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Showing: 1 - 15 of 15 results

  1. Ras Pathway

    Type
    Collection
    ...Neuroblastoma RAS viral (v-ras) oncogene homolog RASA RASA1 RASA2 RASA3 RAS p21 protein activator RASAL RASAL1...RASAL1 RASAL2 RASAL3 RAS protein activator like RASGRF RASGRF1 RASGRF2 Ras protein specific guanine nucleotide...factor RASGRP RASGRP1 RASGRP2 RASGRP3 RASGRP4 RAS guanyl releasing protein RASSF RASSF1 RASSF2 RASSF3 RASSF4...of plasmids for the Ras pathway. Ras is a small GTPase and constitutively active Ras is the most common... Cancer Research Ras Pathway Ras Pathway You May Also Like: Ras Pathway Clone Collection 2.0...p53 Pathway Background Ras Pathway Plasmids Ras Gene List Resources Background Ras is a small GTPase and...RCE1 Ras converting CAAX endopeptidase 1 RHEB Ras homolog enriched in brain RHO RHOA RHOB RHOC Ras homolog...

  2. Immunology Research Plasmids and Resources

    Type
    Collection
    ...oncogene homolog C-K-RAS, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS3, RASK2 LAT linker for...-Jun KRAS v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog C-K-RAS, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B...homolog C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, K-RAS, N-RAS, RASH1 ICOS inducible T-cell... H-RASIDX, HAMSV, HRAS1, K-RAS, N-RAS, RASH1 IFITM1 interferon induced transmembrane protein 1 (9-27) ...NFAT3 NRAS neuroblastoma RAS viral (v-ras) oncogene homolog ALPS4, N-ras, NRAS1 PAK1 p21 protein (Cdc42... IKBE NRAS neuroblastoma RAS viral (v-ras) oncogene homolog ALPS4, N-ras, NRAS1 PAK1 p21 protein (Cdc42...

  3. p53 Pathway

    Type
    Collection
    ...Damage-specific DNA binding protein 2, 48kDa p53 Tumor protein p53 p53AIP1 Tumor protein p53 regulated apoptosis...Avantaggiati ML, Ogryzko V, Gardner K, Giordano A, Levine AS, Kelly K. Nature. 1997 Jun 27;89(7):1175-84...May Also Like: Cancer Pathway ORF Kit mTOR pathway Ras pathway Background p53 Pathway Plasmids p53 Gene ...angiogenesis inhibitor 1 Bax BCL2-associated X protein Bid BH3 interacting domain death agonist CASP3 ...finger and WD repeat domain 2 (RFWD2); E3 ubiquitin protein ligase cytoC Cytochrome c DR5 Tumor necrosis factor...gamma IGF-BP3 Insulin-like growth factor binding protein 3 KAI CD82 molecule Maspin Serpin family B member...member 5 MDM2 MDM2 proto-oncogene, E3 ubiquitin protein ligase MDMX p53 regulator; also known as MDM4 Noxa...

  4. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...Also Like... CRISPR Guide CRISPR Protein Tagging Fluorescent Protein Guide Subcellular Localization Plasmids...junctions 107579 RAB5A-mEGFP AICSDP-39 mEGFP Ras-related protein Rab-5A Endosomes 107580 AAVS1-mTagRFPT-CAAX...mTagRFPT-CAAX AICSDP-42 mTagRFPT CAAX domain of K-Ras Plasma Membrane 114403 LMNB1-mTagRFP-T AICSDP-29 mTagRFPT... FUS-mEGFP AICSDP-64 mEGFP RNA-binding protein FUS Paraspeckles/stress granules 114411 TNNI1-mEGFP AICSDP...deposited plasmids to generate fluorescently tagged proteins of key cellular structures in human cell lines... will be able to generate fluorescently tagged proteins of key cellular structures in human cell lines...structures. Addgene ID Plasmid Allen Institute ID Tag Protein Structure 87420 PXN-EGFP AICSDP-1 EGFP Paxillin...

  5. CRISPR Guide

    Type
    Collection
    ...naturally-occurring Acr (Anti-CRISPR) proteins can help control CRISPR activity. Acr proteins have evolved in phages ...; or inducing degradation of the Cas9 protein. Most Acr proteins are specific to a particular type of ...and antibody-activator effector proteins; long-term imaging of proteins in living cells (Figure 9B) dCas9...with different fluorescent proteins or by fusing the gRNAs to orthogonal protein-interacting RNA aptamers...applicability of base editing through protein engineering and protein delivery. Nature Communications . 8... by anti-CRISPR proteins. Nature , 526 (7571), 136–139. PMID: 26416740 Bravo, J. P. K., Liu, M., Hibshman...see Cas3 for more details), while type V-K systems use a type V-K Cas effector, Cas12k. No matter the system...

  6. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...128612 Anti-Kv2.1 K+ Channel [D3/71R] Kv2.1 K+ Channel Rat Mouse IgG2a 128613 Anti-Kv1.4 K+ Channel [K13/...K13/31R] Kv1.4 K+ Channel Rat Mouse IgG2a 128614 Anti-Kv1.2 K+ Channel [K14/16R] Kv1.2 K+ Channel Human ...Anti-Kvbeta2/KCNAB2 K+ Channel [K17/70R] Kvbeta2/KCNAB2 K+ Channel Mouse Mouse IgG2a 128616 Anti-Kv1.1 K+ Channel...78R] Kv1.1 K+ Channel Rat Mouse IgG2a 128617 Anti-Pan-Kvbeta K+ Channel [K25/73R] Pan-Kvbeta K+ Channel ...128627 Anti-Kv4.3 K+ Channel [K75/41R] Kv4.3 K+ Channel Rat Mouse IgG2a 128628 Anti-Kv2.1 K+ Channel [K89/...140068 Anti-Kv1.6 K+ channel [K19/36R] Kv1.6 K+ channel Rat Mouse IgG2a 140069 Anti-Kv1.5 K+ channel [K7/45R...Kv1.5 K+ channel Rat Mouse IgG2a 140070 Anti-Kvbeta1.1/KCNAB1 K+ channel [K9/40R] Kvbeta1.1/KCNAB1 K+ channel...

  7. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...re-exposure. More specifically, specialized Cas proteins snip foreign DNA into small fragments approximately...stretches of DNA known as CRISPR arrays. Separate Cas proteins then express and process the CRISPR loci to generate... varying PAM sequences and number/types of Cas proteins. Makarova et al. ’s classification defines 5 types... to DNA. Others inhibit the recruitment of Cas proteins to the CRISPR-Cas complex. Recently, researchers... can include mutations seen in human patients, protein tags, or loxP/FRT sites, among others. Homology-directed...Fluorescently labeled dCas9 or gRNAs that bind fluorescent proteins can be used to image genomic loci in live cells...used to purify a genomic locus and its associated proteins or RNAs through ChIP (enChIP) or biotin-streptavidin...

  8. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...complementary protein function or localization. ACP and MCP tags ACP- and MCP-tags are small protein tags based... labeling cell-surface proteins, and are useful for labeling secreted proteins with disulfide bridges ... H, Johnsson N, Johnsson K. 2004. Specific labeling of cell surface proteins with chemically diverse compounds... Krumm H, Johnsson K, Johnsson N. 2005. Multicolor imaging of cell surface proteins. J Am Chem Soc. 127...the human O6-alkylguanine-DNA-alkyltransferase (hAGT), a DNA repair protein, and is suitable for both cell-permeant...ACP or MCP tagged plasmids for visualization of proteins inside cells. Depositor...Information New England Biolabs offers non-toxic protein labeling systems based on SNAP-, CLIP-, ACP-, and...

  9. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ... independently. The RNA-programmed DNA-binding protein dCas9 is expressed along a CRISPR array that contains...Priest, D. G., Endy, D., Dodd, I. B., & Shearwin, K. E. (2013). One-Step cloning and chromosomal integration... a gene, it blocks the progression of the RNA polymerase, thus strongly decreasing the expression level...

  10. COVID-19 Resources

    Type
    Collection
    ...step is the priming of the S protein by host cell proteases. The S protein of SARS-CoV-2 is primed by the...SARS-CoV-2 nucleocapsid protein (Brian Geiss). Anti-SARS-CoV-2 Nucleocapsid Protein [mBG86] - Clone mBG86...cleaves precursor proteins to a biologically active state. The SARS-CoV-2 S protein contains a potential...Wang, K et al, 2020, BioRxiv SARS-CoV-2 invades host cells via a novel route: CD147-spike protein . Cathepsin...transmembrane glycoprotein of the immunoglobulin superfamily, binds to the SARS-CoV-2 S protein and is involved...CoV, depends on binding of the viral spike (S) protein to cellular receptors. The cellular receptor for...antibodies that target SARS-CoV-2 nucleocapsid protein. SARS-CoV-2 Plasmids : Plasmids that are available...

  11. Tetracycline Inducible Expression

    Type
    Collection
    ...tetR with the C-terminal domain of VP16 (virion protein 16), an essential transcriptional activation domain...effectors for Tet transregulators. Krueger C, Pfleiderer K, Hillen W, Berens C. Biotechniques . 2004 Oct;37(4...additional selection options third-generation rtTA On Fraser 44012 pInducer20 Lentiviral vector for shRNA expression...single copy in mammalian cells; Expresses firefly luciferase hairpin and GFP under pTREtight promoter None...pSBtet-GP Sleeping Beauty transposon system; has luciferase in cloning site; see article for additional selection.... Bockamp E, Maringer M, Spangenberg C, Fees S, Fraser S, Eshkind L, Oesch F, Zabel B. Physiol Genomics...

  12. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Anti-Kv1.1 K+ channel [K20/78R] KCNA1 CMV Episodic ataxia James Trimmer 128627 Anti-Kv4.3 K+ channel [...Anti-Kv1.1 K+ channel [K20/78R-2b] KCNA1 CMV Episodic ataxia James Trimmer 206631 Anti-Kv4.3 K+ channel ...Anti-Kv1.1 K+ channel [K20/78R-1] KCNA1 CMV Episodic ataxia James Trimmer 206684 Anti-Kv4.3 K+ channel ...type, mutations, and/or tags such as fluorescent protein (FP) tags. Information on genes and associated ...ALS Rajat Rohatgi 107856 pHBS1052 TDP43 RRM-GFP R-K TARDBP CMV ALS Rajat Rohatgi 107857 pHBS952 TARDBP-Exon1...CMV ALS Rajat Rohatgi 118796 pHBS1388 TDP43 RRM-GFP K-R TARDBP CMV ALS Rajat Rohatgi 118797 pHBS1147 GFP-TDP43...LRRK2 CMV Parkinson's James Trimmer 177537 Anti-Kv3.3 K+ channel [N375/67R] KCNC3 CMV Spinocerebellar ataxia...

  13. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ... Backbones Fluorescent Protein Guide: Empty Backbones More Fluorescent Protein Resources: FRET Biosensors...fusion proteins with your gene of interest. These are organized By Color of the fluorescent protein and ...The original green fluorescent protein (GFP) was cloned in 1992 ( Prasher et al., Gene, 1992 ), and since...create fusion proteins with your gene of interest. Special... Special Collections Fluorescent Protein Guide Empty ...Imaging Blog: Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs Blog: When is a Monomer not...not a Monomer? Background Fluorescent proteins are genetically encoded tools that are used extensively...

  14. Cre-lox system

    Type
    Collection
    ...Promoter Expression System PI 8394 p209 pCMV-cre-K Cre-K CMV Mammalian Green 8395 p210 pCMV-CREM CREM CMV...specific system for controlling gene expression. The protein Cre recombinase recognizes 34 bp loxP sites, and...fusion; for the production of VLPs loaded with CRE protein CMV Mammalian Ohlmann 120279 014LP-pAAV-ANF-657...stochastic choice of expression among fluorescent proteins, resulting in the indelible marking of mouse neurons...Zeng 61573 Rasgrf2-2A-dCre targeting vector DHFR-destabilized Cre; targeting vector for Rasgrf2 Mammalian...backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR Cre, luciferase, and sgRNA expression EFS AAV Zhang 60229 AAV...Retroviral Luikart 67503 pF CAG luc-EGFP-cre puro Luciferase - EGFP- Cre fusion CAG Mammalian Stringer 68448...

  15. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ... Collections Fluorescent Proteins Fluorescent Biosensors Fluorescent Proteins: Biosensors Metal Ions Small...-Aequorin Protein) for imaging of Ca+ dynamics in endoplasmic reticulum GFP-Aequorin Protein Sensor for... Red fluorescent protein-based cAMP indicator (Pink Flamindo) Red fluorescent protein-based cAMP indicator...of Redox-sensitive protein sensors (roGFP) Lifetime imaging of a fluorescent protein sensor reveals surprising... (Constitutive or Cre-dependent) Ultrasensitive fluorescent proteins for imaging neuronal activity. Nature...Service: AAV Biosensors Fluorescent biosensors are proteins designed so that their fluorescent properties ...in signal of a circularly permuted fluorescent protein (cpFP) or Förster resonance energy transfer (FRET...
Showing: 1 - 15 of 15 results