Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 50 results for: crispr cas9 plasmids

Showing: 1 - 20 of 50 results

  1. CRISPR References and Information

    Type
    Collection
    ...Resources CRISPR Protocols Addgene CRISPR Resources CRISPR Plasmids Addgene's collection of CRISPR plasmids... cloning CRISPR RNA array: Cas9 (pX260) or Cas9 D10A (pX334) ; tracrRNA: Cas9 (pX330) or Cas9 D10A (pX335...screening. CRISPR eBook Addgene's downloadable eBook about all things CRISPR. Addgene CRISPR blogs CRISPR tips...inhibition, and barcoding. CRISPR Kits Addgene's collection of CRISPR plasmid kits. CRISPR Software Sanger Indel...sites for S. pyogenes Cas9, S. aureus Cas9, S. thermophilus Cas9, N. meningitidis Cas9, or Cas12a from your...using ssDNA oligos p201G Cas9 ; p201B Cas9 ; p201H Cas9 ; p201N Cas9 ; pUC gRNA Shuttle PDF, 500 KB Sabatini... CRISPR References and Information CRISPR References and Information Addgene Resources CRISPR Software...

  2. CRISPR Guide

    Type
    Collection
    ...Figure 7: Overview of CRISPR transposases Browse Plasmids: CRISPR transposases CRISPR Recombinases and Integrases...genomic regions using CRISPR Browse Plasmids: Purify Cas9 Alternatives for CRISPR Genome Engineering S....References Website CRISPR plasmids and resources at Addgene CRISPR 101 eBook (3rd edition) CRISPR software and...Guide CRISPR Guide Jump To: CRISPR Guide Overview CRISPR Basics Small Edits Large Edits Genome-Wide Screening... containing the CRISPR plasmid will have all desired genomic edits carried out by Cas9. Multiplexing applications...more about Cas9 multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9 Endonuclease... engineering. Read more about Cas9 variants . Anti-CRISPR Prolonged Cas9 activity can increase the risk...

  3. Zhang Lab CRISPR Page

    Type
    Collection
    ...PubMed . Return to AAV In vivo plasmids AAV Plasmids with Cas9 Mouse CRISPR-Cas9 knockin mice for genome editing...backbone cloning information: CRISPR-Cas9 mouse toolbox protocol 637.8 KB CRISPR-Cas9 Cre expression vectors ...Design Tools CRISPR Blog Posts Jump to: SpCas9 Plasmids GeCKO SAM AAV - In vivo Delivery AAV - Cas9 Mouse CRISPR... 24. PubMed . Return to SpCas9 plasmids GeCKO Library Genome-scale CRISPR-Cas9 knockout screening in human...CRISPR Cas9 gRNA plasmids from the Zhang lab Genome... Genome Engineering CRISPR Zhang Lab CRISPR Plasmids Zhang...Zhang Lab CRISPR Plasmids Available from Addgene Zhang Lab CRISPR Resources Tips for and FAQs about the ...

  4. Plan Your Experiment

    Type
    Collection
    ... started using CRISPR in your research. Although we will use the example of CRISPR/Cas9 in mammalian cells...the multi-plasmid SAM system. Select Expression System To use CRISPR, you will need both Cas9 and a gRNA... specific plasmid in question (see CRISPR protocols from Addgene depositors ). Deliver Cas9 and gRNA Choose... Science Guides CRISPR Guide Plan Your Experiment Plan Your CRISPR Experiment Jump To: Plan Your Experiment...organism without a specific preferred mutation Cas9 (or Cas9 nickase) Single (or dual) gRNA targeting 5′ ...generating a point mutation or inserting a tag Cas9 (or Cas9 nickase); Base editor Single (or dual) gRNA ...express the CRISPR machinery. For more difficult cells (e.g. primary cells), viral delivery of CRISPR reagents...

  5. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR revolution shows no...out Addgene’s CRISPR Guide and CRISPR blog posts , or find CRISPR plasmids for your research. Addgene has... CRISPR CRISPR History CRISPR History and Development for Genome Engineering You may also like...sequencing, CRISPR is a robust system for genome-wide screening. CRISPR Technological Advances CRISPR technology...High fidelity Cas9s: Three high fidelity Cas9 variants , SpCas9-HF, eSpCas9, and HypaCas9 display very ...finger nucleases (ZFNs) and TALENs. CRISPR: An Adaptive Immune System CRISPR (Clustered Regularly Interspaced...prokaryotes used CRISPR as part of an adaptive immune system - utilizing various CRISPR-associated (Cas...

  6. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...none, need Cas9 plasmid Boxem 47941 pMB60 T7 BsaI In vitro transcription none, need Cas9 plasmid Boxem 47912... CRISPR C. elegans CRISPR Plasmids: C. ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...following CRISPR plasmids have been designed for use in C. elegans. Cut Fully functional CRISPR/Cas enzymes...Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing...need Cas9 plasmid Joung 46170 PU6::klp-12_sgRNA cU6 PCR (see paper) Transfection none, need Cas9 plasmid... elegans none, need Cas9 plasmid Ristow Do you have suggestions for other plasmids that should be added...

  7. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...CRISPR Plasmids: Drosophila Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing...transcription Virmilion none, need Cas9 plasmid Bullock and Port 49330 pAc-sgRNA-Cas9 dU6 BspQI Transfection Puromycin..., need Cas9 plasmid Bullock and Port 51026 U6-BbsI-crRNA dU6 BbsI Transfection none, need Cas9 plasmid...gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter...CRISPR plasmids that have been designed for use in Drosophila and other insects. CRISPR...

  8. Worm Expression Resources

    Type
    Collection
    ... Plasmid Collections Worm Plasmids Worm Plasmids and Resources Plasmid Tools Worm Constructs... insertion using CRISPR/Cas9 and the selection of C. elegans strains. CRISPR plasmids from John Calarco's...elegans via a CRISPR-Cas9 system. Cloning-free CRISPR for C. elegans , which uses purified Cas9, such as that...some of the plasmids and tools available at Addgene for use in worms. Browse Plasmids Plasmid Tools for ...Addgene's CRISPR Guide for a general introduction to CRISPR technology. Addgene's worm CRISPR resources ...Browse plasmid tools, kits, and resources (such as CRISPR and synthetic biology collections) for use ...feeding worms bacteria expressing dsRNA or use CRISPR/Cas9 gene editing to easily engineer specific transgenic...

  9. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ... Genome Engineering CRISPR Zhang Lab CRISPR Plasmids Zhang Lab CRISPR FAQs Zhang...Zhang Lab's CRISPR Frequently Asked Questions You may also like... Zhang Lab CRISPR plasmids General CRISPR...using the lab's CRISPR technology. Visit the Zhang Lab page and forum to learn more. CRISPR Design FAQs Homologous...the Bench FAQs CRISPR Design FAQs Should I use wildtype or double nickase for my CRISPR genome engineering...type to use for your CRISPR genome engineering experiments, consider that wildtype Cas9 with optimized chimeric...the Cas9 nickase doesn't have the ability to induce double-stranded breaks like the wildtype Cas9 does...multiplexed targeting with Zhang lab plasmids? For multiplexing CRISPR to target multiple genome loci, the...

  10. Plant Plasmids and Resources

    Type
    Collection
    ... Plasmid Collections Plant Plasmids Plant Plasmids and Resources...biology - deposit plant plasmids with Addgene! CRISPR Please refer to Addgene's CRISPR Guide for a general...introduction to CRISPR technology or the Plant CRISPR Resources for a full selection of plasmids expressing... an intronized Cas9 series of vectors and DNA modules for assembly of CRISPR/Cas9 constructs - Sylvestre...like... Plant CRISPR resources Fluorescent Protein Guide Addgene Blog: Plant Biology CRISPR Cloning Transcription...addition to individual plasmids, Addgene carries several useful toolkits and plasmid collections for use ...functional variants of Cas9 and empty gRNA backbones. A few examples of useful plant CRISPR resources include...

  11. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...CRISPR Plasmids: Base Edit Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Two... or search the tables below for CRISPR base editing plasmids. Plasmids are available for expression in...are created by fusing Cas9 nickase or catalytically inactive “dead” Cas9 (dCas9) to a cytidine deaminase...CRISPR plasmids for precisely editing DNA bases without inducing a DNA break. CRISPR...editors are available with multiple Cas9 variants including high fidelity Cas9’s. Further advancements have ...(TAM) from the Chang lab and CRISPR-X from the Bassik lab , in which Cas9 is fused to activation-induced...

  12. CRISPR Plasmids - Xenopus

    Type
    Collection
    ... CRISPR Xenopus CRISPR Plasmids: Xenopus Browse...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The... following CRISPR plasmids have been designed for use in Xenopus. Cut Fully functional Cas9 enzymes designed...Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing...transcription none, need Cas9 plasmid Chen Do you have suggestions for other plasmids that should be added...CRISPR plasmids that have been designed for use in Xenopus. CRISPR... Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation...

  13. Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids

    Type
    Collection
    ...Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids You may also like... CRISPR Guide CRISPR Protocols...of Staphylococcus aureus Cas9 (SaCas9 KKH variant) Engineered CRISPR-Cas9 nucleases with altered PAM specificities...specificities Dimeric CRISPR RNA-guided FokI Nucleases (RFNs) CRISPR-Cas/RGN expression plasmids for use in human...human cells CRISPR-Cas/RGN expression plasmids for use in zebrafish Guide RNA expression plasmids for the ...Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids Genome... Genome Engineering CRISPR Joung Lab CRISPR Plasmids...Tools CRISPR Blog Posts RNA-Guided Nucleases ( RGNs ), based on naturally occurring Type II CRISPR-Cas ...

  14. Lentiviral Prep Service

    Type
    Collection
    ...Root Cas9 Lentivirus Cas9 viruses can be used to interrogate the genome in a variety of ways. Cas9 proteins... list of gRNA plasmids targeting specific genes. For more information on CRISPR plasmids and function,...function, see our CRISPR plasmids resource page . Cas9 for Editing Genomic Sequences To perform genome edits,... 52962 lentiCas9-Blast Sp Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes Cas9 protein...backbone. Zhang Cas9 and Accessories for Activating Gene Expression Catalytically-dead Cas9 (dCas9) can be fused...Lentivirus Browse In-Stock Lentivirus Pooled CRISPR Libraries Cas9 Pooled Barcoding Libraries Control Addgene's...are made from plasmids that are currently in our repository. You can obtain the plasmid from us or receive...

  15. CRISPR Plasmids - Bacteria

    Type
    Collection
    ... CRISPR Bacteria CRISPR Plasmids: Bacteria...following CRISPR plasmids have been designed for use in bacteria. Cut Fully functional CRISPR/Cas enzymes...efficiency than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Browse CRISPR Plasmids By Function Genome...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Base...Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Validated In Resistance Co-expressed Cas9 Depositing...Ampicillin none, need Cas9 plasmid Qi Do you have suggestions for other plasmids that should be added ...gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter...

  16. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Whether the plasmid contains Cas9 and if so, which function of Cas9. We have plasmids with Cas9 that can ... CRISPR Empty gRNA Vectors CRISPR Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR requires...Lab . Plasmid Expression System Cloning Enzyme Co-expressed Cas9 Cas9 System Selection PI pCRISPR 42875... on the plasmid. gRNA plasmids that do not co-express a Cas protein require a separate plasmid that does...from our collection. Plasmids Expression System Description PI Multiplex CRISPR/Cas9 Assembly System Kit...independent U6 promoters and Cas9 from CBh promoter. Ventura A CRISPR/Cas9 toolkit for multiplex genome...

  17. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...the usage of the pSpCas9(BB) plasmid (pX330) (Addgene plasmid ID 42230). This plasmid allows for the simultaneous...Mammalian Cell Lines Other Addgene Resources CRISPR Plasmids CRISPR Guide Addgene Blog Posts Addgene is proud.... Perform maxi-prep for each CRISPR/Cas9 construct. Transfecting CRISPRs into Cells of Interest NOTE: ...: This protocol involves the delivery of CRISPR/Cas9 plasmids by electroporation 4 . This protocol is ...cells. However, transient transfection of CRISPR/Cas9 plasmids may be successfully adapted to numerous...using CRISPR/Cas9 CRISPR... CRISPR References & Information Genomic Deletions in Mammalian Cell Lines CRISPR: Protocol...

  18. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...need Cas9 plasmid Chen and Wente 42250 DR274 T7 BsaI In vitro transcription none, need Cas9 plasmid Joung... CRISPR Zebrafish CRISPR Plasmids: Zebrafish...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...following CRISPR plasmids have been designed for use in zebrafish. Cut Fully functional CRISPR/Cas enzymes...Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing...CRISPR plasmids that have been designed for use in zebrafish. CRISPR...Zebrafish Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...

  19. Zebrafish Plasmid Collection

    Type
    Collection
    ...Using CRISPR/Cas9 - Jeffrey Essner Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) expression plasmids - Keith...Joung Lab CRISPR/Cas9-based conditional mutagenesis in zebrafish - Wenbiao Chen Lab A CRISPR/Cas9 Vector ...modified CRISPR-Cas9 system - Shuo Lin Lab Re-purposed Ac/Ds transgenic system enabling CRISPR/dCas9-interference... Plasmid Collections Zebrafish Plasmids Zebrafish Plasmids and Resources... of the plasmids and tools available at Addgene for use in zebrafish. Browse Plasmids Plasmid Tools for...Collection CRISPR Please refer to Addgene's CRISPR Guide for a general introduction to CRISPR technology... or the zebrafish CRISPR resources for a full selection of plasmids expressing Cas9 and empty gRNA backbones...

  20. CRISPR Plasmids - Tagging

    Type
    Collection
    ...and Myers Tagging Plasmids Protein Species Tag Donor Plasmid gRNA plasmid gRNA plasmid FLAG pFETCh_Donor...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...proteins. This CRISPR/Cas plasmid-system consists of two components: A vector containing Cas9 and a validated...Tagging Plasmids: pX330S-2-PITCh - expresses Cas9 & PITCh-gRNA pX330A-FBL/PITCh - expresses Cas9, PITCh-gRNA...CRISPR plasmids for endogenous tagging of your gene of interest. CRISPR... CRISPR Protein Tagging CRISPR Plasmids...Plasmids - Protein Tagging Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit...
Showing: 1 - 20 of 50 results