Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 112 results for: cell;

Showing: 61 - 80 of 112 results

  1. Deisseroth INTRSECT Collection

    Type
    Collection
    ...to enable three-feature cell targeting. C) Example of viral targeting of cells expressing Cre AND Flp,...parameters. Figure 1: Examples of intersectional cell population definitions using recombinases. A) Cre...adeno-associated virus (AAV)-borne payloads to be expressed in cells based on a doubly-specified combination of genetic...Neve R, Huang ZJ, Deisseroth K. 2014. Targeting cells with single vectors using multiple-feature Boolean...Cortex Reveals Local and Long-Range Circuit Motifs. Cell Rep. 28(13):3450-3461. PubMed (Link opens in a new...well-defined cellular sub-populations has generally been limited to single-component cellular definitions...multiple recombinase-expression strategies to define cellular sub-populations of interest, including dual-transgenic...

  2. Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection

    Type
    Collection
    ...Zhang Lab Plasmids The Human TFome Library Stem Cells Protocols & References MORF Plasmids MORF Pooled...Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F. Cell 2023 Jan 5; 186(1):209-229. PubMed (Link opens in...be used for screening transcription factors for cellular programming or cancer drug resistance. Depositor...transcription factors for applications such as cellular programming or cancer drug resistance. Protocols...

  3. Lentivirus Plasmids

    Type
    Collection
    ...Lentivirus can transduce both dividing and non-dividing cells without a significant immune response. These viruses... in the viral particle) is delivered to a target cell upon infection. When lentivirus is used for research...the researcher wants delivered to specific target cells. This genome is encoded by plasmids called "transfer...encoded by the transfer plasmid is toxic to mammalian cells, it can be difficult to efficiently produce viral...

  4. Worm Expression Resources

    Type
    Collection
    ...Worms are particularly well suited for studies in cell biology, development, and aging. Scientists can .... elegans hermaphrodite consists of 959 somatic cells, which makes them attractive for imaging and fluorescence...researchers to express desired constructs in precise cell types and/or developmental timeframes. Here we highlight...

  5. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...e.g., pHelper) and specific packaging cell lines (e.g., HEK293 cells) during AAV production. Read our AAV...AAV can transduce both dividing and non-dividing cells with a low immune response and low toxicity. Although...

  6. CRISPR Plasmids - Parasites

    Type
    Collection
    ...sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ), a mechanism...PAM site. When a repair template is present, the cell may repair a DSB using homology-directed repair ...

  7. AAV Viral Preps

    Type
    Collection
    ... tools that effectively transduce many mammalian cell types. AAV is used in a wide range of experiments...Tetracycline Transactivators, Affinity Purification, Cell Ablation Monosynaptic Neuronal Tracing Rabies Virus-Based...

  8. Plasmid Collections

    Type
    Collection
    ...Mammalian RNAi Microbiology Plant Expression Stem Cells Synthetic Biology Tet Inducible Expression Worm ...BioBricks Foundation BRAIN Initiative Collection Cell Migration Consortium Center for Genome Engineering...

  9. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ), a mechanism...PAM site. When a repair template is present, the cell may repair a DSB using homology-directed repair ...

  10. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...Can I directly transfect cloning grade DNA into cells? Cloning grade DNA is designed for use in cloning...cloning applications. Direct transfection into cells is not recommended. Can I order more than 2 µg of cloning...

  11. Chemogenetics Plasmids

    Type
    Collection
    ...allowing a scientist to control signaling within cells expressing these engineered receptors. Chemogenetic...engineered receptor is coupled to and the specific cell type where the receptor is expressed, these receptors...

  12. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...abundant forms of posttranslational modifications in cells and research into its many roles in protein function...interactions can be discovered by fluorescence-activated cell sorting (FACS). The mode #2 phosphosite library ... Production of Recombinant Phosphoproteins Using Cell-Free Protein Synthesis. Oza JP, Aerni HR, Pirman...Ordureau A, Paulo JA, Rinehart J, Harper JW. Molecular Cell . 2015. Sep 9. pii: S1097-2765(15)00662-0. doi: ...

  13. CRISPR Plasmids - Plants

    Type
    Collection
    ...sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ), a mechanism...PAM site. When a repair template is present, the cell may repair a DSB using homology-directed repair ...Cas protein and a target-specific gRNA in the same cell at the same time. Single plasmids containing both...

  14. Mammalian RNAi Tools

    Type
    Collection
    ... in therapeutic settings. As a tool in mammalian cell systems, silencing is achieved through the delivery...). Origins and mechanisms of miRNAs and siRNAs . Cell, 136 (4), 642–655. PubMed (Link opens in a new window...

  15. Cre-lox system

    Type
    Collection
    ...allowing for direct comparison of Cre+ and Cre- cells. While Cre-lox recombination is a very useful tool...promoter like CAG, or expressed only in a subset of cells under a more specific promoter (e.g. Rho-Cre is ...promoters. Expression of both N and CCre in the same cell type allows for recombination of LoxP flanked DNA...pNrl-Cre Cre-Myc, Expressed in rod photorecetor cells in the retina Nrl Mammalian Cepko 13962 pBS mPrx1... Okano 153207 pAM-AAV-mSncg-Cre Retinal ganglion cell-specific expression of Cre Mouse Sncg AAV Hu 154018...of loxP plasmids, reporter genes indicate which cells have undergone Cre recombination. These plasmids...37402 Cre Stoplight 2.4 Zsgreen is expressed in cells without Cre, mCherry is expressed with Cre Mammalian...

  16. Brain Initiative Collection

    Type
    Collection
    ...real-time interrogation and monitoring of brain cells and circuits during behavior. Long desired by researchers...A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. Human...A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. Cre-dependent...genetically encoded dopamine sensor RdLight1 in mammalian cells. Lin Tian 125708-AAV9 pAAV-hSynapsin1-RdLight1 Expresses...neurons Yulong Li 158757-AAV8 pAAV-CAG-SomaGCaMP6f2 Cell body-targeted GCaMP6f, under CAG promoter: GCaMP6f... Edward Boyden 158757-AAV9 pAAV-CAG-SomaGCaMP6f2 Cell body-targeted GCaMP6f, under CAG promoter: GCaMP6f...

  17. Genetic Code Expansion

    Type
    Collection
    ...expanding the genetic code in bacteria and mammalian cells....codons, and tRNA synthetases are introduced into the cell on plasmids and the new amino acid is introduced...Once these plasmids have been introduced in the cells, the non-canonical amino acid can be incorporated...match the growth medium, ncAA concentration, and the cell lines used previously. Remember that the orthogonal... – GFP for E. coli or mCherry-GFP for mammalian cells. You should also express the protein with and without...tRNA synthetase for use in E.coli and Mammalian Cells. Many of the plasmids also contain one or more copies...

  18. CRISPR References and Information

    Type
    Collection
    ...interest and correspond to edited and non-edited cell populations. For more information, see the Hsiau...138 KB Musunuru CRISPRs in human pluripotent stem cells pCas9_GFP ; gRNA empty vector Protocol at StemBook...Bauer Protocol for Genomic Deletions in Mammalian Cell Lines pSpCas9(BB) (pX330) Protocol at Addgene Parrott...

  19. Fluorescent Protein Guide: Activity Regulation

    Type
    Collection
    ...components or take advantage of existing GFP-lines for cell-specific manipulation. Plasmids Fluorescent Protein...Fluorescent Protein Resources: Empty Backbones FRET Subcellular localization Biosensors Optogenetics Background...

  20. KLF Research Plasmids

    Type
    Collection
    ...regulators with diverse roles in processes such as cell proliferation, differentiation, inflammation, metabolism...
Showing: 61 - 80 of 112 results