We narrowed to 112 results for: tra

...editing to easily engineer specific transgenic worm lines. The transparent body of the C. elegans hermaphrodite...Caenorhabditis elegans. Mos1 Based Transgenesis miniMos Transgenesis Kit - Erik Jorgensen Lab. This kit...consists of 959 somatic cells, which makes them attractive for imaging and fluorescence microscopy experiments...along with links to protocols, can be found below: SapTrap-SEC kit for C. elegans genome engineering - Bob...kit can be found at the Goldstein Lab website . SapTrap CRISPR/Cas Toolkit - Erik Jorgensen Lab. A collection...using CRISPR/Cas9 and the selection of C. elegans strains. CRISPR plasmids from John Calarco's lab as described...Craig Mello's lab and described in A Co-CRISPR Strategy for Efficient Genome Editing in Caenorhabditis...

...NanoLuc fusions are useful for both intracellular and extracellular applications where you want minimal...HaloTag, SmBiT, and LgBiT, useful for detecting and tracking key drug target proteins. Depositor... educational resources, and efficient material transfer solutions that help advance science while reducing...analysis and protein fusion vectors for detecting and tracking proteins using tags such as NanoLuc, HaloTag, ...Promega collection are available in two formats: pretransformed in bacteria as an agar stab 20 µg purified ...signaling pathways, investigating RNA regulation, tracking protein degradation, analyzing CRISPR-mediated...bright luminescent signal in the presence of a substrate. NanoBiT PPI assays enable kinetic, real-time ...

...Vector Core Viral Vectors Lentiviral Transfer Vectors Adenoviral Transfer Vectors Resources Kazuhiro Oka and...lentiviral and adenoviral transfer vectors with Addgene. Lentiviral Transfer Vectors Within this set of...packaging these transfer vectors and generating new viral vectors. Download the Lentivirus Transfer Vector Production...from the PGK promoter Adenoviral Transfer Vectors The adenoviral transfer vectors contained within this ... of lentiviral transfer vectors you can find reporter plasmids to test whether or not you've efficiently...coexpressed with tdTomato and Pac (puromycin N-acethyl-transferase) pCDH-CB-copGFP-T2A-iCre 72256 Expresses copGFP...Flp recombinases. Genes can be cloned into these transfer vectors using shuttle vectors pICPIS-EF1 (Addgene...

...Browse the Zhang lab’s comprehensive human transcription factor collection, available at Addgene. The...The collection can be used for screening transcription factors for cellular programming or cancer drug ...Library The MORF library is a comprehensive human transcription factor collection that contains 1,836 genes ...collection, can be purchased at Addgene. Each transcription factor isoform has a unique 24 bp barcode that...that simplifies identification of the transcription factor isoform in pooled screens. This collection ...collection and library can be used for screening transcription factors for applications such as cellular programming...cancer drug resistance. Protocols & References A transcription factor atlas of directed differentiation. Joung...

... to strain-specific tropism (See S3 table (Huang, et al., 2019) for LY6A-permissive mouse strains). AAV-PHP.eC... not display strain-specific tropism and can be used in LY6A-nonpermissive mouse strains. **AAV1-X1 has....AX with GRE or transgenic animal Oligodendrocytes - AAV-PHP.eB with GRE or transgenic animal Brain vascular....AX with GRE or transgenic animal L5 & Inhibitory - AAV-PHP.eB with GRE or transgenic animal L2/3 & L4...AAV.CAP-B10 with GRE or transgenic animal Excitatory - AAV.PHP.N with GRE or transgenic animal For PNS applications...several factors including: the delivery method, mouse strain, target cell type or tissue of interest, and model...

... Dorus Gadella 58473 pEGFP-C1 F-tractin-EGFP Actin filaments F-tractin EGFP Dyche Mullins 41147 pEGFP ...Atsushi Miyawaki 212019 pcDNA3/F-tractin=mStayGold Actin filaments F-tractin mStayGold Atsushi Miyawaki 229608...Membrane Trafficking The secretory pathway is responsible for delivering proteins to the extracellular environment... Proteins are first translated at the endoplasmic reticulum, then are transported to the Golgi apparatus...Localization General Cytoskeleton Junctions Membrane Trafficking Additional Resources The subcellular localization...observe cytoskeleton remodeling or blocked membrane traffic. Addgene has assembled a collection of popular ...find markers for components of these membrane trafficking pathways and structures. ID Plasmid Structure...

...of the applicable transfer plasmid. We will help you gain legal usage of the transfer plasmid from the ...and useful gene delivery tools that effectively transduce many mammalian cell types. AAV is used in a wide...service lets scientists choose from over 4,000 transfer plasmids to be packaged in their choice of seven...chemogenetic, optogenetic, recombinase, and biosensor transfer plasmids in our repository are eligible for this...aliquots 1.0 mL Large 25 × 100 µL aliquots 2.5 mL Extra Large 50 × 100 µL aliquots 5.0 mL *AAV2 yields are...several important steps: MTA facilitation for the transfer plasmid, including legal approval and an implementation...experiments. Shipping 2–7 days We will email you with tracking information as soon as your order ships. Shipping...

...pcDNA3-HtrA2-EGFP HTRA2 GFP CMV Parkinson's L. Miguel Martins 14122 pcDNA3-HtrA2-EGFP S306A HTRA2 GFP CMV...pcDNA3-delta133HtrA-EGFP HTRA2 GFP CMV Parkinson's L. Miguel Martins 14126 pET20b-delta133HtrA2 HTRA2 His...pcDNA3-HtrA2-FLAG HTRA2 Flag CMV Parkinson's L. Miguel Martins 15939 pcDNA3-HtrA2-FLAG S142D HTRA2 Flag ...pcDNA3-HtrA2-FLAG S400D HTRA2 Flag CMV Parkinson's L. Miguel Martins 16153 pET20b-delta133HtrA2 S142A ...S142A HTRA2 His T7 Parkinson's L. Miguel Martins 16154 pET20b-delta133HtrA2 S142D HTRA2 His T7 Parkinson's...pET20b-delta133HtrA2 S400A HTRA2 His T7 Parkinson's L. Miguel Martins 16156 pET20b-delta133HtrA2 S400D ...S400D HTRA2 His T7 Parkinson's L. Miguel Martins 16157 pET20b-delta133HtrA2 A141S HTRA2 His T7 Parkinson's...

...into human FBL locus Jorgensen Lab SapTrap CRISPR/Cas Toolkit SapTrap is a modular toolkit from Erik Jorgensen's...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA... for improved efficiency and throughput over traditional tagging methods. Several different tagging techniques...CRISPR-Cas tagging system were tested by tagging transcription factors with FLAG in human cell lines. To repeat...insert genetic tags in the C. elegans genome. The SapTrap reaction produces a single plasmid targeting vector...vector that encodes both a guide RNA transcript and a repair template for an individual tagging event....26 plasmids; 21 of the plasmids are for use in SapTrap reactions for targeting vector assembly, and the...

... bind transcriptional co-activators and modulate transcription. The list of p53 transcriptional targets...collection of plasmids for the p53 pathway. p53 is a transcription factor and tumor suppressor activated by cellular...p53 Gene List References Background p53 is a transcription factor and tumor suppressor activated by cellular...mutations interfere with p53’s ability to activate transcription, and they also have a dominant negative effect...page are provided below. Symbol Name 14-3-3-σ Stratifin Apaf-1 Apoptotic peptidase activating factor 1... receptor superfamily, member 10b E2F-1 E2F transcription factor 1 Fas Fas cell surface death receptor...

...(COVID-19) pandemic was caused by a novel virus strain from the Coronaviridae family called SARS-CoV-2...used for measuring viral entry and activity in neutralization assays and links to popular envelope and packaging...SARS-CoV-2-Human Interaction Network Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV...Anderson et al., 2020). BSG plasmids - (CD147), transmembrane glycoprotein of the immunoglobulin superfamily... protease (Mpro) activity reporters Reverse transcriptase, protease, and polymerase constructs for SARS-CoV...SARS-CoV-2 detection assays Neutralizing nanobody plasmids And more! ID Plasmid Description Industry PI...opens in a new window) Addgene's Protocol for RNA Extraction Without A Kit Bloom lab - Protocol and Reagents...

... the double-stranded siRNA and degrades one of the strands. The remaining single-stranded siRNA then binds...silencing is achieved through the delivery of a double-stranded RNA (dsRNA) that matches the mRNA target sequence...short interfering RNA). Delivery can occur via transfection or viral delivery of a plasmid. shRNAs are often...vector is taken up by a host, the viral genome is transcribed, producing the shRNA. Dicer, an RNase III enzyme... mechanism, therapeutic targets, and delivery strategies for cancer therapy . International Journal of...

...incorporated into the DNA by reverse transcription. The edited DNA strand displaces the unedited 5’ flap and...replace” gene editing method in which a reverse transcriptase (RT) is fused to the C terminus of Cas9 H840A...the target sequence where it nicks the non-target strand and generates a 3’ flap. The 3’ flap binds to the...sequence remains unchanged and available as a substrate for another round. Figure 1: Overview of prime...Moloney murine leukemia virus (M-MLV) reverse transcriptase. Engineering of the PE enzyme and related components...including exploring new sources for the reverse transcriptase. Many PE enzymes and related tools are available...

...deposition with APOE2 constructs . (Hudry et al. Sci Transl Med. 2013) Express human DNMT1 with a Myc tag. ... to using plasmid pooled libraries . Use the ultracompact and programmable endonuclease, TnpBmax , for...delivery of viral particles that will target the central nervous system, peripheral nervous system, vesicular...vesicular brain cells and more. Measure synaptic transmission with improved glutamate indicators . (Aggarwal...Differentiation Factors Find plasmids used to transform iPSCs and fibroblasts into neurons and more. Convert...plasmids expressing SOX10, OLIG2, and NKX6.2 transcription factors for rapid and efficient generation of...astrocytes to dopaminergic neurons using three transcription factors in a single polycistronic lentiviral...

...Spagehetti Monster, Brainbow Molecular Tools Tetracycline Transactivators, Affinity Purification, Cell Ablation...and useful gene delivery tools that effectively transduce many mammalian cell types. AAV is used in a wide... Packaged on Request service. You can choose a transfer plasmid from our repository and request packaging...Ablation, CRISPR Monosynaptic Neuronal Tracing Rabies Virus-Based Serotype Serotype Testing Samples Small samples...Testing Panel for the Eye and Brain For enhanced transduction of the eye and other tissues All Serotypes If...

...may yield slightly different DNA concentrations. Can I directly transfect cloning grade DNA into cells? ...experiments by removing the amplification and extraction steps required when one receives plasmids in ...DNA Service Details Amount 2 µg Volume 20 µl Concentration 100 ng/µl (± 5 ng/µl) Buffer 10 mM Tris-Cl, ...validated by next generation sequencing. The DNA concentration and 260/280 ratio has been determined by spectrophotometer...designed for use in cloning applications. Direct transfection into cells is not recommended. Can I order more...

...generate double strand breaks (DSBs). Nick Mutated Cas nickase enzymes generate single-strand DNA breaks. ...deletions. Insert CRISPR transposases insert large fragments of DNA. Transcriptional Regulation Activate dCas9...Prime Edit Cas nickase enzymes fused to reverse transcriptase install new genetic information into a specific..., or fused to a repressor peptide, inhibits transcription. Epigenetics dCas9 fused to an epigenetic modifier...

...124606 SOX2-mEGFP AICSDP-60 mEGFP Transcription factor SOX-2 Transcription Factor 124607 ACTN2-mEGFP AICSDP...Microtubules 101786 ST6GAL1-mEGFP AICSDP-26 mEGFP Sialyltransferase 1 Golgi 109122 NPM1-mEGFP AICSDP-50 mEGFP ...133963 UBTF-HaloTag AICSDP-80 HaloTag Nucleolar transcription factor UBF Nucleolus (fibrillar center) 133964...159745 TFAM-mEGFP AICSDP-72 mEGFP Mitochondrial transcription factor A (TFAM) Mitochondrial nucleoids 164499...human iPS cells (Link opens in a new window) RNP transfection for gene editing human iPS cells (Link opens...

...mutations. Resources New Tool for Lineage Tracing: The ClonTracer Library (Frank Stegmeier) Tackling Cancers...kinase that anchors a signaling network for transitioning between anabolic and catabolic states. Ras Ras...frequently mutated gene in human cancer, is a transcription factor and tumor suppressor. Wnt Coming soon...cDNAs for studying oncogenic signaling pathways. ClonTracer Library : Barcoding library from the Stegmeier...

...21871 pMLM36 (aka pBR-UV5-GP-FD2) 21874 Strain KJ1C 21875 Strain CSH100 Content blocked, you may need to... Keith Joung This kit consists of plasmids and strains used to engineer ZFNs via the Oligomerized Pool... packaged plasmids and 3 individually packaged strains, which are shipped as bacterial stabs at room temperature... pAC-Kan-alphaGal4 13422 pBAC-lacZ 13424 KJBAC1 strain 21869 pKJ1267 (aka pAC-alphaGal4) 21870 pKJ1712...