Skip to main content
Addgene
Showing: 21 - 40 of 40 results

  1. General Transfection

    Type
    Protocol
    ...linear polyethylenimine. Transfections allow for transient expression of a gene of interest in a target cell...discard the tube and thaw a new working stock. Considerations Before You Start The health of the cell line...

  2. Gibson Assembly Protocol

    Type
    Protocol
    ...and molecular biologists now use this method extensively. Why Gibson Cloning? No need for specific restriction... Assembly master mix (isothermal reaction mix) consists of three different enzymes within a single buffer...

  3. Immunocytochemistry

    Type
    Protocol
    ...well. Allow the HeLa cells to grow to the desired density before labeling. Section 2: Fixing and permeabilizing...instructions before starting your experiment and consider titrating your antibody to determine the optimal...

  4. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ... 96-well optical plate Pipette tips General Considerations Always run standards and samples in duplicate... #59462 is one plasmid that gave reliable and consistent results. Use the recommended plasmid, or test...

  5. Pouring LB Agar Plates

    Type
    Protocol
    ... bath Pipetman Reagents 37 g pre-mixed powder consisting of: 5.0 g yeast extract 10.0 g peptone from casein...colonies will grow). However, it is also more expensive. Procedure Measure 37g of pre-mixed LB-agar powder...

  6. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...After staining with Coomassie blue, protein band intensities are measured using ImageJ software and a standard...similar photo software to determine the relative intensity of the protein bands to the overall lane. Import...

  7. Western Blot

    Type
    Protocol
    ...blocking buffer and optimal antibody concentrations. Consider titrating your antibody to determine the optimal...instructions before starting your experiment and consider titrating your antibody to determine the optimal...

  8. CRISPR Library Amplification

    Type
    Protocol
    ... (QC) Pooled libraries can be challenging and expensive to ensure adequate quality, but this upfront cost...for all plates. Use two 10 mL pipettes (one for dispensing cold LB and one for removing LB containing bacteria...

  9. DNA Quantification

    Type
    Protocol
    ...such as TE). You will get much more accurate and consistent readings from DNA in a buffered solution. Reference...

  10. Protocol - How to Design Primers

    Type
    Protocol
    ...for broader pairing capabilities. Taking into consideration the information above, primers should generally...

  16. Lentivirus Production

    Type
    Protocol
    ...batch of 1 mg/mL PEI and for each cell line. Considerations Before You Start The health of the packaging...
Showing: 21 - 40 of 40 results