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We narrowed to 37 results for: cre encoding plasmid

Showing: 1 - 20 of 37 results

  1. AAV Molecular Tools

    Type
    Collection
    ...99121 pAAV-ihSyn1-DIO-tTA Cre-dependent and inducible Synapsin promoter (ihSyn) Cre-dependent expression of...Gradinaru 117383 TRE-DIO-eYFP Cre-dependent and Tetracycline-inducible Cre-dependent, Tet-inducible expression...FLEX-tdTomato-T2A-SypEGFP-WPRE Syn-driven, Cre-dependent Cre-dependent expression of cytoplasmic tdTomato...FLEx mGFP-2A-Synaptophysin-mRuby Syn-driven, Cre-dependent Cre-dependent expression of membrane-bound GFP...Serotype PI 124364 pAAV-FLEX-DTR-GFP CBA-driven, Cre-dependent Cre-dependent expression of diphtheria toxin receptor...45580 pAAV-flex-taCasp3-TEVp EF1a-driven, Cre-dependent Cre-dependent, bicistronic expression of designer...Long 160857 pAAV-FLEx-ER-TurboID CMV-driven, Cre-dependent Cre-dependent expression of ER-localized TurboID...

  2. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Genetically Encoded Calcium Indicators (Constitutive or Cre-dependent) jGCaMP8 Fast Genetically Encoded Calcium...Collection of plasmids encoding fluorescent biosensors to measure biomolecules or genes via FRET or other... Biosensors Luciferase Plasmids Subcellular Localization Optogenetics Plasmids Viral Service: AAV Biosensors...Calcium Teal genetically encoded calcium sensor T-GECI Blue-shifted genetically encoded Ca(2+) indicator with...or astrocytes (Constitutive or Cre-dependent) Li Lab GRAB_Ado Plasmids Yulong Li ADP Biosensor for ADP...detection (iAchSnFR) (Constitutive or Cre-dependent) A fast genetically encoded fluorescent sensor for faithful...Sensor (iGABASnFR) (Constitutive or Cre-dependent) A genetically encoded fluorescent sensor for in vivo imaging...

  3. Brain Initiative Collection

    Type
    Collection
    ...-ER2 AAV production plasmid encoding for Archon1 fluorescent voltage reporter, Cre-dependent expression...publications associated with Addgene materials. Plasmids ID Plasmid Description PI Viral Preps Addgene distributes...104052-AAVPHP.V1 pAAV-CAG-DIO-EYFP An AAV genome encoding Cre-dependent expression of the fluorescent protein...PSAM4 GlyR IRES EGFP Chemogenetics Cre-dependent expression plasmid Scott Sternson 119741-AAV9 AAV SYN...PSAM4 GlyR IRES EGFP Chemogenetics Cre-dependent expression plasmid Scott Sternson 119742-AAV5 AAV SYN...expression plasmid Scott Sternson 123308-AAV9 pAAV-hSyn-GRAB_NE1m Expresses the genetically-encoded fluorescent...in combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors...

  4. Lentivirus Plasmids

    Type
    Collection
    ... multiple plasmids. Some plasmids (typically called packaging and envelope plasmids) encode components... genome is encoded by plasmids called "transfer plasmids," which can be modified to encode a wide range... Viral Plasmids Lentiviral Plasmids Lentiviral Plasmids Additional Resources... Pooled Libraries All Viral Plasmids Packaging Plasmids Transfer Plasmids Lentiviruses, a subset of retroviruses...conjunction with the plasmid that encodes the viral genome (typically called the transfer plasmid ). This table...used with 3rd generation transfer plasmids along with a plasmid encoding Rev Trono 12260 psPAX2 2nd Packaging...packaging plasmids that can be used with 2nd and 3rd generation lentivirus technologies. ID Plasmid Generation...

  5. Optogenetics Plasmid Collection

    Type
    Collection
    ... Plasmid Collections Optogenetics Plasmids Optogenetics Plasmid Collection You may ...Switch Plasmids Background Optogenetics plasmids encode proteins that are controlled by light. Many of ...Chemogenetics Plasmids Chemogenetics Guide Fluorescent Protein Guide Microbial Opsin Plasmids Optical Switch...of these plasmids encode for microbial opsins, which are membrane channels and pores derived from algae...Microbial Opsin Plasmids Browse, sort, or search the table below for the optogenetics plasmids for neuronal...ready-to-use AAV preparations of many optogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter... top Optical Switch Plasmids Browse, sort, or search the table below for plasmids that use light to control...

  6. Retrograde AAV viral preps

    Type
    Collection
    ...AAV-pgk-Cre PGK Cre expression Recombinases Aebischer 55632 pAAV-Ef1a-mCherry-IRES-Cre EF1a Cre and physically-separate...Cre.SV40 CamKII Cre expression Recombinases Wilson 107738 pAAV-hSyn-Cre-P2A-dTomato Syn Cre expression with... by using this serotype to deliver Cre-dependent transgenes in Cre transgenic lines. As part of our standard...-EGFP Syn EGFP, Cre-dependent Control Roth 55650 pAAV-hSyn Con/Fon EYFP Syn EYFP, Cre and Flp-dependent...pAAV-FLEX-tdTomato CAG tdTomato, Cre-dependent Control Boyden 27056 pAAV-Ef1a-DIO EYFP EF1a EYFP, Cre-dependent Control...Expresses NLS-mCherry in the absence of Cre, and expresses NLS-EGFP in Cre-positive cells. Control Harvey 137164...expression Recombinases Deisseroth 55636 pAAV-EF1a-Cre EF1a Cre expression Recombinases Deisseroth 55637 pAAV-EF1a-Flpo...

  7. Viral Production

    Type
    Collection
    ...transfer plasmid, a plasmid encoding Rep and serotype-specific Cap genes, and a plasmid encoding adenoviral... the absence of Cre. mCherry expression alone was detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from... coli . In addition, plasmids are typically prepared using endotoxin-free plasmid purification kits. Resources...contamination is minimized by using an endotoxin-free plasmid purification protocol. To minimize the immunogenic...were transduced with either pAAV-Ef1a-mCherry-IRES-Cre (55634-AAVrg) alone at 1.7E6 viral genomes (vg)/cell...) alone at 1.1E6 vg/mL, or both. Two days later, Cre-dependent GFP expression was detected with direct...off-line and discarded. Confirmation of Transfer Plasmid Addgene uses a rigorous barcode matching system...

  8. Zebrafish Plasmid Collection

    Type
    Collection
    ... Plasmid Collections Zebrafish Plasmids Zebrafish Plasmids and Resources... of the plasmids and tools available at Addgene for use in zebrafish. Browse Plasmids Plasmid Tools for...biosensors, and Cre expression plasmids. Large collections or kits are described below . ID Plasmid Description...for plasmids containing your zebrafish gene of interest? Search Addgene's collection for plasmids that...highlights plasmids that contain tools for use in zebrafish research. This includes plasmids for genome...expressed. EXPRESs Plasmids for Zebrafish Receptors and Zebrafish Monoclonal Antibody plasmids - Gavin Wright...Wright Lab. Collections of plasmids that encode zebrafish receptors or monoclonal antibodies to zebrafish...

  9. Recombinases AAV Preps

    Type
    Collection
    ...that encode site-specific recombinases, which can be used to control gene expression. See our Cre-lox ...Cre-lox Plasmids page for more information about these molecular tools. Cre AAV ID Name Promoter Fluorophore...Deisseroth 55636 pAAV-EF1a-Cre EF1a none 11, rg* Deisseroth 121675 pAAV-EF1a-fDIO-Cre EF1a none 1, 5, 8, 9,...107312 AAV-hSyn-mCherry-P2A-Cre-WPRE Syn mCherry 1 Yang 107738 pAAV-hSyn-Cre-P2A-dTomato Syn dTomato 1,...Service: Recombinases Cre AAV Dre AAV Flpo AAV Light-Inducible Recombinases VCre AAV Browse the following....CMV.HI.eGFP-Cre.WPRE.SV40 CMV eGFP 1, 2, 5, 8, 9 Wilson 55632 pAAV-Ef1a-mCherry-IRES-Cre EF1a mCherry...tdTomato 9 Pu 105550 pAAV.GFAP.Cre.WPRE.hGH GFAP none 5, PHPeB Wilson 196410 AAV-GfaABC1D-Cre-4x6T GfaABC1D ...

  10. Deisseroth INTRSECT Collection

    Type
    Collection
    ...Collections INTRSECT Plasmid Collection INTRSECT Plasmid Collection INTRSECT Implementation Plasmids References ...of populations expressing only Cre AND Flp, Cre NOT Flp, or Flp NOT Cre. B) This strategy has been expanded...AND NOT Cre Yes 137129 pAAV-Ef1a-Con/Fon-BFP Cre AND Flp Yes 137130 pAAV-Ef1a-Con/Foff 2.0-BFP Cre AND NOT...intersectional expression plasmids that combine multiplexed recombinases, including Cre, Flp, and VCre to enable viral...expressing Cre AND Flp, here expressed through a double transgenic animal strategy (PV-2a-Cre; SOM-IRES-Flp...novel INTSRECT plasmids for groups interested in producing their own INTRSECT plasmids. Online spreadsheet...genetically-encoded calcium indicators, and rabies targeting genes. Recombinases Addgene ID Plasmid Logic Sites...

  11. Chemogenetics AAV Preps

    Type
    Collection
    ...dTomato Activity Cre-dependent Flp-dependent Cre and Flp-dependent Constitutive (non-cre dependent) Serotype...Ready-to-use AAV available from Addgene's viral service encoding chemogenetics tools. Viral... in specific cell types. See our Chemogenetics Plasmids and Chemogenetics Guide pages for more information...Filters ID Name Receptor and Activity Reporter/Tag Cre requirement Serotype PI 44361 pAAV-hSyn-DIO-hM3D(...Gq)-mCherry hM3D(Gq) - Activation mCherry fusion Cre-dependent 1, 2, 5, 8, 9, rg*, PHPeB Roth 44362 pAAV-hSyn-DIO-hM4D...Gi)-mCherry hM4D(Gi) - Inhibition mCherry fusion Cre-dependent 1, 2, 5, 8, 9, rg*, PHPeB Roth 65417 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine...kappa-opioid receptor (KOR) - Inhibition IRES mCitrine Cre-dependent 8 Roth 50454 pAAV-hSyn-DIO-HA-hM3D(Gq)-...

  12. Zinc Finger Consortium: Nuclease Expression Vectors

    Type
    Collection
    ...This page lists plasmid vectors designed to express nucleases in different cell types. Zinc finger arrays... Plasmid Collections Zinc Finger Consortium Nuclease Expression...Consortium: Nuclease Expression Vectors This page lists plasmid vectors designed to express nucleases in different...these vectors using unique cloning sites. Vectors encoding wild-type FokI nuclease domains: pST1374 (for ...zebrafish) pDW1775 (for expression in plants) Vectors encoding heterodimeric FokI nuclease domains: pMLM290 (...different cell types. Zinc finger arrays created using ZFC reagents for either OPEN or modular assembly can...

  13. Retrovirus Plasmids

    Type
    Collection
    ... and Packaging Plasmids Common γ-retroviral envelope and packaging plasmids. ID Plasmid Type Description...envelope plasmid Kasparov Popular γ-Retroviral Transfer Plasmids Common γ-retroviral transfer plasmids. Want...retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids. Viral...Plasmids γ-Retroviral Plasmids γ-Retroviral Plasmids Additional Resources: γ-Retrovirus Guide Biosafety...Biosafety Guide Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook γ-Retrovirus (gamma-retrovirus) is an...with packaging plasmid Weinberg 12259 pMD2.G Envelope VSV-G-expressing envelope plasmid Trono 1733 pCI-VSVG...envelope plasmid Nienhuis and Salmon 35616 pCAG-VSVG Envelope VSV-G-expressing envelope plasmid Nienhuis...

  14. Zhang Lab CRISPR Page

    Type
    Collection
    ...pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR This plasmid contains two expression cassettes, Renilla luciferase-2A-Cre recombinase...sgRNA(LacZ)-pCBh-Cre-WPRE-hGHpA-ITR This plasmid contains two expression cassettes, Cre recombinase and...(backbone)-pCBh-Cre-WPRE-hGHpA-ITR This plasmid contains two expression cassettes, Cre recombinase and...ITR-U6-sgRNA(NeuN)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR This plasmid enables Cre/loxP recombination and...sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR This plasmid facilitates Cre/loxP recombination ...tracrRNA: This plasmid contains three expression cassettes. To target a given site, the plasmid can be digested... vector encoding dCAS9-VP64 with 2A Blast resistance marker 61426 : lentiviral vector encoding the MS2...

  15. Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection

    Type
    Collection
    ... Zhang Lab Plasmids The Human TFome Library Stem Cells Protocols & References MORF Plasmids MORF Pooled...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...bulk screening (PDF, 85 KB) TF ORF barcode dial out PCR for 10X scRNA-seq (PDF, 59.2 KB) Plasmids ID Plasmid...transcription factor collection that contains 1,836 genes encoded by 3,548 isoforms and two control vectors. The ... Plasmid Gene/Insert Pooled Library The MORF pooled library is a comprehensive collection of human transcription...factor isoform in pooled screens. This collection and library can be used for screening transcription factors...available at Addgene. The collection can be used for screening transcription factors for cellular programming...

  16. TALEN Plasmids and Kits

    Type
    Collection
    ...List of plasmid kits and accessory plasmids available from Addgene for generating your own specific TALE...Yamamoto Additional Plasmids for Use with Addgene TALEN Kits For Use With: ID Plasmid Depositing PI Description...in mammalian cells. These plasmids allow for cloning DNA fragments encoding TAL effector repeat arrays...TALE Toolbox 49622 - 49647 24 plasmids Thoru Pederson TALEColor plasmids: destination vectors for use ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...demethylase) made using these plasmids along with the FLASH method can decrease the amount of Histone H3 lysine... TALE plasmids. Genome...

  17. Chemogenetics Plasmids

    Type
    Collection
    ... Plasmid Collections Chemogenetics Plasmids Chemogenetics Plasmid Collection You may...Chemogenetics vs. Optogenetics Chemogenetics plasmids encode genetically engineered receptors that are activated...Chemogenetics Plasmids Browse, sort, or search this table to find chemogenetics plasmids in our collection...ready-to-use AAV preparations of many chemogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...PSEMs). PSAMs are coupled to an ion pore domain to create a ligand-gated ion channel. Whether a PSAM is activating...

  18. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...SepOTSλ Plasmid 68291 SepOTSκ Plasmid 68290 SepOTSι Plasmid 68289 SepOTSθ Plasmid 68288 SepOTSη Plasmid 68287...S218TAG Plasmid 68300 MBP-MEK1 Plasmid 68299 GFP E17TAG/Q157TAG Plasmid 68298 GFP S2TAG/E17TAG Plasmid 68297...GFP Q157TAG Plasmid 68296 GFP S2TAG Plasmid 68295 GFP E17TAG Plasmid 68294 SepOTSν Plasmid 68292 SepOTSλ...68287 SepOTSζ Plasmid 68286 SepOTSϵ Plasmid 68285 SepOTSδ Plasmid 68284 SepOTSγ Plasmid 68283 SepOTSβ Plasmid....ΔA.Δserb.Amp Plasmid 68305 Beta lactamase S68TAG Plasmid 68302 MBP-MEK1 S222TAG Plasmid 68301 MBP-MEK1...pSerOTS-C1* (V70) (Plasmid #188537) a better choice in place of the popular SepOTSλ (Plasmid #68292) . An important...-C1* (V70) is a ROP minus plasmid and is not compatible with other plasmids containing high copy ColE1...

  19. Plan Your Experiment

    Type
    Collection
    ...design. Addgene offers many plasmids containing validated gRNAs . These plasmids contain gRNAs that have ...cells is to directly transfect expression plasmids encoding your Cas protein and gRNA, which you generally...generate stable cell lines if your plasmid contains a selection marker. Plasmid delivery is versatile thanks...or mRNA encoding the Cas enzyme and gRNA, or as a functional ribonucleoprotein (RNP). Created with BioRender.com...Application Cas Enzyme Additional Considerations Browse Plasmids Knockout Permanently disrupt gene function; cells...different activator systems exist, including the multi-plasmid SAM system Activate Design and Clone Your gRNA ..., anneal, and insert the targeting oligos into plasmids containing the gRNA scaffold using standard restriction-ligation...

  20. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...CRISPR Plasmids: Drosophila Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime...locus and encodes a primer binding site and the desired edits on an RT template. ID Plasmid Gene/Insert...gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter...with a wide variety of Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance... Cas9 plasmid Bullock and Port 51026 U6-BbsI-crRNA dU6 BbsI Transfection none, need Cas9 plasmid O'Connor-Giles...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR plasmids that have been designed for use in Drosophila and other insects. CRISPR...
Showing: 1 - 20 of 37 results