We narrowed to 80 results for: expression vector

...full sequencing of the final viral vector preparation. These viral vector preparations were produced with...rAAV2tYF-CB-hRS1, a Recombinant Adeno-Associated Virus Vector Expressing Retinoschisin. Hum Gene Ther Clin Dev . Sep... Viral Vector Packaging Service AAV University of Florida Serotype...Serotype Testing Panel for the Eye and Brain Viral Vector Packaging Service: University of Florida Serotype...Viviana Gradinaru PHP AAV Serotypes Blog: Viral Vectors As part of our Viral Service , Addgene is producing...of our standard viral production , these viral vectors undergo quality control, including AAV titration... in vitro as well as in vivo delivery where the vector is diluted by the physiologic body fluids, such...

...gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors page and search the table for "Xenopus". ...

... Viral Vector Packaging Service AAV Recombinases Viral Vector Packaging Service: Recombinases...recombinases, which can be used to control gene expression. See our Cre-lox Plasmids page for more information... eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know...format. Please note this does not guarantee viral vector packaging service, but lets us know what viruses...

...gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors page and search the table for "C. elegans...

...gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors page and search the table for "Drosophila...

...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors By Species Mammalian Bacteria Drosophila Plant ...CRISPR-FokI plasmids. Plasmids are available for expression in mammalian systems and Drosophila. Mammalian...

...gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors page and search the table for "Yeast". Activate...

...format into lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which Cas9 is included...tools require constitutive expression of the dCas9 fusion and/or expression of multiple fusions. The effects...sequences . Once you’ve selected your target, you’ll express your gRNA and Cas9 protein, which will form a ribonucleoprotein...the DSB site. Therefore, a population of cells expressing Cas9 and a gRNA will result in a diverse array...single plasmid ensures that all gRNAs will be expressed in the same cell. This is called multiplexing ...targeting in the double digits. These multiplex gRNA vectors can be used to knock out, activate, or repress ...multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9 Endonuclease CRISPR specificity...

...Example of viral targeting of cells expressing Cre AND Flp, here expressed through a double transgenic animal...recombinase-expressing mouse lines 2–9 and combinations of transgenic recombinase-expressing animal lines...Find intersectional expression plasmids that combine multiplexed recombinases, including Cre, Flp, and...and VCre to enable viral expression of molecular payloads in target populations defined by multiple parameters...understanding of neural circuit functionality. Precisely expressing these tools in well-defined cellular sub-populations... a single gene or projection). Intersectional expression approaches combine multiplexed recombinases, ... including Cre, Flp, and VCre to enable viral expression of molecular payloads in target populations defined...

...gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors page and search the table for "Bacteria"....

...to permit construction of RAS pathway gene expression vectors for in vitro and in vivo research. The RAS...plasma membrane. Cancer Impact Ras is ubiquitously expressed in all cells and all tissues. Ras can activate...Resources RAS Pathway Clone Collection 2.0: Set of vectors from the Esposito Lab for use with the Gateway®...

... by expressing and purifying the phosphosite library as GST-fusion peptides (“Mode #1” expression). The...can also be introduced into a second expression vector encoding a split mCherry fluorescent reporter, ...characteristics, and an overall improved ease of use when expressing recombinant phosphoproteins. These attributes... origins. Therefore, when choosing plasmids to express the phosphoprotein of interest, a medium copy RSF1030...2023) and find general tips on phosphoprotein expression in rEcoli XpS in the Methods for iSPI, rEcoli...phosphorylation-dependent protein-peptide interactions (“Mode #2” expression, Barber et al., 2018). The phosphosite library...Mode #2 phosphosite library configuration for overexpression in E. coli . The phosphosite DNA library can...

...Douglas Kim , GENIE Project Calcium Soma-targeted expression of jGCaMP8f, GCaMP7f, or GCaMP6f Precision Calcium...6. Edward Boyden Calcium Intersectional viral expression of GCaMP6m/s in cells depdent on Cre, Flp, or... Dual- and Triple-Feature Intersectional Single-Vector Delivery of Diverse Functional Payloads to Cells...Karl Deisseroth , INTRSECT 2.0 Project Calcium Expression of GCaMP6f in PV cortical interneurons under ... Oct 25;9(1):4440. Eric Schreiter Calcium AAV expression of photoconvertible fluorescent protein-based..., Eric Schreiter Calcium Intersectional viral expression of sRGECO (constitutive or Cre- or Flp-dependent...

... Viral Vector Packaging Service AAV Controls Viral Vector Packaging Service: Control...Control AAV Browse our control AAV that express fluorescent proteins under general promoters. These AAV can... eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know...format. Please note this does not guarantee viral vector packaging service, but lets us know what viruses...

...3.1 pmTurquoise2-N1 , pCS2+mNeonGreen-C Cloning Vector , mNeonGreen-mTurquoise2 CyPet YPet 435 0.51 530... 128,000 0.45 6.4 4.7 pCS2+mNeonGreen-C Cloning Vector , pcDNA3-mRuby2 , mNeonGreen-mRuby2-FRET-10 SYFP2...fluorophores through linkers, fluorescent protein expression level and maturation time, and other environmental...

...protein translation machinery. A synthetase plasmid expressing the engineered tRNA and its cognate aaRS enzyme...make sure that these conditions are true. Always express first with a control reporter gene: GFP for E. ...mCherry-GFP for mammalian cells. You should also express the protein with and without the ncAA in the media... tRNA gene. ID Plasmid Synthetase Origin ncAA Expression Codon PI 31186 pEVOL-pAzF p-azidohenylalanine...amino acid incorporation. ID Plasmid Gene/Insert Vector Type PI Return to top Bacterial Strains Browse ...mutated, serB deleted for phosphoserine GCE. For expressing phosphoserine-containing proteins using genetic...197656 BL21(DE3) ΔserC serC gene knocked out; for expressing proteins containing site-specific non-hydrolyzable...

... Viral Vector Packaging Service AAV Biosensors Viral Vector Packaging Service: Biosensor...Biosensor AAV Browse our AAV that express fluorescent biosensor proteins, including calcium sensors, under... eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know...format. Please note this does not guarantee viral vector packaging service, but lets us know what viruses...

...termed enhancers — to drive cell type-specific expression. The Allen Institute for Brain Sciences has screened... AAV delivery. The collection includes the top vectors with the highest specificity and brightness, assessed...Functional enhancer elements drive subclass-selective expression from mouse to primate neocortex. Cell Reports...

... SLCs in Gateway-compatible pENTR vectors (pDONR221). These vectors allow a fast and efficient sub-cloning...Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert Industry PI Pooled Libraries CRISPR and overexpression pooled .... Human SLC Overexpression Library 213694 Superti-Furga Inducible barcoded overexpression library of human...

...® Entry vectors from the labs of William Hahn and David Root . Multiple Lentiviral Expression (MuLE) System...finds and attacks pathogens or infected cells expressing that antigen. Antibodies produced by B cells ...factor (TNF) family of proteins are predominantly expressed by immune cells and help to regulate the immune...regulatory factor X, 5 (influences HLA class II expression) - RFXANK regulatory factor X-associated ankyrin-containing...systems. The MXS-chaining kit includes chaining vectors with multiple cloning sites and standardized building...selection cassettes and tools for inducible gene expression. Myristoylated Kinase Kit : Consists of 190 human...