...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...require the use of lentivirus to deliver the gRNA/Cas9 to target cells. Therefore, you must be equipped...Doench and Root 2nd 2 40,710 Inzolia Human CRISPR/Cas12a Multiplex Knockout 209551 209552 Knockout Human... and Root 3rd 3–6 67,366 (Set A) 67,194 (Set B) Cas13a/C2c2 Protospacer flanking site (PFS) Library 79153...86550 Inhibition Human Weissman 3rd 10 Varies CRISPR/Cas9-assisted Removal of Mitochondrial DNA (CARM) Library... Sabatini and Lander 3rd 50 6,661 Garnett Lab MinLibCas9 Library 164896 Knockout Human Garnett 3rd 2 37,722...Human Stiewe N/A Varies 1,910–3,034 per exon Human Cas13 lncRNA Library 227014 RNA Knockdown Human Sanjana...

... protein dCas9 is expressed along a CRISPR array that contains 20 bp "addresses", where dCas9 is going...going to bind on the chromosome. When dCas9 binds to a gene, it blocks the progression of the RNA polymerase...for quick integration of the aTc-inducible pTet-dCas9 in the attachment site of the phage 186. Detailed... Bikard, D., & Van Teeffelen, S. (2018). Tuning dCas9’s ability to block transcription enables robust,...Schematic of the strain expressing two reporters and dCas9 under a P-tet promoter integrated in the chromosome... of the two reporters, mCherry and sfgfp in this case, can be controlled using a plasmid‐borne CRISPR ...

...single species or PAM binding variant of Cas9 (e.g. Wild-type SpCas9 must be used with targets that are upstream...activity. Know your target. Which species or variant of Cas9 ( S. pyogenes, S. aureus etc.) was this gRNA sequence...and Resources page have been used to indicate the Cas9 application the gRNA was designed to accomplish....Target Species Target Sequence Plasmid ID Application Cas9 Species PubMed ID Depositor OCT4 H. sapiens CTCCCATGCATTCAAACTG...10 C. elegans CGCTACCATAGGCACCACG 71516 cut VRER Cas9 mutant 26680661 Fire dpy-10 C.elegans GCTACCATAGGCACCACGAG...-10 C. elegans TCCGCTACCATAGGCACCA 71479 cut VQR Cas9 variant 26680661 Fire dpy‐10 C. elegans GCTACCATAGGCACCACGAG...Christiaen EGFP A. victoria multiple, see article 60071 dCas9-FokI S. pyogenes 24770325 Joung EGFP A. victoria...

...familial ALS cases having at least one other affected family member. These familial cases, usually inherited...range of cellular processes. The vast majority of cases (90-95%) of ALS are sporadic, having no prior family...cause) nature of the disease, a small portion of cases can be attributed to known genetic factors such ...early-onset form accounts for less than 10 percent of all cases of Alzheimer's disease. Of these early-onset forms...Target neural oxytocin receptors using an AAV-CRISPR/Cas9 strategy for gene editing across divergent rodent...species. Boender et al. Sci Adv. 2023 Jun 2. Use Cas9 in astrocytes. Endo et al Science. 2022 Nov 4. Base...

...domain death agonist CASP3 Caspase 3, apoptosis-related cysteine peptidase CASP8 Caspase 8, apoptosis-related...apoptosis-related cysteine peptidase CASP9 Caspase 9, apoptosis-related cysteine peptidase Cyclin B CCNB1 CCNB2 CCNB3...

...Technique PI Purpose pCas9 42876 CRISPR Luciano Marraffini Bacterial expression of Cas9 nuclease gRNA. For...list of our bacterial CRISPR plasmids, see here . pwtCas9-bacteria 44250 CRISPR Stanley Qi Anhydrotetracycline...Anhydrotetracycline inducible expression of wild-type Cas9 from S. pyogenes for inducing double stranded breaks. For...protein expression in stationary phase. In these cases you may want to control protein levels or to turn...pPro24-gfp contains GFP but all others are empty. pdCas9-bacteria 44249 pTetO Anhydrotetracycline Stanley...Anhydrotetracycline inducible expression of catalytically inactive Cas9 ( S. pyogenes ) which can be combined with expression...

...interest should be active, and only turned off occasionally, using tetracycline off or tTA is more appropriate...should be mostly inactive, and only turned on occasionally, using tetracycline on or rtTA is more appropriate...inducible expression for Gateway cloning; selection cassette in format: PGK-rtTA-2A-puro; see article for tagged...inducible expression of a miR-shRNA; selection cassette in format: rtTA3+IRES+Neo; see article for additional...conditional cell line generation; contains expression cassette for TetR-P2A-GFP; see article for additional constructs... rtTA On Alexeyev 14901 pCL-CTIG The inducible cassette contains tTA at its 5' end and an inducible promoter...

... FP Collection NLS Nuclear localization dCas9 plasmid - dCas9 expression plasmid without effector fusions...create stable cell lines in which the expression cassette of interest is incorporated into the host...Tet-pLKO-neo - Tet-inducible lentiviral shRNA expression pnCasPA-BEC - A cytidine deaminase-mediated base-editing... Separate expression pRGEB31 - Deliver sgRNA and Cas9 into plants by Agrobacterium-mediated transformation...Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated...

...mE10 CARD11 caspase recruitment domain family, member 11 BIMP3, CARMA1, MGC133069 CBL Cas-Br-M (murine...retroviral transforming sequence C-CBL, CBL2, RNF55 CBLB Cas-Br-M (murine) ecotropic retroviral transforming sequence...DKFZp779F1443, FLJ36865, FLJ41152, Nbla00127, RNF56 CBLC Cas-Br-M (murine) ecotropic retroviral transforming sequence...BRAF1, FLJ95109, MGC126806, MGC138284, RAFB1 CASP3 caspase 3, apoptosis-related cysteine peptidase CPP32...ASBT, ISBT, NTCP2 TAP1 transporter 1, ATP-binding cassette, sub-family B (MDR/TAP) ABC17, ABCB2, APT1, D6S114E...TAP1*0102N, TAP1N TAP2 transporter 2, ATP-binding cassette, sub-family B (MDR/TAP) ABC18, ABCB3, APT2, D6S217E...IMD1, MGC126261, MGC126262, PSCTK1, XLA CARD11 caspase recruitment domain family, member 11 BIMP3, CARMA1...

...typically arise spontaneously, although in rare cases Rett syndrome can be inherited. In females, random...R306C) account for almost 46% of all Rett syndrome cases. Research Milestones 1992 - MECP2 is discovered ...Knock-in of NLuc-tdTomato at endogenous MECP2 locus Castaneus MECP2-NLuc-tdTomato mouse reporter cell line (...resources for a full selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples of additional...hand use in girls: Rett’s syndrome: report of 35 cases. Ann Neurol . 14, 471–479. (Link opens in a new ...