...expressing mCherry and firefly luciferase. pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro - Lentiviral...Lentiviral vector expressing destabilized EGFP. Lenti-luciferase-P2A-Neo - Lentiviral reporter vector expressing...expressing firefly luciferase with neomycin resistance. Other Resources Protocols Bloom lab - Protocol and...

... director for more information. Why MDH? MDH is stable, inexpensive to assay, and easy to purify using...institutions. With these plasmids, faculty can begin a sustainable MDH-related CURE focusing on protein expression...Link opens in a new window) — Learn how to get started with a CURE, explore MDH projects, find teaching...

...: Basic AVEXIS : A list of all you need to get started with AVEXIS, including positive and negative controls...receptors Human erythrocyte Babesia microti blood stage proteins P. falciparum sporozoite cell surface and...invasion by Plasmodium falciparum. Crosnier C, Bustamante LY, Bartholdson SJ, Bei AK, Theron M, Uchikawa...

...Plasmids rsTagRFP 440 / 567 585 / 585 0.02 / 4.0 6.6 43 min Prone to dimerization pBAD/HisD-rsTagRFP - Bacterial...Structure describes the monomeric or oligomeric state of the protein. Many FPs are monomers at low concentration...pLV-eGFP - Mammalian Lentiviral Expression Ac5-STABLE1-neo - Insect Expression pCS2+8CeGFP - Zebrafish...

...Ras Pathway p53 Pathway Tackling Cancers’ Drug Resistance with a New Screening Kit Background mTOR Pathway...integrates these signals to determine the nutritional state of the cell and regulates the switch from anabolic... Kit (Sabatini & Wood) Tackling Cancers’ Drug Resistance with a New Screening Kit (from David Sabatini...

...Co-Founder and CSO, Addgene Organizing Committee: Dr. Constance Cepko, Harvard Medical School Dr. Melina Fan, ..., Dr. Meghan Rego, Addgene Confirmed Speakers Constance Cepko Harvard Medical School Ali Cetin Allen Institute...technology or its application? What are some current obstacles that researchers face right now (e.g., not enough...

...dcas9 gene on a p15A vector with chloramphenicol resistance. The second plasmid ( pgRNA_bacteria ) contains...expression cassette on a ColE1 vector with ampicillin resistance, wherein the N20 can be custom designed to target...gene 46919 pMLS-SV40-EGFP Target EGFP gene that is stably integrated into HEK293 genome 46922 pSNR52-sgTEF1...

...72258 Expresses FLPe, copGFP and the puromycin resistance from the CB promoter pCDH-CB-FLPe-P2A-tdTomato...copGFP-T2A-Puro 72263 Express copGFP and the puromycin resistance gene from the EF1 promoter pCDH-CMV-mCherry-T2A-Puro...-T2A-Puro 72264 Express mCherry and puromycin resistance gene from the CMV promoter pCDH-CMV 72265 Express...

...to a reverse transcriptase (RT) is capable of installing targeted insertions, deletions, and all possible... Stupar 62200 pBUE411 OsU3 yes, cut S. pyogenes Basta Chen 62203 pHUE411 OsU3 yes, cut S. pyogenes Hyg...Hygro Kim 78097 pBAtC U6 AarI yes, cut S. pyogenes Basta Kim 71286 pHEE401 U6-26p BsaI yes, cut S. pyogenes...

...polymerase 165501 pOpen-GBDpol Pyrococcus sp. Heat-Stable DNA Polymerase 165502 pOpen-9N7polA (CT) 9 degrees...P45 165572 pOpen-GBDpol (exo) Pyrococcus sp. Heat-Stable (exo-) DNA Polymerase 165573 pOpen-phi29pol phi29...DNA-binding protein 165549 pOpen-ETSSB Extreme Thermostable Single-Stranded DNA-Binding Protein 165579 pOpen-dromedNK...

...Tribolium castaneum BsaI none S. pyogenes Averof p(U6b-BsaI-gRNA) 65956 Other/Tribolium castaneum BsaI none...Hygro Yang pBUE411 62200 Plant yes, cut S. pyogenes Basta Chen pKSE401 62202 Plant yes, cut S. pyogenes Kan...Kim pBAtC 78097 Plant AarI yes, cut S. pyogenes Basta Kim pMZ283 52224 Yeast CspCI none S. pyogenes URA4...

...sequences corresponding to every previously observed instance of human serine phosphorylation ( Rinehart Human...in need of reference spectra or phosphopeptide standards for quantitative assessments. The mode #1 phosphosite...lactamase S68TAG Plasmid 69118 E17TAG GFP zeo resistance Strain 34929 BL21ΔserB Strain 52055 EcAR7 Plasmid...

...gene of interest rtTA or TetR expression plasmid Stable cell lines can be made that continuously express...lines Tet-On Advanced; Plasmid 26730 has hygro resistance 3rd generation rtTA On Campeau 25434 pMA2640 ...underlying expression of Tn10 encoded tetracycline resistance. Hillen W and Berens C. Annu Rev Microbiol . ...

...barrier integrity, and using CRISPR screens to understand more globally how neurons function. They are ...and death of dopamine-producing neurons in the substantia nigra, one of the movement control centers in...Study the C terminal domain of TDP-43 to better understand its function and pathology. Mohanty et al. Proc...

...Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic...lab. Plasmid Description Gene/Insert Antibiotic Resistance PI Publication Back to Top Do you have suggestions...

...Consortium (Link opens in a new window) aims to understand how genomic variation affects genome function...brings together teams of investigators who will use state-of-the-art experimental and computational approaches...

...efforts within the KLF community have lead to the establishment of this shared resource on Addgene. If you have...with Addgene or email [email protected] to get started. Genes KLF gene pages with information on available...

...factors for cellular programming or cancer drug resistance....applications such as cellular programming or cancer drug resistance. Protocols & References A transcription factor...

...for transitioning between anabolic and catabolic states. Ras Ras is a GTPase that is essential for regulating...Library (Frank Stegmeier) Tackling Cancers’ Drug Resistance with a New Screening Kit (David Sabatini & Kris...

...RNA sequence, they adopt an enzymatically active state and can bind and cleave additional RNAs regardless...homology to the crRNA. This activity provides a stark contrast to Cas9 and Cpf1, which require that each...