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We narrowed to 123 results for: tra

Showing: 41 - 60 of 123 results

  1. Rett Syndrome

    Type
    Collection
    ...MECP2 disorders. The RSRT has a comprehensive, strategic and aggressive plan to use gene editing, MECP2...reactivation, RNA editing, gene replacement therapy, RNA trans-splicing, and protein replacement to reverse the...purposeful hand movements and regression of speech. In contrast to the sustained loss of skills in neurodegenerative...disease severity. The MECP2 protein is a global transcriptional regulator of thousands of genes and studies...studies have suggested roles in transcriptional repression, activity dependent de-repression, chromatin remodeling...DNA and recruit known co-repressors or other transcriptional modifiers suggests the major function of MECP2...missense mutations cluster in the MBD and NID demonstrating the importance of binding to methylated DNA ...

  2. Ras Pathway

    Type
    Collection
    ... E2F E2F1 E2F2 E2F3 E2F transcription factor ECT2 Epithelial cell transforming 2 EGFR Epidermal growth...family. GTPases serve as molecular switches that transmit signals within the cell. GTPases have an active...EIF4EBP EIF4EBP1 EIF4EBP2 EIF4EBP3 Eukaryotic translation initiation factor 4E binding protein ERBB2 Erb-b2...protein kinase ETS ETS1 ETS2 ETS proto-oncogene, transcription factor EXOC EXOC1 EXOC2 EXOC3 EXOC4 EXOC5 EXOC6... related tyrosine kinase 3 FNT FNTA FNTB Farnesyltransferase, CAAX box FOS FBJ murine osteosarcoma viral...protein 2 ICMT Isoprenylcysteine carboxyl methyltransferase INSR Insulin receptor INSRR Insulin receptor-related...receptor-related receptor IRS IRS1 IRS2 Insulin receptor substrate JUN Jun proto-oncogene KSR KSR1 KSR2 Kinase suppressor...

  3. Deisseroth INTRSECT Collection

    Type
    Collection
    ... Flp, here expressed through a double transgenic animal strategy (PV-2a-Cre; SOM-IRES-Flp). Image from...recombinase-expression strategies to define cellular sub-populations of interest, including dual-transgenic recombinase-expressing...Ansorge M, Awatramani R, Kang UJ, Rayport S. 2018. Dopamine neuron glutamate cotransmission evokes a delayed... AND Flp, Cre NOT Flp, or Flp NOT Cre. B) This strategy has been expanded to incorporate VCre in order... targeting) is a synthetic molecular targeting strategy that allows adeno-associated virus (AAV)-borne...recombinase-expressing mouse lines 2–9 and combinations of transgenic recombinase-expressing animal lines and retro-grade...in a new window) of Flp-recombinase-expressing transgenic mouse and rat lines. The Fenno Lab maintains ...

  4. TALEN Plasmids and Kits

    Type
    Collection
    ...in vitro transcription as an alternative. Based on transient double-selection of transfected cells, the...new tools for genome engineering. Fusions of transcription activator-like (TAL) effectors of plant pathogenic...comprised of 834 plasmids of pre-assembled trimer and tetramer RVD domains designed for rapid TALEN assembly....and Chad Cowan Ekker Lab TALEN Kit All possible tetramers (264) of RVDs, compatible with Golden Gate TALEN...strong nuclear localisation tags and repress transcription initiation when targeted to DNA sequences within...Gate-assembled TALEN, both in microinjected embryos and transfected cells. TALEN expression is driven by the strong...reporter vector pGL4-SSA can be used for single-strand annealing assay, which enables the evaluation of...

  5. Worm Expression Resources

    Type
    Collection
    ...editing to easily engineer specific transgenic worm lines. The transparent body of the C. elegans hermaphrodite...Caenorhabditis elegans. Mos1 Based Transgenesis miniMos Transgenesis Kit - Erik Jorgensen Lab. This kit...consists of 959 somatic cells, which makes them attractive for imaging and fluorescence microscopy experiments...along with links to protocols, can be found below: SapTrap-SEC kit for C. elegans genome engineering - Bob...kit can be found at the Goldstein Lab website . SapTrap CRISPR/Cas Toolkit - Erik Jorgensen Lab. A collection...using CRISPR/Cas9 and the selection of C. elegans strains. CRISPR plasmids from John Calarco's lab as described...Craig Mello's lab and described in A Co-CRISPR Strategy for Efficient Genome Editing in Caenorhabditis...

  6. Promega Plasmid Collection

    Type
    Collection
    ...NanoLuc fusions are useful for both intracellular and extracellular applications where you want minimal...HaloTag, SmBiT, and LgBiT, useful for detecting and tracking key drug target proteins. Depositor... educational resources, and efficient material transfer solutions that help advance science while reducing...analysis and protein fusion vectors for detecting and tracking proteins using tags such as NanoLuc, HaloTag, ...Promega collection are available in two formats: pretransformed in bacteria as an agar stab 20 µg purified ...signaling pathways, investigating RNA regulation, tracking protein degradation, analyzing CRISPR-mediated...bright luminescent signal in the presence of a substrate. NanoBiT PPI assays enable kinetic, real-time ...

  7. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...Vector Core Viral Vectors Lentiviral Transfer Vectors Adenoviral Transfer Vectors Resources Kazuhiro Oka and...lentiviral and adenoviral transfer vectors with Addgene. Lentiviral Transfer Vectors Within this set of...packaging these transfer vectors and generating new viral vectors. Download the Lentivirus Transfer Vector Production...from the PGK promoter Adenoviral Transfer Vectors The adenoviral transfer vectors contained within this ... of lentiviral transfer vectors you can find reporter plasmids to test whether or not you've efficiently...coexpressed with tdTomato and Pac (puromycin N-acethyl-transferase) pCDH-CB-copGFP-T2A-iCre 72256 Expresses copGFP...Flp recombinases. Genes can be cloned into these transfer vectors using shuttle vectors pICPIS-EF1 (Addgene...

  8. Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection

    Type
    Collection
    ...Browse the Zhang lab’s comprehensive human transcription factor collection, available at Addgene. The...The collection can be used for screening transcription factors for cellular programming or cancer drug ...Library The MORF library is a comprehensive human transcription factor collection that contains 1,836 genes ...collection, can be purchased at Addgene. Each transcription factor isoform has a unique 24 bp barcode that...that simplifies identification of the transcription factor isoform in pooled screens. This collection ...collection and library can be used for screening transcription factors for applications such as cellular programming...cancer drug resistance. Protocols & References A transcription factor atlas of directed differentiation. Joung...

  9. Caltech Systemic Capsids

    Type
    Collection
    ... to strain-specific tropism (See S3 table (Huang, et al., 2019) for LY6A-permissive mouse strains). AAV-PHP.eC... not display strain-specific tropism and can be used in LY6A-nonpermissive mouse strains. **AAV1-X1 has....AX with GRE or transgenic animal Oligodendrocytes - AAV-PHP.eB with GRE or transgenic animal Brain vascular....AX with GRE or transgenic animal L5 & Inhibitory - AAV-PHP.eB with GRE or transgenic animal L2/3 & L4...AAV.CAP-B10 with GRE or transgenic animal Excitatory - AAV.PHP.N with GRE or transgenic animal For PNS applications...several factors including: the delivery method, mouse strain, target cell type or tissue of interest, and model...

  10. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. ...(see paper) Transfection none, need Cas9 plasmid Calarco 47943 pMB70 cU6 BsaI Transfection none, need ...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...functional CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location based on a gRNA-defined... cut Goldstein 42250 DR274 T7 BsaI In vitro transcription none, need Cas9 plasmid Joung 46170 PU6::klp... plasmid Boxem 47941 pMB60 T7 BsaI In vitro transcription none, need Cas9 plasmid Boxem 47912 SP6-sgRNA-scaffold...sgRNA-scaffold SP6 AflII In vitro transcription none, need Cas9 plasmid Sternberg 177783 L4440_BioBrick-...

  11. AAV Packaged on Request

    Type
    Collection
    ...of the applicable transfer plasmid. We will help you gain legal usage of the transfer plasmid from the ...and useful gene delivery tools that effectively transduce many mammalian cell types. AAV is used in a wide...service lets scientists choose from over 4,000 transfer plasmids to be packaged in their choice of seven...chemogenetic, optogenetic, recombinase, and biosensor transfer plasmids in our repository are eligible for this...aliquots 1.0 mL Large 25 × 100 µL aliquots 2.5 mL Extra Large 50 × 100 µL aliquots 5.0 mL *AAV2 yields are...several important steps: MTA facilitation for the transfer plasmid, including legal approval and an implementation...experiments. Shipping 2–7 days We will email you with tracking information as soon as your order ships. Shipping...

  12. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...pcDNA3-HtrA2-EGFP HTRA2 GFP CMV Parkinson's L. Miguel Martins 14122 pcDNA3-HtrA2-EGFP S306A HTRA2 GFP CMV...pcDNA3-delta133HtrA-EGFP HTRA2 GFP CMV Parkinson's L. Miguel Martins 14126 pET20b-delta133HtrA2 HTRA2 His...pcDNA3-HtrA2-FLAG HTRA2 Flag CMV Parkinson's L. Miguel Martins 15939 pcDNA3-HtrA2-FLAG S142D HTRA2 Flag ...pcDNA3-HtrA2-FLAG S400D HTRA2 Flag CMV Parkinson's L. Miguel Martins 16153 pET20b-delta133HtrA2 S142A ...S142A HTRA2 His T7 Parkinson's L. Miguel Martins 16154 pET20b-delta133HtrA2 S142D HTRA2 His T7 Parkinson's...pET20b-delta133HtrA2 S400A HTRA2 His T7 Parkinson's L. Miguel Martins 16156 pET20b-delta133HtrA2 S400D ...S400D HTRA2 His T7 Parkinson's L. Miguel Martins 16157 pET20b-delta133HtrA2 A141S HTRA2 His T7 Parkinson's...

  13. CRISPR Plasmids - Tagging

    Type
    Collection
    ...into human FBL locus Jorgensen Lab SapTrap CRISPR/Cas Toolkit SapTrap is a modular toolkit from Erik Jorgensen's...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA... for improved efficiency and throughput over traditional tagging methods. Several different tagging techniques...CRISPR-Cas tagging system were tested by tagging transcription factors with FLAG in human cell lines. To repeat...insert genetic tags in the C. elegans genome. The SapTrap reaction produces a single plasmid targeting vector...vector that encodes both a guide RNA transcript and a repair template for an individual tagging event....26 plasmids; 21 of the plasmids are for use in SapTrap reactions for targeting vector assembly, and the...

  14. p53 Pathway

    Type
    Collection
    ... bind transcriptional co-activators and modulate transcription. The list of p53 transcriptional targets...collection of plasmids for the p53 pathway. p53 is a transcription factor and tumor suppressor activated by cellular...p53 Gene List References Background p53 is a transcription factor and tumor suppressor activated by cellular...mutations interfere with p53’s ability to activate transcription, and they also have a dominant negative effect...page are provided below. Symbol Name 14-3-3-σ Stratifin Apaf-1 Apoptotic peptidase activating factor 1... receptor superfamily, member 10b E2F-1 E2F transcription factor 1 Fas Fas cell surface death receptor...

  15. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ... Dorus Gadella 58473 pEGFP-C1 F-tractin-EGFP Actin Filaments F-tractin EGFP Dyche Mullins 41147 pEGFP ...proteins from the cell to the extracellular environment. Proteins are first translated at the endoplasmic reticulum...remodeling the cytoskeleton or blocking membrane trafficking. Addgene has assembled a collection of fluorescent...Caveolae Cav1 mCherry Ari Helenius 37533 cfSGFP2-N Extracellular milieu cfSGFP2-N EGFP Ikuo Wada 73208 pcDNA3.1...2XG4S-mCer3-SKL Peroxisome SKL tripeptide, peroxisome transport signal dL5 FAP; mCerulean3 Marcel Bruchez 85065...reticulum, then are transported to the Golgi apparatus in vesicles to be packaged and shipped. Many of ...require chaperones to help with folding or post-translational modification to function properly. Browse or...

  16. COVID-19 Resources

    Type
    Collection
    ...(COVID-19) pandemic was caused by a novel virus strain from the Coronaviridae family called SARS-CoV-2...used for measuring viral entry and activity in neutralization assays and links to popular envelope and packaging...SARS-CoV-2-Human Interaction Network Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV...Anderson et al., 2020). BSG plasmids - (CD147), transmembrane glycoprotein of the immunoglobulin superfamily... protease (Mpro) activity reporters Reverse transcriptase, protease, and polymerase constructs for SARS-CoV...SARS-CoV-2 detection assays Neutralizing nanobody plasmids And more! ID Plasmid Description Industry PI...opens in a new window) Addgene's Protocol for RNA Extraction Without A Kit Bloom lab - Protocol and Reagents...

  17. Mammalian RNAi Tools

    Type
    Collection
    ... the double-stranded siRNA and degrades one of the strands. The remaining single-stranded siRNA then binds...silencing is achieved through the delivery of a double-stranded RNA (dsRNA) that matches the mRNA target sequence...short interfering RNA). Delivery can occur via transfection or viral delivery of a plasmid. shRNAs are often...vector is taken up by a host, the viral genome is transcribed, producing the shRNA. Dicer, an RNase III enzyme... mechanism, therapeutic targets, and delivery strategies for cancer therapy . International Journal of...

  18. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...incorporated into the DNA by reverse transcription. The edited DNA strand displaces the unedited 5’ flap and...replace” gene editing method in which a reverse transcriptase (RT) is fused to the C terminus of Cas9 H840A...the target sequence where it nicks the non-target strand and generates a 3’ flap. The 3’ flap binds to the...sequence remains unchanged and available as a substrate for another round. Figure 1: Overview of prime...Moloney murine leukemia virus (M-MLV) reverse transcriptase. Engineering of the PE enzyme and related components...including exploring new sources for the reverse transcriptase. Many PE enzymes and related tools are available...

  19. AAV Viral Preps

    Type
    Collection
    ...Spagehetti Monster, Brainbow Molecular Tools Tetracycline Transactivators, Affinity Purification, Cell Ablation...and useful gene delivery tools that effectively transduce many mammalian cell types. AAV is used in a wide... Packaged on Request service. You can choose a transfer plasmid from our repository and request packaging...Ablation, CRISPR Monosynaptic Neuronal Tracing Rabies Virus-Based Serotype Serotype Testing Samples Small samples...Testing Panel for the Eye and Brain For enhanced transduction of the eye and other tissues All Serotypes If...

  20. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...may yield slightly different DNA concentrations. Can I directly transfect cloning grade DNA into cells? ...experiments by removing the amplification and extraction steps required when one receives plasmids in ...DNA Service Details Amount 2 µg Volume 20 µl Concentration 100 ng/µl (± 5 ng/µl) Buffer 10 mM Tris-Cl, ...validated by next generation sequencing. The DNA concentration and 260/280 ratio has been determined by spectrophotometer...designed for use in cloning applications. Direct transfection into cells is not recommended. Can I order more...
Showing: 41 - 60 of 123 results