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Showing: 61 - 80 of 120 results

  1. Zinc Finger Consortium: Nuclease Expression Vectors

    Type
    Collection
    ...cell types. Zinc finger arrays created using ZFC reagents for either OPEN or modular assembly can be easily... cells or zebrafish) pDW1775 (for expression in plants) Vectors encoding heterodimeric FokI nuclease domains...

  3. All Antibodies

    Type
    Collection
    ... , which also includes other types of affinity reagents such as scFvs, DARPins, Fabs, and secondary antibodies...addgene.org to help us improve our resources! Acknowledgments This project is supported by a NIH BRAIN Initiative...

  5. Synthetic Biology - Overview

    Type
    Collection
    ...plasmids related to metabolic pathways and auxiliary components. Networks and Gene Regulation Browse plasmids...both naturally occurring and synthetic regulatory elements, including promoters, terminators, repressors,...

  6. Retrovirus Plasmids

    Type
    Collection
    ...γ-retroviruses. γ-Retroviruses can package relatively high amounts of DNA (up to ∼8 kb), and infection results in ...immortalized cells (see Weinberg Lab page for resistance variants) Weinberg 1773 pBABE-hygro-hTERT MoMLV Expression...

  7. Antibody Production

    Type
    Collection
    ...’s recommended applications. The specific QC experiments performed varies for each antibody catalog item... mammalian cells. To learn which specific QC experiments were performed on your lot, please email help...

  8. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...Promoter PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand breaks in...strategies reduce unwanted off-target effects. Nickase mutants can also be used with a repair template to introduce...

  9. CRISPR Plasmids - Yeast

    Type
    Collection
    ... Marker PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand breaks in...strategies reduce unwanted off-target effects. Nickase mutants can also be used with a repair template to introduce...

  10. mTOR Pathway

    Type
    Collection
    ...lysosome biogenesis. mTORC2 does not respond to nutrients, but it is activated by growth factors, including... to find available plasmids for the gene. For components with multiple isoforms or subunits, individual...

  11. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...transcriptase. Engineering of the PE enzyme and related components have helped to improve the efficiency of editing... for expression in mammalian systems, bacteria, plants, and Drosophila. Mammalian ID Plasmid Gene/Insert...

  12. Worm Expression Resources

    Type
    Collection
    ...attractive for imaging and fluorescence microscopy experiments. The C. elegans genome is well studied and hundreds...plasmids that facilitate detection of genome-editing events. Developed in Craig Mello's lab and described in...

  13. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...Promoter PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand breaks in...strategies reduce unwanted off-target effects. Nickase mutants can also be used with a repair template to introduce...

  14. TALEN Guide

    Type
    Collection
    ... Similar in principle to the ZF nuclease, the components of the array recognize individual nucleotides...Gate cloning method to assemble “multiple DNA fragments in an ordered fashion in a single reaction.” Using...

  16. Plasmids for Stem Cell Research

    Type
    Collection
    ...create cell lines with endogenously-tagged gene variants or browse Addgene’s entire collection of Stem ...Disease-Specific Phenotypes of Motor Neurons from Human ALS Patients. Cell Rep. 2016 Jan 5;14(1):115-28. Zhang Adult...

  17. Neuroscience

    Type
    Collection
    ...addgene.org to help us improve our resources! Acknowledgments This project is supported by a NIH BRAIN Initiative...

  18. Synthetic Biology - Metabolism

    Type
    Collection
    ...biology plasmids related to metabolic pathways and components. Examples Include: Biofuels Natural Product Pathways...
Showing: 61 - 80 of 120 results