... HDR occurs at a much lower efficiency than NHEJ. ID Plasmid Gene/Insert Selectable Marker PI Publication...expression plasmids that have been designed for use in parasites. CRISPR... CRISPR Parasites CRISPR Plasmids: Parasites...Parasites Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional ...development. This collection brings together some of the plasmids that have been used in these efforts. The content...protein-coding genes. Do you have suggestions for other plasmids that should be added to this list? Fill out our...

...backbones/controls): ID Plasmid GenBank Key Features 26092 p15TV-L EF456736 Hexahistidine tag with TEV cleavage...Consortium Plasmids Structural Genomics Consortium Plasmids You may also like... David Waugh Lab plasmids The...collection by All Plasmids SGC Toronto SGC Oxford SGC Stockholm Bacterial Expression Plasmids (empty backbones...Request Plasmids To order, Login or Register a new account. Add the kit or individual plasmids to your...Plasmids from the Structural Genomics Consortium (SCG), which are primarily used for determining protein...the public domain without restriction. Many SGC plasmids from the Toronto, Oxford and Stockholm sites have...

... Antibody Suggestion Form . Browse All Antibodies ID Recombinant Antibody Description Source Species Isotype...develop and refine these application lists. The plasmids we use to produce antibodies are available as ...? Check out our current collection of Antibody Plasmids , fill out our Suggest an Antibody form, or email...

...information on the experimental use of these plasmids. ID Plasmid Gene/Insert Vector Type PI Publication... CRISPR gRNAs CRISPR Plasmids: gRNAs Browse CRISPR Plasmids By Function Genome Editing Cut ...gRNA Plasmids Please note that the following table lists both published and unpublished plasmids containing...Tools CRISPR Blog Posts The table below lists gRNA plasmids designed to target various genes or genomic regions...target sequence before using any of these gRNA plasmids and review the publication associated with each...here to start the deposit process and have your plasmids added to the list. You can also email [email protected] Do you have suggestions for other plasmids that should be added to this list? Fill out our Suggest...

...by next generation sequencing Plasmids Available as Cloning Grade DNA ID Plasmid Description Industry ...Grade DNA (cgDNA) for over 200 plasmids in our collection. The plasmids available in this format include...variety of popular plasmids and backbones with high cloning potential. By making these plasmids available as... our most popular plasmids! DNA... DNA Service: Cloning Grade DNA Service Details Plasmids Available FAQ Addgene...and extraction steps required when one receives plasmids in bacterial stabs. Cloning Grade DNA is an Addgene...this time, Addgene has made a limited number of plasmids available as cloning grade DNA that we hope to...

...or REAL-Fast assembly process or lists of plasmid ID numbers required for FLASH assembly. Links to additional... like... Keith Joung Lab plasmids Daniel Voytas Lab plasmids CRISPR plasmids The Joung Lab has developed...Gene Additional Plasmids compatible with the REAL Assembly TALEN Kit: TALE-LSD1 plasmids for epigenome ... simplify the construction of TALEN expression plasmids using REAL, REAL-Fast or FLASH assembly. This ...Assembly TALEN Kit Individual REAL TALE Repeat Plasmids TALEN Expression Vectors for REAL, REAL-Fast and...

...transferred to Addgene for distribution. Look up the IDs of particular Penn Vector Core catalog items. Virus-associated...Overview of Addgene's viral preparation service for plasmids and pooled libraries. Viral preparation options...produces high-quality viral vector preps from select plasmids in our collection. We know that viral vector production... production is a common step after requesting plasmids from Addgene, and by providing this service, we...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....

...allow you to insert and induce your gene of interest. ID Plasmid Description Co-expressed tTA, rtTA, or TetR...expresses the transactivator for your tetraycline system. ID Plasmid Description Element On or off PI 26429 pLenti...Expression Additional Resources: Browse Tetracycline Plasmids Tet-regulated Lentiviral RNAi Vectors Curated ...Tetracycline Off Tetracycline On Experimental Tips Tet Plasmids Background To advance the study of gene function...uptake. Search our collection for all tetracycline plasmids. Tet System and the Tet Response Element (TRE)...detailed information about cloning and additional plasmids tetR-KRAB On Aebischer & Trono 16542 pBI-MCS-EGFP... expression; see article for additional tookit plasmids third-generation rtTA On Elledge 19407 pTREtight2...

...otherwise noted, these plasmids are meant for expression in mammalian systems. ID Plasmid Luciferase Type...luciferase expression plasmids; these are noted with the viral service icon . ID Plasmid Luciferase Type...interest. ID Plasmid Description Gene/Insert PI Return to Top Do you have suggestions for other plasmids that...luciferase. Plasmids are Gateway cloning compatible. Learn more about DULIP in our 27 Hot Plasmids from 2016...luciferase reporter. These plasmids improve over the original STARR-seq plasmids by using the bacterial ORI...Browse Addgene's collection of luciferase plasmids for assays of transcriptional regulation and bioluminescent... Plasmid Collections Luciferase Plasmids Luciferase Plasmid Collection You may also like...

... such as pX458 (Addgene plasmid ID 48138) or pX459 (Addgene plasmid ID 48139), which include GFP and puromycin... the pSpCas9(BB) plasmid (pX330) (Addgene plasmid ID 42230). This plasmid allows for the simultaneous ...Mammalian Cell Lines Other Addgene Resources CRISPR Plasmids CRISPR Guide Addgene Blog Posts Addgene is proud... protocol involves the delivery of CRISPR/Cas9 plasmids by electroporation 4 . This protocol is described...However, transient transfection of CRISPR/Cas9 plasmids may be successfully adapted to numerous cell ...

...Cerebellum Whole brain Addgene ID Plasmid Allen Institute ID Enhancer ID FP/Recombinase Labeled Population...Systemic Capsids AAV Packaged on Request AAV Guide Plasmids Publications Enhancer AAV plasmids are plasmids...Find AAV enhancer plasmids for fluorescent labeling and recombination across brain regions and populations...) . Allen Institute for Brain Science Enhancer Plasmids The Allen Institute for Brain Science Enhancer...Enhancer Collection contains best-in-class AAV plasmids to target a variety of cell populations in different...tested in mouse brains. This collection contains plasmids for cell populations in the following regions:...

...ready-to-use AAV preparations of many chemogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type ...Chemogenetics Plasmids Browse, sort, or search this table to find chemogenetics plasmids in our collection... Plasmid Collections Chemogenetics Plasmids Chemogenetics Plasmid Collection You may also ...: Chemogenetics vs. Optogenetics Chemogenetics plasmids encode genetically engineered receptors that are...Promoter Tags PI Do you have suggestions for other plasmids that should be added to this list? Fill out our...

...window) Article Individual plasmids can be ordered via the links below: ID Plasmid 46294 pBS-Hsp70-Cas9... CRISPR flyCRISPR Lab CRISPR Plasmids flyCRISPR Plasmids Available from Addgene You may also like...Browse CRISPR Cas9 gRNA plasmids for drosophila research from the O'Connor-Giles, Wildonger, and Harrison... repaired DSBs. Coinjection of phsp70-Cas9 and plasmids encoding two chiRNAs can induce defined deletions...model and non-model invertebrate species. These plasmids are described in: Genome engineering of Drosophila...

...Addgene #110821 ) VPR ( Addgene #63798) Plasmids from Collection ID Plasmid Description PI Publications Kaufman...constructs. Plasmids 171098 and 175570 allow conversion to a dual guide construct. Plasmids 175573 and ...Find CRISPR plasmids to study the regulatory networks involved in retinal development. Depositor...-guide cassettes to make dual guide expressing plasmids dCas9-KRAB-MeCP2 fusion for gene and non-coding... Figure 1: Schematic of the CRISPR Collection. Plasmids 159654 and 159655 are single guide expressing ...175574 express dCas9-KRAB-MeCP2 for repression. Plasmids 175571 and 175572 express dCas9-VPR for activation...oligonucleotides with the appropriate overhangs: The plasmids listed here are provided as empty backbones that...

...items will be shipped as stabs at room temperature. ID Name 13421 pAC-Kan-alphaGal4 13422 pBAC-lacZ 13424...like... Keith Joung Lab plasmids Daniel Voytas Lab plasmids Scot Wolfe Lab plasmids Detailed Information ...Plamids for Oligomerized Pool ENgineering (OPEN) method has been used to engineer ZFNs that function ...Depositing Labs: Keith Joung This kit consists of plasmids and strains used to engineer ZFNs via the Oligomerized.... This kit consists of 5 individually packaged plasmids and 3 individually packaged strains, which are...bacterial stabs at room temperature. Individual plasmids can be ordered from each plasmid page and will...Nature Protocols paper . How to Cite this Kit These plasmids were created by your colleagues. Please acknowledge...

...protein (MERS, CoV-1, CoV-2), modification, or variant. ID Plasmid Description Mutations Industry PI Due to ...Accessory Plasmids Envelope and Packaging Plasmids Popular Lentiviral Envelope and Packaging Plasmids Popular...Envelope and Packaging Plasmids Reporter Plasmids A few examples of reporter plasmids that can be used for...particles See Addgene's Viral Plasmids and Resources page for more information. Plasmids Expressing Spike and ...Addgene's collection of plasmids for viral pseudotyping with SARS-CoV-2 spike protein. Collections...optimization, deletion of the C-terminal 18-21 amino acids and a D614G change which is present in many circulating...circulating variants. The table below lists expression plasmids that are suitable for generating pseudotyped virus...

...find plasmids containing other RNAi components, like argonaute, please search our full site . ID Plasmid...BioRender.com. shRNA Plasmids Browse our selection of empty vectors for cloning in shRNAs. Most plasmids are in a ...Plasmids for mammalian RNA interference (RNAi) experiments at Addgene; browse by constitutive lentiviral...Mammalian RNAi Mammalian shRNA Tools for RNAi Browse Plasmids Resources RNA interference (RNAi) is an RNA-mediated...with some retroviral and AAV backbone options. Plasmids for constitutive expression as well as those that...Other Addgene viral protocols Lentiviral packaging plasmids Web Resources Trono Lab Lentivectors Toolbox Optimized...

...site", "active site loop", or "subunit interface") ID Plasmid Description Gene/Insert Mutations Additional...collaborations between institutions. With these plasmids, faculty can begin a sustainable MDH-related CURE...and metabolic pathways and protein interactions. Plasmids A diverse set of wild-type MDH genes from several...page. Use the search bar or sort buttons to find plasmids based on: Organism or species (e.g., human, watermelon...

...virus. AAV Vectors for Monosynaptic Neuronal Tracing ID Name Description Serotype PI 52473 pAAV-synP-FLEX-splitTVA-EGFP-B19G...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....

...anti-rabbit") ID Plasmid Description Gene/Insert PI Return to top Do you have suggestions for other plasmids that...Apr 8. Markus Seeger Antibody Plasmids The table below highlights plasmids that feature expression of antibodies... ready-to-use recombinant antibodies made from plasmids in our collection! Many of these monoclonal antibodies...More Cloning and Expressing Antibodies Antibody Plasmids Antibodies are large proteins made by the immune...antibodies, created by cloning antibody components into plasmids and expressing these in bacteria, mammalian cells...Trimmer Lab Recombinant mAb Collection . Once the plasmids are transiently transfected into mammalian tissue...nanobodies). For example, scientists often use plasmids encoding scFv fragments that bind to the GCN4 ...