Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 42 results for: Dos;

Showing: 21 - 40 of 42 results

  1. Protocol - Bacterial Transformation

    Type
    Protocol
    ...transforming large plasmids (>10 kb) or BACs, what can I do? Chemically competent cells are fast and easy to ...cell/DNA mixture to induce membrane permeability. To do this you will need to have access to an electroporator...

  2. Pipetting Protocol

    Type
    Protocol
    ...box and push the end of the pipette onto the tip. Do not touch the tips with your fingers to avoid contaminating...plunger, gently lower the pipette tip into the liquid. Do not submerge the pipette itself into the liquid and...

  3. Colony Formation Titering Assay

    Type
    Protocol
    ...regularly Do not over or under-grow your cells. Thaw a new vial of cells after 20–30 passages. Do not add...Procedure Before beginning a colony formation assay, the dose of antibiotic required to kill your target cell ...determined. Treat the target cells with a range of doses of antibiotic. Determine the minimum concentration...window) required to kill all of the cells. Use this dose for the colony formation assay. Prepare a batch ...

  4. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...thicker as the proteins and DNA are denatured. Pro-Tip Do not vortex at this stage or the genomic DNA will ...4. Note: Phenol-chloroform is a hazardous waste - DO NOT pour down sink. Top Protocol: Ethanol Precipitation...

  5. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...Media 10 mL 10% Pluronic-F68 40 mL 200 mM Glutagro Do not add selective reagents Store at 4 °C until use...incubator on a shaking platform set to 120 rpm. Pro-Tip Do not use cells that are over 30 passages. Section ...

  6. Protocol - How to Design Primers

    Type
    Protocol
    ...complementary to template strand). However, primers do not need to correspond to the template strand completely...

  8. Weighing Reagents Protocol

    Type
    Protocol
    ...surface. Tare the weighing boat or paper. You need to do this because you don’t want to include the weight...

  9. AAV ddPCR Titration

    Type
    Protocol
    ...entire dilution plate with Microseal adhesive seal - do not press the film, just gently cover so nothing ...collecting drops along the side of the tube. Cap gently - do not push the cap in all the way, just ensure the ...

  11. Water Bath Protocol

    Type
    Protocol
    ...of the tube. This way, as items are floating, you do not need to necessarily maneuver the bottles or tubes...

  13. Video Library

    Type
    Protocol
    ...Protocol Storing and Handling Addgene Plasmids What to do after you receive your plasmids from Addgene Instructions...

  14. Centrifugation

    Type
    Protocol
    ... centrifuge and be dangerous for the user. If you do not have a balanced number of samples, you may need...

  16. Immunocytochemistry

    Type
    Protocol
    ... and a negative control sample such as cells that do not express the protein of interest....titrating your antibody to determine the optimal dose. To ensure that your antibody is both functioning...

  17. General Transfection

    Type
    Protocol
    ...8x10 5 cells in a T75 flask in a volume of 15 mL. Do not add antibiotics to the media. Use cells that ...

  18. Lentivirus Production

    Type
    Protocol
    ...10 5 cells in a T75 flask in 15 mL DMEM Complete. Do not add pen-strep to the media. Use cells that are...

  20. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...purified antigen to demonstrate the expected antibody dose response....probe with an antibody against the target to show a dose response. Sharing speeds science. We believe that...standard curve . If you don’t see any color change or dose response, your antibody concentration may have been... the reaction is saturated and you don't see any dose response, your antibody concentration may have been...
Showing: 21 - 40 of 42 results