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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 88 results for: IRE

Showing: 51 - 60 of 88 results

  1. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...(loxP) are on the same DNA strand in opposite directions, recombination results in an inversion and the...are on the same DNA strand (in cis) in the same direction, the sequence between the sites is excised as ...evaluating infection/transfection efficiency, selecting desired clones, and making transgenic mice. ID Plasmid ...

  3. CRISPR Plasmids - Single-Strand Break (Nick)

    Type
    Collection
    ...nicks create a double-strand break (DSB) that is repaired using error-prone non-homologous end joining (...template to introduce specific edits via homology-directed repair (HDR). Want more information on the wide...

  4. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...provides a stark contrast to Cas9 and Cpf1, which require that each DNA target have high sequence identity...to limit the spread of infection throughout the entire population. Type VI enzymes that function in mammalian...

  5. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ...living cells. Visualizing a specific genomic locus requires recruitment of many copies of labeled proteins...gRNAs that tile the locus. Chromosome painting requires delivery of hundreds of gRNAs with target sites...

  6. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...purification methods are more straightforward and enable direct identification of molecules associated with a genomic...CRISPR-based purification, design your gRNA sequence to direct dCas9 to a specific locus, avoiding known transcription...

  7. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ...present, the cell may repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental...

  8. CRISPR Plasmids - Parasites

    Type
    Collection
    ...gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ...present, the cell may repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental...

  9. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...that utilize Golden Gate cloning to insert the desired guide RNA sequence by digestion of the backbone...Otx2 expression in the developing mouse retina requires a unique enhancer and either Ascl1 or Neurog2 ...

  10. TALEN Engineering

    Type
    Collection
    ... engineering TAL effector repeat arrays of any desired length: the REAL (Restriction Enzyme And Ligation...assembly process or lists of plasmid ID numbers required for FLASH assembly. Links to additional pages ...
Showing: 51 - 60 of 88 results