editing method in which a reverse transcriptase...heteroduplex is resolved by the cell’s mismatch repair (MMR) system. Alternatively, if the edited 3’ flap... our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs ...

...glutamatergic neurons, Cre payload. Must use with AAV8-DIO-Reporter viruses infected in postsynaptic cells. 5, 8 Don...express Cre, Flp payload. Must use with AAV8-fDIO-Reporter viruses infected in postsynaptic cells. 5, 8 Don...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...

... engineered small proteins derived from ankyrin repeat proteins. These are antibody mimics instead of ...isotype. Overview of application Fix your samples. Prepare your samples using permeabilization, antigen retrieval...and controls follow that of IF (above). Sample preparation, signaling excitation, and assay readout vary...must also be run. Unique samples (non-biological replicates) often each require their own set of controls...conjugating them in the lab? How will the sample be prepped, and will this or other factors affect epitope ...

...bound to DNA, and the break can be repaired by the cell’s endogenous repair mechanisms. Cas3 belongs to the... our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs ...

...and innovation programme and EFPIA. Figure 1: Representation of RESOLUTE mission. The combination of metabolomics...deorphanizing engine. Genetic and proteomic interactions represent the framework that provides biological context...the Resolute Partnership using their RESOLUTE repository feedback form (Link opens in a new window) . ...

...Serotype Testing AAV These AAV encode fluorescent reporters and can be used to compare the tropism of different...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...