...precise gene editing. These edits can include mutations seen in human patients, protein tags, or loxP/...off-target activity due to rationally designed mutations. Researchers have expanded the CRISPR field beyond... DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP) using CRISPR. Biochem Biophys Res...

... delete genes from or introduce new genes and mutations to the bacterial genome itself. Manipulating the...to study the effects of protein expression in stationary phase. In these cases you may want to control...polymerase subunit ω. Transcription factor binding to a putative promoter region cloned into pH3U3-zif268 (omega...

...investigators who will use state-of-the-art experimental and computational approaches to model, predict...

...SUMO, GST, MBP, CBP, S-tag, TAP tag, TRX, or V5) Mutations Wild type (wt) Codon-optimized expression (recode...making pseudovirus (Ctrunc) ID Plasmid Description Mutations Tags Industry Additional Resources Read Addgene's...

...approximately 450 identified members. Many SLCs have mutations associated with disease and could become targets...and innovation programme and EFPIA. Figure 1: Representation of RESOLUTE mission. The combination of metabolomics...

...or to knockdown genes with potentially harmful mutations in therapeutic settings. As a tool in mammalian...RISC includes argonaute protein 2 (Ago2), which facilitates cleavage of the target. RNAi using siRNA follows...

... create A->G (or T->C on the opposite strand) mutations. ID Plasmid Gene/Insert Promoter Selectable Marker...enCHIP system uses an anti-FLAG antibody to immunoprecipitate FLAG-tagged Cas9. Design your gRNA sequence...

...ZFNs) are effective tools for making targeted mutations in zebrafish. With support from the NIH and guidance...hydroxylase (dbh) OZ525 and OZ526 OPEN OPEN tCCCATCCTAtcgttGCATGAGCTc mdka (midkine-related growth factor) OZ527...

...fragments can be used in experiments such as immunoprecipitation and super-resolution microscopy. Two commonly...amber codons in scFvs A new expression vector facilitating production and functional analysis of scFv antibody...

...integrates these signals to determine the nutritional state of the cell and regulates the switch from anabolic...multiple familial cancers are caused by inactivating mutations in negative regulators of mTORC1. The hyperactivation...

...i.e., genome containing) AAV particles within representative vector preparations was quantified with electron...for viral production are propagated in the endA -mutated NEB Stable strain of E. coli . In addition, plasmids...

...Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden Citation Information When using the Caltech serotypes in...L4 - AAV.CAP-B10 with GRE or transgenic animal Excitatory - AAV.PHP.N with GRE or transgenic animal For...

...Rat Mouse IgG2a 140065 Anti-SCG10/Stathmin-2 [L5/1R] SCG10/Stathmin-2 Rat Mouse IgG2a 140066 Anti-Co-Rest...83R] BAF53a Human Mouse IgG2a 206558 Anti-ATAT1 [N347/42R] ATAT1 Human Mouse IgG2a 206559 Anti-Foxi2 [N349...distinct IgG subclass from the conventional mAb to facilitate multiplex labeling with subclass-specific secondary...

...Next-Generation Sequencing (NGS) to confirm library representation. CRISPR screening experiments require large...Human Wei 3rd Varies 12,472 pairs HR700_TP53 Exon Mutation Libraries 229137–229140 Donor Vector Human Stiewe...

...Cre-dependent AAV expression of humanized ChR2 (with H134R mutation) fused to eYFP for optogenetic activation Viviana...Service . Addgene provides a viral service to help facilitate scientific research. Addgene's AAV are made from...

...Plasmids Plasmid Gene/Insert Vector Type Backbone Mutations Tags Industry PI...

...Oxford Plasmid Gene/Insert Vector Type Backbone Mutations Tags PI...

...Plasmids - Stockholm Plasmid Gene/Insert Vector Type Mutations Tags Industry PI...

...Toronto Plasmid Gene/Insert Vector Type Backbone Mutations Tags Industry PI...

...Plasmids ID Plasmid Gene/Insert Vector Type Backbone Mutations Tags PI...