...MECP2 disorders. The RSRT has a comprehensive, strategic and aggressive plan to use gene editing, MECP2...reactivation, RNA editing, gene replacement therapy, RNA trans-splicing, and protein replacement to reverse the...purposeful hand movements and regression of speech. In contrast to the sustained loss of skills in neurodegenerative...disease severity. The MECP2 protein is a global transcriptional regulator of thousands of genes and studies...studies have suggested roles in transcriptional repression, activity dependent de-repression, chromatin remodeling...DNA and recruit known co-repressors or other transcriptional modifiers suggests the major function of MECP2...missense mutations cluster in the MBD and NID demonstrating the importance of binding to methylated DNA ...

... E2F E2F1 E2F2 E2F3 E2F transcription factor ECT2 Epithelial cell transforming 2 EGFR Epidermal growth...family. GTPases serve as molecular switches that transmit signals within the cell. GTPases have an active...EIF4EBP EIF4EBP1 EIF4EBP2 EIF4EBP3 Eukaryotic translation initiation factor 4E binding protein ERBB2 Erb-b2...protein kinase ETS ETS1 ETS2 ETS proto-oncogene, transcription factor EXOC EXOC1 EXOC2 EXOC3 EXOC4 EXOC5 EXOC6... related tyrosine kinase 3 FNT FNTA FNTB Farnesyltransferase, CAAX box FOS FBJ murine osteosarcoma viral...protein 2 ICMT Isoprenylcysteine carboxyl methyltransferase INSR Insulin receptor INSRR Insulin receptor-related...receptor-related receptor IRS IRS1 IRS2 Insulin receptor substrate JUN Jun proto-oncogene KSR KSR1 KSR2 Kinase suppressor...

... Flp, here expressed through a double transgenic animal strategy (PV-2a-Cre; SOM-IRES-Flp). Image from...recombinase-expression strategies to define cellular sub-populations of interest, including dual-transgenic recombinase-expressing...Ansorge M, Awatramani R, Kang UJ, Rayport S. 2018. Dopamine neuron glutamate cotransmission evokes a delayed... AND Flp, Cre NOT Flp, or Flp NOT Cre. B) This strategy has been expanded to incorporate VCre in order... targeting) is a synthetic molecular targeting strategy that allows adeno-associated virus (AAV)-borne...recombinase-expressing mouse lines 2–9 and combinations of transgenic recombinase-expressing animal lines and retro-grade...in a new window) of Flp-recombinase-expressing transgenic mouse and rat lines. The Fenno Lab maintains ...

...in vitro transcription as an alternative. Based on transient double-selection of transfected cells, the...new tools for genome engineering. Fusions of transcription activator-like (TAL) effectors of plant pathogenic...comprised of 834 plasmids of pre-assembled trimer and tetramer RVD domains designed for rapid TALEN assembly....and Chad Cowan Ekker Lab TALEN Kit All possible tetramers (264) of RVDs, compatible with Golden Gate TALEN...strong nuclear localisation tags and repress transcription initiation when targeted to DNA sequences within...Gate-assembled TALEN, both in microinjected embryos and transfected cells. TALEN expression is driven by the strong...reporter vector pGL4-SSA can be used for single-strand annealing assay, which enables the evaluation of...

...editing to easily engineer specific transgenic worm lines. The transparent body of the C. elegans hermaphrodite...Caenorhabditis elegans. Mos1 Based Transgenesis miniMos Transgenesis Kit - Erik Jorgensen Lab. This kit...consists of 959 somatic cells, which makes them attractive for imaging and fluorescence microscopy experiments...along with links to protocols, can be found below: SapTrap-SEC kit for C. elegans genome engineering - Bob...kit can be found at the Goldstein Lab website . SapTrap CRISPR/Cas Toolkit - Erik Jorgensen Lab. A collection...using CRISPR/Cas9 and the selection of C. elegans strains. CRISPR plasmids from John Calarco's lab as described...Craig Mello's lab and described in A Co-CRISPR Strategy for Efficient Genome Editing in Caenorhabditis...

... (COVID-19) pandemic is caused by a novel virus strain from the Coronaviridae family called SARS-CoV-2... Plasmids expressing Cas12a/Cas13a, Reverse Transcriptases, etc. that may be useful for detection assays...used for measuring viral entry and activity in neutralization assays and links to popular envelope and packaging...site for furin proteases.( 3 ) BSG - (CD147), transmembrane glycoprotein of the immunoglobulin superfamily... method termed DNA Endonuclease Targeted CRISPR Trans Reporter (DETECTR). Mammoth Biosciences has published...2020.06.23.166397 (Link opens in a new window) reverse transcriptase, protease, and Bst polymerase constructs now...now available from Andrea Pauli's lab. Neutralizing nanobodies bind SARS-CoV-2 spike RBD and block interaction...

...lentiviral and adenoviral transfer vectors with Addgene. Lentiviral Transfer Vectors Within this set of...packaging these transfer vectors and generating infectious virus. Protocol: Lentivirus Transfer Vector Production...Plasmid FAQs Biosafety Resource Guide Lentiviral Transfer Vectors Adenoviral Vectors Kazuhiro Oka and the... of lentiviral transfer vectors you can find reporter plasmids to test whether or not you've efficiently...coexpressed with tdTomato and Pac (puromycin N-acethyl-transferase) pCDH-CB-copGFP-T2A-iCre 72256 Expresses copGFP...PGK promoter Adenoviral Vectors The adenoviral transfer vectors contained within this set are derived ...Flp recombinases. Genes can be cloned into these transfer vectors using shuttle vectors pICPIS-EF1 and pICPIS-CB...

...Browse the Zhang lab’s comprehensive human transcription factor collection, available at Addgene. The...The collection can be used for screening transcription factors for cellular programming or cancer drug ...Library The MORF library is a comprehensive human transcription factor collection that contains 1,836 genes ...collection, can be purchased at Addgene. Each transcription factor isoform has a unique 24 bp barcode that...that simplifies identification of the transcription factor isoform in pooled screens. This collection ...collection and library can be used for screening transcription factors for applications such as cellular programming...cancer drug resistance. Protocols & References A transcription factor atlas of directed differentiation. Joung...

... to strain-specific tropism (See S3 table (Huang, et al., 2019) for LY6A-permissive mouse strains). AAV-PHP.eC... not display strain-specific tropism and can be used in LY6A-nonpermissive mouse strains. **AAV1-X1 has....AX with GRE or transgenic animal Oligodendrocytes - AAV-PHP.eB with GRE or transgenic animal Brain vascular....AX with GRE or transgenic animal L5 & Inhibitory - AAV-PHP.eB with GRE or transgenic animal L2/3 & L4...AAV.CAP-B10 with GRE or transgenic animal Excitatory - AAV.PHP.N with GRE or transgenic animal For PNS applications...several factors including: the delivery method, mouse strain, target cell type or tissue of interest, and model...

...pcDNA3-HtrA2-EGFP HTRA2 GFP CMV Parkinson's L. Miguel Martins 14122 pcDNA3-HtrA2-EGFP S306A HTRA2 GFP CMV...pcDNA3-delta133HtrA-EGFP HTRA2 GFP CMV Parkinson's L. Miguel Martins 14126 pET20b-delta133HtrA2 HTRA2 His...pcDNA3-HtrA2-FLAG HTRA2 Flag CMV Parkinson's L. Miguel Martins 15939 pcDNA3-HtrA2-FLAG S142D HTRA2 Flag ...pcDNA3-HtrA2-FLAG S400D HTRA2 Flag CMV Parkinson's L. Miguel Martins 16153 pET20b-delta133HtrA2 S142A ...S142A HTRA2 His T7 Parkinson's L. Miguel Martins 16154 pET20b-delta133HtrA2 S142D HTRA2 His T7 Parkinson's...pET20b-delta133HtrA2 S400A HTRA2 His T7 Parkinson's L. Miguel Martins 16156 pET20b-delta133HtrA2 S400D ...S400D HTRA2 His T7 Parkinson's L. Miguel Martins 16157 pET20b-delta133HtrA2 A141S HTRA2 His T7 Parkinson's...

...dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. ...(see paper) Transfection none, need Cas9 plasmid Calarco 47943 pMB70 cU6 BsaI Transfection none, need ...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...functional CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location based on a gRNA-defined... cut Goldstein 42250 DR274 T7 BsaI In vitro transcription none, need Cas9 plasmid Joung 46170 PU6::klp... plasmid Boxem 47941 pMB60 T7 BsaI In vitro transcription none, need Cas9 plasmid Boxem 47912 SP6-sgRNA-scaffold...sgRNA-scaffold SP6 AflII In vitro transcription none, need Cas9 plasmid Sternberg 177783 L4440_BioBrick-...

...are monomers at low concentration but are prone to dimerization at high concentration, so this is noted ...eqFP611 559 611 35 Tetramer eqFP611-N1 - Mammalian Expression DsRed2 563 582 24 Tetramer pCAG-DsRed2 - Mammalian... - Mammalian Expression mStrawberry 574 596 26 4.5 50 min Monomer mStrawberry-N1 - Mammalian Expression...contain the A206K mutation are monomeric at all concentrations, so this is mutation is noted when present....CYPet-C1 - Mammalian Expression AmCyan1 453 486 11 Tetramer AmCyan1-N1 - Mammalian Expression MiCy (Midoriishi-Cyan...Expression (cysteine-free SGFP2) ZsGreen 493 505 39 Tetramer pHIV-Zsgreen - Mammalian Expression (this is a...YPet-pBAD - Bacterial Expression ZsYellow1 529 539 8 Tetramer ZsYellow1-N1 - Mammalian Expression mPapaya1 530...

...resonance energy transfer, or FRET , is a process by which energy is non-radiatively transferred from an excited...excited donor fluorophore to an acceptor. Since the transfer of energy does not occur by emission of a photon...FRET experiments. The efficiency of the energy transfer is measured using fluorescence microscopy by exciting...fused to the same protein (also referred to as intramolecular or unimolecular FRET). Fluorescent proteins...-5aa linker-Cerulean-6aa linker-Venus ACAV Heterotetrameric construct consisting of Amber-5aa linker-Cerulean...linker-Cerulean-5aa linker-Amber-6aa linker-Venus ACVA Heterotetrameric construct consisting of Amber-5aa linker-Cerulean...linker-Cerulean-5aa linker-Venus-6aa linker-Amber VCAA Heterotetrameric construct consisting of Venus-5aa linker-Cerulean...

...into human FBL locus Jorgensen Lab SapTrap CRISPR/Cas Toolkit SapTrap is a modular toolkit from Erik Jorgensen's...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA... for improved efficiency and throughput over traditional tagging methods. Several different tagging techniques...CRISPR-Cas tagging system were tested by tagging transcription factors with FLAG in human cell lines. To repeat...insert genetic tags in the C. elegans genome. The SapTrap reaction produces a single plasmid targeting vector...vector that encodes both a guide RNA transcript and a repair template for an individual tagging event....26 plasmids; 21 of the plasmids are for use in SapTrap reactions for targeting vector assembly, and the...

...incorporated into the DNA by reverse transcription. The edited DNA strand displaces the unedited 5’ flap and...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...replace” gene editing method in which a reverse transcriptase (RT) is fused to the C terminus of Cas9 H840A...the target sequence where it nicks the non-target strand and generates a 3’ flap. The 3’ flap binds to the...sequence remains unchanged and available as a substrate for another round. Figure 1: Overview of prime...Moloney murine leukemia virus (M-MLV) reverse transcriptase. Engineering of the PE enzyme and related components...including exploring new sources for the reverse transcriptase. Many PE enzymes and related tools are available...

...of the applicable transfer plasmid. We will help you gain legal usage of the transfer plasmid from the ...and useful gene delivery tools that effectively transduce many mammalian cell types. AAV is used in a wide...service lets scientists choose from over 3,000 transfer plasmids to be packaged in their choice of five...chemogenetic, optogenetic, recombinase, and biosensor transfer plasmids in our repository are eligible for this...several important steps: MTA facilitation for the transfer plasmid, including legal approval and an implementation...experiments. Shipping 2–7 days We will email you with tracking information as soon as your order ships. Shipping...vector preps at Addgene, including purification, titration, and quality control measures. We have distributed...

... bind transcriptional co-activators and modulate transcription. The list of p53 transcriptional targets...collection of plasmids for the p53 pathway. p53 is a transcription factor and tumor suppressor activated by cellular...p53 Gene List References Background p53 is a transcription factor and tumor suppressor activated by cellular...mutations interfere with p53’s ability to activate transcription, and they also have a dominant negative effect...page are provided below. Symbol Name 14-3-3-σ Stratifin Apaf-1 Apoptotic peptidase activating factor 1... receptor superfamily, member 10b E2F-1 E2F transcription factor 1 Fas Fas cell surface death receptor...

... the double-stranded siRNA and degrades one of the strands. The remaining single-stranded siRNA then binds...silencing is achieved through the delivery of a double-stranded RNA (dsRNA) that matches the mRNA target sequence...short interfering RNA). Delivery can occur via transfection or viral delivery of a plasmid. shRNAs are often...vector is taken up by a host, the viral genome is transcribed, producing the shRNA. Dicer, an RNase III enzyme... mechanism, therapeutic targets, and delivery strategies for cancer therapy . International Journal of...

... Dorus Gadella 58473 pEGFP-C1 F-tractin-EGFP Actin Filaments F-tractin EGFP Dyche Mullins 41147 pEGFP ...proteins from the cell to the extracellular environment. Proteins are first translated at the endoplasmic reticulum...Caveolae Cav1 mCherry Ari Helenius 37533 cfSGFP2-N Extracellular milieu cfSGFP2-N EGFP Ikuo Wada 73208 pcDNA3.1...2XG4S-mCer3-SKL Peroxisome SKL tripeptide, peroxisome transport signal dL5 FAP; mCerulean3 Marcel Bruchez 85065...reticulum, then are transported to the Golgi apparatus in vesicles to be packaged and shipped. Many of ...require chaperones to help with folding or post-translational modification to function properly. Browse or...GalToxBFP Golgi apparatus GalT signal anchor transmembrane domain oxBFP Erik Snapp 158005 pCMV-mGold-MannII-N...

...Spagehetti Monster, Brainbow Molecular Tools Tetracycline Transactivators, Affinity Purification, Cell Ablation...and useful gene delivery tools that effectively transduce many mammalian cell types. AAV is used in a wide... Packaged on Request service. You can choose a transfer plasmid from our repository and request packaging...Ablation Monosynaptic Neuronal Tracing Rabies Virus-Based Serotype Serotype Testing Samples Small samples of...Testing Panel for the Eye and Brain For enhanced transduction of the eye and other tissues All Serotypes If...