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We narrowed to 94 results for: expression vector

Showing: 61 - 80 of 94 results

  1. CRISPR References and Information

    Type
    Collection
    ...cloning vector Retroviral vectors: neomycin (pSIR-neo) , GFP (pSIR-GFP) , DsRed (pSIR-DsRed-Express2) , human... sgRNA dual expression: pAC2 , pAC152 , pAC153 , pAC154 ; pmax dCas9 activator expression: pAC91 , pAC92... pooled library amplification 1 vector system: lentiCRISPR v2 2 vector system: lentiCas9-Blast and lentiGuide-Puro...Addgene Church gRNA design and cloning gRNA cloning vector PDF, 118 KB Church gRNA design and cloning for ...human pluripotent stem cells pCas9_GFP ; gRNA empty vector Protocol at StemBook O'Connor-Giles Fly: gRNA and...at Addgene Parrott NEBuilder Assembly of CRISPR vectors using ssDNA oligos p201G Cas9 ; p201B Cas9 ; p201H...design and cloning All-in-one plasmid containing expression cassette for NmCas9 and BsmBI site for cloning...

  2. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ...a site within that gene. To construct ZFN expression vectors, DNA encoding zinc finger arrays should be...sequence between the half-sites (see Nuclease Expression Vectors for more details). IMPORTANT NOTE: All zinc...plasmids also available through Addgene--the specific vector to use depends on the length of the spacer sequence...

  3. Antibody Production

    Type
    Collection
    ...recombinant antibodies (rAb) encoded by mammalian expression vectors, human embryonic kidney cells are transfected...encoding the heavy and light chains. Antibodies are expressed for 7 days and then purified from the supernatant...retained. If the target antigen is not endogenously-expressed (e.g., using an anti-GFP antibody), the target...target antigen is first transiently expressed in mammalian cells. To learn which specific QC experiments were...tissue labeling pattern is compared to the mRNA expression pattern cited in the Tissue Atlas (Link opens...species-specific HRP-conjugated secondary antibody. Protein expression is compared to an untransfected control and the...

  4. Cancer Research Plasmids and Resources

    Type
    Collection
    ...to permit construction of RAS pathway gene expression vectors for in vitro and in vivo research. TP53 Mutagenesis...Broad Target Accelerator Plasmids: Collection of vectors from the Boehm , Hahn , Meyerson , and Root Labs...pan-cancer” analyses. Cancer Pathways ORFs Kit : Set of vectors from the Sabatini & Wood Labs containing cDNAs ... Lab RAS Pathway Clone Collection 2.0 : Set of vectors from the Esposito Lab for use with the Gateway®...signaling studies, including mutant, knockdown, and overexpression constructs. Each collection page listed below...

  5. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...the 20-mer is not G. sgRNA expression from the U6 promoter of the pX330 vector is enhanced by the inclusion...including additional nucleotides for cloning and expression purposes. NOTE: Here we discuss the usage of ...42230). This plasmid allows for the simultaneous expression of sgRNA and SpCas9, but does not contain markers...or constructs in which multiple sgRNAs may be expressed from a single plasmid. Add “CACC” before the 20...s reverse complement for cloning into the pX330 vector using BbsI restriction enzyme ( Table 3 ). Add ...Prepare a 50 μl reaction mix: 100 ng circular pX330 vector, 1.0 μl annealed oligos (1 μM; see step 3.1.4 )...Cas9 construct (10 μg total). Add 0.5 μg of GFP expression construct. Electroporate cells with 250 volts...

  6. Neurodegeneration Research Collection

    Type
    Collection
    ...inducible vector . Harley et al. Cell Rep. 2023 Dec 26. Induce CNS cells with concomitant expression of aggregation-prone...Neurodegeneration Plasmid Collection A collection of plasmids expressing wild type or mutant ORFs, gene fragments, and...to neurodegenerative disorders. Find plasmids expressing HTT, C9ORF72, LRRK2, Synuclein, and more. New... dopamine research. CBA-driven, Cre-dependent expression of diphtheria toxin receptor (DTR)–GFP fusion...Dec 2. Use PiggyBac plasmids with tet-inducible expression of transcription factors for sensory, motor, ...with rapid delivery. Includes antibodies, viral vectors, animal models, purified protein, and more. Plasmids...

  7. Distribution to Industry

    Type
    Collection
    ...while also adding additional filters such as expression, vector type, species, and more. Browse Browse the...for Golden Gate assembly. MoClo Toolkit Plant Expression Sylvestre Marillonnet 95 plasmids for cloning...Libraries CRISPR Thorsten Stiewe Use these donor vector libraries for CRISPR/Cas9-mediated saturation genome...

  8. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...fusing a biotin acceptor site to dCas9 and co-expressing BirA biotin ligase, as seen in the CAPTURE system...purify genomic loci. Plasmids are available for expression in mammalian systems, bacteria, and yeast. Mammalian...

  9. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...codon-optimized for expression in bacteria and cloned into an IPTG-inducible His-tag expression vector. All His-tags...sustainable MDH-related CURE focusing on protein expression, structure and function, kinetics and mechanisms...

  10. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...Bydder SF, Zhou Y, Nielsen LK. Dual gene expression cassette vectors with antibiotic selection markers for...Botman et al., 2019 . FP Tagging Yeast tagging vectors to create fusions of proteins of interest with ...yeast codon-optimized fluorescent proteins. These vectors are based on the pFA6a-link plasmid (Sheff et al...FPs in yeast. Vectors are based on the pDRF1 plasmid (Loqué et al., 2007) containing the PMA1 promoter and...blue, green, and red fluorescent protein tagging vectors for S. cerevisiae. PLoS One. 2013 Jul 2;8(7):e67902...Marker Tags Publication FP Overexpression Plasmids for constitutive overexpression of codon-optimized FPs ...Find plasmids to overexpress various fluorescent markers and tag your proteins of interest with fluorescent...

  11. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...getting all components of the Cascade complex to express equally. To combat this, some CRISPR-Cas3 plasmids...Cascade-Cas3 plasmids. Plasmids are available for expression in mammalian systems, bacteria, and plants. Mammalian...

  12. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...gRNA expression vectors available from Addgene....-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...experimental use of these plasmids. ID Plasmid Gene/Insert Vector Type PI Publication Do you have suggestions for...

  14. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Douglas Kim , GENIE Project Calcium Bicistronic vector expressing GCaMP6 and mRuby2 for imaging neural activity... Huebener , Tobias Rose Calcium Soma-targeted expression of jGCaMP8f, GCaMP7f, or GCaMP6f Precision Calcium...6. Edward Boyden Calcium Intersectional viral expression of GCaMP6m/s in cells depdent on Cre, Flp, or... Dual- and Triple-Feature Intersectional Single-Vector Delivery of Diverse Functional Payloads to Cells...Karl Deisseroth , INTRSECT 2.0 Project Calcium Expression of GCaMP6f in PV cortical interneurons under ... Aug 4;11(1):3881. Adam Cohen Calcium GCaMP6f expression in forebrain GABA-ergic interneurons under the... Oct 25;9(1):4440. Eric Schreiter Calcium AAV expression of photoconvertible fluorescent protein-based...

  15. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...Further advancements have been made by optimizing expression of the fusions, modifying the linker region between... editing plasmids. Plasmids are available for expression in mammalian systems, bacteria, plants, yeast...

  16. Recombinases AAV Preps

    Type
    Collection
    ... Viral Vector Packaging Service AAV Recombinases Viral Vector Packaging Service: Recombinases...recombinases, which can be used to control gene expression. See our Cre-lox Plasmids page for more information... eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know...format. Please note this does not guarantee viral vector packaging service, but lets us know what viruses...

  17. Deisseroth INTRSECT Collection

    Type
    Collection
    ...Example of viral targeting of cells expressing Cre AND Flp, here expressed through a double transgenic animal...recombinase-expressing mouse lines 2–9 and combinations of transgenic recombinase-expressing animal lines...Find intersectional expression plasmids that combine multiplexed recombinases, including Cre, Flp, and...and VCre to enable viral expression of molecular payloads in target populations defined by multiple parameters...understanding of neural circuit functionality. Precisely expressing these tools in well-defined cellular sub-populations... a single gene or projection). Intersectional expression approaches combine multiplexed recombinases, ... including Cre, Flp, and VCre to enable viral expression of molecular payloads in target populations defined...

  18. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ... by expressing and purifying the phosphosite library as GST-fusion peptides (“mode #1” expression). The...library can also be introduced into a second expression vector encoding a split mCherry fluorescent reporter...characteristics, and an overall improved ease of use when expressing recombinant phosphoproteins. These attributes... origins. Therefore, when choosing plasmids to express the phosphoprotein of interest, a medium copy RSF1030... 2023 and find general tips on phosphoprotein expression in rEcoli XpS in the Methods section link below...phosphorylation-dependent protein-peptide interactions (“mode #2” expression, Barber et al, Nat. Biotech. 2018). The phosphosite...

  19. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...CRISPR-FokI plasmids. Plasmids are available for expression in mammalian systems and Drosophila. Mammalian...
Showing: 61 - 80 of 94 results