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Showing: 41 - 60 of 131 results

  1. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...subject to Addgene’s Terms of Purchase and the applicable material transfer agreements (MTAs) and limited...without a guaranteed titer. Any titer expectations communicated by email will supersede the general guarantees...step of the QC process, viral genome sequence verification, may not yet have been completed at the time...longer turnaround time. Any timing expectations communicated by email will supersede the general estimated...be required by your country for the import of biological research materials. Please be aware that additional...contact you directly regarding these charges, if applicable....

  2. AAV Molecular Tools

    Type
    Collection
    ... Tools for Affinity Purification These AAV encode tools for affinity purification (which can be used for...tet-off transactivators and tools for affinity purification (TRAP). Viral... Tools Tetracycline Transactivators Affinity Purification Neurophysiology Cell Ablation Genome Engineering...of the voltage-gated Na+ channel mNaChBac and (physically separate) tdTomato. 8 Massimo Scanziani 34910...constitutive Expresses mouse HAPLN1 mutant lacking the lectican binding domain and fused with cysteine-free GFP...

  3. Zebrafish Plasmid Collection

    Type
    Collection
    ...horizontal stripes on the sides of the adult body, is a tropical freshwater fish in the minnow family. It has long...inflammation and infection, regeneration, human neurological disorders, microbiota studies, and drug discovery...Cell lineage tracing in multicellular systems typically relies on genetic markers, somatic mutations, ...types. CreLite - George Eisenhoffer Lab. An optogenetically controlled Cre/loxP system that enables precise...the international research community. The Latin American Zebrafish Network (LAZEN) - LAZEN aims to increase...advanced training of young scientists in Latin America. The Zebrafish Book - A guide for the laboratory...

  4. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...Brain Science AAV Enhancer Collection Plasmids Publications Resources Enhancer AAV plasmids are plasmids...following regions: Isocortex Hippocampal formation Cortical subplate Striatum Pallidum Hypothalamus Cerebellum...pAAV-AiE2051m-minBG-SYFP2-WPRE3-BGHpA AiP1277 AiE2051m SYFP2 Claustrum Cortical subplate 220711 pAAV-AiE2051m-minBG-iCre(R297T...R297T)-BGHpA AiP1786 AiE2051m Cre Claustrum Cortical subplate 230518 pAAV-AiE2051m-minBG-FlpO-WPRE-HGHpA ...pAAV-AiE2051m-minBG-FlpO-WPRE-HGHpA AiP1211 AiE2051m FlpO Claustrum Cortical subplate 191720 pAAV-AiE0743m_3xC2-minBG-SYFP2-WPRE3-BGHpA ...= Substantial non-specific labeling observed Publications Ben-Simon, Y., Hooper, M., Narayan, S., Daigle...and transgenic mouse lines for genetic access to cortical cell types. Cell , 188 (11), 3045–3064. https:...

  5. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...Insert Promoter PI Publication Drosophila ID Plasmid Gene/Insert Promoter PI Publication Yeast ID Plasmid...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...Protocols gRNA Design Tools CRISPR Blog Posts A catalytically inactive Cas9 (dCas9) is fused to FokI nuclease...Plasmid Gene/Insert Promoter PI Publication Do you have suggestions for other plasmids that should be added...

  6. Synthetic Biology - Cloning and Genomic Tools

    Type
    Collection
    ...Genomic Tools for library construction, genomic modification, and cloning systems. Jump to Table: Cloning... or sort by the table headings. Click on the publication link to view all plasmids available from the ...Plasmid Description Gene/Insert Vector Type PI Publication Back to Top Shuttle Vectors Search the table ... or sort by the table headings. Click on the publication link to view all other plasmids from that article...Description Vector Type Selectable Marker PI Publication Back to Top Do you have suggestions for other...

  7. Antibodies

    Type
    Collection
    ...expressed from plasmids. These antibodies undergo application-specific validation and consistent quality control...and provide experimental data for recommended applications. Addgene values transparency and accessibility...you choose and use antibodies, with an expert technical support team ready to answer your questions. All...antibodies developed and validated for many research applications. Browse Antibodies Production Interested in ... storage techniques, and explain common applications. View Guide Need Some Help? Browse some of our...

  8. CRISPR Plasmids - Repress Gene Expression

    Type
    Collection
    ...Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter PI Publication Return to ...Interfere Mammalian Bacteria Plant Yeast Resources Catalytically dead Cas9 (dCas9), or dCas9 fused to a transcriptional...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene/Insert Promoter...Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has a large selection...

  9. CRISPR References and Information

    Type
    Collection
    ...pooled library amplification 1 vector system: lentiCRISPR v2 2 vector system: lentiCas9-Blast and lentiGuide-Puro...Labs. ACEofBASEs (Link opens in a new window) Identification of sgRNA target sites and off-target evaluation... Target Finder (Link opens in a new window) Identification of gRNA target sequences from an input sequence...window) A collection of protocols, materials, and publications by members of the Division of Signaling and ...Download protocol Addgene CRISPR pooled library amplification CRISPR pooled libraries Protocol at Addgene ...

  10. CRISPR Plasmids - Single-Strand Break (Nick)

    Type
    Collection
    ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert...Insert Promoter Selectable Marker PI Publication Return to top Drosophila ID Plasmid Gene/Insert Promoter ...Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable Marker...Marker PI Publication Return to top Yeast ID Plasmid Description Gene/Insert Promoter Selectable Marker ...Marker PI Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...

  11. Fluorescent Protein Guide

    Type
    Collection
    ...small biomolecules or other physiological intracellular processes with genetically encoded fluorescent biosensors...Use this guide to learn about the applications of fluorescent proteins and to find the plasmids that ... Use this guide to learn more about the many applications of fluorescent proteins and to find plasmids...Microscopy Resource Center (Link opens in a new window) Leica Microsystems Resources for Microscopy in Life Sciences...

  12. Optogenetics Plasmid Collection

    Type
    Collection
    ...optogenetics plasmids such as microbial opsins and optical switch tools to activate, inhibit, and manipulate...Optogenetics Plasmid Collection Microbial Opsin Plasmids Optical Switch Plasmids Additional Resources Optogenetics...proteins. These fusions can then be used as an optical switch to control protein interactions, dimerization...response spectra) of different types of opsins and optical switches available in these tables, refer to Addgene's... Eligible for Viral Packaging PI Return to top Optical Switch Plasmids Browse, sort, or search the table...

  13. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...unraveling DNA using its helicase properties, cleaving the targeted strand non-specifically along the way. Cas3...Promoter PI Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter PI Publication Return to ...top Plant ID Plasmid Gene/Insert Promoter PI Publication Return to top CRISPR Resources Addgene has a ...

  14. Synthetic Biology - Overview

    Type
    Collection
    ...International Genetically Engineered Machine Foundation Registry of Standard Biological Parts - Repository...biology describes the rational engineering of biological systems for novel functions. Addgene is working...Browse plasmids related to cloning and genomic modification, including shuttle, integration, reporter, and...Fungal Algal Featured SynBio Deposits Alper Lab Y. Lipolytica Promoters Anderson Lab Plasmids and Phagemids...

  15. Luciferase Plasmid Collection

    Type
    Collection
    ...advantages and disadvantages depending on the application, and different luciferases require different ... course experiments, but is too dim for some applications. NanoLuc® is small (19 kDa), very bright, and... available, making it ideal for a variety of applications. To learn more and plan your experiment, read...Malcolm Moore 51819 Syn-ATP Firefly (modified) CMV Optical reporter of presynaptic ATP Timothy Ryan 24348 ...Engineered to use the substrate Akalumine with significantly brighter signal and emission in the near-infrared...OgNLuc ) fusions for BRET-based imaging and other applications. And see PalmGRET , a membrane-anchored version...

  16. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...] VGlut1 Rat Mouse IgG2a 128639 Anti-Versican [N351/23R] Versican Mouse Mouse IgG2a 128640 Anti-Pan-Ankyrin... Lgi1 Mouse Mouse IgG2a 177528 Anti-Brevican [N294A/10R] Brevican Rat Mouse IgG2a 177529 Anti-GluN1 [N308...ARHGAP4 Mouse Mouse IgG2a 190309 Anti-Brevican [N294A/6] Brevican Rat Mouse IgG2a 190311 Anti-NSD1 [N312...Ppp1r9a Mouse Mouse IgG2a 211585 Anti-Versican [N351/24R] Versican Rat Mouse IgG2a 211587 Anti-GluN3A/NR3A...Synaptotagmin-7 Mouse Mouse IgG2a 220429 Brevican [N294A/10R-2b] Brevican Rat Mouse IgG2a 220430 BAF53b [N332B...external) Human Mouse 206772 Brevican scFv [N294A/6] N294A/6 scFv Brevican Rat Mouse 206773 GluN3A/NR3A...extensively validated for neuroscience research applications. A substantial number of these have been cloned...

  17. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ... a prime editing guide RNA (pegRNA). As with a typical gRNA, the pegRNA is designed with a spacer that...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert...Insert Promoter Selectable Marker PI Publication Return to top Drosophila ID Plasmid Gene/Insert Promoter ...Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable Marker...Marker PI Publication Return to top Empty Prime Editing gRNA Vectors A selection of empty gRNA vectors suitable...

  18. Immunology Research Plasmids and Resources

    Type
    Collection
    ...K-RAS, N-RAS, RASH1 ICAM1 intercellular adhesion molecule 1 BB2, CD54, P3.58 ICAM2 intercellular adhesion...repurposing of open access immunological assay data for translational and clinical research. Sci Data. Feb...and adaptive immune signaling and cell-cell communication. Plasmid...specific to any particular pathogen. It includes physical barriers such as the skin and mucosa, antimicrobial...molecules that enable cells of the immune system to communicate with each other across long distances. During...with virus and, like other cytokines, enable communication between cells of the immune system. Interleukins...are secreted by immune cells and coordinate communication between immune cells over the course of an immune...

  19. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...into a genomic attP site. So far, their practical application has been limited by low integration efficiency...other recombinase systems. Enzyme Target Site Canonical Sequence Structure (Length) Common Variants Cre...repeats + 4 bp spacer (32 bp) Currently, only the canonical rox site is widely used. Flp FRT GAAGTTCCTATTCCGAAGTTCCTATTCtctagaaaGTATAGGAACTTC...excise the shRNA to return gene expression to physiological levels. In lox-STOP-lox shRNA constructs, Cre...

  20. CRISPR Plasmids - Drosophila

    Type
    Collection
    ... Promoter PI Publication Return to top Cut ID Plasmid Gene/Insert Promoter PI Publication Return to top...Promoter PI Publication Return to top Prime Edit ID Plasmid Gene/Insert Promoter PI Publication Return to...
Showing: 41 - 60 of 131 results