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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 57 results for: des.2

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Showing: 41 - 57 of 57 results

  1. E11 Bio PRISM Collection

    Type
    Collection
    ...optical imaging down to individual synapses. Figure 2: PRISM workflow. A) Stochastic infection of pooled...light microscopy. In brain tissue, these protein barcodes allow for efficient machine-learning segmentation...enhancers for morphological readout. How to Use PRISM Barcodes are delivered as pooled AAVs either directly to...bias in packaging representation. To read out the barcodes, each protein tag can be imaged with a corresponding...combinations of protein bits. B) Multiplex imaging of barcodes with expansion microscopy and reconstruction of...Sheridan et al. (2025). PRISM Plasmids ID Plasmid Description Tags Additional Resources PRISM was developed...., & Payne, A.C. (2025). Combinatorial protein barcodes enable self-correcting neuron tracing with nanoscale...

  2. Institute for Protein Innovation

    Type
    Collection
    ... proteins are currently under development. Figure 2: Key adhesion proteins and receptor–ligand pairs bridge...IGDCC3 (PUNC) Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended Applications PI...signaling during development. The IPI collection includes anti-glypicans that accurately detect glypican...scientists worldwide. ID Recombinant Antibody Description Isotype Reactivity Recommended Applications PI...neurological disorders. The initial collection includes antibodies to Neurexin and Neuroligin (Link opens...Neuroligin Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended Applications PI... Integrin Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended Applications Return...

  3. AAV for Neuronal Tracing

    Type
    Collection
    ... and G-protein is delivered to the starter cell. (2) The starter cell now expresses TVA and G-protein....Vectors for Monosynaptic Neuronal Tracing ID Name Description Serotype PI 52473 pAAV-synP-FLEX-splitTVA-EGFP-B19G...

  4. Distribution to Industry

    Type
    Collection
    ...and HaloTag Fusions from Promega COVID-19 SARS-COV-2, ACE2, and CRISPR tools Fluorescent Proteins from ...Plasmid Gene/Insert PI Kits Kit name Type PI Description High Complexity Golden Gate Assembly Standards...PDB-deposited), disulfide-free, mesophilic, and designed to be diverse in topology, size, and isoelectric...Pooled Libraries Pooled Library name Type PI Description HR700_TP53 Exon Mutation Libraries CRISPR Thorsten...Parkinson's Disease; For target validation and drug design Antibodies Addgene distributes ready-to-use recombinant...materials and intellectual property rights, and provides liability protection for material providers. Addgene's...

  5. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...transient mammalian expression 101126 pACP-tag (m)- 2 Vector ACP/MCP-tag Empty backbone for stable and transient...label of their choice according to the methods described by George et al. (2004). The ACP and SFP Synthases...respectively) may be expressed and purified as described previously in Stachelhaus et al. (1998) References...

  6. Deisseroth INTRSECT Collection

    Type
    Collection
    ...dual-transgenic recombinase-expressing mouse lines 2–9 and combinations of transgenic recombinase-expressing...delivering additional recombinases 10–14 . Figure 2: INTRSECT works by inserting short, intronic sequences... experimental design. How-to guide (Link opens in a new window) for the molecular design and testing of...interesting in implementing intersectional experimental design. The Deisseroth Lab maintains a Standard Operating...Gray Facilitate the Itch-Scratching Cycle via Descending Regulation. Neuron 101(1):45-59. PubMed (Link...

  7. Antibody Production

    Type
    Collection
    ...for shipping stability by incubating at 37 °C for 2 weeks, freezing at -80 °C, thawing on ice, and then...protocols, titering methods, and quality control are described below. Information for a specific antibody can...

  8. Brain Initiative Collection

    Type
    Collection
    ...Gordon Fishell 83899-AAV1 pAAV-mDlx-GCaMP6f-Fishell-2 GCaMP6f expression in forebrain GABA-ergic interneurons...Gordon Fishell 83899-AAV9 pAAV-mDlx-GCaMP6f-Fishell-2 GCaMP6f expression in forebrain GABA-ergic interneurons...Gordon Fishell 83899-AAVrg pAAV-mDlx-GCaMP6f-Fishell-2 GCaMP6f expression in forebrain GABA-ergic interneurons...brain cells and circuits during behavior. Long desired by researchers, the revolutionary dynamic picture... with Addgene materials. Plasmids ID Plasmid Description PI Viral Preps Addgene distributes ready-to-use...Learn more about our viral service ID Viral Prep Description PI 65417-AAV8 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine...and proximal dendrites in a viral vector Hillel Adesnik 107708-AAV9 pAAV-hSynapsin- soCoChR-GFP A somatic...

  9. Tetracycline Inducible Expression

    Type
    Collection
    ...hSyn promoter rtTA3 Wei Xu 26803 pEnt L1L3 EF1a-tTA-2 Gateway entry vector to express tTA from EF1α promoter...expression and improved dynamic range . Hum Gene Ther, 22 (2), 166–176. https://doi.org/10.1089/hum.2010.099 (Link...promoters (sometimes generally called pTet) have been designed by combining tet O sequences with elements like...plasmids use "TetO" or "Tet-O" in their name or description, meaning they contain a TRE or tet O and can ...as much flexibility and control as a dual vector design, but it is easier to implement and ensures that...transduction (transient or stable) may depend on the desired sensitivity, level of induction, or amount of background...expression of your gene of interest. ID Plasmid Description Transactivator Promoter PI 21915 Tet-pLKO-puro...

  10. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...expressing Rep2 and Cap1 James M. Wilson 232199 pAAV2/2 AAV2 AAV packaging plasmid, expressing Rep2 and Cap2...required for AAV viral replication. This collection provides a curated selection of transfer, packaging, and... packaging eligibility, and more. ID Plasmid Description PI Return to top AAV Packaging Plasmids Packaging...for all available serotypes. ID Plasmid Capsid Description PI 112862 pAAV2/1 AAV1 AAV packaging plasmid,...repository for AAV vector production. ID Plasmid Description PI Return to top Other Resources Addgene's Viral...

  11. TALEN Plasmids and Kits

    Type
    Collection
    ...vectors designed for use with the Voytas Golden Gate TALEN Kit and are used in place of pTAL1, 2, 3, or...Sangamo)-Destination Pawel Pelczar pCAG-T7-TALEN(Sangamo)-Destination constructs were designed for optimal...pC-GoldyTALEN and RCIsricpt-GoldyTALEN are designed as destination vectors for the Voytas lab Golden Gate ...Plasmids pTAL5-BB and pTAL6-BB are designed as alternative destination vectors for the Golden Gate TALEN...TALEN(Sangamo)-FokI-KKR-Destination 40132 pCAG-T7-TALEN(Sangamo)-FokI-ELD-Destination Golden Gate TALEN 2.0...Mendenhall The TALE plasmid vectors described on this page are designed to target and edit the epigenome ...with custom repeat arrays. TALEN Kits TALEN Kit Description Depositing PI FusX TALEN Assembly System Contains...

  13. Fluorescent Proteins: FRET

    Type
    Collection
    ...referred to as intermolecular or bimolecular FRET) or (2) conformational changes within a protein where the...maturation time, and other environmental factors. When designing your experiment, be sure to refer to published...proteins fused with linkers of varying lengths. Description Article ECFP-EYFP FRET standards, fusion proteins...Biosensor Database (Link opens in a new window) designed by Eric Greenwald and the Jin Zhang Lab at UCSD...

  14. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...sequence YFP Thorben Dammeyer 54520 mCherry-Peroxisomes-2 Peroxisomes Peroximal targeting signal (SKL) mCherry...Centrin2 Centrioles (dependent on cell cycle) Centrin-2 EGFP Erich Nigg 41151 pEGFP Cep170 C-term Centrioles... 36991 Desmoglein2-mCherry Desmosomes Desmoglein2 mCherry Kathleen Green 36989 1105-Desmoglein2-FLAG-eGFP... PIP2a mCherry Maria Harrison 61206 pCMU-PDESr Plasmodesmata (Plants) PDLP1 mCherry Maria Harrison 61200...-eGFP Desmosomes Desmoglein2 EGFP Kathleen Green 74260 pKanCMV-CX4.3-7aa-mClover3 Gap junctions Connexin...Davidson 40907 pDEST/hCx43-EGFP-N1 Gap junctions Connexin 43 EGFP Robin Shaw 49861 pDEST-mCherry-GJA1-20k-N1...Actin filaments beta-Actin EGFP Robert Singer 40908 pDEST/LIfeAct-mCherry-N1 Actin filaments LifeAct mCherry...

  15. Neurodegeneration Research Collection

    Type
    Collection
    ...different inherited genes: Presenilin 1, Presenilin 2, and APP. The majority (> 90%) of individuals develop...brain. The cause of this neuron death is unknown. Despite the idiopathic (having no specific known cause)...scientific community at low cost, with rapid delivery. Includes antibodies, viral vectors, animal models, purified...

  16. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...- Bacterial Expression mScarlet-I3 568 592 68 4.2 2 min Monomer pmScarlet-I3_C1 - Mammalian Expression... Vector - Mammalian Expression pENTR4-CLIPf (w877-2) - Gateway Entry Vector pET51b-His-TEV-CLIP-tag - ...fluorescence to reach half its maximal value. Structure describes the oligomeric state of the protein. Many FPs ...Chloralkane (CA) pMH-Halo tag - Mammalian Gateway Destination Vector pENTR4-HaloTag (w876-1) - Gateway Entry...

  17. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...tetraloop and stemloop 2 and EF1a-zeo resistance marker pLenti CMV/TO GFP-Zeo DEST (719-1) - 3rd gen lentiviral...CMV GFP DEST - Lentiviral Gateway destination vector for gene expression pLenti CMV/TO Zeo DEST - Tet-inducible...lentiviral vector for ORF expression pDest-565 - Gateway destination vector for bacterial expression; His...Membrane localization pWZL-Neo-Myr-Flag-DEST - pDest vector that adds an N-terminal myristoylation...lentiviral Gateway destination vector, expression, CMV/TO promoter, GFP-Zeo pLenti CMV/TO Zeo DEST - ...expression pLenti CMV GFP DEST - Lentiviral Gateway destination vector for gene ...needed for replication in the host cell and are designed for a user to insert a gene of interest. They ...
Showing: 41 - 57 of 57 results