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Showing: 21 - 40 of 48 results

  1. Optogenetics AAV Preps

    Type
    Collection
    ...options. Browse all Control AAV *AAVrg = retrograde serotype, produced with the AAV retro helper plasmid from...high quality AAV preps from select plasmids in the repository. Browse our optogenetics AAV collection, ... Viral Service AAV Optogenetics Viral Service: Optogenetics AAV Optogenetic tools can be used...20071 pACAGW-ChR2-Venus-AAV CAG ChR2 Venus Constitutive 1, 9 Svoboda 83898 pAAV-mDlx-ChR2-mCherry-Fishell... 28017 AAV-CAG-hChR2-H134R-tdTomato CAG ChR2/H134R tdTomato Constitutive rg* Svoboda 75470 pAAV-CAG-FLEXFRT-ChR2...62723 pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] Syn ChrimsonR tdTomato Cre dependent 5 Boyden 130909 AAV-CAG-...and variants 22222 AAV-FLEX-Arch-GFP CAG Arch GFP Cre dependent 1, 9 Boyden 28305 pAAV-FLEX-ArchT-tdTomato...

  2. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...Wilson AV-9-PV2453 45185-AAV9 AAV-EF1a-BbTagBY Joshua Sanes AV-9-PV2454 45186-AAV9 AAV-EF1a-BbChT Joshua Sanes...18917-AAV1 AAV-FLEX-rev-ChR2-tdtomato Scott Sternson AV-1-20071P 20071-AAV1 pACAGW-ChR2-Venus-AAV Karel Svoboda...18917-AAV5 AAV-FLEX-rev-ChR2-tdtomato Scott Sternson AV-5-20071P 20071-AAV5 pACAGW-ChR2-Venus-AAV Karel Svoboda...18916P 18916-AAV9 AAV-FLEX-rev-ChR2(H134R)-mCherry Scott Sternson AV-9-18917P 18917-AAV9 AAV-FLEX-rev-ChR2... AV-5-PV2432 22222-AAV5 AAV-FLEX-Arch-GFP Ed Boyden AV-1-ALL864 51503-AAV1 AAV pCAG-FLEX-tdTomato-WPRE...ALL854 51502-AAV1 AAV pCAG-FLEX-EGFP-WPRE Hongkui Zeng AV-1-PV0102 105531-AAV1 pAAV.CMV.LacZ.bGH James M. ...51502-AAV2 AAV pCAG-FLEX-EGFP-WPRE Hongkui Zeng AV-2-PV0101 105530-AAV2 pAAV.CMV.PI.EGFP.WPRE.bGH James...

  3. Viral Vectors

    Type
    Collection
    ... Adeno-associated Virus (AAV) Plasmids | Guide Adeno-associated viruses (AAVs) are commonly used for gene...not permanently integrated into the host genome. AAVs are limited by their small packaging capacity. Adenovirus...research goals . For example, adeno-associated virus (AAV) is typically preferred for in-vivo studies because...Stable RNA <8 Kb 80-130 Dividing/Non-dividing Yes Low AAV Transient or Stable* single stranded linear DNA ∼...<8 Kb 80-130 Dividing Yes Moderate * Recombinant AAV has a low frequency of target cell genome integration...

  4. Chemogenetics Plasmids

    Type
    Collection
    ...Sternson 119741 AAV SYN flex PSAM4 GlyR IRES EGFP PSAM4-GlyR Syn IRES-EGFP Yes Sternson 119742 AAV SYN PSAM4...Roth 70717 AAV-mOXT-hM3Dq-mCherry-WPRE hM3D (Gq) Mouse Oxycotin mCherry No Geschwind 66795 pAAV-PTRE-tight-hM3Dq-mCherry...Sternson 119744 AAV CAMKII PSAM4 GlyR IRES EGFP PSAM4-GlyR CamKII IRES-EGFP No Sternson 196041 pAAV Syn intron...Chemogenetics Guide Viral Service: Chemogenetics AAV Blog: Chemogenetics Viral Vectors 101 Blog: Which...Cre- or Flp- dependent. We also offer ready-to-use AAV preparations of select chemogenetic plasmids; these...Recombinase Cre-dependent Flp-dependent Plasmid type AAV Mammalian expression, non-viral Viral Service Available...2a-hM4Dnrxn hM4Dnrxn (Gi) CAG mCherry No Sternson 52521 AAV-CAG::FLEX-rev:: ChR2HA-2a-hM4D hM4Dnrxn (Gi) CAG ...

  5. Luciferase Plasmids

    Type
    Collection
    ...William Hahn, David Root 105533 pAAV.CMV.Luc.IRES.EGFP.SV40 Firefly CMV AAV expression of firefly luciferase...luciferase with a V5 tag Kevin Janes 83281 pAAV-CAG-FLuc Firefly CAG AAV expression of firefly luciferase Mark...Mark Kay 105532 pAAV.CMV.ffLuciferase.SV40 Firefly CMV AAV expression of firefly luciferase James Wilson...transfection Sidi Chen 118412 ssAAV-EF1a-FLuc-WPRE-HgHpA_bac_293 Firefly EF1α AAV expression of firefly luciferase...luciferase Rudolf Jaenisch 105538 pENN.AAV.TBG.PI.ffLuciferase.RBG Firefly TGB AAV expression of firefly luciferase...luciferase Paul Schulze-Lefert 83282 pAAV-CAG-RLuc Renilla CAG AAV expression of renilla luciferase Mark...expressing luciferase. We also offer ready-to-use AAV preparations of select luciferase expression plasmids...

  6. Plasmids for Optogenetics Research

    Type
    Collection
    ...Research You may also like... Optogenetics Guide AAV Viral Service Biosensors Chemogenetics Plasmids Chemogenetics...Looking for viral preps? We also offer ready-to-use AAV preparations of many optogenetics plasmids. ID Plasmid...

  7. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...pX601 61591 Mammalian/AAV BsaI yes, cut S. aureus Zhang pX602 61593 Mammalian/AAV BsaI yes, cut S. aureus...pyogenes Joung AAV:ITR-U6-sgRNA (Backbone) PCB-FlPO-WPRE-syntetisk pA-UTR 68347 Mammalian/AAV none S. pyogenes...pyogenes mCherry Kuhn AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR 60229 Mammalian/AAV SapI none S. pyogenes...pyogenes Zhang AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR 60231 Mammalian/AAV SapI none...pyogenes Zhang AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR 60226 Mammalian/AAV SapI none S...particular expression system , eg. Mammalian, Lentiviral, AAV, Bacteria, Drosophila, Yeast, Plant, Xenopus, Worm... none S. pyogenes Gersbach PX552 60958 Mammalian/AAV SapI none S. pyogenes Zhang pgRNA-humanized 44248...

  8. Neurodegeneration Research Collection

    Type
    Collection
    ... Nat Biotechnol. 2023 May 22. See More AAV Viral Preps Find AAV viral preps for systemic delivery of viral...tauopathy. Ayoub et al. PLoS Genet. 2023 Mar 27. Use AAV expression of GFP and human α-Synuclein to study ...brain cells and more. New and Noteworthy: AAV.rTH.PI.Cre.SV40 ready-to-use viral prep useful for dopamine...

  9. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...capabilities but are small enough to be packaged in AAV particles. Want more information on the wide variety...

  10. Plasmid Collections

    Type
    Collection
    ...Viral Plasmids Lentivirus Adeno-associated Virus (AAV) γ-Retrovirus Adenovirus All Viral Plasmids Curated...

  11. Distribution to Industry

    Type
    Collection
    ...Carrier Proteins (SLCs) from the RESOLUTE collection AAV Packaging from Addgene Parkinson's Research from ...

  12. CRISPR Guide

    Type
    Collection
    ... screens using CRISPR AAV transduction CRISPR elements are inserted into an AAV transfer vector and used...used to generate AAV particles (for details, see our AAV Guide ) ∼4.5 kb packaging limit (only compatible...capabilities and are small enough to be packaged in AAV particles. Browse Plasmids: RNA Editing Activation...Mammalian CRISPR libraries have also been created in AAV backbones for in vivo experiments and in a retroviral...efficiently packaged into adeno-associated virus (AAV) . Since the coding sequence for Staphylococcus aureus... SpCas9, SaCas9 can be efficiently packaged into AAV. Similar to SpCas9, the SaCas9 endonuclease is capable.../or gRNA Infects dividing and non-dividing cells AAV is the least toxic method for in vivo viral delivery...

  13. Adenovirus Plasmids

    Type
    Collection
    ...Adenovirus Guide Virus Biosafety Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook Adenoviruses are...

  14. Retrovirus Plasmids

    Type
    Collection
    ...γ-Retrovirus Guide Biosafety Guide Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook γ-Retrovirus (gamma-retrovirus...

  15. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...David Liu U6-pegRNA-H1-nick sgRNA-mCherry Mammalian, AAV hU6 + H1 pegRNA + nicking sgRNA No mCherry Hyongbum...

  16. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Localization Optogenetics Plasmids Viral Service: AAV Biosensors Fluorescent biosensors are proteins designed...associated with the article. We also offer ready-to-use AAV preparations of select plasmids; these are noted ... synaptophysin (Cre or Flp-dependent) Larsen Lab AAV GCaMP6 Plasmids Rylan Larsen Calcium Calcium sensor...Commun. 2018 Oct 25;9(1):4440. Eric Schreiter Calcium AAV expression of photoconvertible fluorescent protein-based...(6363):623-630. Franck Polleux Calcium Astrocyte AAV-mediated expression of jRCaMP1a, a red fluorescent...voltage imaging. Science. 2019 Aug 1. pii: science.aav6416. Eric Schreiter Voltage Green fluorescent voltage...

  17. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ... smaller than SpCas9 and more easily packaged in AAV. Cas9 orthologs also have distinct PAM sites that...also makes it suitable for multiplexing and use in AAV. Scientists have also developed CRISPR editing technologies...

  18. Tetracycline Inducible Expression

    Type
    Collection
    ...pTREtight promoter None Either Elledge 35625 pAAV-Ptet-RFP-shR-rtTA AAV; shRNA cloning vector; for evaluation... interest. tTA Off Sabatini 92099 AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep Bidirectional promoter controls expression...System. Contains homology arms for integration into AAVS1 Genomic Safe Harbor Locus. Tet-On 3G On Doyon 58245...

  20. Deisseroth INTRSECT Collection

    Type
    Collection
    ...targeting strategy that allows adeno-associated virus (AAV)-borne payloads to be expressed in cells based on...55636 pAAV-EF1a-Cre None 55632 pAAV-Ef1a-mCherry-IRES-Cre None 55637 pAAV-EF1a-Flpo None 55634 pAAV-EF1a-mCherry-IRES-Flpo...pAAV-EF1a-mCherry-IRES-Flpo None 55638 pAAV-EF1a-vCre None 55635 pAAV-EF1a-sCre None 55633 pAAV-EF1a-mCherry-IRES-Dre ...Mutations 55641 pAAV-Ef1a-fDIO EYFP Flp 55640 pAAV-Ef1a-dDIO hChR2(H134R)-EYFP Dre 55639 pAAV-Ef1a-fDIO hChR2...Cre 137135 pAAV-Ef1a-oScarlet None 137136 pAAV-Ef1a-Con/Fon-oScarlet Cre AND Flp 137137 pAAV-Ef1a-Con/Foff... Cre 137125 pAAV-Ef1a-sRGECO None 137126 pAAV-Ef1a-Con/Fon-sRGECO Cre AND Flp 137127 pAAV-Ef1a-Con/Foff...hChR2(H134R)-EYFP Flp 126080 pAAV-Ef1a-sCreDIO hChR2(H134R)-eYFP Scre 126081 pAAV-Ef1a-vCreDIO hChR2(H134R...
Showing: 21 - 40 of 48 results