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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 123 results for: ELL

Showing: 81 - 100 of 123 results

  1. TALEN Engineering

    Type
    Collection
    ...Tesson et al., Nat Biotechnol. 2011 ) Human somatic cells ( Miller et al., Nat Biotechnol. 2011 ; Reyon and...., Nat Biotechnol 2012 ) Human pluripotent stem cells ( Hockemeyer et al., Nat Biotechnol. 2011 ) TALE...TALE transcriptional activators in human cells ( Maeder et al., Nat Methods 2013 ) More detailed information...

  2. Mammalian RNAi Tools

    Type
    Collection
    ... in therapeutic settings. As a tool in mammalian cell systems, silencing is achieved through the delivery...options. Plasmids for constitutive expression as well as those that allow for conditional (Cre-lox) or...). Origins and mechanisms of miRNAs and siRNAs . Cell, 136 (4), 642–655. PubMed (Link opens in a new window...

  3. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ), a mechanism...PAM site. When a repair template is present, the cell may repair a DSB using homology-directed repair ...Cas protein and a target-specific gRNA in the same cell at the same time. Single plasmids containing both...

  4. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ), a mechanism...PAM site. When a repair template is present, the cell may repair a DSB using homology-directed repair ...Cas protein and a target-specific gRNA in the same cell at the same time. Single plasmids containing both...

  5. CRISPR Plasmids - Yeast

    Type
    Collection
    ...sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ), a mechanism...PAM site. When a repair template is present, the cell may repair a DSB using homology-directed repair ...Cas protein and a target-specific gRNA in the same cell at the same time. Single plasmids containing both...

  6. TALEN Guide

    Type
    Collection
    ...a typo in the latest draft of your dissertation. Well, that will probably never happen. Yet with transcription...summer of 2011 and has already become the best-selling kit in Addgene’s history. Dr. Keith Joung’s lab.... Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Sander JD, Cade L, Khayter...

  7. CRISPR Plasmids - Plants

    Type
    Collection
    ...sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ), a mechanism...PAM site. When a repair template is present, the cell may repair a DSB using homology-directed repair ...Cas protein and a target-specific gRNA in the same cell at the same time. Single plasmids containing both...

  8. Tags and Other Markers

    Type
    Collection
    ...catalog that target epitope tags and other common cellular proteins. These ready-to-use recombinant monoclonal...application-specific validation and quality control by Addgene as well as by our trusted partner labs. To learn more about...

  10. Fluorescent Protein Guide: Activity Regulation

    Type
    Collection
    ...Fluorescent Protein Resources: Empty Backbones FRET Subcellular localization Biosensors Optogenetics Background...components or take advantage of existing GFP-lines for cell-specific manipulation. Plasmids Fluorescent Protein...

  11. KLF Research Plasmids

    Type
    Collection
    ...Depositing Labs Juan Belmonte Linzhao Cheng Jose Cibelli George Daley Jin-Tang Dong Steven Dowdy Scott Friedman...regulators with diverse roles in processes such as cell proliferation, differentiation, inflammation, metabolism...

  12. MAPK Plasmids

    Type
    Collection
    ...Mitogen-activated protein kinases (MAPKs), also known as extracellular signal-regulated kinases (ERKs), are serine/...threonine-specific protein kinases. They regulate diverse cellular programs by acting as an integration point for...

  13. Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids

    Type
    Collection
    ...plasmids (AsCpf1 and LbCpf1) for use in mammalian cells SpCas9 variants with no detectable off-target effects...CRISPR-Cas/RGN expression plasmids for use in human cells CRISPR-Cas/RGN expression plasmids for use in zebrafish...

  14. AAV Viral Preps

    Type
    Collection
    ... tools that effectively transduce many mammalian cell types. AAV is used in a wide range of experiments...Tetracycline Transactivators, Affinity Purification, Cell Ablation Monosynaptic Neuronal Tracing Rabies Virus-Based...

  15. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ), a mechanism...PAM site. When a repair template is present, the cell may repair a DSB using homology-directed repair ...

  16. Fluorescent Protein Guide: In Vivo Imaging

    Type
    Collection
    ...Fluorescent Protein Resources: Empty Backbones FRET Subcellular localization Optogenetics Background In vivo ...stable imaging of protein interactions in living cells and live mice. Proteins for in vivo imaging have...

  17. Plasmid Collections

    Type
    Collection
    ...Mammalian RNAi Microbiology Plant Expression Stem Cells Synthetic Biology Tet Inducible Expression Worm ...BioBricks Foundation BRAIN Initiative Collection Cell Migration Consortium Center for Genome Engineering...

  18. Chemogenetics Plasmids

    Type
    Collection
    ...allowing a scientist to control signaling within cells expressing these engineered receptors. Chemogenetic...engineered receptor is coupled to and the specific cell type where the receptor is expressed, these receptors...

  19. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...Can I directly transfect cloning grade DNA into cells? Cloning grade DNA is designed for use in cloning...cloning applications. Direct transfection into cells is not recommended. Can I order more than 2 µg of cloning...

  20. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...then be used to infect permissive cells that express ACE2, the cellular receptor for SARS-CoV-2, in a single...
Showing: 81 - 100 of 123 results