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Showing: 61 - 80 of 124 results

  1. Genetic Code Expansion

    Type
    Collection
    ... or function in the protein can be observed. The introduced amino acid can also be used to intentionally... been introduced in the cells, the non-canonical amino acid can be incorporated using the existing protein...into the ribosome and if the anticodon on the tRNA can bind to the mRNA (hence, the anticodon is complementary...amino acid. Because there are no free codons, this can be challenging. In E.coli , the rarest codon is the...thus this codon is often used. The gene of interest can be expressed from a plasmid containing a UAG codon...from M. jannaschii , M. barkeri , or E.coli and can be mutated and screened through directed evolution...light. On a broader scale, the expanded genetic code can help us understand and evolve proteins for various...

  2. Luciferase Plasmid Collection

    Type
    Collection
    ...regulatory elements before or after the luciferase ORF can be found in a table below, as well as plasmids that...are driven by a strong constitutive promoter and can be used to monitor transfection or viral infection...collection of empty backbone plasmids into which you can clone your regulatory element or gene of interest... promoter/enhancer regions. Blue/white screening can be used for screening of transformants in cloning...Luciferase driven by a strong constitutive promoter and can be used to monitor transfection or viral infection...firefly luciferase and dTomato. A gene of interest can also be inserted into the plasmid. Malcolm Moore ...use the red-shifted substrate Akalumine with significantly brighter signal and emission in the near-infrared...

  3. Chemogenetics Plasmids

    Type
    Collection
    ...receptors are popular in neuroscience, where they can be used to interrogate brain function and examine...where the receptor is expressed, these receptors can be used to artificially activate or inhibit specific...

  4. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ... dCas9-VPR for activation. Usage Guide sequences can be designed using Benchling or using similar design...containing the guide target sequence: Up to two guides can be expressed from the same plasmid by utilizing one...

  5. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...Characterization of some of the FPs in this collection can be found in Botman et al., 2019 . FP Tagging Yeast.... The FP together with the protothropic cassette can be amplified with the following primers: FW 5'–GGTGACGGTGCTGGTTTA...

  6. Zebrafish Plasmid Collection

    Type
    Collection
    ...few examples of useful zebrafish CRISPR resources can be found below: UFlip CRISPR targeted integration...studying cells that share clonal origin in zebrafish can be found below: Optimized QF-binary expression system...in organisms or cells in which an exogenous mRNA can be injected and expressed. EXPRESs Plasmids for Zebrafish... translational research, including the study of cancer, inflammation and infection, regeneration, human... Zon Lab. A platform to rapidly model potential cancer drivers in vivo. Biotagging toolkit - Tatjana Sauka-Spengler...the international research community. The Latin American Zebrafish Network (LAZEN) - LAZEN aims to increase...

  7. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...the envelope surface. Pseudotyped virus particles can then be used to infect permissive cells that express...Plasmids A few examples of reporter plasmids that can be used for measuring infectivity and neutralizing...

  8. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...editors (CBEs) and adenine base editors (ABEs) — can be used to generate single base pair edits without...targeted to a specific locus by a gRNA, and they can convert cytidine to uridine within a small editing...

  9. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. Design...gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety of Cas-containing plasmids...

  10. Serotype Testing AAV

    Type
    Collection
    ...Testing AAV These AAV encode fluorescent reporters and can be used to compare the tropism of different serotypes...µL. The 20 µL option is an economical choice that can be used to validate the performance of various serotypes...

  11. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...serotype selection after you submit your request, so we can set expectations to the best of our knowledge from... three years of the order being shipped, Addgene can provide the QC validation data performed on the material...experiments carried out with our materials, Addgene cannot guarantee the function of any viral vector. Addgene...

  12. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...fusion protein using fluorescence microscopy, you can assess whether your protein is targeted to the same...for chemical reactions. Labeling these structures can be quite useful for identifying the localization ...domain tdTomato John Cooper *Fusions to other FPs can be found by searching the complete Michael Davidson...ensconsin mCherry William Bement *Fusions to other FPs can be found by searching the complete Michael Davidson...Occludens-1 GFP Alan Fanning *Fusions to other FPs can be found by searching the complete Michael Davidson...Rab11a DsRed Richard Pagano *Fusions to other FPs can be found by searching the complete Michael Davidson...

  13. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...does so; however, these independent gRNA plasmids can be paired with a wide variety of plasmids and therefore...CRISPR function. Alternatively, gRNA-only plasmids can be used in cells or organisms in which Cas9 has been...Addgene. To find the gRNA vector for your system, you can search or sort this table based on: Your particular...function of Cas9. We have plasmids with Cas9 that can cut, activate, interfere, or nick. Selection , such...to generate multiplex gRNA vectors. These systems can be used to express multiple gRNAs from a single plasmid...recombination-based cloning of complex polycistronic lentiviruses. Can express Cas9 with up to 3 gRNAs. Frew pSQT1313 Mammalian...module vectors, multiple gRNA expression cassettes can be assembled using Golden Gate cloning or the Gibson...

  14. COVID-19 Resources

    Type
    Collection
    ... at lightning speed to elucidate as much as they can about this novel virus. It is now known that SARS-CoV...COVID-19 Resources The following types of resources can be found on this page: SARS-CoV-2 Antibodies : Recombinant...libraries of Spike (S) Ectodomain and RBD mutants that can be used to identify antibody escape mutants and for...19 related articles spanning multiple publishers can be found here: ASM COVID-19 Research Registry COVID...-2 Spike Receptor Binding Domain Deep Mutation Scanning Library - Library of Spike protein RBD mutations...

  15. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...the video, you can find the protocol section from the original publication below. You can also download... the study of non-coding elements. This approach can be used for efficient loss-of-function studies of...protocol is for suspension cells. Adherent cells can either grow as individual cells in 96-well flat bottom...concentration so that individual single-cell derived clones can be picked and moved to a flat bottom 96-well plate...Mammalian Cell Lines via CRISPR/Cas9. Bauer DE, Canver MC, Orkin SH. J. Vis. Exp. (95), e52118, doi:10.3791...repeats (CRISPR)/Cas9 in mammalian cells. Canver MC, Bauer DE, Dass A, Yien YY, Chung J, Masuda ...

  16. Antibody Production

    Type
    Collection
    ...described below. Information for a specific antibody can be found on the antibody's catalog page or by contacting...conjugation. For applications such as these, the buffer can be removed by dialysis or gel filtration. Titer and...

  18. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...many plasmids have markers on them so that you can find or select for only the cells that received ...incorporated into the host DNA. For mammalian cells, you can do this through viral delivery. Visit our viral...Representative Empty Backbones Lentiviral Can transduce both dividing and nondividing ...

  19. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...creation of the hiPS cell lines The following plasmids can be used to create cell lines with endogenously-tagged...antigen (PCNA) DNA replication foci IPSC cell lines can be ordered from the Allen Institute for Cell Science...

  20. Brain Initiative Collection

    Type
    Collection
    ...cascades. Michael Bruchas 140135-AAV1 pAAV-EF1a-iCreV can be used to generate AAV virus that will express light-inducible...recombinase Hongkui Zeng 140135-PHPeB pAAV-EF1a-iCreV can be used to generate AAV virus that will express light-inducible...recombinase Hongkui Zeng 140136-AAV1 pAAV-EF1a-iDreV can be used to generate AAV virus that will expresslight-inducible...recombinase Hongkui Zeng 140136-PHPeB pAAV-EF1a-iDreV can be used to generate AAV virus that will expresslight-inducible...recombinase Hongkui Zeng 140137-AAV1 pAAV-EF1a-iFlpV can be used to generate AAV virus that will express light-inducible...recombinase Hongkui Zeng 140137-PHPeB pAAV-EF1a-iFlpV can be used to generate AAV virus that will express light-inducible...
Showing: 61 - 80 of 124 results