...MDH. For detailed clone information, please see the MDH Protein Clone Information Sheet, available to download...window) and contact the hub director for more information. Why MDH? MDH is stable, inexpensive to assay...techniques. Using a single protein with many available isoforms and mutants provides a shared experience for the...mentor, and more. References for teachers Using Bioinformatics and Molecular Visualization to Develop Student...

...into an expression vector of their choice and transforming into E. coli bacteria. This can help reduce ...genetic engineering and directed evolution. More information about the collection can be obtained at the Open... in this collection is ampicillin. Find more information on the enzymes and protocols for expression at...an expression vector of their choice and then transform into E. coli bacteria for expression and purification...Uracil DNA glycosylase 165541 pOpen-Fpg Fpg Formamidopyrimidine DNA glycosylase 165550 pOpen-T4 PGD T4 PGD...

...plasmid ( Addgene #157970 ). Viral preparations are formulated in one of the following three buffers. All buffers...was developed by Shannon Boye’s lab. Plasmid Information The plasmid below is available in each of the...Please click on the plasmid name for ordering information. ID Name Promoter Description Category PI 157970...(CBA) GFP Control Sergei Zolotukhin Citation Information AAV2(Y444F) When using the AAV2(Y444F) serotype...know what you would like to see available in viral format. Please note this does not guarantee viral service...

... Lentiviruses Viral Vector Packaging Service Information Production : Our production process and quality...viral vector production at Addgene, ordering information, and more. Additional Viral Vector Resources ...from the research community demonstrating AAV performance under various experimental conditions. Protocols...institutional biosafety office and your lab, information on the biosafety of viral vectors, and general...

...scientists in the field to assemble the reagents and information you need to use CRISPR technology in your own...Resources CRISPR Guide : Essential background information on CRISPR and the basics for planning your first...editors precisely edit DNA without promoting DSB formation. Nick Mutated Cas nickase enzymes generate single-strand...to reverse transcriptase install new genetic information into a specific DNA site Transcriptional Regulation...

...Information on Addgene's AAV Made on Request viral packaging service...plasmids deposited at Addgene. We are unable to perform custom AAV preparations for specific gene inserts...varies by location. Please contact us for more information. Standard Quality Control Includes: Titering ...next generation sequencing Ordering For more information, to obtain a price quote, or to place an order...

... Collection Technology Plasmids Supplemental Information Background New England Biolabs offers non-toxic...labeling in a sample. Please see the note below for information on substrates and synthases needed for use of...Control for cell surface localization Supplemental Information SNAP and CLIP tags SNAP and CLIP tag FAQs 252.2... Bergendahl V, Marahiel MA.1998. Peptide bond formation in nonribosomal peptide biosynthesis. Catalytic...

...genetic information, viruses are a powerful tool for the delivery of this genetic information to cells.... Viruses facilitate the delivery of genetic information to hard-to-transfect mammalian cells. In addition...of different types of viruses. More detailed information can be found in the guides for each virus type...

...allow multiple Cas9-mediated activities to be performed simultaneously within individual cells. Because... efficient cutting and nicking in bacteria but performs poorly as a transcriptional repressor. All genes...unfavorable bases at positions adjacent to those formally required can significantly reduce activity, while...NNNNGNNT, just as well as the PAM determined by bioinformatics, NNNNGATT. However, it is possible that NNNNGATT...

...Addgene's blog on Systemic Capsids . To review performance data of each of these serotypes, please refer...538421) to cross the blood brain barrier. For information on whether the PHP.eB serotype will work in your...pAAV-CAG-GFP CAG GFP Control Boyden Citation Information When using the Caltech serotypes in future publications...know what you would like to see available in viral format. Please note this does not guarantee viral service...

...CRISPR plasmids The Joung Lab has developed three platforms for engineering TAL effector repeat arrays of ...al., Nat Biotechnol 2012). All three of these platforms assemble TAL effector repeat arrays on a single...Maeder et al., Nat Methods 2013) More detailed information about REAL, REAL-Fast and FLASH, including detailed...

...been cloned, expressed and validated in recombinant form. The recombinant monoclonal antibodies or R-mAbs...IgG2a 149452 Anti-Kirrel3, short isoform 3 [N320/48R] Kirrel3, short isoform 3 Mouse Mouse IgG2a 149453 Anti-Kv4.2...IgG2a 188208 Anti-FGF13/FHF2, B isoform [N225A/10R] FGF13/FHF2, B isoform Human Mouse IgG2a 188209 Anti-...190541 FGF13/FHF2, B isoform scFv [N225A/10] N225A/10 scFv FGF13/FHF2, B isoform Human Mouse 190542 Pan-Shank...

...Please see the following references for more information: Large-scale screening for novel low-affinity...18296487 A benchmarked protein microarray-based platform for the identification of novel low-affinity extracellular...

...Please see the following references for more information: Large-scale screening for novel low-affinity...Integrated Expression) an online database of information on protein interactions generated using AVEXIS...

...the attachment site of the phage 186. Detailed information about the repression system can be found in this...PubMed PMID 29519933 Preprint at bioRxiv Detailed information about chromosomal integration of pLC97 can be...

...programmable endonucleases that can be used to perform targeted genome editing. RGNs consist of two components...needed to construct gRNA expression vectors. More information about RGNs and CRISPR-Cas systems can also be...

...Plasmids and Chemogenetics Guide pages for more information about this field. Receptor Activating hM3D(Gq...know what you would like to see available in viral format. Please note this does not guarantee viral service...

... epeats) pathway, as an RNA-guided DNA binding platform, to repress expression of arbitrary genes in bacteria...described in: Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression...

...Plasmids and Optogenetics Guide page for more information about this field. Use the table and filters below...know what you would like to see available in viral format. Please note this does not guarantee viral service...

... vivo testing of these viral preparations was performed by the Karpova lab. Results for our quality control...know what you would like to see available in viral format. Please note this does not guarantee viral service...