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Showing: 61 - 80 of 134 results

  1. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...pUC57-Simple-gRNA backbone T7 BsaI In vitro transcription none, need Cas9 plasmid Chen Do you have suggestions...

  2. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations,...

  3. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, PAM requirements, experimental...

  4. FlyCRISPR

    Type
    Collection
    ...in vivo transcrition controlled by the regulatory sequences of an RNA polymerase III transcribed U6 snRNA...non-model invertebrate species. These plasmids are described in: Genome engineering of Drosophila with the ...

  5. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations,...

  6. Jaenisch Lab CRISPR Plasmids

    Type
    Collection
    ...CRISPR Blog Posts CRISPR-on is an RNA-guided transcriptional activator system with two components, the dCas9...systems by LR clonase reaction. These plasmids are described in: Multiplexed activation of endogenous genes...genes by CRISPR-on, an RNA-guided transcriptional activator system. Cheng AW, Wang H, Yang H, Shi L, Katz...

  7. Adenovirus Plasmids

    Type
    Collection
    ...strains for generating adenovirus. ID Strain Description PI 16398 BJ5183 Strain for recombination between... plasmids that have inserts. ID Plasmid Type Description PI 16400 pAdEasy-1 Adenoviral For recombining... be assembled in a one-tube reaction. ID Kit Description PI 1000000176 AdenoBuilder toolkit Plasmids contain...

  8. Distribution to Industry

    Type
    Collection
    ...Plasmid Gene/Insert PI Kits Kit name Type PI Description High Complexity Golden Gate Assembly Standards...Pooled Libraries Pooled Library name Type PI Description HR700_TP53 Exon Mutation Libraries CRISPR Thorsten...exon 5–8. Phagemid Synuclein VHH Immune Library Screening Messer and MJFF Created for research in Parkinson's...

  9. Validated gRNA Sequences

    Type
    Collection
    ...the particular conditions of the experiment as described in the associated article (listed below by PubMed...within promoters. When possible, the categories described on Addgene's CRISPR Plasmids and Resources page...GCGGCAGCTCCTAGCTCAGC 61515 nick S. pyogenes 25569111 Hanna mitf S. scrofa CTTTCGGATATAATCCACGG 69801 cut S. pyogenes 26293209...AAAAAACCGAACTCCGCGCTGCGTAAAGTA 44505 cut S. pyogenes 23360965 Marraffini scramble synthetic AACCCCTGATTGTATCCGCA 62285 interfere...

  10. Brain Armamentarium

    Type
    Collection
    ...below. Plasmids ID Plasmid Description PI Viral Preps ID Viral Prep Description PI (Cargo) PI (Capsid) 214869...labeling in neurons. Useful for nuclear isolation and scRNA-seq applications. Jonathan Ting Viviana Gradinaru...

  11. Genetic Code Expansion

    Type
    Collection
    ...M. barkeri , or E.coli and can be mutated and screened through directed evolution to charge the tRNA ...research, you can get advice from GCE experts by subscribing to the GCE bulletin board (GCEbb) listserve and...non-standard amino acid incorporation. ID Strain Description PI 48998 C321.ΔA all TAG sites changes to UAG...

  12. Fluorescent Protein Guide: Activity Regulation

    Type
    Collection
    ...activity. Early tools allow scientists to regulate transcription, and additional tools are being developed to...Principal Investigator Plasmids GFP GFP-dependent transcription factors Connie Cepko See Plasmids Dronpa Optical...

  13. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...

  14. Synthetic Biology - Cloning and Genomic Tools

    Type
    Collection
    ...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Back to... plasmids from this depositor's lab. Plasmid Description Vector Type Selectable Marker PI Publication ...

  15. Depositor Collections

    Type
    Collection
    ...Collection CRISPR CRISPR-on: an RNA-guided transcriptional activator system - Jaenisch Locus-specific ...Rinehart FreeGenes Project Open Enzyme Collection Screening Multiplexed Overexpression of Regulatory Factors...

  16. Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids

    Type
    Collection
    ...cleaves the target DNA. The Joung lab recently described gRNA and Cas9 expression vectors and validated...Engineering newsgroup here . Links to additional pages describing Joung Lab CRISPR-Cas/RGN reagents: Cpf1 expression...

  17. Brain Initiative Collection

    Type
    Collection
    ... with Addgene materials. Plasmids ID Plasmid Description PI Viral Preps Addgene distributes ready-to-use...Learn more about our viral service ID Viral Prep Description PI 65417-AAV8 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine...labeling in neurons. Useful for nuclear isolation and scRNA-seq applications. Jonathan Ting 163909-AAV9 pAAV_hSynapsin_psychLight2...

  18. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...

  19. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...

  20. CRISPR Plasmids - Parasites

    Type
    Collection
    ...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...
Showing: 61 - 80 of 134 results